{"results":[{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"O96009","secondaryAccessions":["Q8WWD9"],"uniProtkbId":"NAPSA_HUMAN","entryAudit":{"firstPublicDate":"2000-05-30","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1999-05-01","entryVersion":186,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Homo sapiens","commonName":"Human","taxonId":9606,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Primates","Haplorrhini","Catarrhini","Hominidae","Homo"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Napsin-A"},"ecNumbers":[{"value":"3.4.23.-"}]},"alternativeNames":[{"fullName":{"value":"Aspartyl protease 4"},"shortNames":[{"value":"ASP4"},{"value":"Asp 4"}]},{"fullName":{"value":"Napsin-1"}},{"fullName":{"value":"TA01/TA02"}}],"flag":"Precursor"},"genes":[{"geneName":{"value":"NAPSA"},"synonyms":[{"value":"NAP1"},{"value":"NAPA"}]}],"comments":[{"texts":[{"value":"May be involved in processing of pneumocyte surfactant precursors"}],"commentType":"FUNCTION"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"O96009","intActId":"EBI-7196415"},"interactantTwo":{"uniProtKBAccession":"Q5T7P3","geneName":"LCE1B","intActId":"EBI-10245913"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"O96009","intActId":"EBI-7196415"},"interactantTwo":{"uniProtKBAccession":"Q5TA76","geneName":"LCE3A","intActId":"EBI-9394625"},"numberOfExperiments":3,"organismDiffer":false}]},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"value":"Expressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the peptidase A1 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":25,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Propeptide","location":{"start":{"value":26,"modifier":"EXACT"},"end":{"value":63,"modifier":"EXACT"}},"description":"Activation peptide","featureId":"PRO_0000025996"},{"type":"Chain","location":{"start":{"value":64,"modifier":"EXACT"},"end":{"value":420,"modifier":"EXACT"}},"description":"Napsin-A","featureId":"PRO_0000025997"},{"type":"Domain","location":{"start":{"value":78,"modifier":"EXACT"},"end":{"value":399,"modifier":"EXACT"}},"description":"Peptidase A1","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01103"}]},{"type":"Active site","location":{"start":{"value":96,"modifier":"EXACT"},"end":{"value":96,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU10094"}]},{"type":"Active site","location":{"start":{"value":283,"modifier":"EXACT"},"end":{"value":283,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU10094"}]},{"type":"Glycosylation","location":{"start":{"value":90,"modifier":"EXACT"},"end":{"value":90,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":133,"modifier":"EXACT"},"end":{"value":133,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":336,"modifier":"EXACT"},"end":{"value":336,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""},{"type":"Disulfide bond","location":{"start":{"value":109,"modifier":"EXACT"},"end":{"value":116,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Disulfide bond","location":{"start":{"value":274,"modifier":"EXACT"},"end":{"value":278,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Disulfide bond","location":{"start":{"value":317,"modifier":"EXACT"},"end":{"value":354,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Natural variant","location":{"start":{"value":40,"modifier":"EXACT"},"end":{"value":40,"modifier":"EXACT"}},"description":"in dbSNP:rs676314","featureCrossReferences":[{"database":"dbSNP","id":"rs676314"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15489334"}],"featureId":"VAR_051510","alternativeSequence":{"originalSequence":"I","alternativeSequences":["T"]}},{"type":"Natural variant","location":{"start":{"value":310,"modifier":"EXACT"},"end":{"value":310,"modifier":"EXACT"}},"description":"in dbSNP:rs11670727","featureCrossReferences":[{"database":"dbSNP","id":"rs11670727"}],"featureId":"VAR_024586","alternativeSequence":{"originalSequence":"A","alternativeSequences":["T"]}}],"keywords":[{"id":"KW-0064","category":"Molecular function","name":"Aspartyl protease"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"id":"KW-0325","category":"PTM","name":"Glycoprotein"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0645","category":"Molecular function","name":"Protease"},{"id":"KW-1267","category":"Technical term","name":"Proteomics identification"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"id":"KW-0732","category":"Domain","name":"Signal"},{"id":"KW-0865","category":"PTM","name":"Zymogen"}],"references":[{"referenceNumber":1,"citation":{"id":"9877162","citationType":"journal article","authors":["Tatnell P.J.","Powell D.J.","Hill J.","Smith T.S.","Tew D.G.","Kay J."],"citationCrossReferences":[{"database":"PubMed","id":"9877162"},{"database":"DOI","id":"10.1016/s0014-5793(98)01522-1"}],"title":"Napsins: new human aspartic proteinases. Distinction between two closely related genes.","publicationDate":"1998","journal":"FEBS Lett.","firstPage":"43","lastPage":"48","volume":"441"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]"],"referenceComments":[{"value":"Kidney","type":"TISSUE"},{"value":"Lung","type":"TISSUE"}]},{"referenceNumber":2,"citation":{"id":"10580105","citationType":"journal article","authors":["Chuman Y.","Bergman A.-C.","Ueno T.","Saito S.","Sakaguchi K.","Alaiya A.A.","Franzen B.","Bergman T.","Arnott D.","Auer G.","Appella E.","Joernvall H.","Linder S."],"citationCrossReferences":[{"database":"PubMed","id":"10580105"},{"database":"DOI","id":"10.1016/s0014-5793(99)01493-3"}],"title":"Napsin A, a member of the aspartic protease family, is abundantly expressed in normal lung and kidney tissue and is expressed in lung adenocarcinomas.","publicationDate":"1999","journal":"FEBS Lett.","firstPage":"129","lastPage":"134","volume":"462"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]","PARTIAL PROTEIN SEQUENCE","IDENTIFICATION BY MASS SPECTROMETRY"],"referenceComments":[{"value":"Fetal lung","type":"TISSUE"}]},{"referenceNumber":3,"citation":{"id":"10591213","citationType":"journal article","authors":["Yan R.","Bienkowski M.J.","Shuck M.E.","Miao H.","Tory M.C.","Pauley A.M.","Brashier J.R.","Stratman N.C.","Mathews W.R.","Buhl A.E.","Carter D.B.","Tomasselli A.G.","Parodi L.A.","Heinrikson R.L.","Gurney M.E."],"citationCrossReferences":[{"database":"PubMed","id":"10591213"},{"database":"DOI","id":"10.1038/990107"}],"title":"Membrane-anchored aspartyl protease with Alzheimer's disease beta-secretase activity.","publicationDate":"1999","journal":"Nature","firstPage":"533","lastPage":"537","volume":"402"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]"]},{"referenceNumber":4,"citation":{"id":"CI-2VA35UJLRG0H2","citationType":"submission","authors":["Koelsch G.","Wu S.","Henthorn J.","Tang J.","Lin X."],"title":"New human aspartic proteases napsin 1 and napsin 2: molecular cloning and intracellular localization of napsin 1.","publicationDate":"OCT-1998","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]"],"referenceComments":[{"value":"Liver","type":"TISSUE"}]},{"referenceNumber":5,"citation":{"id":"15489334","citationType":"journal article","authoringGroup":["The MGC Project Team"],"citationCrossReferences":[{"database":"PubMed","id":"15489334"},{"database":"DOI","id":"10.1101/gr.2596504"}],"title":"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).","publicationDate":"2004","journal":"Genome Res.","firstPage":"2121","lastPage":"2127","volume":"14"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]","VARIANT THR-40"],"referenceComments":[{"value":"Lung","type":"TISSUE"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF090386","properties":[{"key":"ProteinId","value":"AAD04917.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AF200345","properties":[{"key":"ProteinId","value":"AAF17081.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AF098484","properties":[{"key":"ProteinId","value":"AAD13215.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC017842","properties":[{"key":"ProteinId","value":"AAH17842.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"CCDS","id":"CCDS12794.1","properties":[{"key":"Description","value":"-"}]},{"database":"RefSeq","id":"NP_001423265.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001436336.1"}]},{"database":"RefSeq","id":"NP_004842.1","properties":[{"key":"NucleotideSequenceId","value":"NM_004851.3"}]},{"database":"AlphaFoldDB","id":"O96009","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"O96009","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"114861","properties":[{"key":"Interactions","value":"11"}]},{"database":"FunCoup","id":"O96009","properties":[{"key":"Number of interactors","value":"197"}]},{"database":"IntAct","id":"O96009","properties":[{"key":"Interactions","value":"10"}]},{"database":"MINT","id":"O96009","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"9606.ENSP00000253719","properties":[{"key":"Description","value":"-"}]},{"database":"MEROPS","id":"A01.046","properties":[{"key":"Description","value":"-"}]},{"database":"GlyCosmos","id":"O96009","properties":[{"key":"glycosylation","value":"3 sites, No reported glycans"}]},{"database":"GlyGen","id":"O96009","properties":[{"key":"glycosylation","value":"4 sites, 76 N-linked glycans (1 site), 1 O-linked glycan (1 site)"}]},{"database":"iPTMnet","id":"O96009","properties":[{"key":"Description","value":"-"}]},{"database":"PhosphoSitePlus","id":"O96009","properties":[{"key":"Description","value":"-"}]},{"database":"SwissPalm","id":"O96009","properties":[{"key":"Description","value":"-"}]},{"database":"BioMuta","id":"NAPSA","properties":[{"key":"Description","value":"-"}]},{"database":"MassIVE","id":"O96009","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"9606-ENSP00000253719","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"O96009","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"51189","properties":[{"key":"Description","value":"-"}]},{"database":"Antibodypedia","id":"3755","properties":[{"key":"antibodies","value":"971 antibodies from 44 providers"}]},{"database":"DNASU","id":"9476","properties":[{"key":"Description","value":"-"}]},{"database":"Ensembl","id":"ENST00000253719.7","properties":[{"key":"ProteinId","value":"ENSP00000253719.1"},{"key":"GeneId","value":"ENSG00000131400.10"}]},{"database":"GeneID","id":"9476","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"hsa:9476","properties":[{"key":"Description","value":"-"}]},{"database":"MANE-Select","id":"ENST00000253719.7","properties":[{"key":"ProteinId","value":"ENSP00000253719.1"},{"key":"RefSeqNucleotideId","value":"NM_004851.3"},{"key":"RefSeqProteinId","value":"NP_004842.1"}]},{"database":"UCSC","id":"uc002prx.4","properties":[{"key":"OrganismName","value":"human"}]},{"database":"AGR","id":"HGNC:13395","properties":[{"key":"Description","value":"-"}]},{"database":"ClinPGx","id":"PA134891814","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"9476","properties":[{"key":"Description","value":"-"}]},{"database":"DisGeNET","id":"9476","properties":[{"key":"Description","value":"-"}]},{"database":"GeneCards","id":"NAPSA","properties":[{"key":"Description","value":"-"}]},{"database":"HGNC","id":"HGNC:13395","properties":[{"key":"GeneName","value":"NAPSA"}]},{"database":"HPA","id":"ENSG00000131400","properties":[{"key":"ExpressionPatterns","value":"Tissue enriched (lung)"}]},{"database":"MIM","id":"605631","properties":[{"key":"Type","value":"gene"}]},{"database":"OpenTargets","id":"ENSG00000131400","properties":[{"key":"Description","value":"-"}]},{"database":"VEuPathDB","id":"HostDB:ENSG00000131400","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG1339","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"GeneTree","id":"ENSGT00940000160179","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"O96009","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"DYVIQIS","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"771136at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PAN-GO","id":"O96009","properties":[{"key":"Number of GO annotations","value":"4 GO annotations based on evolutionary models"}]},{"database":"PhylomeDB","id":"O96009","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"3.4.23.B1","properties":[{"key":"OrganismId","value":"2681"}]},{"database":"PathwayCommons","id":"O96009","properties":[{"key":"Description","value":"-"}]},{"database":"Reactome","id":"R-HSA-5683826","properties":[{"key":"PathwayName","value":"Surfactant metabolism"}]},{"database":"SignaLink","id":"O96009","properties":[{"key":"Description","value":"-"}]},{"database":"Agora","id":"ENSG00000131400","properties":[{"key":"NominatedTarget","value":"-"}]},{"database":"BioGRID-ORCS","id":"9476","properties":[{"key":"hits","value":"9 hits in 1156 CRISPR screens"}]},{"database":"GeneWiki","id":"NAPSA","properties":[{"key":"Description","value":"-"}]},{"database":"GenomeRNAi","id":"9476","properties":[{"key":"Description","value":"-"}]},{"database":"Pharos","id":"O96009","properties":[{"key":"DevelopmentLevel","value":"Tbio"}]},{"database":"PRO","id":"PR:O96009","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000005640","properties":[{"key":"Component","value":"Chromosome 19"}]},{"database":"RNAct","id":"O96009","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"Bgee","id":"ENSG00000131400","properties":[{"key":"ExpressionPatterns","value":"Expressed in lower lobe of lung and 112 other cell types or tissues"}]},{"database":"ExpressionAtlas","id":"O96009","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0097208","properties":[{"key":"GoTerm","value":"C:alveolar lamellar body"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"18216060"}]},{"database":"GO","id":"GO:0070062","properties":[{"key":"GoTerm","value":"C:extracellular exosome"},{"key":"GoEvidenceType","value":"HDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0007005","source":"PubMed","id":"19056867"},{"evidenceCode":"ECO:0007005","source":"PubMed","id":"23533145"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"18216060"}]},{"database":"GO","id":"GO:0005764","properties":[{"key":"GoTerm","value":"C:lysosome"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"14766755"}]},{"database":"GO","id":"GO:0097486","properties":[{"key":"GoTerm","value":"C:multivesicular body lumen"},{"key":"GoEvidenceType","value":"TAS:Reactome"}]},{"database":"GO","id":"GO:0004190","properties":[{"key":"GoTerm","value":"F:aspartic-type endopeptidase activity"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0004175","properties":[{"key":"GoTerm","value":"F:endopeptidase activity"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"14766755"}]},{"database":"GO","id":"GO:0008233","properties":[{"key":"GoTerm","value":"F:peptidase activity"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"18216060"}]},{"database":"GO","id":"GO:0033619","properties":[{"key":"GoTerm","value":"P:membrane protein proteolysis"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"14766755"}]},{"database":"GO","id":"GO:0006508","properties":[{"key":"GoTerm","value":"P:proteolysis"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0043129","properties":[{"key":"GoTerm","value":"P:surfactant homeostasis"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"18216060"}]},{"database":"FunFam","id":"2.40.70.10:FF:000048","properties":[{"key":"EntryName","value":"Napsin A aspartic peptidase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.40.70.10:FF:000066","properties":[{"key":"EntryName","value":"Napsin A aspartic peptidase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.70.10","properties":[{"key":"EntryName","value":"Acid Proteases"},{"key":"MatchStatus","value":"3"}]},{"database":"InterPro","id":"IPR001461","properties":[{"key":"EntryName","value":"Aspartic_peptidase_A1"}]},{"database":"InterPro","id":"IPR001969","properties":[{"key":"EntryName","value":"Aspartic_peptidase_AS"}]},{"database":"InterPro","id":"IPR033121","properties":[{"key":"EntryName","value":"PEPTIDASE_A1"}]},{"database":"InterPro","id":"IPR021109","properties":[{"key":"EntryName","value":"Peptidase_aspartic_dom_sf"}]},{"database":"PANTHER","id":"PTHR47966","properties":[{"key":"EntryName","value":"BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR47966:SF83","properties":[{"key":"EntryName","value":"NAPSIN-A"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00026","properties":[{"key":"EntryName","value":"Asp"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00792","properties":[{"key":"EntryName","value":"PEPSIN"}]},{"database":"SUPFAM","id":"SSF50630","properties":[{"key":"EntryName","value":"Acid proteases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00141","properties":[{"key":"EntryName","value":"ASP_PROTEASE"},{"key":"MatchStatus","value":"2"}]},{"database":"PROSITE","id":"PS51767","properties":[{"key":"EntryName","value":"PEPTIDASE_A1"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSPPPLLQPLLLLLPLLNVEPSGATLIRIPLHRVQPGRRILNLLRGWREPAELPKLGAPSPGDKPIFVPLSNYRDVQYFGEIGLGTPPQNFTVAFDTGSSNLWVPSRRCHFFSVPCWLHHRFDPKASSSFQANGTKFAIQYGTGRVDGILSEDKLTIGGIKGASVIFGEALWEPSLVFAFAHFDGILGLGFPILSVEGVRPPMDVLVEQGLLDKPVFSFYLNRDPEEPDGGELVLGGSDPAHYIPPLTFVPVTVPAYWQIHMERVKVGPGLTLCAKGCAAILDTGTSLITGPTEEIRALHAAIGGIPLLAGEYIILCSEIPKLPAVSFLLGGVWFNLTAHDYVIQTTRNGVRLCLSGFQALDVPPPAGPFWILGDVFLGTYVAVFDRGDMKSSARVGLARARTRGADLGWGETAQAQFPG","length":420,"molWeight":45387,"crc64":"018B86AE5BD0C865","md5":"AA30FA14B4A0D151AF1B9BE368F55A1A"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"INTERACTION":2,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"SIMILARITY":1},"countByFeatureType":{"Signal":1,"Propeptide":1,"Chain":1,"Domain":1,"Active site":2,"Glycosylation":3,"Disulfide bond":3,"Natural variant":2},"uniParcId":"UPI0000046F0E"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"M0QXC5","uniProtkbId":"M0QXC5_HUMAN","entryAudit":{"firstPublicDate":"2013-04-03","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2023-09-13","entryVersion":79,"sequenceVersion":2},"annotationScore":3.0,"organism":{"scientificName":"Homo sapiens","commonName":"Human","taxonId":9606,"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000469061.2"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}],"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Primates","Haplorrhini","Catarrhini","Hominidae","Homo"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}],"value":"Pepsin A"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}],"value":"3.4.23.1"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000469061.2"}],"value":"NAPSA"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00055304"}],"value":"May be involved in processing of pneumocyte surfactant precursors"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.","ecNumber":"3.4.23.1","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004613"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007447"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000454"}],"value":"Belongs to the peptidase A1 family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":24,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}]},{"type":"Chain","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":411,"modifier":"EXACT"}},"description":"Pepsin A","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}],"featureId":"PRO_5040109165"},{"type":"Domain","location":{"start":{"value":78,"modifier":"EXACT"},"end":{"value":390,"modifier":"EXACT"}},"description":"Peptidase A1","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51767"}]},{"type":"Active site","location":{"start":{"value":96,"modifier":"EXACT"},"end":{"value":96,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR601461-1"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}]},{"type":"Active site","location":{"start":{"value":274,"modifier":"EXACT"},"end":{"value":274,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR601461-1"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}]},{"type":"Disulfide bond","location":{"start":{"value":265,"modifier":"EXACT"},"end":{"value":269,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR601461-2"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022750"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"id":"KW-0064","category":"Molecular function","name":"Aspartyl protease"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023157"},{"evidenceCode":"ECO:0000256","source":"PIRSR","id":"PIRSR601461-2"}],"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023180"}],"id":"KW-0325","category":"PTM","name":"Glycoprotein"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022801"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022670"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"id":"KW-0645","category":"Molecular function","name":"Protease"},{"evidences":[{"evidenceCode":"ECO:0007829","source":"PeptideAtlas","id":"M0QXC5"},{"evidenceCode":"ECO:0007829","source":"ProteomicsDB","id":"M0QXC5"}],"id":"KW-1267","category":"Technical term","name":"Proteomics identification"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022525"}],"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022729"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}],"id":"KW-0732","category":"Domain","name":"Signal"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023145"}],"id":"KW-0865","category":"PTM","name":"Zymogen"}],"references":[{"referenceNumber":1,"citation":{"id":"11237011","citationType":"journal article","authoringGroup":["International Human Genome Sequencing Consortium"],"authors":["Lander E.S.","Linton L.M.","Birren B.","Nusbaum C.","Zody M.C.","Baldwin J.","Devon K.","Dewar K.","Doyle M.","FitzHugh W.","Funke R.","Gage D.","Harris K.","Heaford A.","Howland J.","Kann L.","Lehoczky J.","LeVine R.","McEwan P.","McKernan K.","Meldrim J.","Mesirov J.P.","Miranda C.","Morris W.","Naylor J.","Raymond C.","Rosetti M.","Santos R.","Sheridan A.","Sougnez C.","Stange-Thomann N.","Stojanovic N.","Subramanian A.","Wyman D.","Rogers J.","Sulston J.","Ainscough R.","Beck S.","Bentley D.","Burton J.","Clee C.","Carter N.","Coulson A.","Deadman R.","Deloukas P.","Dunham A.","Dunham I.","Durbin R.","French L.","Grafham D.","Gregory S.","Hubbard T.","Humphray S.","Hunt A.","Jones M.","Lloyd C.","McMurray A.","Matthews L.","Mercer S.","Milne S.","Mullikin J.C.","Mungall A.","Plumb R.","Ross M.","Shownkeen R.","Sims S.","Waterston R.H.","Wilson R.K.","Hillier L.W.","McPherson J.D.","Marra M.A.","Mardis E.R.","Fulton L.A.","Chinwalla A.T.","Pepin K.H.","Gish W.R.","Chissoe S.L.","Wendl M.C.","Delehaunty K.D.","Miner T.L.","Delehaunty A.","Kramer J.B.","Cook L.L.","Fulton R.S.","Johnson D.L.","Minx P.J.","Clifton S.W.","Hawkins T.","Branscomb E.","Predki P.","Richardson P.","Wenning S.","Slezak T.","Doggett N.","Cheng J.F.","Olsen A.","Lucas S.","Elkin C.","Uberbacher E.","Frazier M.","Gibbs R.A.","Muzny D.M.","Scherer S.E.","Bouck J.B.","Sodergren E.J.","Worley K.C.","Rives C.M.","Gorrell J.H.","Metzker M.L.","Naylor S.L.","Kucherlapati R.S.","Nelson D.L.","Weinstock G.M.","Sakaki Y.","Fujiyama A.","Hattori M.","Yada T.","Toyoda A.","Itoh T.","Kawagoe C.","Watanabe H.","Totoki Y.","Taylor T.","Weissenbach J.","Heilig R.","Saurin W.","Artiguenave F.","Brottier P.","Bruls T.","Pelletier E.","Robert C.","Wincker P.","Smith D.R.","Doucette-Stamm L.","Rubenfield M.","Weinstock K.","Lee H.M.","Dubois J.","Rosenthal A.","Platzer M.","Nyakatura G.","Taudien S.","Rump A.","Yang H.","Yu J.","Wang J.","Huang G.","Gu J.","Hood L.","Rowen L.","Madan A.","Qin S.","Davis R.W.","Federspiel N.A.","Abola A.P.","Proctor M.J.","Myers R.M.","Schmutz J.","Dickson M.","Grimwood J.","Cox D.R.","Olson M.V.","Kaul R.","Raymond C.","Shimizu N.","Kawasaki K.","Minoshima S.","Evans G.A.","Athanasiou M.","Schultz R.","Roe B.A.","Chen F.","Pan H.","Ramser J.","Lehrach H.","Reinhardt R.","McCombie W.R.","de la Bastide M.","Dedhia N.","Blocker H.","Hornischer K.","Nordsiek G.","Agarwala R.","Aravind L.","Bailey J.A.","Bateman A.","Batzoglou S.","Birney E.","Bork P.","Brown D.G.","Burge C.B.","Cerutti L.","Chen H.C.","Church D.","Clamp M.","Copley R.R.","Doerks T.","Eddy S.R.","Eichler E.E.","Furey T.S.","Galagan J.","Gilbert J.G.","Harmon C.","Hayashizaki Y.","Haussler D.","Hermjakob H.","Hokamp K.","Jang W.","Johnson L.S.","Jones T.A.","Kasif S.","Kaspryzk A.","Kennedy S.","Kent W.J.","Kitts P.","Koonin E.V.","Korf I.","Kulp D.","Lancet D.","Lowe T.M.","McLysaght A.","Mikkelsen T.","Moran J.V.","Mulder N.","Pollara V.J.","Ponting C.P.","Schuler G.","Schultz J.","Slater G.","Smit A.F.","Stupka E.","Szustakowski J.","Thierry-Mieg D.","Thierry-Mieg J.","Wagner L.","Wallis J.","Wheeler R.","Williams A.","Wolf Y.I.","Wolfe K.H.","Yang S.P.","Yeh R.F.","Collins F.","Guyer M.S.","Peterson J.","Felsenfeld A.","Wetterstrand K.A.","Patrinos A.","Morgan M.J.","de Jong P.","Catanese J.J.","Osoegawa K.","Shizuya H.","Choi S.","Chen Y.J."],"citationCrossReferences":[{"database":"PubMed","id":"11237011"},{"database":"DOI","id":"10.1038/35057062"}],"title":"Initial sequencing and analysis of the human genome.","publicationDate":"2001","journal":"Nature","firstPage":"860","lastPage":"921","volume":"409"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000469061.2"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":2,"citation":{"id":"15057824","citationType":"journal article","authors":["Grimwood J.","Gordon L.A.","Olsen A.","Terry A.","Schmutz J.","Lamerdin J.","Hellsten U.","Goodstein D.","Couronne O.","Tran-Gyamfi M.","Aerts A.","Altherr M.","Ashworth L.","Bajorek E.","Black S.","Branscomb E.","Caenepeel S.","Carrano A.","Caoile C.","Chan Y.M.","Christensen M.","Cleland C.A.","Copeland A.","Dalin E.","Dehal P.","Denys M.","Detter J.C.","Escobar J.","Flowers D.","Fotopulos D.","Garcia C.","Georgescu A.M.","Glavina T.","Gomez M.","Gonzales E.","Groza M.","Hammon N.","Hawkins T.","Haydu L.","Ho I.","Huang W.","Israni S.","Jett J.","Kadner K.","Kimball H.","Kobayashi A.","Larionov V.","Leem S.H.","Lopez F.","Lou Y.","Lowry S.","Malfatti S.","Martinez D.","McCready P.","Medina C.","Morgan J.","Nelson K.","Nolan M.","Ovcharenko I.","Pitluck S.","Pollard M.","Popkie A.P.","Predki P.","Quan G.","Ramirez L.","Rash S.","Retterer J.","Rodriguez A.","Rogers S.","Salamov A.","Salazar A.","She X.","Smith D.","Slezak T.","Solovyev V.","Thayer N.","Tice H.","Tsai M.","Ustaszewska A.","Vo N.","Wagner M.","Wheeler J.","Wu K.","Xie G.","Yang J.","Dubchak I.","Furey T.S.","DeJong P.","Dickson M.","Gordon D.","Eichler E.E.","Pennacchio L.A.","Richardson P.","Stubbs L.","Rokhsar D.S.","Myers R.M.","Rubin E.M.","Lucas S.M."],"citationCrossReferences":[{"database":"PubMed","id":"15057824"},{"database":"DOI","id":"10.1038/nature02399"}],"title":"The DNA sequence and biology of human chromosome 19.","publicationDate":"2004","journal":"Nature","firstPage":"529","lastPage":"535","volume":"428"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000469061.2"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":3,"citation":{"id":"15496913","citationType":"journal article","authoringGroup":["International Human Genome Sequencing Consortium"],"citationCrossReferences":[{"database":"PubMed","id":"15496913"},{"database":"DOI","id":"10.1038/nature03001"}],"title":"Finishing the euchromatic sequence of the human genome.","publicationDate":"2004","journal":"Nature","firstPage":"931","lastPage":"945","volume":"431"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000469061.2"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":4,"citation":{"id":"CI-20IM145UGQVF7","citationType":"submission","authoringGroup":["Ensembl"],"publicationDate":"JAN-2026","submissionDatabase":"UniProtKB"},"referencePositions":["IDENTIFICATION"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000469061.2"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AC008655","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"XP_016883001.1","properties":[{"key":"NucleotideSequenceId","value":"XM_017027512.2"}]},{"database":"RefSeq","id":"XP_054178684.1","properties":[{"key":"NucleotideSequenceId","value":"XM_054322709.1"}]},{"database":"AlphaFoldDB","id":"M0QXC5","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"M0QXC5","properties":[{"key":"Description","value":"-"}]},{"database":"MassIVE","id":"M0QXC5","properties":[{"key":"Description","value":"-"}]},{"database":"Antibodypedia","id":"3755","properties":[{"key":"antibodies","value":"971 antibodies from 44 providers"}]},{"database":"Ensembl","id":"ENST00000598915.6","properties":[{"key":"ProteinId","value":"ENSP00000469061.2"},{"key":"GeneId","value":"ENSG00000131400.10"}]},{"database":"GeneID","id":"9476","properties":[{"key":"Description","value":"-"}]},{"database":"UCSC","id":"uc061bpq.1","properties":[{"key":"OrganismName","value":"human"}]},{"database":"CTD","id":"9476","properties":[{"key":"Description","value":"-"}]},{"database":"HGNC","id":"HGNC:13395","properties":[{"key":"GeneName","value":"NAPSA"}]},{"database":"VEuPathDB","id":"HostDB:ENSG00000131400","properties":[{"key":"Description","value":"-"}]},{"database":"GeneTree","id":"ENSGT00940000160179","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_013253_3_3_1","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"771136at2759","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000005640","properties":[{"key":"Component","value":"Chromosome 19"}]},{"database":"Bgee","id":"ENSG00000131400","properties":[{"key":"ExpressionPatterns","value":"Expressed in lower lobe of lung and 112 other cell types or tissues"}]},{"database":"ExpressionAtlas","id":"M0QXC5","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0097208","properties":[{"key":"GoTerm","value":"C:alveolar lamellar body"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005764","properties":[{"key":"GoTerm","value":"C:lysosome"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0004190","properties":[{"key":"GoTerm","value":"F:aspartic-type endopeptidase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0033619","properties":[{"key":"GoTerm","value":"P:membrane protein proteolysis"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"FunFam","id":"2.40.70.10:FF:000048","properties":[{"key":"EntryName","value":"Napsin A aspartic peptidase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.40.70.10:FF:000066","properties":[{"key":"EntryName","value":"Napsin A aspartic peptidase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.70.10","properties":[{"key":"EntryName","value":"Acid Proteases"},{"key":"MatchStatus","value":"3"}]},{"database":"InterPro","id":"IPR001461","properties":[{"key":"EntryName","value":"Aspartic_peptidase_A1"}]},{"database":"InterPro","id":"IPR001969","properties":[{"key":"EntryName","value":"Aspartic_peptidase_AS"}]},{"database":"InterPro","id":"IPR033121","properties":[{"key":"EntryName","value":"PEPTIDASE_A1"}]},{"database":"InterPro","id":"IPR021109","properties":[{"key":"EntryName","value":"Peptidase_aspartic_dom_sf"}]},{"database":"PANTHER","id":"PTHR47966","properties":[{"key":"EntryName","value":"BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR47966:SF83","properties":[{"key":"EntryName","value":"NAPSIN-A"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00026","properties":[{"key":"EntryName","value":"Asp"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00792","properties":[{"key":"EntryName","value":"PEPSIN"}]},{"database":"SUPFAM","id":"SSF50630","properties":[{"key":"EntryName","value":"Acid proteases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00141","properties":[{"key":"EntryName","value":"ASP_PROTEASE"},{"key":"MatchStatus","value":"2"}]},{"database":"PROSITE","id":"PS51767","properties":[{"key":"EntryName","value":"PEPTIDASE_A1"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSPPPLLQPLLLLLPLLNVEPSGATLIRIPLHRVQPGRRILNLLRGWREPAELPKLGAPSPGDKPIFVPLSNYRDVQYFGEIGLGTPPQNFTVAFDTGSSNLWVPSRRCHFFSVPCSSSSFQANGTKFAIQYGTGRVDGILSEDKLTIGGIKGASVIFGEALWEPSLVFAFAHFDGILGLGFPILSVEGVRPPMDVLVEQGLLDKPVFSFYLNRDPEEPDGGELVLGGSDPAHYIPPLTFVPVTVPAYWQIHMERVKVGPGLTLCAKGCAAILDTGTSLITGPTEEIRALHAAIGGIPLLAGEYIILCSEIPKLPAVSFLLGGVWFNLTAHDYVIQTTRNGVRLCLSGFQALDVPPPAGPFWILGDVFLGTYVAVFDRGDMKSSARVGLARARTRGADLGWGETAQAQFPG","length":411,"molWeight":44185,"crc64":"985C538C8ABAB443","md5":"F7C7886B7B67D151DC6763639E97DED8"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Signal":1,"Chain":1,"Domain":1,"Active site":2,"Disulfide bond":1},"uniParcId":"UPI000040C436"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0ACI8PGU6","uniProtkbId":"A0ACI8PGU6_HUMAN","entryAudit":{"firstPublicDate":"2026-06-10","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2026-06-10","entryVersion":2,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Homo sapiens","commonName":"Human","taxonId":9606,"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522747.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}],"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Primates","Haplorrhini","Catarrhini","Hominidae","Homo"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}],"value":"Pepsin A"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}],"value":"3.4.23.1"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522747.1"}],"value":"NAPSA"}}],"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.","ecNumber":"3.4.23.1","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007447"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000454"}],"value":"Belongs to the peptidase A1 family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":24,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}]},{"type":"Chain","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":373,"modifier":"EXACT"}},"description":"Pepsin A","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}],"featureId":"PRO_5051401212"},{"type":"Domain","location":{"start":{"value":78,"modifier":"EXACT"},"end":{"value":373,"modifier":"EXACT"}},"description":"Peptidase A1","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51767"}]},{"type":"Active site","location":{"start":{"value":96,"modifier":"EXACT"},"end":{"value":96,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}]},{"type":"Active site","location":{"start":{"value":283,"modifier":"EXACT"},"end":{"value":283,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022750"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"id":"KW-0064","category":"Molecular function","name":"Aspartyl protease"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000454"}],"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022670"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"id":"KW-0645","category":"Molecular function","name":"Protease"},{"evidences":[{"evidenceCode":"ECO:0007829","source":"PeptideAtlas","id":"A0ACI8PGU6"},{"evidenceCode":"ECO:0007829","source":"ProteomicsDB","id":"A0ACI8PGU6"}],"id":"KW-1267","category":"Technical term","name":"Proteomics identification"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}],"id":"KW-0732","category":"Domain","name":"Signal"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023145"}],"id":"KW-0865","category":"PTM","name":"Zymogen"}],"references":[{"referenceNumber":1,"citation":{"id":"11237011","citationType":"journal article","authoringGroup":["International Human Genome Sequencing Consortium"],"authors":["Lander E.S.","Linton L.M.","Birren B.","Nusbaum C.","Zody M.C.","Baldwin J.","Devon K.","Dewar K.","Doyle M.","FitzHugh W.","Funke R.","Gage D.","Harris K.","Heaford A.","Howland J.","Kann L.","Lehoczky J.","LeVine R.","McEwan P.","McKernan K.","Meldrim J.","Mesirov J.P.","Miranda C.","Morris W.","Naylor J.","Raymond C.","Rosetti M.","Santos R.","Sheridan A.","Sougnez C.","Stange-Thomann N.","Stojanovic N.","Subramanian A.","Wyman D.","Rogers J.","Sulston J.","Ainscough R.","Beck S.","Bentley D.","Burton J.","Clee C.","Carter N.","Coulson A.","Deadman R.","Deloukas P.","Dunham A.","Dunham I.","Durbin R.","French L.","Grafham D.","Gregory S.","Hubbard T.","Humphray S.","Hunt A.","Jones M.","Lloyd C.","McMurray A.","Matthews L.","Mercer S.","Milne S.","Mullikin J.C.","Mungall A.","Plumb R.","Ross M.","Shownkeen R.","Sims S.","Waterston R.H.","Wilson R.K.","Hillier L.W.","McPherson J.D.","Marra M.A.","Mardis E.R.","Fulton L.A.","Chinwalla A.T.","Pepin K.H.","Gish W.R.","Chissoe S.L.","Wendl M.C.","Delehaunty K.D.","Miner T.L.","Delehaunty A.","Kramer J.B.","Cook L.L.","Fulton R.S.","Johnson D.L.","Minx P.J.","Clifton S.W.","Hawkins T.","Branscomb E.","Predki P.","Richardson P.","Wenning S.","Slezak T.","Doggett N.","Cheng J.F.","Olsen A.","Lucas S.","Elkin C.","Uberbacher E.","Frazier M.","Gibbs R.A.","Muzny D.M.","Scherer S.E.","Bouck J.B.","Sodergren E.J.","Worley K.C.","Rives C.M.","Gorrell J.H.","Metzker M.L.","Naylor S.L.","Kucherlapati R.S.","Nelson D.L.","Weinstock G.M.","Sakaki Y.","Fujiyama A.","Hattori M.","Yada T.","Toyoda A.","Itoh T.","Kawagoe C.","Watanabe H.","Totoki Y.","Taylor T.","Weissenbach J.","Heilig R.","Saurin W.","Artiguenave F.","Brottier P.","Bruls T.","Pelletier E.","Robert C.","Wincker P.","Smith D.R.","Doucette-Stamm L.","Rubenfield M.","Weinstock K.","Lee H.M.","Dubois J.","Rosenthal A.","Platzer M.","Nyakatura G.","Taudien S.","Rump A.","Yang H.","Yu J.","Wang J.","Huang G.","Gu J.","Hood L.","Rowen L.","Madan A.","Qin S.","Davis R.W.","Federspiel N.A.","Abola A.P.","Proctor M.J.","Myers R.M.","Schmutz J.","Dickson M.","Grimwood J.","Cox D.R.","Olson M.V.","Kaul R.","Raymond C.","Shimizu N.","Kawasaki K.","Minoshima S.","Evans G.A.","Athanasiou M.","Schultz R.","Roe B.A.","Chen F.","Pan H.","Ramser J.","Lehrach H.","Reinhardt R.","McCombie W.R.","de la Bastide M.","Dedhia N.","Blocker H.","Hornischer K.","Nordsiek G.","Agarwala R.","Aravind L.","Bailey J.A.","Bateman A.","Batzoglou S.","Birney E.","Bork P.","Brown D.G.","Burge C.B.","Cerutti L.","Chen H.C.","Church D.","Clamp M.","Copley R.R.","Doerks T.","Eddy S.R.","Eichler E.E.","Furey T.S.","Galagan J.","Gilbert J.G.","Harmon C.","Hayashizaki Y.","Haussler D.","Hermjakob H.","Hokamp K.","Jang W.","Johnson L.S.","Jones T.A.","Kasif S.","Kaspryzk A.","Kennedy S.","Kent W.J.","Kitts P.","Koonin E.V.","Korf I.","Kulp D.","Lancet D.","Lowe T.M.","McLysaght A.","Mikkelsen T.","Moran J.V.","Mulder N.","Pollara V.J.","Ponting C.P.","Schuler G.","Schultz J.","Slater G.","Smit A.F.","Stupka E.","Szustakowski J.","Thierry-Mieg D.","Thierry-Mieg J.","Wagner L.","Wallis J.","Wheeler R.","Williams A.","Wolf Y.I.","Wolfe K.H.","Yang S.P.","Yeh R.F.","Collins F.","Guyer M.S.","Peterson J.","Felsenfeld A.","Wetterstrand K.A.","Patrinos A.","Morgan M.J.","de Jong P.","Catanese J.J.","Osoegawa K.","Shizuya H.","Choi S.","Chen Y.J."],"citationCrossReferences":[{"database":"PubMed","id":"11237011"},{"database":"DOI","id":"10.1038/35057062"}],"title":"Initial sequencing and analysis of the human genome.","publicationDate":"2001","journal":"Nature","firstPage":"860","lastPage":"921","volume":"409"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522747.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":2,"citation":{"id":"15057824","citationType":"journal article","authors":["Grimwood J.","Gordon L.A.","Olsen A.","Terry A.","Schmutz J.","Lamerdin J.","Hellsten U.","Goodstein D.","Couronne O.","Tran-Gyamfi M.","Aerts A.","Altherr M.","Ashworth L.","Bajorek E.","Black S.","Branscomb E.","Caenepeel S.","Carrano A.","Caoile C.","Chan Y.M.","Christensen M.","Cleland C.A.","Copeland A.","Dalin E.","Dehal P.","Denys M.","Detter J.C.","Escobar J.","Flowers D.","Fotopulos D.","Garcia C.","Georgescu A.M.","Glavina T.","Gomez M.","Gonzales E.","Groza M.","Hammon N.","Hawkins T.","Haydu L.","Ho I.","Huang W.","Israni S.","Jett J.","Kadner K.","Kimball H.","Kobayashi A.","Larionov V.","Leem S.H.","Lopez F.","Lou Y.","Lowry S.","Malfatti S.","Martinez D.","McCready P.","Medina C.","Morgan J.","Nelson K.","Nolan M.","Ovcharenko I.","Pitluck S.","Pollard M.","Popkie A.P.","Predki P.","Quan G.","Ramirez L.","Rash S.","Retterer J.","Rodriguez A.","Rogers S.","Salamov A.","Salazar A.","She X.","Smith D.","Slezak T.","Solovyev V.","Thayer N.","Tice H.","Tsai M.","Ustaszewska A.","Vo N.","Wagner M.","Wheeler J.","Wu K.","Xie G.","Yang J.","Dubchak I.","Furey T.S.","DeJong P.","Dickson M.","Gordon D.","Eichler E.E.","Pennacchio L.A.","Richardson P.","Stubbs L.","Rokhsar D.S.","Myers R.M.","Rubin E.M.","Lucas S.M."],"citationCrossReferences":[{"database":"PubMed","id":"15057824"},{"database":"DOI","id":"10.1038/nature02399"}],"title":"The DNA sequence and biology of human chromosome 19.","publicationDate":"2004","journal":"Nature","firstPage":"529","lastPage":"535","volume":"428"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522747.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":3,"citation":{"id":"15496913","citationType":"journal article","authoringGroup":["International Human Genome Sequencing Consortium"],"citationCrossReferences":[{"database":"PubMed","id":"15496913"},{"database":"DOI","id":"10.1038/nature03001"}],"title":"Finishing the euchromatic sequence of the human genome.","publicationDate":"2004","journal":"Nature","firstPage":"931","lastPage":"945","volume":"431"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522747.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":4,"citation":{"id":"CI-20IM145UGQVF7","citationType":"submission","authoringGroup":["Ensembl"],"publicationDate":"JAN-2026","submissionDatabase":"UniProtKB"},"referencePositions":["IDENTIFICATION"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522747.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AC008655","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"Ensembl","id":"ENST00000852688.1","properties":[{"key":"ProteinId","value":"ENSP00000522747.1"},{"key":"GeneId","value":"ENSG00000131400.10"}]},{"database":"HGNC","id":"HGNC:13395","properties":[{"key":"GeneName","value":"NAPSA"}]},{"database":"OpenTargets","id":"ENSG00000131400","properties":[{"key":"Description","value":"-"}]},{"database":"GeneTree","id":"ENSGT00940000160179","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000005640","properties":[{"key":"Component","value":"Chromosome 19"}]},{"database":"FunFam","id":"2.40.70.10:FF:000048","properties":[{"key":"EntryName","value":"Napsin A aspartic peptidase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.40.70.10:FF:000059","properties":[{"key":"EntryName","value":"Napsin A aspartic peptidase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.70.10","properties":[{"key":"EntryName","value":"Acid Proteases"},{"key":"MatchStatus","value":"3"}]},{"database":"InterPro","id":"IPR001461","properties":[{"key":"EntryName","value":"Aspartic_peptidase_A1"}]},{"database":"InterPro","id":"IPR001969","properties":[{"key":"EntryName","value":"Aspartic_peptidase_AS"}]},{"database":"InterPro","id":"IPR033121","properties":[{"key":"EntryName","value":"PEPTIDASE_A1"}]},{"database":"InterPro","id":"IPR021109","properties":[{"key":"EntryName","value":"Peptidase_aspartic_dom_sf"}]},{"database":"PANTHER","id":"PTHR47966","properties":[{"key":"EntryName","value":"BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR47966:SF83","properties":[{"key":"EntryName","value":"NAPSIN-A"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00026","properties":[{"key":"EntryName","value":"Asp"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00792","properties":[{"key":"EntryName","value":"PEPSIN"}]},{"database":"SUPFAM","id":"SSF50630","properties":[{"key":"EntryName","value":"Acid proteases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00141","properties":[{"key":"EntryName","value":"ASP_PROTEASE"},{"key":"MatchStatus","value":"2"}]},{"database":"PROSITE","id":"PS51767","properties":[{"key":"EntryName","value":"PEPTIDASE_A1"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSPPPLLQPLLLLLPLLNVEPSGATLIRIPLHRVQPGRRILNLLRGWREPAELPKLGAPSPGDKPIFVPLSNYRDVQYFGEIGLGTPPQNFTVAFDTGSSNLWVPSRRCHFFSVPCWLHHRFDPKASSSFQANGTKFAIQYGTGRVDGILSEDKLTIGGIKGASVIFGEALWEPSLVFAFAHFDGILGLGFPILSVEGVRPPMDVLVEQGLLDKPVFSFYLNRDPEEPDGGELVLGGSDPAHYIPPLTFVPVTVPAYWQIHMERVKVGPGLTLCAKGCAAILDTGTSLITGPTEEIRALHAAIGGIPLLAGEALDVPPPAGPFWILGDVFLGTYVAVFDRGDMKSSARVGLARARTRGADLGWGETAQAQFPG","length":373,"molWeight":40149,"crc64":"610137671A394D9D","md5":"C650AC572F7DA7B626B4C10E8BA0E6E6"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Signal":1,"Chain":1,"Domain":1,"Active site":2},"uniParcId":"UPI0040319063"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0ACI8Q363","uniProtkbId":"A0ACI8Q363_HUMAN","entryAudit":{"firstPublicDate":"2026-06-10","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2026-06-10","entryVersion":2,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Homo sapiens","commonName":"Human","taxonId":9606,"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522748.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}],"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Primates","Haplorrhini","Catarrhini","Hominidae","Homo"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}],"value":"Pepsin A"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}],"value":"3.4.23.1"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522748.1"}],"value":"NAPSA"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00055304"}],"value":"May be involved in processing of pneumocyte surfactant precursors"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.","ecNumber":"3.4.23.1","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004613"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007447"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000454"}],"value":"Belongs to the peptidase A1 family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":24,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}]},{"type":"Chain","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":424,"modifier":"EXACT"}},"description":"Pepsin A","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}],"featureId":"PRO_5051371037"},{"type":"Domain","location":{"start":{"value":78,"modifier":"EXACT"},"end":{"value":403,"modifier":"EXACT"}},"description":"Peptidase A1","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51767"}]},{"type":"Active site","location":{"start":{"value":96,"modifier":"EXACT"},"end":{"value":96,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}]},{"type":"Active site","location":{"start":{"value":287,"modifier":"EXACT"},"end":{"value":287,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022750"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"id":"KW-0064","category":"Molecular function","name":"Aspartyl protease"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023157"}],"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023180"}],"id":"KW-0325","category":"PTM","name":"Glycoprotein"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022801"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022670"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"id":"KW-0645","category":"Molecular function","name":"Protease"},{"evidences":[{"evidenceCode":"ECO:0007829","source":"PeptideAtlas","id":"A0ACI8Q363"},{"evidenceCode":"ECO:0007829","source":"ProteomicsDB","id":"A0ACI8Q363"}],"id":"KW-1267","category":"Technical term","name":"Proteomics identification"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022525"}],"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022729"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}],"id":"KW-0732","category":"Domain","name":"Signal"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023145"}],"id":"KW-0865","category":"PTM","name":"Zymogen"}],"references":[{"referenceNumber":1,"citation":{"id":"11237011","citationType":"journal article","authoringGroup":["International Human Genome Sequencing Consortium"],"authors":["Lander E.S.","Linton L.M.","Birren B.","Nusbaum C.","Zody M.C.","Baldwin J.","Devon K.","Dewar K.","Doyle M.","FitzHugh W.","Funke R.","Gage D.","Harris K.","Heaford A.","Howland J.","Kann L.","Lehoczky J.","LeVine R.","McEwan P.","McKernan K.","Meldrim J.","Mesirov J.P.","Miranda C.","Morris W.","Naylor J.","Raymond C.","Rosetti M.","Santos R.","Sheridan A.","Sougnez C.","Stange-Thomann N.","Stojanovic N.","Subramanian A.","Wyman D.","Rogers J.","Sulston J.","Ainscough R.","Beck S.","Bentley D.","Burton J.","Clee C.","Carter N.","Coulson A.","Deadman R.","Deloukas P.","Dunham A.","Dunham I.","Durbin R.","French L.","Grafham D.","Gregory S.","Hubbard T.","Humphray S.","Hunt A.","Jones M.","Lloyd C.","McMurray A.","Matthews L.","Mercer S.","Milne S.","Mullikin J.C.","Mungall A.","Plumb R.","Ross M.","Shownkeen R.","Sims S.","Waterston R.H.","Wilson R.K.","Hillier L.W.","McPherson J.D.","Marra M.A.","Mardis E.R.","Fulton L.A.","Chinwalla A.T.","Pepin K.H.","Gish W.R.","Chissoe S.L.","Wendl M.C.","Delehaunty K.D.","Miner T.L.","Delehaunty A.","Kramer J.B.","Cook L.L.","Fulton R.S.","Johnson D.L.","Minx P.J.","Clifton S.W.","Hawkins T.","Branscomb E.","Predki P.","Richardson P.","Wenning S.","Slezak T.","Doggett N.","Cheng J.F.","Olsen A.","Lucas S.","Elkin C.","Uberbacher E.","Frazier M.","Gibbs R.A.","Muzny D.M.","Scherer S.E.","Bouck J.B.","Sodergren E.J.","Worley K.C.","Rives C.M.","Gorrell J.H.","Metzker M.L.","Naylor S.L.","Kucherlapati R.S.","Nelson D.L.","Weinstock G.M.","Sakaki Y.","Fujiyama A.","Hattori M.","Yada T.","Toyoda A.","Itoh T.","Kawagoe C.","Watanabe H.","Totoki Y.","Taylor T.","Weissenbach J.","Heilig R.","Saurin W.","Artiguenave F.","Brottier P.","Bruls T.","Pelletier E.","Robert C.","Wincker P.","Smith D.R.","Doucette-Stamm L.","Rubenfield M.","Weinstock K.","Lee H.M.","Dubois J.","Rosenthal A.","Platzer M.","Nyakatura G.","Taudien S.","Rump A.","Yang H.","Yu J.","Wang J.","Huang G.","Gu J.","Hood L.","Rowen L.","Madan A.","Qin S.","Davis R.W.","Federspiel N.A.","Abola A.P.","Proctor M.J.","Myers R.M.","Schmutz J.","Dickson M.","Grimwood J.","Cox D.R.","Olson M.V.","Kaul R.","Raymond C.","Shimizu N.","Kawasaki K.","Minoshima S.","Evans G.A.","Athanasiou M.","Schultz R.","Roe B.A.","Chen F.","Pan H.","Ramser J.","Lehrach H.","Reinhardt R.","McCombie W.R.","de la Bastide M.","Dedhia N.","Blocker H.","Hornischer K.","Nordsiek G.","Agarwala R.","Aravind L.","Bailey J.A.","Bateman A.","Batzoglou S.","Birney E.","Bork P.","Brown D.G.","Burge C.B.","Cerutti L.","Chen H.C.","Church D.","Clamp M.","Copley R.R.","Doerks T.","Eddy S.R.","Eichler E.E.","Furey T.S.","Galagan J.","Gilbert J.G.","Harmon C.","Hayashizaki Y.","Haussler D.","Hermjakob H.","Hokamp K.","Jang W.","Johnson L.S.","Jones T.A.","Kasif S.","Kaspryzk A.","Kennedy S.","Kent W.J.","Kitts P.","Koonin E.V.","Korf I.","Kulp D.","Lancet D.","Lowe T.M.","McLysaght A.","Mikkelsen T.","Moran J.V.","Mulder N.","Pollara V.J.","Ponting C.P.","Schuler G.","Schultz J.","Slater G.","Smit A.F.","Stupka E.","Szustakowski J.","Thierry-Mieg D.","Thierry-Mieg J.","Wagner L.","Wallis J.","Wheeler R.","Williams A.","Wolf Y.I.","Wolfe K.H.","Yang S.P.","Yeh R.F.","Collins F.","Guyer M.S.","Peterson J.","Felsenfeld A.","Wetterstrand K.A.","Patrinos A.","Morgan M.J.","de Jong P.","Catanese J.J.","Osoegawa K.","Shizuya H.","Choi S.","Chen Y.J."],"citationCrossReferences":[{"database":"PubMed","id":"11237011"},{"database":"DOI","id":"10.1038/35057062"}],"title":"Initial sequencing and analysis of the human genome.","publicationDate":"2001","journal":"Nature","firstPage":"860","lastPage":"921","volume":"409"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522748.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":2,"citation":{"id":"15057824","citationType":"journal article","authors":["Grimwood J.","Gordon L.A.","Olsen A.","Terry A.","Schmutz J.","Lamerdin J.","Hellsten U.","Goodstein D.","Couronne O.","Tran-Gyamfi M.","Aerts A.","Altherr M.","Ashworth L.","Bajorek E.","Black S.","Branscomb E.","Caenepeel S.","Carrano A.","Caoile C.","Chan Y.M.","Christensen M.","Cleland C.A.","Copeland A.","Dalin E.","Dehal P.","Denys M.","Detter J.C.","Escobar J.","Flowers D.","Fotopulos D.","Garcia C.","Georgescu A.M.","Glavina T.","Gomez M.","Gonzales E.","Groza M.","Hammon N.","Hawkins T.","Haydu L.","Ho I.","Huang W.","Israni S.","Jett J.","Kadner K.","Kimball H.","Kobayashi A.","Larionov V.","Leem S.H.","Lopez F.","Lou Y.","Lowry S.","Malfatti S.","Martinez D.","McCready P.","Medina C.","Morgan J.","Nelson K.","Nolan M.","Ovcharenko I.","Pitluck S.","Pollard M.","Popkie A.P.","Predki P.","Quan G.","Ramirez L.","Rash S.","Retterer J.","Rodriguez A.","Rogers S.","Salamov A.","Salazar A.","She X.","Smith D.","Slezak T.","Solovyev V.","Thayer N.","Tice H.","Tsai M.","Ustaszewska A.","Vo N.","Wagner M.","Wheeler J.","Wu K.","Xie G.","Yang J.","Dubchak I.","Furey T.S.","DeJong P.","Dickson M.","Gordon D.","Eichler E.E.","Pennacchio L.A.","Richardson P.","Stubbs L.","Rokhsar D.S.","Myers R.M.","Rubin E.M.","Lucas S.M."],"citationCrossReferences":[{"database":"PubMed","id":"15057824"},{"database":"DOI","id":"10.1038/nature02399"}],"title":"The DNA sequence and biology of human chromosome 19.","publicationDate":"2004","journal":"Nature","firstPage":"529","lastPage":"535","volume":"428"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522748.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":3,"citation":{"id":"15496913","citationType":"journal article","authoringGroup":["International Human Genome Sequencing Consortium"],"citationCrossReferences":[{"database":"PubMed","id":"15496913"},{"database":"DOI","id":"10.1038/nature03001"}],"title":"Finishing the euchromatic sequence of the human genome.","publicationDate":"2004","journal":"Nature","firstPage":"931","lastPage":"945","volume":"431"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522748.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":4,"citation":{"id":"CI-20IM145UGQVF7","citationType":"submission","authoringGroup":["Ensembl"],"publicationDate":"JAN-2026","submissionDatabase":"UniProtKB"},"referencePositions":["IDENTIFICATION"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522748.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AC008655","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"Ensembl","id":"ENST00000852689.1","properties":[{"key":"ProteinId","value":"ENSP00000522748.1"},{"key":"GeneId","value":"ENSG00000131400.10"}]},{"database":"HGNC","id":"HGNC:13395","properties":[{"key":"GeneName","value":"NAPSA"}]},{"database":"OpenTargets","id":"ENSG00000131400","properties":[{"key":"Description","value":"-"}]},{"database":"GeneTree","id":"ENSGT00940000160179","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000005640","properties":[{"key":"Component","value":"Chromosome 19"}]},{"database":"FunFam","id":"2.40.70.10:FF:000048","properties":[{"key":"EntryName","value":"Napsin A aspartic peptidase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.40.70.10:FF:000066","properties":[{"key":"EntryName","value":"Napsin A aspartic peptidase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.70.10","properties":[{"key":"EntryName","value":"Acid Proteases"},{"key":"MatchStatus","value":"2"}]},{"database":"InterPro","id":"IPR001461","properties":[{"key":"EntryName","value":"Aspartic_peptidase_A1"}]},{"database":"InterPro","id":"IPR001969","properties":[{"key":"EntryName","value":"Aspartic_peptidase_AS"}]},{"database":"InterPro","id":"IPR033121","properties":[{"key":"EntryName","value":"PEPTIDASE_A1"}]},{"database":"InterPro","id":"IPR021109","properties":[{"key":"EntryName","value":"Peptidase_aspartic_dom_sf"}]},{"database":"PANTHER","id":"PTHR47966","properties":[{"key":"EntryName","value":"BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR47966:SF83","properties":[{"key":"EntryName","value":"NAPSIN-A"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00026","properties":[{"key":"EntryName","value":"Asp"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00792","properties":[{"key":"EntryName","value":"PEPSIN"}]},{"database":"SUPFAM","id":"SSF50630","properties":[{"key":"EntryName","value":"Acid proteases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00141","properties":[{"key":"EntryName","value":"ASP_PROTEASE"},{"key":"MatchStatus","value":"2"}]},{"database":"PROSITE","id":"PS51767","properties":[{"key":"EntryName","value":"PEPTIDASE_A1"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSPPPLLQPLLLLLPLLNVEPSGATLIRIPLHRVQPGRRILNLLRGWREPAELPKLGAPSPGDKPIFVPLSNYRDVQYFGEIGLGTPPQNFTVAFDTGSSNLWVPSRRCHFFSVPCCELLWLHHRFDPKASSSFQANGTKFAIQYGTGRVDGILSEDKLTIGGIKGASVIFGEALWEPSLVFAFAHFDGILGLGFPILSVEGVRPPMDVLVEQGLLDKPVFSFYLNRDPEEPDGGELVLGGSDPAHYIPPLTFVPVTVPAYWQIHMERVKVGPGLTLCAKGCAAILDTGTSLITGPTEEIRALHAAIGGIPLLAGEYIILCSEIPKLPAVSFLLGGVWFNLTAHDYVIQTTRNGVRLCLSGFQALDVPPPAGPFWILGDVFLGTYVAVFDRGDMKSSARVGLARARTRGADLGWGETAQAQFPG","length":424,"molWeight":45845,"crc64":"C37C4248A83B9A08","md5":"83FA74C2E82867F13BF57B9876A143D0"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Signal":1,"Chain":1,"Domain":1,"Active site":2},"uniParcId":"UPI004031EFE8"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0ACI8R6P6","uniProtkbId":"A0ACI8R6P6_HUMAN","entryAudit":{"firstPublicDate":"2026-06-10","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2026-06-10","entryVersion":2,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Homo sapiens","commonName":"Human","taxonId":9606,"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522745.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}],"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Primates","Haplorrhini","Catarrhini","Hominidae","Homo"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}],"value":"Pepsin A"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}],"value":"3.4.23.1"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522745.1"}],"value":"NAPSA"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00055304"}],"value":"May be involved in processing of pneumocyte surfactant precursors"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.","ecNumber":"3.4.23.1","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004613"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007447"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000454"}],"value":"Belongs to the peptidase A1 family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":24,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}]},{"type":"Chain","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":406,"modifier":"EXACT"}},"description":"Pepsin A","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}],"featureId":"PRO_5051463713"},{"type":"Domain","location":{"start":{"value":78,"modifier":"EXACT"},"end":{"value":385,"modifier":"EXACT"}},"description":"Peptidase A1","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51767"}]},{"type":"Active site","location":{"start":{"value":96,"modifier":"EXACT"},"end":{"value":96,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}]},{"type":"Active site","location":{"start":{"value":283,"modifier":"EXACT"},"end":{"value":283,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022750"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"id":"KW-0064","category":"Molecular function","name":"Aspartyl protease"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023157"}],"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023180"}],"id":"KW-0325","category":"PTM","name":"Glycoprotein"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022801"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022670"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"id":"KW-0645","category":"Molecular function","name":"Protease"},{"evidences":[{"evidenceCode":"ECO:0007829","source":"PeptideAtlas","id":"A0ACI8R6P6"},{"evidenceCode":"ECO:0007829","source":"ProteomicsDB","id":"A0ACI8R6P6"}],"id":"KW-1267","category":"Technical term","name":"Proteomics identification"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022525"}],"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022729"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}],"id":"KW-0732","category":"Domain","name":"Signal"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023145"}],"id":"KW-0865","category":"PTM","name":"Zymogen"}],"references":[{"referenceNumber":1,"citation":{"id":"11237011","citationType":"journal article","authoringGroup":["International Human Genome Sequencing Consortium"],"authors":["Lander E.S.","Linton L.M.","Birren B.","Nusbaum C.","Zody M.C.","Baldwin J.","Devon K.","Dewar K.","Doyle M.","FitzHugh W.","Funke R.","Gage D.","Harris K.","Heaford A.","Howland J.","Kann L.","Lehoczky J.","LeVine R.","McEwan P.","McKernan K.","Meldrim J.","Mesirov J.P.","Miranda C.","Morris W.","Naylor J.","Raymond C.","Rosetti M.","Santos R.","Sheridan A.","Sougnez C.","Stange-Thomann N.","Stojanovic N.","Subramanian A.","Wyman D.","Rogers J.","Sulston J.","Ainscough R.","Beck S.","Bentley D.","Burton J.","Clee C.","Carter N.","Coulson A.","Deadman R.","Deloukas P.","Dunham A.","Dunham I.","Durbin R.","French L.","Grafham D.","Gregory S.","Hubbard T.","Humphray S.","Hunt A.","Jones M.","Lloyd C.","McMurray A.","Matthews L.","Mercer S.","Milne S.","Mullikin J.C.","Mungall A.","Plumb R.","Ross M.","Shownkeen R.","Sims S.","Waterston R.H.","Wilson R.K.","Hillier L.W.","McPherson J.D.","Marra M.A.","Mardis E.R.","Fulton L.A.","Chinwalla A.T.","Pepin K.H.","Gish W.R.","Chissoe S.L.","Wendl M.C.","Delehaunty K.D.","Miner T.L.","Delehaunty A.","Kramer J.B.","Cook L.L.","Fulton R.S.","Johnson D.L.","Minx P.J.","Clifton S.W.","Hawkins T.","Branscomb E.","Predki P.","Richardson P.","Wenning S.","Slezak T.","Doggett N.","Cheng J.F.","Olsen A.","Lucas S.","Elkin C.","Uberbacher E.","Frazier M.","Gibbs R.A.","Muzny D.M.","Scherer S.E.","Bouck J.B.","Sodergren E.J.","Worley K.C.","Rives C.M.","Gorrell J.H.","Metzker M.L.","Naylor S.L.","Kucherlapati R.S.","Nelson D.L.","Weinstock G.M.","Sakaki Y.","Fujiyama A.","Hattori M.","Yada T.","Toyoda A.","Itoh T.","Kawagoe C.","Watanabe H.","Totoki Y.","Taylor T.","Weissenbach J.","Heilig R.","Saurin W.","Artiguenave F.","Brottier P.","Bruls T.","Pelletier E.","Robert C.","Wincker P.","Smith D.R.","Doucette-Stamm L.","Rubenfield M.","Weinstock K.","Lee H.M.","Dubois J.","Rosenthal A.","Platzer M.","Nyakatura G.","Taudien S.","Rump A.","Yang H.","Yu J.","Wang J.","Huang G.","Gu J.","Hood L.","Rowen L.","Madan A.","Qin S.","Davis R.W.","Federspiel N.A.","Abola A.P.","Proctor M.J.","Myers R.M.","Schmutz J.","Dickson M.","Grimwood J.","Cox D.R.","Olson M.V.","Kaul R.","Raymond C.","Shimizu N.","Kawasaki K.","Minoshima S.","Evans G.A.","Athanasiou M.","Schultz R.","Roe B.A.","Chen F.","Pan H.","Ramser J.","Lehrach H.","Reinhardt R.","McCombie W.R.","de la Bastide M.","Dedhia N.","Blocker H.","Hornischer K.","Nordsiek G.","Agarwala R.","Aravind L.","Bailey J.A.","Bateman A.","Batzoglou S.","Birney E.","Bork P.","Brown D.G.","Burge C.B.","Cerutti L.","Chen H.C.","Church D.","Clamp M.","Copley R.R.","Doerks T.","Eddy S.R.","Eichler E.E.","Furey T.S.","Galagan J.","Gilbert J.G.","Harmon C.","Hayashizaki Y.","Haussler D.","Hermjakob H.","Hokamp K.","Jang W.","Johnson L.S.","Jones T.A.","Kasif S.","Kaspryzk A.","Kennedy S.","Kent W.J.","Kitts P.","Koonin E.V.","Korf I.","Kulp D.","Lancet D.","Lowe T.M.","McLysaght A.","Mikkelsen T.","Moran J.V.","Mulder N.","Pollara V.J.","Ponting C.P.","Schuler G.","Schultz J.","Slater G.","Smit A.F.","Stupka E.","Szustakowski J.","Thierry-Mieg D.","Thierry-Mieg J.","Wagner L.","Wallis J.","Wheeler R.","Williams A.","Wolf Y.I.","Wolfe K.H.","Yang S.P.","Yeh R.F.","Collins F.","Guyer M.S.","Peterson J.","Felsenfeld A.","Wetterstrand K.A.","Patrinos A.","Morgan M.J.","de Jong P.","Catanese J.J.","Osoegawa K.","Shizuya H.","Choi S.","Chen Y.J."],"citationCrossReferences":[{"database":"PubMed","id":"11237011"},{"database":"DOI","id":"10.1038/35057062"}],"title":"Initial sequencing and analysis of the human genome.","publicationDate":"2001","journal":"Nature","firstPage":"860","lastPage":"921","volume":"409"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522745.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":2,"citation":{"id":"15057824","citationType":"journal article","authors":["Grimwood J.","Gordon L.A.","Olsen A.","Terry A.","Schmutz J.","Lamerdin J.","Hellsten U.","Goodstein D.","Couronne O.","Tran-Gyamfi M.","Aerts A.","Altherr M.","Ashworth L.","Bajorek E.","Black S.","Branscomb E.","Caenepeel S.","Carrano A.","Caoile C.","Chan Y.M.","Christensen M.","Cleland C.A.","Copeland A.","Dalin E.","Dehal P.","Denys M.","Detter J.C.","Escobar J.","Flowers D.","Fotopulos D.","Garcia C.","Georgescu A.M.","Glavina T.","Gomez M.","Gonzales E.","Groza M.","Hammon N.","Hawkins T.","Haydu L.","Ho I.","Huang W.","Israni S.","Jett J.","Kadner K.","Kimball H.","Kobayashi A.","Larionov V.","Leem S.H.","Lopez F.","Lou Y.","Lowry S.","Malfatti S.","Martinez D.","McCready P.","Medina C.","Morgan J.","Nelson K.","Nolan M.","Ovcharenko I.","Pitluck S.","Pollard M.","Popkie A.P.","Predki P.","Quan G.","Ramirez L.","Rash S.","Retterer J.","Rodriguez A.","Rogers S.","Salamov A.","Salazar A.","She X.","Smith D.","Slezak T.","Solovyev V.","Thayer N.","Tice H.","Tsai M.","Ustaszewska A.","Vo N.","Wagner M.","Wheeler J.","Wu K.","Xie G.","Yang J.","Dubchak I.","Furey T.S.","DeJong P.","Dickson M.","Gordon D.","Eichler E.E.","Pennacchio L.A.","Richardson P.","Stubbs L.","Rokhsar D.S.","Myers R.M.","Rubin E.M.","Lucas S.M."],"citationCrossReferences":[{"database":"PubMed","id":"15057824"},{"database":"DOI","id":"10.1038/nature02399"}],"title":"The DNA sequence and biology of human chromosome 19.","publicationDate":"2004","journal":"Nature","firstPage":"529","lastPage":"535","volume":"428"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522745.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":3,"citation":{"id":"15496913","citationType":"journal article","authoringGroup":["International Human Genome Sequencing Consortium"],"citationCrossReferences":[{"database":"PubMed","id":"15496913"},{"database":"DOI","id":"10.1038/nature03001"}],"title":"Finishing the euchromatic sequence of the human genome.","publicationDate":"2004","journal":"Nature","firstPage":"931","lastPage":"945","volume":"431"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522745.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":4,"citation":{"id":"CI-20IM145UGQVF7","citationType":"submission","authoringGroup":["Ensembl"],"publicationDate":"JAN-2026","submissionDatabase":"UniProtKB"},"referencePositions":["IDENTIFICATION"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522745.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AC008655","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"Ensembl","id":"ENST00000852686.1","properties":[{"key":"ProteinId","value":"ENSP00000522745.1"},{"key":"GeneId","value":"ENSG00000131400.10"}]},{"database":"HGNC","id":"HGNC:13395","properties":[{"key":"GeneName","value":"NAPSA"}]},{"database":"OpenTargets","id":"ENSG00000131400","properties":[{"key":"Description","value":"-"}]},{"database":"GeneTree","id":"ENSGT00940000160179","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000005640","properties":[{"key":"Component","value":"Chromosome 19"}]},{"database":"FunFam","id":"2.40.70.10:FF:000048","properties":[{"key":"EntryName","value":"Napsin A aspartic peptidase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.40.70.10:FF:000066","properties":[{"key":"EntryName","value":"Napsin A aspartic peptidase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.70.10","properties":[{"key":"EntryName","value":"Acid Proteases"},{"key":"MatchStatus","value":"2"}]},{"database":"InterPro","id":"IPR001461","properties":[{"key":"EntryName","value":"Aspartic_peptidase_A1"}]},{"database":"InterPro","id":"IPR001969","properties":[{"key":"EntryName","value":"Aspartic_peptidase_AS"}]},{"database":"InterPro","id":"IPR033121","properties":[{"key":"EntryName","value":"PEPTIDASE_A1"}]},{"database":"InterPro","id":"IPR021109","properties":[{"key":"EntryName","value":"Peptidase_aspartic_dom_sf"}]},{"database":"PANTHER","id":"PTHR47966","properties":[{"key":"EntryName","value":"BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR47966:SF83","properties":[{"key":"EntryName","value":"NAPSIN-A"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00026","properties":[{"key":"EntryName","value":"Asp"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00792","properties":[{"key":"EntryName","value":"PEPSIN"}]},{"database":"SUPFAM","id":"SSF50630","properties":[{"key":"EntryName","value":"Acid proteases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00141","properties":[{"key":"EntryName","value":"ASP_PROTEASE"},{"key":"MatchStatus","value":"2"}]},{"database":"PROSITE","id":"PS51767","properties":[{"key":"EntryName","value":"PEPTIDASE_A1"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSPPPLLQPLLLLLPLLNVEPSGATLIRIPLHRVQPGRRILNLLRGWREPAELPKLGAPSPGDKPIFVPLSNYRDVQYFGEIGLGTPPQNFTVAFDTGSSNLWVPSRRCHFFSVPCWLHHRFDPKASSSFQANGTKFAIQYGTGRVDGILSEDKLTIGGIKGASVIFGEALWEPSLVFAFAHFDGILGLGFPILSVEGVRPPMDVLVEQGLLDKPVFSFYLNRDPEEPDGGELVLGGSDPAHYIPPLTFVPVTVPAYWQIHMERVKVGPGLTLCAKGCAAILDTGTSLITGPTEEIRALHAAIGGIPLLAGEYIILCSEIPKLPAVSFLLGGVWFNLTAHDYVIQALDVPPPAGPFWILGDVFLGTYVAVFDRGDMKSSARVGLARARTRGADLGWGETAQAQFPG","length":406,"molWeight":43853,"crc64":"F5E81F3102F24F8D","md5":"CF73DB71B4725833E5F7314DB21D147B"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Signal":1,"Chain":1,"Domain":1,"Active site":2},"uniParcId":"UPI0040316CC7"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0ACI8RNL8","uniProtkbId":"A0ACI8RNL8_HUMAN","entryAudit":{"firstPublicDate":"2026-06-10","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2026-06-10","entryVersion":2,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Homo sapiens","commonName":"Human","taxonId":9606,"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522750.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}],"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Primates","Haplorrhini","Catarrhini","Hominidae","Homo"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}],"value":"Pepsin A"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}],"value":"3.4.23.1"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522750.1"}],"value":"NAPSA"}}],"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.","ecNumber":"3.4.23.1","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007447"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000454"}],"value":"Belongs to the peptidase A1 family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":24,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}]},{"type":"Chain","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":339,"modifier":"EXACT"}},"description":"Pepsin A","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}],"featureId":"PRO_5051485927"},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":318,"modifier":"EXACT"}},"description":"Peptidase A1","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51767"}]},{"type":"Active site","location":{"start":{"value":202,"modifier":"EXACT"},"end":{"value":202,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022750"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000454"}],"id":"KW-0064","category":"Molecular function","name":"Aspartyl protease"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023157"}],"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023180"}],"id":"KW-0325","category":"PTM","name":"Glycoprotein"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022801"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000454"}],"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022670"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000454"}],"id":"KW-0645","category":"Molecular function","name":"Protease"},{"evidences":[{"evidenceCode":"ECO:0007829","source":"PeptideAtlas","id":"A0ACI8RNL8"},{"evidenceCode":"ECO:0007829","source":"ProteomicsDB","id":"A0ACI8RNL8"}],"id":"KW-1267","category":"Technical term","name":"Proteomics identification"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022729"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}],"id":"KW-0732","category":"Domain","name":"Signal"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023145"}],"id":"KW-0865","category":"PTM","name":"Zymogen"}],"references":[{"referenceNumber":1,"citation":{"id":"11237011","citationType":"journal article","authoringGroup":["International Human Genome Sequencing Consortium"],"authors":["Lander E.S.","Linton L.M.","Birren B.","Nusbaum C.","Zody M.C.","Baldwin J.","Devon K.","Dewar K.","Doyle M.","FitzHugh W.","Funke R.","Gage D.","Harris K.","Heaford A.","Howland J.","Kann L.","Lehoczky J.","LeVine R.","McEwan P.","McKernan K.","Meldrim J.","Mesirov J.P.","Miranda C.","Morris W.","Naylor J.","Raymond C.","Rosetti M.","Santos R.","Sheridan A.","Sougnez C.","Stange-Thomann N.","Stojanovic N.","Subramanian A.","Wyman D.","Rogers J.","Sulston J.","Ainscough R.","Beck S.","Bentley D.","Burton J.","Clee C.","Carter N.","Coulson A.","Deadman R.","Deloukas P.","Dunham A.","Dunham I.","Durbin R.","French L.","Grafham D.","Gregory S.","Hubbard T.","Humphray S.","Hunt A.","Jones M.","Lloyd C.","McMurray A.","Matthews L.","Mercer S.","Milne S.","Mullikin J.C.","Mungall A.","Plumb R.","Ross M.","Shownkeen R.","Sims S.","Waterston R.H.","Wilson R.K.","Hillier L.W.","McPherson J.D.","Marra M.A.","Mardis E.R.","Fulton L.A.","Chinwalla A.T.","Pepin K.H.","Gish W.R.","Chissoe S.L.","Wendl M.C.","Delehaunty K.D.","Miner T.L.","Delehaunty A.","Kramer J.B.","Cook L.L.","Fulton R.S.","Johnson D.L.","Minx P.J.","Clifton S.W.","Hawkins T.","Branscomb E.","Predki P.","Richardson P.","Wenning S.","Slezak T.","Doggett N.","Cheng J.F.","Olsen A.","Lucas S.","Elkin C.","Uberbacher E.","Frazier M.","Gibbs R.A.","Muzny D.M.","Scherer S.E.","Bouck J.B.","Sodergren E.J.","Worley K.C.","Rives C.M.","Gorrell J.H.","Metzker M.L.","Naylor S.L.","Kucherlapati R.S.","Nelson D.L.","Weinstock G.M.","Sakaki Y.","Fujiyama A.","Hattori M.","Yada T.","Toyoda A.","Itoh T.","Kawagoe C.","Watanabe H.","Totoki Y.","Taylor T.","Weissenbach J.","Heilig R.","Saurin W.","Artiguenave F.","Brottier P.","Bruls T.","Pelletier E.","Robert C.","Wincker P.","Smith D.R.","Doucette-Stamm L.","Rubenfield M.","Weinstock K.","Lee H.M.","Dubois J.","Rosenthal A.","Platzer M.","Nyakatura G.","Taudien S.","Rump A.","Yang H.","Yu J.","Wang J.","Huang G.","Gu J.","Hood L.","Rowen L.","Madan A.","Qin S.","Davis R.W.","Federspiel N.A.","Abola A.P.","Proctor M.J.","Myers R.M.","Schmutz J.","Dickson M.","Grimwood J.","Cox D.R.","Olson M.V.","Kaul R.","Raymond C.","Shimizu N.","Kawasaki K.","Minoshima S.","Evans G.A.","Athanasiou M.","Schultz R.","Roe B.A.","Chen F.","Pan H.","Ramser J.","Lehrach H.","Reinhardt R.","McCombie W.R.","de la Bastide M.","Dedhia N.","Blocker H.","Hornischer K.","Nordsiek G.","Agarwala R.","Aravind L.","Bailey J.A.","Bateman A.","Batzoglou S.","Birney E.","Bork P.","Brown D.G.","Burge C.B.","Cerutti L.","Chen H.C.","Church D.","Clamp M.","Copley R.R.","Doerks T.","Eddy S.R.","Eichler E.E.","Furey T.S.","Galagan J.","Gilbert J.G.","Harmon C.","Hayashizaki Y.","Haussler D.","Hermjakob H.","Hokamp K.","Jang W.","Johnson L.S.","Jones T.A.","Kasif S.","Kaspryzk A.","Kennedy S.","Kent W.J.","Kitts P.","Koonin E.V.","Korf I.","Kulp D.","Lancet D.","Lowe T.M.","McLysaght A.","Mikkelsen T.","Moran J.V.","Mulder N.","Pollara V.J.","Ponting C.P.","Schuler G.","Schultz J.","Slater G.","Smit A.F.","Stupka E.","Szustakowski J.","Thierry-Mieg D.","Thierry-Mieg J.","Wagner L.","Wallis J.","Wheeler R.","Williams A.","Wolf Y.I.","Wolfe K.H.","Yang S.P.","Yeh R.F.","Collins F.","Guyer M.S.","Peterson J.","Felsenfeld A.","Wetterstrand K.A.","Patrinos A.","Morgan M.J.","de Jong P.","Catanese J.J.","Osoegawa K.","Shizuya H.","Choi S.","Chen Y.J."],"citationCrossReferences":[{"database":"PubMed","id":"11237011"},{"database":"DOI","id":"10.1038/35057062"}],"title":"Initial sequencing and analysis of the human genome.","publicationDate":"2001","journal":"Nature","firstPage":"860","lastPage":"921","volume":"409"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522750.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":2,"citation":{"id":"15057824","citationType":"journal article","authors":["Grimwood J.","Gordon L.A.","Olsen A.","Terry A.","Schmutz J.","Lamerdin J.","Hellsten U.","Goodstein D.","Couronne O.","Tran-Gyamfi M.","Aerts A.","Altherr M.","Ashworth L.","Bajorek E.","Black S.","Branscomb E.","Caenepeel S.","Carrano A.","Caoile C.","Chan Y.M.","Christensen M.","Cleland C.A.","Copeland A.","Dalin E.","Dehal P.","Denys M.","Detter J.C.","Escobar J.","Flowers D.","Fotopulos D.","Garcia C.","Georgescu A.M.","Glavina T.","Gomez M.","Gonzales E.","Groza M.","Hammon N.","Hawkins T.","Haydu L.","Ho I.","Huang W.","Israni S.","Jett J.","Kadner K.","Kimball H.","Kobayashi A.","Larionov V.","Leem S.H.","Lopez F.","Lou Y.","Lowry S.","Malfatti S.","Martinez D.","McCready P.","Medina C.","Morgan J.","Nelson K.","Nolan M.","Ovcharenko I.","Pitluck S.","Pollard M.","Popkie A.P.","Predki P.","Quan G.","Ramirez L.","Rash S.","Retterer J.","Rodriguez A.","Rogers S.","Salamov A.","Salazar A.","She X.","Smith D.","Slezak T.","Solovyev V.","Thayer N.","Tice H.","Tsai M.","Ustaszewska A.","Vo N.","Wagner M.","Wheeler J.","Wu K.","Xie G.","Yang J.","Dubchak I.","Furey T.S.","DeJong P.","Dickson M.","Gordon D.","Eichler E.E.","Pennacchio L.A.","Richardson P.","Stubbs L.","Rokhsar D.S.","Myers R.M.","Rubin E.M.","Lucas S.M."],"citationCrossReferences":[{"database":"PubMed","id":"15057824"},{"database":"DOI","id":"10.1038/nature02399"}],"title":"The DNA sequence and biology of human chromosome 19.","publicationDate":"2004","journal":"Nature","firstPage":"529","lastPage":"535","volume":"428"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522750.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":3,"citation":{"id":"15496913","citationType":"journal article","authoringGroup":["International Human Genome Sequencing Consortium"],"citationCrossReferences":[{"database":"PubMed","id":"15496913"},{"database":"DOI","id":"10.1038/nature03001"}],"title":"Finishing the euchromatic sequence of the human genome.","publicationDate":"2004","journal":"Nature","firstPage":"931","lastPage":"945","volume":"431"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522750.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":4,"citation":{"id":"CI-20IM145UGQVF7","citationType":"submission","authoringGroup":["Ensembl"],"publicationDate":"JAN-2026","submissionDatabase":"UniProtKB"},"referencePositions":["IDENTIFICATION"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522750.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AC008655","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"Ensembl","id":"ENST00000852691.1","properties":[{"key":"ProteinId","value":"ENSP00000522750.1"},{"key":"GeneId","value":"ENSG00000131400.10"}]},{"database":"HGNC","id":"HGNC:13395","properties":[{"key":"GeneName","value":"NAPSA"}]},{"database":"OpenTargets","id":"ENSG00000131400","properties":[{"key":"Description","value":"-"}]},{"database":"GeneTree","id":"ENSGT00940000160179","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000005640","properties":[{"key":"Component","value":"Chromosome 19"}]},{"database":"FunFam","id":"2.40.70.10:FF:000066","properties":[{"key":"EntryName","value":"Napsin A aspartic peptidase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.70.10","properties":[{"key":"EntryName","value":"Acid Proteases"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR001461","properties":[{"key":"EntryName","value":"Aspartic_peptidase_A1"}]},{"database":"InterPro","id":"IPR001969","properties":[{"key":"EntryName","value":"Aspartic_peptidase_AS"}]},{"database":"InterPro","id":"IPR033121","properties":[{"key":"EntryName","value":"PEPTIDASE_A1"}]},{"database":"InterPro","id":"IPR021109","properties":[{"key":"EntryName","value":"Peptidase_aspartic_dom_sf"}]},{"database":"PANTHER","id":"PTHR47966","properties":[{"key":"EntryName","value":"BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR47966:SF83","properties":[{"key":"EntryName","value":"NAPSIN-A"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00026","properties":[{"key":"EntryName","value":"Asp"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00792","properties":[{"key":"EntryName","value":"PEPSIN"}]},{"database":"SUPFAM","id":"SSF50630","properties":[{"key":"EntryName","value":"Acid proteases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00141","properties":[{"key":"EntryName","value":"ASP_PROTEASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51767","properties":[{"key":"EntryName","value":"PEPTIDASE_A1"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSPPPLLQPLLLLLPLLNVEPSGATLIRIPLHRVQPGRRILNLLRGWREPAELPKLGAPSPGDKPIFVPLSNYRDIGGIKGASVIFGEALWEPSLVFAFAHFDGILGLGFPILSVEGVRPPMDVLVEQGLLDKPVFSFYLNRDPEEPDGGELVLGGSDPAHYIPPLTFVPVTVPAYWQIHMERVKVGPGLTLCAKGCAAILDTGTSLITGPTEEIRALHAAIGGIPLLAGEYIILCSEIPKLPAVSFLLGGVWFNLTAHDYVIQTTRNGVRLCLSGFQALDVPPPAGPFWILGDVFLGTYVAVFDRGDMKSSARVGLARARTRGADLGWGETAQAQFPG","length":339,"molWeight":36379,"crc64":"EAD089DE1DA138B0","md5":"DF0D3EACFB7EDE0CAF0BE3151CD61F26"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Signal":1,"Chain":1,"Domain":1,"Active site":1},"uniParcId":"UPI004032159E"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0ACI8VG40","uniProtkbId":"A0ACI8VG40_HUMAN","entryAudit":{"firstPublicDate":"2026-06-10","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2026-06-10","entryVersion":2,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Homo sapiens","commonName":"Human","taxonId":9606,"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000637999.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}],"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Primates","Haplorrhini","Catarrhini","Hominidae","Homo"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}],"value":"Pepsin A"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}],"value":"3.4.23.1"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000637999.1"}],"value":"NAPSA"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00055304"}],"value":"May be involved in processing of pneumocyte surfactant precursors"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.","ecNumber":"3.4.23.1","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004613"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007447"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000454"}],"value":"Belongs to the peptidase A1 family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":24,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}]},{"type":"Chain","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":415,"modifier":"EXACT"}},"description":"Pepsin A","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}],"featureId":"PRO_5051406035"},{"type":"Domain","location":{"start":{"value":78,"modifier":"EXACT"},"end":{"value":394,"modifier":"EXACT"}},"description":"Peptidase A1","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51767"}]},{"type":"Active site","location":{"start":{"value":96,"modifier":"EXACT"},"end":{"value":96,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}]},{"type":"Active site","location":{"start":{"value":278,"modifier":"EXACT"},"end":{"value":278,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022750"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"id":"KW-0064","category":"Molecular function","name":"Aspartyl protease"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023157"}],"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023180"}],"id":"KW-0325","category":"PTM","name":"Glycoprotein"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022801"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022670"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"id":"KW-0645","category":"Molecular function","name":"Protease"},{"evidences":[{"evidenceCode":"ECO:0007829","source":"PeptideAtlas","id":"A0ACI8VG40"},{"evidenceCode":"ECO:0007829","source":"ProteomicsDB","id":"A0ACI8VG40"}],"id":"KW-1267","category":"Technical term","name":"Proteomics identification"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022525"}],"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022729"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}],"id":"KW-0732","category":"Domain","name":"Signal"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023145"}],"id":"KW-0865","category":"PTM","name":"Zymogen"}],"references":[{"referenceNumber":1,"citation":{"id":"11237011","citationType":"journal article","authoringGroup":["International Human Genome Sequencing Consortium"],"authors":["Lander E.S.","Linton L.M.","Birren B.","Nusbaum C.","Zody M.C.","Baldwin J.","Devon K.","Dewar K.","Doyle M.","FitzHugh W.","Funke R.","Gage D.","Harris K.","Heaford A.","Howland J.","Kann L.","Lehoczky J.","LeVine R.","McEwan P.","McKernan K.","Meldrim J.","Mesirov J.P.","Miranda C.","Morris W.","Naylor J.","Raymond C.","Rosetti M.","Santos R.","Sheridan A.","Sougnez C.","Stange-Thomann N.","Stojanovic N.","Subramanian A.","Wyman D.","Rogers J.","Sulston J.","Ainscough R.","Beck S.","Bentley D.","Burton J.","Clee C.","Carter N.","Coulson A.","Deadman R.","Deloukas P.","Dunham A.","Dunham I.","Durbin R.","French L.","Grafham D.","Gregory S.","Hubbard T.","Humphray S.","Hunt A.","Jones M.","Lloyd C.","McMurray A.","Matthews L.","Mercer S.","Milne S.","Mullikin J.C.","Mungall A.","Plumb R.","Ross M.","Shownkeen R.","Sims S.","Waterston R.H.","Wilson R.K.","Hillier L.W.","McPherson J.D.","Marra M.A.","Mardis E.R.","Fulton L.A.","Chinwalla A.T.","Pepin K.H.","Gish W.R.","Chissoe S.L.","Wendl M.C.","Delehaunty K.D.","Miner T.L.","Delehaunty A.","Kramer J.B.","Cook L.L.","Fulton R.S.","Johnson D.L.","Minx P.J.","Clifton S.W.","Hawkins T.","Branscomb E.","Predki P.","Richardson P.","Wenning S.","Slezak T.","Doggett N.","Cheng J.F.","Olsen A.","Lucas S.","Elkin C.","Uberbacher E.","Frazier M.","Gibbs R.A.","Muzny D.M.","Scherer S.E.","Bouck J.B.","Sodergren E.J.","Worley K.C.","Rives C.M.","Gorrell J.H.","Metzker M.L.","Naylor S.L.","Kucherlapati R.S.","Nelson D.L.","Weinstock G.M.","Sakaki Y.","Fujiyama A.","Hattori M.","Yada T.","Toyoda A.","Itoh T.","Kawagoe C.","Watanabe H.","Totoki Y.","Taylor T.","Weissenbach J.","Heilig R.","Saurin W.","Artiguenave F.","Brottier P.","Bruls T.","Pelletier E.","Robert C.","Wincker P.","Smith D.R.","Doucette-Stamm L.","Rubenfield M.","Weinstock K.","Lee H.M.","Dubois J.","Rosenthal A.","Platzer M.","Nyakatura G.","Taudien S.","Rump A.","Yang H.","Yu J.","Wang J.","Huang G.","Gu J.","Hood L.","Rowen L.","Madan A.","Qin S.","Davis R.W.","Federspiel N.A.","Abola A.P.","Proctor M.J.","Myers R.M.","Schmutz J.","Dickson M.","Grimwood J.","Cox D.R.","Olson M.V.","Kaul R.","Raymond C.","Shimizu N.","Kawasaki K.","Minoshima S.","Evans G.A.","Athanasiou M.","Schultz R.","Roe B.A.","Chen F.","Pan H.","Ramser J.","Lehrach H.","Reinhardt R.","McCombie W.R.","de la Bastide M.","Dedhia N.","Blocker H.","Hornischer K.","Nordsiek G.","Agarwala R.","Aravind L.","Bailey J.A.","Bateman A.","Batzoglou S.","Birney E.","Bork P.","Brown D.G.","Burge C.B.","Cerutti L.","Chen H.C.","Church D.","Clamp M.","Copley R.R.","Doerks T.","Eddy S.R.","Eichler E.E.","Furey T.S.","Galagan J.","Gilbert J.G.","Harmon C.","Hayashizaki Y.","Haussler D.","Hermjakob H.","Hokamp K.","Jang W.","Johnson L.S.","Jones T.A.","Kasif S.","Kaspryzk A.","Kennedy S.","Kent W.J.","Kitts P.","Koonin E.V.","Korf I.","Kulp D.","Lancet D.","Lowe T.M.","McLysaght A.","Mikkelsen T.","Moran J.V.","Mulder N.","Pollara V.J.","Ponting C.P.","Schuler G.","Schultz J.","Slater G.","Smit A.F.","Stupka E.","Szustakowski J.","Thierry-Mieg D.","Thierry-Mieg J.","Wagner L.","Wallis J.","Wheeler R.","Williams A.","Wolf Y.I.","Wolfe K.H.","Yang S.P.","Yeh R.F.","Collins F.","Guyer M.S.","Peterson J.","Felsenfeld A.","Wetterstrand K.A.","Patrinos A.","Morgan M.J.","de Jong P.","Catanese J.J.","Osoegawa K.","Shizuya H.","Choi S.","Chen Y.J."],"citationCrossReferences":[{"database":"PubMed","id":"11237011"},{"database":"DOI","id":"10.1038/35057062"}],"title":"Initial sequencing and analysis of the human genome.","publicationDate":"2001","journal":"Nature","firstPage":"860","lastPage":"921","volume":"409"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000637999.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":2,"citation":{"id":"15057824","citationType":"journal article","authors":["Grimwood J.","Gordon L.A.","Olsen A.","Terry A.","Schmutz J.","Lamerdin J.","Hellsten U.","Goodstein D.","Couronne O.","Tran-Gyamfi M.","Aerts A.","Altherr M.","Ashworth L.","Bajorek E.","Black S.","Branscomb E.","Caenepeel S.","Carrano A.","Caoile C.","Chan Y.M.","Christensen M.","Cleland C.A.","Copeland A.","Dalin E.","Dehal P.","Denys M.","Detter J.C.","Escobar J.","Flowers D.","Fotopulos D.","Garcia C.","Georgescu A.M.","Glavina T.","Gomez M.","Gonzales E.","Groza M.","Hammon N.","Hawkins T.","Haydu L.","Ho I.","Huang W.","Israni S.","Jett J.","Kadner K.","Kimball H.","Kobayashi A.","Larionov V.","Leem S.H.","Lopez F.","Lou Y.","Lowry S.","Malfatti S.","Martinez D.","McCready P.","Medina C.","Morgan J.","Nelson K.","Nolan M.","Ovcharenko I.","Pitluck S.","Pollard M.","Popkie A.P.","Predki P.","Quan G.","Ramirez L.","Rash S.","Retterer J.","Rodriguez A.","Rogers S.","Salamov A.","Salazar A.","She X.","Smith D.","Slezak T.","Solovyev V.","Thayer N.","Tice H.","Tsai M.","Ustaszewska A.","Vo N.","Wagner M.","Wheeler J.","Wu K.","Xie G.","Yang J.","Dubchak I.","Furey T.S.","DeJong P.","Dickson M.","Gordon D.","Eichler E.E.","Pennacchio L.A.","Richardson P.","Stubbs L.","Rokhsar D.S.","Myers R.M.","Rubin E.M.","Lucas S.M."],"citationCrossReferences":[{"database":"PubMed","id":"15057824"},{"database":"DOI","id":"10.1038/nature02399"}],"title":"The DNA sequence and biology of human chromosome 19.","publicationDate":"2004","journal":"Nature","firstPage":"529","lastPage":"535","volume":"428"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000637999.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":3,"citation":{"id":"15496913","citationType":"journal article","authoringGroup":["International Human Genome Sequencing Consortium"],"citationCrossReferences":[{"database":"PubMed","id":"15496913"},{"database":"DOI","id":"10.1038/nature03001"}],"title":"Finishing the euchromatic sequence of the human genome.","publicationDate":"2004","journal":"Nature","firstPage":"931","lastPage":"945","volume":"431"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000637999.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":4,"citation":{"id":"CI-20IM145UGQVF7","citationType":"submission","authoringGroup":["Ensembl"],"publicationDate":"JAN-2026","submissionDatabase":"UniProtKB"},"referencePositions":["IDENTIFICATION"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000637999.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AC008655","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"Ensembl","id":"ENST00000967940.1","properties":[{"key":"ProteinId","value":"ENSP00000637999.1"},{"key":"GeneId","value":"ENSG00000131400.10"}]},{"database":"HGNC","id":"HGNC:13395","properties":[{"key":"GeneName","value":"NAPSA"}]},{"database":"OpenTargets","id":"ENSG00000131400","properties":[{"key":"Description","value":"-"}]},{"database":"GeneTree","id":"ENSGT00940000160179","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000005640","properties":[{"key":"Component","value":"Chromosome 19"}]},{"database":"FunFam","id":"2.40.70.10:FF:000048","properties":[{"key":"EntryName","value":"Napsin A aspartic peptidase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.40.70.10:FF:000066","properties":[{"key":"EntryName","value":"Napsin A aspartic peptidase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.70.10","properties":[{"key":"EntryName","value":"Acid Proteases"},{"key":"MatchStatus","value":"2"}]},{"database":"InterPro","id":"IPR001461","properties":[{"key":"EntryName","value":"Aspartic_peptidase_A1"}]},{"database":"InterPro","id":"IPR001969","properties":[{"key":"EntryName","value":"Aspartic_peptidase_AS"}]},{"database":"InterPro","id":"IPR033121","properties":[{"key":"EntryName","value":"PEPTIDASE_A1"}]},{"database":"InterPro","id":"IPR021109","properties":[{"key":"EntryName","value":"Peptidase_aspartic_dom_sf"}]},{"database":"PANTHER","id":"PTHR47966","properties":[{"key":"EntryName","value":"BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR47966:SF83","properties":[{"key":"EntryName","value":"NAPSIN-A"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00026","properties":[{"key":"EntryName","value":"Asp"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00792","properties":[{"key":"EntryName","value":"PEPSIN"}]},{"database":"SUPFAM","id":"SSF50630","properties":[{"key":"EntryName","value":"Acid proteases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00141","properties":[{"key":"EntryName","value":"ASP_PROTEASE"},{"key":"MatchStatus","value":"2"}]},{"database":"PROSITE","id":"PS51767","properties":[{"key":"EntryName","value":"PEPTIDASE_A1"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSPPPLLQPLLLLLPLLNVEPSGATLIRIPLHRVQPGRRILNLLRGWREPAELPKLGAPSPGDKPIFVPLSNYRDVQYFGEIGLGTPPQNFTVAFDTGSSNLWVPSRRCHFFSVPCWLHHRFDPKASSSFQANGTKFAIQYGTGRVDGILSEDKLTGASVIFGEALWEPSLVFAFAHFDGILGLGFPILSVEGVRPPMDVLVEQGLLDKPVFSFYLNRDPEEPDGGELVLGGSDPAHYIPPLTFVPVTVPAYWQIHMERVKVGPGLTLCAKGCAAILDTGTSLITGPTEEIRALHAAIGGIPLLAGEYIILCSEIPKLPAVSFLLGGVWFNLTAHDYVIQTTRNGVRLCLSGFQALDVPPPAGPFWILGDVFLGTYVAVFDRGDMKSSARVGLARARTRGADLGWGETAQAQFPG","length":415,"molWeight":44918,"crc64":"6DE8C0BC7131A5CD","md5":"6FDBE45CC99FD63A0D7E3AED213AFFA5"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Signal":1,"Chain":1,"Domain":1,"Active site":2},"uniParcId":"UPI004031427E"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0ACI8QSW0","uniProtkbId":"A0ACI8QSW0_HUMAN","entryAudit":{"firstPublicDate":"2026-06-10","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2026-06-10","entryVersion":2,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Homo sapiens","commonName":"Human","taxonId":9606,"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522749.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}],"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Primates","Haplorrhini","Catarrhini","Hominidae","Homo"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}],"value":"Pepsin A"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}],"value":"3.4.23.1"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522749.1"}],"value":"NAPSA"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00055304"}],"value":"May be involved in processing of pneumocyte surfactant precursors"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.","ecNumber":"3.4.23.1","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004613"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007447"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000454"}],"value":"Belongs to the peptidase A1 family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":24,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}]},{"type":"Chain","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":409,"modifier":"EXACT"}},"description":"Pepsin A","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}],"featureId":"PRO_5051464793"},{"type":"Domain","location":{"start":{"value":76,"modifier":"EXACT"},"end":{"value":388,"modifier":"EXACT"}},"description":"Peptidase A1","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51767"}]},{"type":"Active site","location":{"start":{"value":94,"modifier":"EXACT"},"end":{"value":94,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}]},{"type":"Active site","location":{"start":{"value":272,"modifier":"EXACT"},"end":{"value":272,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022750"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"id":"KW-0064","category":"Molecular function","name":"Aspartyl protease"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023157"}],"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023180"}],"id":"KW-0325","category":"PTM","name":"Glycoprotein"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022801"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022670"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"id":"KW-0645","category":"Molecular function","name":"Protease"},{"evidences":[{"evidenceCode":"ECO:0007829","source":"PeptideAtlas","id":"A0ACI8QSW0"},{"evidenceCode":"ECO:0007829","source":"ProteomicsDB","id":"A0ACI8QSW0"}],"id":"KW-1267","category":"Technical term","name":"Proteomics identification"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022525"}],"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022729"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}],"id":"KW-0732","category":"Domain","name":"Signal"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023145"}],"id":"KW-0865","category":"PTM","name":"Zymogen"}],"references":[{"referenceNumber":1,"citation":{"id":"11237011","citationType":"journal article","authoringGroup":["International Human Genome Sequencing Consortium"],"authors":["Lander E.S.","Linton L.M.","Birren B.","Nusbaum C.","Zody M.C.","Baldwin J.","Devon K.","Dewar K.","Doyle M.","FitzHugh W.","Funke R.","Gage D.","Harris K.","Heaford A.","Howland J.","Kann L.","Lehoczky J.","LeVine R.","McEwan P.","McKernan K.","Meldrim J.","Mesirov J.P.","Miranda C.","Morris W.","Naylor J.","Raymond C.","Rosetti M.","Santos R.","Sheridan A.","Sougnez C.","Stange-Thomann N.","Stojanovic N.","Subramanian A.","Wyman D.","Rogers J.","Sulston J.","Ainscough R.","Beck S.","Bentley D.","Burton J.","Clee C.","Carter N.","Coulson A.","Deadman R.","Deloukas P.","Dunham A.","Dunham I.","Durbin R.","French L.","Grafham D.","Gregory S.","Hubbard T.","Humphray S.","Hunt A.","Jones M.","Lloyd C.","McMurray A.","Matthews L.","Mercer S.","Milne S.","Mullikin J.C.","Mungall A.","Plumb R.","Ross M.","Shownkeen R.","Sims S.","Waterston R.H.","Wilson R.K.","Hillier L.W.","McPherson J.D.","Marra M.A.","Mardis E.R.","Fulton L.A.","Chinwalla A.T.","Pepin K.H.","Gish W.R.","Chissoe S.L.","Wendl M.C.","Delehaunty K.D.","Miner T.L.","Delehaunty A.","Kramer J.B.","Cook L.L.","Fulton R.S.","Johnson D.L.","Minx P.J.","Clifton S.W.","Hawkins T.","Branscomb E.","Predki P.","Richardson P.","Wenning S.","Slezak T.","Doggett N.","Cheng J.F.","Olsen A.","Lucas S.","Elkin C.","Uberbacher E.","Frazier M.","Gibbs R.A.","Muzny D.M.","Scherer S.E.","Bouck J.B.","Sodergren E.J.","Worley K.C.","Rives C.M.","Gorrell J.H.","Metzker M.L.","Naylor S.L.","Kucherlapati R.S.","Nelson D.L.","Weinstock G.M.","Sakaki Y.","Fujiyama A.","Hattori M.","Yada T.","Toyoda A.","Itoh T.","Kawagoe C.","Watanabe H.","Totoki Y.","Taylor T.","Weissenbach J.","Heilig R.","Saurin W.","Artiguenave F.","Brottier P.","Bruls T.","Pelletier E.","Robert C.","Wincker P.","Smith D.R.","Doucette-Stamm L.","Rubenfield M.","Weinstock K.","Lee H.M.","Dubois J.","Rosenthal A.","Platzer M.","Nyakatura G.","Taudien S.","Rump A.","Yang H.","Yu J.","Wang J.","Huang G.","Gu J.","Hood L.","Rowen L.","Madan A.","Qin S.","Davis R.W.","Federspiel N.A.","Abola A.P.","Proctor M.J.","Myers R.M.","Schmutz J.","Dickson M.","Grimwood J.","Cox D.R.","Olson M.V.","Kaul R.","Raymond C.","Shimizu N.","Kawasaki K.","Minoshima S.","Evans G.A.","Athanasiou M.","Schultz R.","Roe B.A.","Chen F.","Pan H.","Ramser J.","Lehrach H.","Reinhardt R.","McCombie W.R.","de la Bastide M.","Dedhia N.","Blocker H.","Hornischer K.","Nordsiek G.","Agarwala R.","Aravind L.","Bailey J.A.","Bateman A.","Batzoglou S.","Birney E.","Bork P.","Brown D.G.","Burge C.B.","Cerutti L.","Chen H.C.","Church D.","Clamp M.","Copley R.R.","Doerks T.","Eddy S.R.","Eichler E.E.","Furey T.S.","Galagan J.","Gilbert J.G.","Harmon C.","Hayashizaki Y.","Haussler D.","Hermjakob H.","Hokamp K.","Jang W.","Johnson L.S.","Jones T.A.","Kasif S.","Kaspryzk A.","Kennedy S.","Kent W.J.","Kitts P.","Koonin E.V.","Korf I.","Kulp D.","Lancet D.","Lowe T.M.","McLysaght A.","Mikkelsen T.","Moran J.V.","Mulder N.","Pollara V.J.","Ponting C.P.","Schuler G.","Schultz J.","Slater G.","Smit A.F.","Stupka E.","Szustakowski J.","Thierry-Mieg D.","Thierry-Mieg J.","Wagner L.","Wallis J.","Wheeler R.","Williams A.","Wolf Y.I.","Wolfe K.H.","Yang S.P.","Yeh R.F.","Collins F.","Guyer M.S.","Peterson J.","Felsenfeld A.","Wetterstrand K.A.","Patrinos A.","Morgan M.J.","de Jong P.","Catanese J.J.","Osoegawa K.","Shizuya H.","Choi S.","Chen Y.J."],"citationCrossReferences":[{"database":"PubMed","id":"11237011"},{"database":"DOI","id":"10.1038/35057062"}],"title":"Initial sequencing and analysis of the human genome.","publicationDate":"2001","journal":"Nature","firstPage":"860","lastPage":"921","volume":"409"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522749.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":2,"citation":{"id":"15057824","citationType":"journal article","authors":["Grimwood J.","Gordon L.A.","Olsen A.","Terry A.","Schmutz J.","Lamerdin J.","Hellsten U.","Goodstein D.","Couronne O.","Tran-Gyamfi M.","Aerts A.","Altherr M.","Ashworth L.","Bajorek E.","Black S.","Branscomb E.","Caenepeel S.","Carrano A.","Caoile C.","Chan Y.M.","Christensen M.","Cleland C.A.","Copeland A.","Dalin E.","Dehal P.","Denys M.","Detter J.C.","Escobar J.","Flowers D.","Fotopulos D.","Garcia C.","Georgescu A.M.","Glavina T.","Gomez M.","Gonzales E.","Groza M.","Hammon N.","Hawkins T.","Haydu L.","Ho I.","Huang W.","Israni S.","Jett J.","Kadner K.","Kimball H.","Kobayashi A.","Larionov V.","Leem S.H.","Lopez F.","Lou Y.","Lowry S.","Malfatti S.","Martinez D.","McCready P.","Medina C.","Morgan J.","Nelson K.","Nolan M.","Ovcharenko I.","Pitluck S.","Pollard M.","Popkie A.P.","Predki P.","Quan G.","Ramirez L.","Rash S.","Retterer J.","Rodriguez A.","Rogers S.","Salamov A.","Salazar A.","She X.","Smith D.","Slezak T.","Solovyev V.","Thayer N.","Tice H.","Tsai M.","Ustaszewska A.","Vo N.","Wagner M.","Wheeler J.","Wu K.","Xie G.","Yang J.","Dubchak I.","Furey T.S.","DeJong P.","Dickson M.","Gordon D.","Eichler E.E.","Pennacchio L.A.","Richardson P.","Stubbs L.","Rokhsar D.S.","Myers R.M.","Rubin E.M.","Lucas S.M."],"citationCrossReferences":[{"database":"PubMed","id":"15057824"},{"database":"DOI","id":"10.1038/nature02399"}],"title":"The DNA sequence and biology of human chromosome 19.","publicationDate":"2004","journal":"Nature","firstPage":"529","lastPage":"535","volume":"428"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522749.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":3,"citation":{"id":"15496913","citationType":"journal article","authoringGroup":["International Human Genome Sequencing Consortium"],"citationCrossReferences":[{"database":"PubMed","id":"15496913"},{"database":"DOI","id":"10.1038/nature03001"}],"title":"Finishing the euchromatic sequence of the human genome.","publicationDate":"2004","journal":"Nature","firstPage":"931","lastPage":"945","volume":"431"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522749.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":4,"citation":{"id":"CI-20IM145UGQVF7","citationType":"submission","authoringGroup":["Ensembl"],"publicationDate":"JAN-2026","submissionDatabase":"UniProtKB"},"referencePositions":["IDENTIFICATION"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522749.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AC008655","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"Ensembl","id":"ENST00000852690.1","properties":[{"key":"ProteinId","value":"ENSP00000522749.1"},{"key":"GeneId","value":"ENSG00000131400.10"}]},{"database":"HGNC","id":"HGNC:13395","properties":[{"key":"GeneName","value":"NAPSA"}]},{"database":"OpenTargets","id":"ENSG00000131400","properties":[{"key":"Description","value":"-"}]},{"database":"GeneTree","id":"ENSGT00940000160179","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000005640","properties":[{"key":"Component","value":"Chromosome 19"}]},{"database":"FunFam","id":"2.40.70.10:FF:000048","properties":[{"key":"EntryName","value":"Napsin A aspartic peptidase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.40.70.10:FF:000066","properties":[{"key":"EntryName","value":"Napsin A aspartic peptidase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.70.10","properties":[{"key":"EntryName","value":"Acid Proteases"},{"key":"MatchStatus","value":"3"}]},{"database":"InterPro","id":"IPR001461","properties":[{"key":"EntryName","value":"Aspartic_peptidase_A1"}]},{"database":"InterPro","id":"IPR001969","properties":[{"key":"EntryName","value":"Aspartic_peptidase_AS"}]},{"database":"InterPro","id":"IPR033121","properties":[{"key":"EntryName","value":"PEPTIDASE_A1"}]},{"database":"InterPro","id":"IPR021109","properties":[{"key":"EntryName","value":"Peptidase_aspartic_dom_sf"}]},{"database":"PANTHER","id":"PTHR47966","properties":[{"key":"EntryName","value":"BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR47966:SF83","properties":[{"key":"EntryName","value":"NAPSIN-A"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00026","properties":[{"key":"EntryName","value":"Asp"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00792","properties":[{"key":"EntryName","value":"PEPSIN"}]},{"database":"SUPFAM","id":"SSF50630","properties":[{"key":"EntryName","value":"Acid proteases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00141","properties":[{"key":"EntryName","value":"ASP_PROTEASE"},{"key":"MatchStatus","value":"2"}]},{"database":"PROSITE","id":"PS51767","properties":[{"key":"EntryName","value":"PEPTIDASE_A1"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSPPPLLQPLLLLLPLLNVEPSGATLIRIPLHRVQPGRRILNLLRGWREPAELPKLGAPSPGDKPIFVPLSNYRDYFGEIGLGTPPQNFTVAFDTGSSNLWVPSRRCHFFSVPCSSSSFQANGTKFAIQYGTGRVDGILSEDKLTIGGIKGASVIFGEALWEPSLVFAFAHFDGILGLGFPILSVEGVRPPMDVLVEQGLLDKPVFSFYLNRDPEEPDGGELVLGGSDPAHYIPPLTFVPVTVPAYWQIHMERVKVGPGLTLCAKGCAAILDTGTSLITGPTEEIRALHAAIGGIPLLAGEYIILCSEIPKLPAVSFLLGGVWFNLTAHDYVIQTTRNGVRLCLSGFQALDVPPPAGPFWILGDVFLGTYVAVFDRGDMKSSARVGLARARTRGADLGWGETAQAQFPG","length":409,"molWeight":43958,"crc64":"ABFCFB3343344AD2","md5":"0C8A4EE7F49C5B10A7737FAE50EEC2DA"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Signal":1,"Chain":1,"Domain":1,"Active site":2},"uniParcId":"UPI004031AAA7"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0ACI8QMA2","uniProtkbId":"A0ACI8QMA2_HUMAN","entryAudit":{"firstPublicDate":"2026-06-10","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2026-06-10","entryVersion":2,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Homo sapiens","commonName":"Human","taxonId":9606,"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522746.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}],"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Primates","Haplorrhini","Catarrhini","Hominidae","Homo"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}],"value":"Pepsin A"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}],"value":"3.4.23.1"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522746.1"}],"value":"NAPSA"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00055304"}],"value":"May be involved in processing of pneumocyte surfactant precursors"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.","ecNumber":"3.4.23.1","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004613"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007447"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000454"}],"value":"Belongs to the peptidase A1 family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":24,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}]},{"type":"Chain","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":418,"modifier":"EXACT"}},"description":"Pepsin A","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}],"featureId":"PRO_5051235732"},{"type":"Domain","location":{"start":{"value":76,"modifier":"EXACT"},"end":{"value":397,"modifier":"EXACT"}},"description":"Peptidase A1","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51767"}]},{"type":"Active site","location":{"start":{"value":94,"modifier":"EXACT"},"end":{"value":94,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}]},{"type":"Active site","location":{"start":{"value":281,"modifier":"EXACT"},"end":{"value":281,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022750"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"id":"KW-0064","category":"Molecular function","name":"Aspartyl protease"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023157"}],"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023180"}],"id":"KW-0325","category":"PTM","name":"Glycoprotein"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022801"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022670"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"id":"KW-0645","category":"Molecular function","name":"Protease"},{"evidences":[{"evidenceCode":"ECO:0007829","source":"PeptideAtlas","id":"A0ACI8QMA2"},{"evidenceCode":"ECO:0007829","source":"ProteomicsDB","id":"A0ACI8QMA2"}],"id":"KW-1267","category":"Technical term","name":"Proteomics identification"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022525"}],"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022729"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}],"id":"KW-0732","category":"Domain","name":"Signal"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023145"}],"id":"KW-0865","category":"PTM","name":"Zymogen"}],"references":[{"referenceNumber":1,"citation":{"id":"11237011","citationType":"journal article","authoringGroup":["International Human Genome Sequencing Consortium"],"authors":["Lander E.S.","Linton L.M.","Birren B.","Nusbaum C.","Zody M.C.","Baldwin J.","Devon K.","Dewar K.","Doyle M.","FitzHugh W.","Funke R.","Gage D.","Harris K.","Heaford A.","Howland J.","Kann L.","Lehoczky J.","LeVine R.","McEwan P.","McKernan K.","Meldrim J.","Mesirov J.P.","Miranda C.","Morris W.","Naylor J.","Raymond C.","Rosetti M.","Santos R.","Sheridan A.","Sougnez C.","Stange-Thomann N.","Stojanovic N.","Subramanian A.","Wyman D.","Rogers J.","Sulston J.","Ainscough R.","Beck S.","Bentley D.","Burton J.","Clee C.","Carter N.","Coulson A.","Deadman R.","Deloukas P.","Dunham A.","Dunham I.","Durbin R.","French L.","Grafham D.","Gregory S.","Hubbard T.","Humphray S.","Hunt A.","Jones M.","Lloyd C.","McMurray A.","Matthews L.","Mercer S.","Milne S.","Mullikin J.C.","Mungall A.","Plumb R.","Ross M.","Shownkeen R.","Sims S.","Waterston R.H.","Wilson R.K.","Hillier L.W.","McPherson J.D.","Marra M.A.","Mardis E.R.","Fulton L.A.","Chinwalla A.T.","Pepin K.H.","Gish W.R.","Chissoe S.L.","Wendl M.C.","Delehaunty K.D.","Miner T.L.","Delehaunty A.","Kramer J.B.","Cook L.L.","Fulton R.S.","Johnson D.L.","Minx P.J.","Clifton S.W.","Hawkins T.","Branscomb E.","Predki P.","Richardson P.","Wenning S.","Slezak T.","Doggett N.","Cheng J.F.","Olsen A.","Lucas S.","Elkin C.","Uberbacher E.","Frazier M.","Gibbs R.A.","Muzny D.M.","Scherer S.E.","Bouck J.B.","Sodergren E.J.","Worley K.C.","Rives C.M.","Gorrell J.H.","Metzker M.L.","Naylor S.L.","Kucherlapati R.S.","Nelson D.L.","Weinstock G.M.","Sakaki Y.","Fujiyama A.","Hattori M.","Yada T.","Toyoda A.","Itoh T.","Kawagoe C.","Watanabe H.","Totoki Y.","Taylor T.","Weissenbach J.","Heilig R.","Saurin W.","Artiguenave F.","Brottier P.","Bruls T.","Pelletier E.","Robert C.","Wincker P.","Smith D.R.","Doucette-Stamm L.","Rubenfield M.","Weinstock K.","Lee H.M.","Dubois J.","Rosenthal A.","Platzer M.","Nyakatura G.","Taudien S.","Rump A.","Yang H.","Yu J.","Wang J.","Huang G.","Gu J.","Hood L.","Rowen L.","Madan A.","Qin S.","Davis R.W.","Federspiel N.A.","Abola A.P.","Proctor M.J.","Myers R.M.","Schmutz J.","Dickson M.","Grimwood J.","Cox D.R.","Olson M.V.","Kaul R.","Raymond C.","Shimizu N.","Kawasaki K.","Minoshima S.","Evans G.A.","Athanasiou M.","Schultz R.","Roe B.A.","Chen F.","Pan H.","Ramser J.","Lehrach H.","Reinhardt R.","McCombie W.R.","de la Bastide M.","Dedhia N.","Blocker H.","Hornischer K.","Nordsiek G.","Agarwala R.","Aravind L.","Bailey J.A.","Bateman A.","Batzoglou S.","Birney E.","Bork P.","Brown D.G.","Burge C.B.","Cerutti L.","Chen H.C.","Church D.","Clamp M.","Copley R.R.","Doerks T.","Eddy S.R.","Eichler E.E.","Furey T.S.","Galagan J.","Gilbert J.G.","Harmon C.","Hayashizaki Y.","Haussler D.","Hermjakob H.","Hokamp K.","Jang W.","Johnson L.S.","Jones T.A.","Kasif S.","Kaspryzk A.","Kennedy S.","Kent W.J.","Kitts P.","Koonin E.V.","Korf I.","Kulp D.","Lancet D.","Lowe T.M.","McLysaght A.","Mikkelsen T.","Moran J.V.","Mulder N.","Pollara V.J.","Ponting C.P.","Schuler G.","Schultz J.","Slater G.","Smit A.F.","Stupka E.","Szustakowski J.","Thierry-Mieg D.","Thierry-Mieg J.","Wagner L.","Wallis J.","Wheeler R.","Williams A.","Wolf Y.I.","Wolfe K.H.","Yang S.P.","Yeh R.F.","Collins F.","Guyer M.S.","Peterson J.","Felsenfeld A.","Wetterstrand K.A.","Patrinos A.","Morgan M.J.","de Jong P.","Catanese J.J.","Osoegawa K.","Shizuya H.","Choi S.","Chen Y.J."],"citationCrossReferences":[{"database":"PubMed","id":"11237011"},{"database":"DOI","id":"10.1038/35057062"}],"title":"Initial sequencing and analysis of the human genome.","publicationDate":"2001","journal":"Nature","firstPage":"860","lastPage":"921","volume":"409"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522746.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":2,"citation":{"id":"15057824","citationType":"journal article","authors":["Grimwood J.","Gordon L.A.","Olsen A.","Terry A.","Schmutz J.","Lamerdin J.","Hellsten U.","Goodstein D.","Couronne O.","Tran-Gyamfi M.","Aerts A.","Altherr M.","Ashworth L.","Bajorek E.","Black S.","Branscomb E.","Caenepeel S.","Carrano A.","Caoile C.","Chan Y.M.","Christensen M.","Cleland C.A.","Copeland A.","Dalin E.","Dehal P.","Denys M.","Detter J.C.","Escobar J.","Flowers D.","Fotopulos D.","Garcia C.","Georgescu A.M.","Glavina T.","Gomez M.","Gonzales E.","Groza M.","Hammon N.","Hawkins T.","Haydu L.","Ho I.","Huang W.","Israni S.","Jett J.","Kadner K.","Kimball H.","Kobayashi A.","Larionov V.","Leem S.H.","Lopez F.","Lou Y.","Lowry S.","Malfatti S.","Martinez D.","McCready P.","Medina C.","Morgan J.","Nelson K.","Nolan M.","Ovcharenko I.","Pitluck S.","Pollard M.","Popkie A.P.","Predki P.","Quan G.","Ramirez L.","Rash S.","Retterer J.","Rodriguez A.","Rogers S.","Salamov A.","Salazar A.","She X.","Smith D.","Slezak T.","Solovyev V.","Thayer N.","Tice H.","Tsai M.","Ustaszewska A.","Vo N.","Wagner M.","Wheeler J.","Wu K.","Xie G.","Yang J.","Dubchak I.","Furey T.S.","DeJong P.","Dickson M.","Gordon D.","Eichler E.E.","Pennacchio L.A.","Richardson P.","Stubbs L.","Rokhsar D.S.","Myers R.M.","Rubin E.M.","Lucas S.M."],"citationCrossReferences":[{"database":"PubMed","id":"15057824"},{"database":"DOI","id":"10.1038/nature02399"}],"title":"The DNA sequence and biology of human chromosome 19.","publicationDate":"2004","journal":"Nature","firstPage":"529","lastPage":"535","volume":"428"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522746.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":3,"citation":{"id":"15496913","citationType":"journal article","authoringGroup":["International Human Genome Sequencing Consortium"],"citationCrossReferences":[{"database":"PubMed","id":"15496913"},{"database":"DOI","id":"10.1038/nature03001"}],"title":"Finishing the euchromatic sequence of the human genome.","publicationDate":"2004","journal":"Nature","firstPage":"931","lastPage":"945","volume":"431"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522746.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":4,"citation":{"id":"CI-20IM145UGQVF7","citationType":"submission","authoringGroup":["Ensembl"],"publicationDate":"JAN-2026","submissionDatabase":"UniProtKB"},"referencePositions":["IDENTIFICATION"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000522746.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AC008655","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"Ensembl","id":"ENST00000852687.1","properties":[{"key":"ProteinId","value":"ENSP00000522746.1"},{"key":"GeneId","value":"ENSG00000131400.10"}]},{"database":"HGNC","id":"HGNC:13395","properties":[{"key":"GeneName","value":"NAPSA"}]},{"database":"OpenTargets","id":"ENSG00000131400","properties":[{"key":"Description","value":"-"}]},{"database":"GeneTree","id":"ENSGT00940000160179","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000005640","properties":[{"key":"Component","value":"Chromosome 19"}]},{"database":"FunFam","id":"2.40.70.10:FF:000048","properties":[{"key":"EntryName","value":"Napsin A aspartic peptidase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.40.70.10:FF:000066","properties":[{"key":"EntryName","value":"Napsin A aspartic peptidase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.70.10","properties":[{"key":"EntryName","value":"Acid Proteases"},{"key":"MatchStatus","value":"3"}]},{"database":"InterPro","id":"IPR001461","properties":[{"key":"EntryName","value":"Aspartic_peptidase_A1"}]},{"database":"InterPro","id":"IPR001969","properties":[{"key":"EntryName","value":"Aspartic_peptidase_AS"}]},{"database":"InterPro","id":"IPR033121","properties":[{"key":"EntryName","value":"PEPTIDASE_A1"}]},{"database":"InterPro","id":"IPR021109","properties":[{"key":"EntryName","value":"Peptidase_aspartic_dom_sf"}]},{"database":"PANTHER","id":"PTHR47966","properties":[{"key":"EntryName","value":"BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR47966:SF83","properties":[{"key":"EntryName","value":"NAPSIN-A"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00026","properties":[{"key":"EntryName","value":"Asp"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00792","properties":[{"key":"EntryName","value":"PEPSIN"}]},{"database":"SUPFAM","id":"SSF50630","properties":[{"key":"EntryName","value":"Acid proteases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00141","properties":[{"key":"EntryName","value":"ASP_PROTEASE"},{"key":"MatchStatus","value":"2"}]},{"database":"PROSITE","id":"PS51767","properties":[{"key":"EntryName","value":"PEPTIDASE_A1"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSPPPLLQPLLLLLPLLNVEPSGATLIRIPLHRVQPGRRILNLLRGWREPAELPKLGAPSPGDKPIFVPLSNYRDYFGEIGLGTPPQNFTVAFDTGSSNLWVPSRRCHFFSVPCWLHHRFDPKASSSFQANGTKFAIQYGTGRVDGILSEDKLTIGGIKGASVIFGEALWEPSLVFAFAHFDGILGLGFPILSVEGVRPPMDVLVEQGLLDKPVFSFYLNRDPEEPDGGELVLGGSDPAHYIPPLTFVPVTVPAYWQIHMERVKVGPGLTLCAKGCAAILDTGTSLITGPTEEIRALHAAIGGIPLLAGEYIILCSEIPKLPAVSFLLGGVWFNLTAHDYVIQTTRNGVRLCLSGFQALDVPPPAGPFWILGDVFLGTYVAVFDRGDMKSSARVGLARARTRGADLGWGETAQAQFPG","length":418,"molWeight":45159,"crc64":"8031B84D27F828D4","md5":"807116A07D42AF5DD19A926351A55F58"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Signal":1,"Chain":1,"Domain":1,"Active site":2},"uniParcId":"UPI00403217B5"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0ACI8VGB3","uniProtkbId":"A0ACI8VGB3_HUMAN","entryAudit":{"firstPublicDate":"2026-06-10","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2026-06-10","entryVersion":2,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Homo sapiens","commonName":"Human","taxonId":9606,"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000638000.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}],"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Primates","Haplorrhini","Catarrhini","Hominidae","Homo"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}],"value":"Pepsin A"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}],"value":"3.4.23.1"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000638000.1"}],"value":"NAPSA"}}],"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.","ecNumber":"3.4.23.1","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007447"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000454"}],"value":"Belongs to the peptidase A1 family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":24,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}]},{"type":"Chain","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":379,"modifier":"EXACT"}},"description":"Pepsin A","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}],"featureId":"PRO_5051544692"},{"type":"Domain","location":{"start":{"value":78,"modifier":"EXACT"},"end":{"value":358,"modifier":"EXACT"}},"description":"Peptidase A1","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51767"}]},{"type":"Active site","location":{"start":{"value":96,"modifier":"EXACT"},"end":{"value":96,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}]},{"type":"Active site","location":{"start":{"value":242,"modifier":"EXACT"},"end":{"value":242,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022750"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"id":"KW-0064","category":"Molecular function","name":"Aspartyl protease"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023157"}],"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023180"}],"id":"KW-0325","category":"PTM","name":"Glycoprotein"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022801"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022670"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"id":"KW-0645","category":"Molecular function","name":"Protease"},{"evidences":[{"evidenceCode":"ECO:0007829","source":"PeptideAtlas","id":"A0ACI8VGB3"},{"evidenceCode":"ECO:0007829","source":"ProteomicsDB","id":"A0ACI8VGB3"}],"id":"KW-1267","category":"Technical term","name":"Proteomics identification"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022729"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}],"id":"KW-0732","category":"Domain","name":"Signal"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023145"}],"id":"KW-0865","category":"PTM","name":"Zymogen"}],"references":[{"referenceNumber":1,"citation":{"id":"11237011","citationType":"journal article","authoringGroup":["International Human Genome Sequencing Consortium"],"authors":["Lander E.S.","Linton L.M.","Birren B.","Nusbaum C.","Zody M.C.","Baldwin J.","Devon K.","Dewar K.","Doyle M.","FitzHugh W.","Funke R.","Gage D.","Harris K.","Heaford A.","Howland J.","Kann L.","Lehoczky J.","LeVine R.","McEwan P.","McKernan K.","Meldrim J.","Mesirov J.P.","Miranda C.","Morris W.","Naylor J.","Raymond C.","Rosetti M.","Santos R.","Sheridan A.","Sougnez C.","Stange-Thomann N.","Stojanovic N.","Subramanian A.","Wyman D.","Rogers J.","Sulston J.","Ainscough R.","Beck S.","Bentley D.","Burton J.","Clee C.","Carter N.","Coulson A.","Deadman R.","Deloukas P.","Dunham A.","Dunham I.","Durbin R.","French L.","Grafham D.","Gregory S.","Hubbard T.","Humphray S.","Hunt A.","Jones M.","Lloyd C.","McMurray A.","Matthews L.","Mercer S.","Milne S.","Mullikin J.C.","Mungall A.","Plumb R.","Ross M.","Shownkeen R.","Sims S.","Waterston R.H.","Wilson R.K.","Hillier L.W.","McPherson J.D.","Marra M.A.","Mardis E.R.","Fulton L.A.","Chinwalla A.T.","Pepin K.H.","Gish W.R.","Chissoe S.L.","Wendl M.C.","Delehaunty K.D.","Miner T.L.","Delehaunty A.","Kramer J.B.","Cook L.L.","Fulton R.S.","Johnson D.L.","Minx P.J.","Clifton S.W.","Hawkins T.","Branscomb E.","Predki P.","Richardson P.","Wenning S.","Slezak T.","Doggett N.","Cheng J.F.","Olsen A.","Lucas S.","Elkin C.","Uberbacher E.","Frazier M.","Gibbs R.A.","Muzny D.M.","Scherer S.E.","Bouck J.B.","Sodergren E.J.","Worley K.C.","Rives C.M.","Gorrell J.H.","Metzker M.L.","Naylor S.L.","Kucherlapati R.S.","Nelson D.L.","Weinstock G.M.","Sakaki Y.","Fujiyama A.","Hattori M.","Yada T.","Toyoda A.","Itoh T.","Kawagoe C.","Watanabe H.","Totoki Y.","Taylor T.","Weissenbach J.","Heilig R.","Saurin W.","Artiguenave F.","Brottier P.","Bruls T.","Pelletier E.","Robert C.","Wincker P.","Smith D.R.","Doucette-Stamm L.","Rubenfield M.","Weinstock K.","Lee H.M.","Dubois J.","Rosenthal A.","Platzer M.","Nyakatura G.","Taudien S.","Rump A.","Yang H.","Yu J.","Wang J.","Huang G.","Gu J.","Hood L.","Rowen L.","Madan A.","Qin S.","Davis R.W.","Federspiel N.A.","Abola A.P.","Proctor M.J.","Myers R.M.","Schmutz J.","Dickson M.","Grimwood J.","Cox D.R.","Olson M.V.","Kaul R.","Raymond C.","Shimizu N.","Kawasaki K.","Minoshima S.","Evans G.A.","Athanasiou M.","Schultz R.","Roe B.A.","Chen F.","Pan H.","Ramser J.","Lehrach H.","Reinhardt R.","McCombie W.R.","de la Bastide M.","Dedhia N.","Blocker H.","Hornischer K.","Nordsiek G.","Agarwala R.","Aravind L.","Bailey J.A.","Bateman A.","Batzoglou S.","Birney E.","Bork P.","Brown D.G.","Burge C.B.","Cerutti L.","Chen H.C.","Church D.","Clamp M.","Copley R.R.","Doerks T.","Eddy S.R.","Eichler E.E.","Furey T.S.","Galagan J.","Gilbert J.G.","Harmon C.","Hayashizaki Y.","Haussler D.","Hermjakob H.","Hokamp K.","Jang W.","Johnson L.S.","Jones T.A.","Kasif S.","Kaspryzk A.","Kennedy S.","Kent W.J.","Kitts P.","Koonin E.V.","Korf I.","Kulp D.","Lancet D.","Lowe T.M.","McLysaght A.","Mikkelsen T.","Moran J.V.","Mulder N.","Pollara V.J.","Ponting C.P.","Schuler G.","Schultz J.","Slater G.","Smit A.F.","Stupka E.","Szustakowski J.","Thierry-Mieg D.","Thierry-Mieg J.","Wagner L.","Wallis J.","Wheeler R.","Williams A.","Wolf Y.I.","Wolfe K.H.","Yang S.P.","Yeh R.F.","Collins F.","Guyer M.S.","Peterson J.","Felsenfeld A.","Wetterstrand K.A.","Patrinos A.","Morgan M.J.","de Jong P.","Catanese J.J.","Osoegawa K.","Shizuya H.","Choi S.","Chen Y.J."],"citationCrossReferences":[{"database":"PubMed","id":"11237011"},{"database":"DOI","id":"10.1038/35057062"}],"title":"Initial sequencing and analysis of the human genome.","publicationDate":"2001","journal":"Nature","firstPage":"860","lastPage":"921","volume":"409"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000638000.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":2,"citation":{"id":"15057824","citationType":"journal article","authors":["Grimwood J.","Gordon L.A.","Olsen A.","Terry A.","Schmutz J.","Lamerdin J.","Hellsten U.","Goodstein D.","Couronne O.","Tran-Gyamfi M.","Aerts A.","Altherr M.","Ashworth L.","Bajorek E.","Black S.","Branscomb E.","Caenepeel S.","Carrano A.","Caoile C.","Chan Y.M.","Christensen M.","Cleland C.A.","Copeland A.","Dalin E.","Dehal P.","Denys M.","Detter J.C.","Escobar J.","Flowers D.","Fotopulos D.","Garcia C.","Georgescu A.M.","Glavina T.","Gomez M.","Gonzales E.","Groza M.","Hammon N.","Hawkins T.","Haydu L.","Ho I.","Huang W.","Israni S.","Jett J.","Kadner K.","Kimball H.","Kobayashi A.","Larionov V.","Leem S.H.","Lopez F.","Lou Y.","Lowry S.","Malfatti S.","Martinez D.","McCready P.","Medina C.","Morgan J.","Nelson K.","Nolan M.","Ovcharenko I.","Pitluck S.","Pollard M.","Popkie A.P.","Predki P.","Quan G.","Ramirez L.","Rash S.","Retterer J.","Rodriguez A.","Rogers S.","Salamov A.","Salazar A.","She X.","Smith D.","Slezak T.","Solovyev V.","Thayer N.","Tice H.","Tsai M.","Ustaszewska A.","Vo N.","Wagner M.","Wheeler J.","Wu K.","Xie G.","Yang J.","Dubchak I.","Furey T.S.","DeJong P.","Dickson M.","Gordon D.","Eichler E.E.","Pennacchio L.A.","Richardson P.","Stubbs L.","Rokhsar D.S.","Myers R.M.","Rubin E.M.","Lucas S.M."],"citationCrossReferences":[{"database":"PubMed","id":"15057824"},{"database":"DOI","id":"10.1038/nature02399"}],"title":"The DNA sequence and biology of human chromosome 19.","publicationDate":"2004","journal":"Nature","firstPage":"529","lastPage":"535","volume":"428"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000638000.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":3,"citation":{"id":"15496913","citationType":"journal article","authoringGroup":["International Human Genome Sequencing Consortium"],"citationCrossReferences":[{"database":"PubMed","id":"15496913"},{"database":"DOI","id":"10.1038/nature03001"}],"title":"Finishing the euchromatic sequence of the human genome.","publicationDate":"2004","journal":"Nature","firstPage":"931","lastPage":"945","volume":"431"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000638000.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":4,"citation":{"id":"CI-20IM145UGQVF7","citationType":"submission","authoringGroup":["Ensembl"],"publicationDate":"JAN-2026","submissionDatabase":"UniProtKB"},"referencePositions":["IDENTIFICATION"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000638000.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AC008655","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"Ensembl","id":"ENST00000967941.1","properties":[{"key":"ProteinId","value":"ENSP00000638000.1"},{"key":"GeneId","value":"ENSG00000131400.10"}]},{"database":"HGNC","id":"HGNC:13395","properties":[{"key":"GeneName","value":"NAPSA"}]},{"database":"OpenTargets","id":"ENSG00000131400","properties":[{"key":"Description","value":"-"}]},{"database":"GeneTree","id":"ENSGT00940000160179","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000005640","properties":[{"key":"Component","value":"Chromosome 19"}]},{"database":"FunFam","id":"2.40.70.10:FF:000008","properties":[{"key":"EntryName","value":"Cathepsin D"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.40.70.10:FF:000066","properties":[{"key":"EntryName","value":"Napsin A aspartic peptidase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.70.10","properties":[{"key":"EntryName","value":"Acid Proteases"},{"key":"MatchStatus","value":"2"}]},{"database":"InterPro","id":"IPR001461","properties":[{"key":"EntryName","value":"Aspartic_peptidase_A1"}]},{"database":"InterPro","id":"IPR001969","properties":[{"key":"EntryName","value":"Aspartic_peptidase_AS"}]},{"database":"InterPro","id":"IPR033121","properties":[{"key":"EntryName","value":"PEPTIDASE_A1"}]},{"database":"InterPro","id":"IPR021109","properties":[{"key":"EntryName","value":"Peptidase_aspartic_dom_sf"}]},{"database":"PANTHER","id":"PTHR47966","properties":[{"key":"EntryName","value":"BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR47966:SF83","properties":[{"key":"EntryName","value":"NAPSIN-A"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00026","properties":[{"key":"EntryName","value":"Asp"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00792","properties":[{"key":"EntryName","value":"PEPSIN"}]},{"database":"SUPFAM","id":"SSF50630","properties":[{"key":"EntryName","value":"Acid proteases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00141","properties":[{"key":"EntryName","value":"ASP_PROTEASE"},{"key":"MatchStatus","value":"2"}]},{"database":"PROSITE","id":"PS51767","properties":[{"key":"EntryName","value":"PEPTIDASE_A1"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSPPPLLQPLLLLLPLLNVEPSGATLIRIPLHRVQPGRRILNLLRGWREPAELPKLGAPSPGDKPIFVPLSNYRDVQYFGEIGLGTPPQNFTVAFDTGSSNLWVPSRRCHFFSVPCWLHHRFDPKASSSFQANGTKFAIQYGTGRVDGILSEDKLTIGGIKGASVIFGEALWEPSLVFAFAHFDGILGLGFPILSVEGVRPPMDVLVEQGLLDKPVFSFYLNSVKVGPGLTLCAKGCAAILDTGTSLITGPTEEIRALHAAIGGIPLLAGEYIILCSEIPKLPAVSFLLGGVWFNLTAHDYVIQTTRNGVRLCLSGFQALDVPPPAGPFWILGDVFLGTYVAVFDRGDMKSSARVGLARARTRGADLGWGETAQAQFPG","length":379,"molWeight":40804,"crc64":"2B54250ACEBEDAE9","md5":"6AA1D6893CBA01CBF1D8B13AB3C2BC6B"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Signal":1,"Chain":1,"Domain":1,"Active site":2},"uniParcId":"UPI0003E69497"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0ACI8VJQ4","uniProtkbId":"A0ACI8VJQ4_HUMAN","entryAudit":{"firstPublicDate":"2026-06-10","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2026-06-10","entryVersion":2,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Homo sapiens","commonName":"Human","taxonId":9606,"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000638001.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}],"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Primates","Haplorrhini","Catarrhini","Hominidae","Homo"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}],"value":"Pepsin A"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}],"value":"3.4.23.1"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000638001.1"}],"value":"NAPSA"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00055304"}],"value":"May be involved in processing of pneumocyte surfactant precursors"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.","ecNumber":"3.4.23.1","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00004613"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00007447"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"},{"evidenceCode":"ECO:0000256","source":"RuleBase","id":"RU000454"}],"value":"Belongs to the peptidase A1 family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"value":"Lacks conserved residue(s) required for the propagation of feature annotation"}],"commentType":"CAUTION"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":24,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}]},{"type":"Chain","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":412,"modifier":"EXACT"}},"description":"Pepsin A","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}],"featureId":"PRO_5051352567"},{"type":"Domain","location":{"start":{"value":70,"modifier":"EXACT"},"end":{"value":391,"modifier":"EXACT"}},"description":"Peptidase A1","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS51767"}]},{"type":"Active site","location":{"start":{"value":88,"modifier":"EXACT"},"end":{"value":88,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}]},{"type":"Active site","location":{"start":{"value":275,"modifier":"EXACT"},"end":{"value":275,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU10094"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022750"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"id":"KW-0064","category":"Molecular function","name":"Aspartyl protease"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023157"}],"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023180"}],"id":"KW-0325","category":"PTM","name":"Glycoprotein"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022801"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022670"},{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01103"}],"id":"KW-0645","category":"Molecular function","name":"Protease"},{"evidences":[{"evidenceCode":"ECO:0007829","source":"PeptideAtlas","id":"A0ACI8VJQ4"},{"evidenceCode":"ECO:0007829","source":"ProteomicsDB","id":"A0ACI8VJQ4"}],"id":"KW-1267","category":"Technical term","name":"Proteomics identification"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022525"}],"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022729"},{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}],"id":"KW-0732","category":"Domain","name":"Signal"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00023145"}],"id":"KW-0865","category":"PTM","name":"Zymogen"}],"references":[{"referenceNumber":1,"citation":{"id":"11237011","citationType":"journal article","authoringGroup":["International Human Genome Sequencing Consortium"],"authors":["Lander E.S.","Linton L.M.","Birren B.","Nusbaum C.","Zody M.C.","Baldwin J.","Devon K.","Dewar K.","Doyle M.","FitzHugh W.","Funke R.","Gage D.","Harris K.","Heaford A.","Howland J.","Kann L.","Lehoczky J.","LeVine R.","McEwan P.","McKernan K.","Meldrim J.","Mesirov J.P.","Miranda C.","Morris W.","Naylor J.","Raymond C.","Rosetti M.","Santos R.","Sheridan A.","Sougnez C.","Stange-Thomann N.","Stojanovic N.","Subramanian A.","Wyman D.","Rogers J.","Sulston J.","Ainscough R.","Beck S.","Bentley D.","Burton J.","Clee C.","Carter N.","Coulson A.","Deadman R.","Deloukas P.","Dunham A.","Dunham I.","Durbin R.","French L.","Grafham D.","Gregory S.","Hubbard T.","Humphray S.","Hunt A.","Jones M.","Lloyd C.","McMurray A.","Matthews L.","Mercer S.","Milne S.","Mullikin J.C.","Mungall A.","Plumb R.","Ross M.","Shownkeen R.","Sims S.","Waterston R.H.","Wilson R.K.","Hillier L.W.","McPherson J.D.","Marra M.A.","Mardis E.R.","Fulton L.A.","Chinwalla A.T.","Pepin K.H.","Gish W.R.","Chissoe S.L.","Wendl M.C.","Delehaunty K.D.","Miner T.L.","Delehaunty A.","Kramer J.B.","Cook L.L.","Fulton R.S.","Johnson D.L.","Minx P.J.","Clifton S.W.","Hawkins T.","Branscomb E.","Predki P.","Richardson P.","Wenning S.","Slezak T.","Doggett N.","Cheng J.F.","Olsen A.","Lucas S.","Elkin C.","Uberbacher E.","Frazier M.","Gibbs R.A.","Muzny D.M.","Scherer S.E.","Bouck J.B.","Sodergren E.J.","Worley K.C.","Rives C.M.","Gorrell J.H.","Metzker M.L.","Naylor S.L.","Kucherlapati R.S.","Nelson D.L.","Weinstock G.M.","Sakaki Y.","Fujiyama A.","Hattori M.","Yada T.","Toyoda A.","Itoh T.","Kawagoe C.","Watanabe H.","Totoki Y.","Taylor T.","Weissenbach J.","Heilig R.","Saurin W.","Artiguenave F.","Brottier P.","Bruls T.","Pelletier E.","Robert C.","Wincker P.","Smith D.R.","Doucette-Stamm L.","Rubenfield M.","Weinstock K.","Lee H.M.","Dubois J.","Rosenthal A.","Platzer M.","Nyakatura G.","Taudien S.","Rump A.","Yang H.","Yu J.","Wang J.","Huang G.","Gu J.","Hood L.","Rowen L.","Madan A.","Qin S.","Davis R.W.","Federspiel N.A.","Abola A.P.","Proctor M.J.","Myers R.M.","Schmutz J.","Dickson M.","Grimwood J.","Cox D.R.","Olson M.V.","Kaul R.","Raymond C.","Shimizu N.","Kawasaki K.","Minoshima S.","Evans G.A.","Athanasiou M.","Schultz R.","Roe B.A.","Chen F.","Pan H.","Ramser J.","Lehrach H.","Reinhardt R.","McCombie W.R.","de la Bastide M.","Dedhia N.","Blocker H.","Hornischer K.","Nordsiek G.","Agarwala R.","Aravind L.","Bailey J.A.","Bateman A.","Batzoglou S.","Birney E.","Bork P.","Brown D.G.","Burge C.B.","Cerutti L.","Chen H.C.","Church D.","Clamp M.","Copley R.R.","Doerks T.","Eddy S.R.","Eichler E.E.","Furey T.S.","Galagan J.","Gilbert J.G.","Harmon C.","Hayashizaki Y.","Haussler D.","Hermjakob H.","Hokamp K.","Jang W.","Johnson L.S.","Jones T.A.","Kasif S.","Kaspryzk A.","Kennedy S.","Kent W.J.","Kitts P.","Koonin E.V.","Korf I.","Kulp D.","Lancet D.","Lowe T.M.","McLysaght A.","Mikkelsen T.","Moran J.V.","Mulder N.","Pollara V.J.","Ponting C.P.","Schuler G.","Schultz J.","Slater G.","Smit A.F.","Stupka E.","Szustakowski J.","Thierry-Mieg D.","Thierry-Mieg J.","Wagner L.","Wallis J.","Wheeler R.","Williams A.","Wolf Y.I.","Wolfe K.H.","Yang S.P.","Yeh R.F.","Collins F.","Guyer M.S.","Peterson J.","Felsenfeld A.","Wetterstrand K.A.","Patrinos A.","Morgan M.J.","de Jong P.","Catanese J.J.","Osoegawa K.","Shizuya H.","Choi S.","Chen Y.J."],"citationCrossReferences":[{"database":"PubMed","id":"11237011"},{"database":"DOI","id":"10.1038/35057062"}],"title":"Initial sequencing and analysis of the human genome.","publicationDate":"2001","journal":"Nature","firstPage":"860","lastPage":"921","volume":"409"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000638001.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":2,"citation":{"id":"15057824","citationType":"journal article","authors":["Grimwood J.","Gordon L.A.","Olsen A.","Terry A.","Schmutz J.","Lamerdin J.","Hellsten U.","Goodstein D.","Couronne O.","Tran-Gyamfi M.","Aerts A.","Altherr M.","Ashworth L.","Bajorek E.","Black S.","Branscomb E.","Caenepeel S.","Carrano A.","Caoile C.","Chan Y.M.","Christensen M.","Cleland C.A.","Copeland A.","Dalin E.","Dehal P.","Denys M.","Detter J.C.","Escobar J.","Flowers D.","Fotopulos D.","Garcia C.","Georgescu A.M.","Glavina T.","Gomez M.","Gonzales E.","Groza M.","Hammon N.","Hawkins T.","Haydu L.","Ho I.","Huang W.","Israni S.","Jett J.","Kadner K.","Kimball H.","Kobayashi A.","Larionov V.","Leem S.H.","Lopez F.","Lou Y.","Lowry S.","Malfatti S.","Martinez D.","McCready P.","Medina C.","Morgan J.","Nelson K.","Nolan M.","Ovcharenko I.","Pitluck S.","Pollard M.","Popkie A.P.","Predki P.","Quan G.","Ramirez L.","Rash S.","Retterer J.","Rodriguez A.","Rogers S.","Salamov A.","Salazar A.","She X.","Smith D.","Slezak T.","Solovyev V.","Thayer N.","Tice H.","Tsai M.","Ustaszewska A.","Vo N.","Wagner M.","Wheeler J.","Wu K.","Xie G.","Yang J.","Dubchak I.","Furey T.S.","DeJong P.","Dickson M.","Gordon D.","Eichler E.E.","Pennacchio L.A.","Richardson P.","Stubbs L.","Rokhsar D.S.","Myers R.M.","Rubin E.M.","Lucas S.M."],"citationCrossReferences":[{"database":"PubMed","id":"15057824"},{"database":"DOI","id":"10.1038/nature02399"}],"title":"The DNA sequence and biology of human chromosome 19.","publicationDate":"2004","journal":"Nature","firstPage":"529","lastPage":"535","volume":"428"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000638001.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":3,"citation":{"id":"15496913","citationType":"journal article","authoringGroup":["International Human Genome Sequencing Consortium"],"citationCrossReferences":[{"database":"PubMed","id":"15496913"},{"database":"DOI","id":"10.1038/nature03001"}],"title":"Finishing the euchromatic sequence of the human genome.","publicationDate":"2004","journal":"Nature","firstPage":"931","lastPage":"945","volume":"431"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000638001.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":4,"citation":{"id":"CI-20IM145UGQVF7","citationType":"submission","authoringGroup":["Ensembl"],"publicationDate":"JAN-2026","submissionDatabase":"UniProtKB"},"referencePositions":["IDENTIFICATION"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000638001.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AC008655","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"Ensembl","id":"ENST00000967942.1","properties":[{"key":"ProteinId","value":"ENSP00000638001.1"},{"key":"GeneId","value":"ENSG00000131400.10"}]},{"database":"HGNC","id":"HGNC:13395","properties":[{"key":"GeneName","value":"NAPSA"}]},{"database":"OpenTargets","id":"ENSG00000131400","properties":[{"key":"Description","value":"-"}]},{"database":"GeneTree","id":"ENSGT00940000160179","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000005640","properties":[{"key":"Component","value":"Chromosome 19"}]},{"database":"FunFam","id":"2.40.70.10:FF:000048","properties":[{"key":"EntryName","value":"Napsin A aspartic peptidase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.40.70.10:FF:000066","properties":[{"key":"EntryName","value":"Napsin A aspartic peptidase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.70.10","properties":[{"key":"EntryName","value":"Acid Proteases"},{"key":"MatchStatus","value":"3"}]},{"database":"InterPro","id":"IPR001461","properties":[{"key":"EntryName","value":"Aspartic_peptidase_A1"}]},{"database":"InterPro","id":"IPR001969","properties":[{"key":"EntryName","value":"Aspartic_peptidase_AS"}]},{"database":"InterPro","id":"IPR033121","properties":[{"key":"EntryName","value":"PEPTIDASE_A1"}]},{"database":"InterPro","id":"IPR021109","properties":[{"key":"EntryName","value":"Peptidase_aspartic_dom_sf"}]},{"database":"PANTHER","id":"PTHR47966","properties":[{"key":"EntryName","value":"BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR47966:SF83","properties":[{"key":"EntryName","value":"NAPSIN-A"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00026","properties":[{"key":"EntryName","value":"Asp"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00792","properties":[{"key":"EntryName","value":"PEPSIN"}]},{"database":"SUPFAM","id":"SSF50630","properties":[{"key":"EntryName","value":"Acid proteases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00141","properties":[{"key":"EntryName","value":"ASP_PROTEASE"},{"key":"MatchStatus","value":"2"}]},{"database":"PROSITE","id":"PS51767","properties":[{"key":"EntryName","value":"PEPTIDASE_A1"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSPPPLLQPLLLLLPLLNVEPSGATLIRIPLHRVQPGRRILNLLRGWREPAELPKLGAPSPGDKPIFVQYFGEIGLGTPPQNFTVAFDTGSSNLWVPSRRCHFFSVPCWLHHRFDPKASSSFQANGTKFAIQYGTGRVDGILSEDKLTIGGIKGASVIFGEALWEPSLVFAFAHFDGILGLGFPILSVEGVRPPMDVLVEQGLLDKPVFSFYLNRDPEEPDGGELVLGGSDPAHYIPPLTFVPVTVPAYWQIHMERVKVGPGLTLCAKGCAAILDTGTSLITGPTEEIRALHAAIGGIPLLAGEYIILCSEIPKLPAVSFLLGGVWFNLTAHDYVIQTTRNGVRLCLSGFQALDVPPPAGPFWILGDVFLGTYVAVFDRGDMKSSARVGLARARTRGADLGWGETAQAQFPG","length":412,"molWeight":44441,"crc64":"989739DB0C29C4F6","md5":"1EF1E160657146BBE70B4A8FE5AED7A6"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Signal":1,"Chain":1,"Domain":1,"Active site":2},"uniParcId":"UPI004031E6C9"}},{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A0B4J2A8","uniProtkbId":"A0A0B4J2A8_HUMAN","entryAudit":{"firstPublicDate":"2015-03-04","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2015-03-04","entryVersion":57,"sequenceVersion":1},"annotationScore":1.0,"organism":{"scientificName":"Homo sapiens","commonName":"Human","taxonId":9606,"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000469763.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}],"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Primates","Haplorrhini","Catarrhini","Hominidae","Homo"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"submissionNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000469763.1"}],"value":"Napsin A aspartic peptidase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000469763.1"}],"value":"NAPSA"}}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":24,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}]},{"type":"Chain","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":104,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}],"featureId":"PRO_5002092619"},{"type":"Region","location":{"start":{"value":84,"modifier":"EXACT"},"end":{"value":104,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0007829","source":"PeptideAtlas","id":"A0A0B4J2A8"},{"evidenceCode":"ECO:0007829","source":"ProteomicsDB","id":"A0A0B4J2A8"}],"id":"KW-1267","category":"Technical term","name":"Proteomics identification"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"SignalP"}],"id":"KW-0732","category":"Domain","name":"Signal"}],"references":[{"referenceNumber":1,"citation":{"id":"11237011","citationType":"journal article","authoringGroup":["International Human Genome Sequencing Consortium"],"authors":["Lander E.S.","Linton L.M.","Birren B.","Nusbaum C.","Zody M.C.","Baldwin J.","Devon K.","Dewar K.","Doyle M.","FitzHugh W.","Funke R.","Gage D.","Harris K.","Heaford A.","Howland J.","Kann L.","Lehoczky J.","LeVine R.","McEwan P.","McKernan K.","Meldrim J.","Mesirov J.P.","Miranda C.","Morris W.","Naylor J.","Raymond C.","Rosetti M.","Santos R.","Sheridan A.","Sougnez C.","Stange-Thomann N.","Stojanovic N.","Subramanian A.","Wyman D.","Rogers J.","Sulston J.","Ainscough R.","Beck S.","Bentley D.","Burton J.","Clee C.","Carter N.","Coulson A.","Deadman R.","Deloukas P.","Dunham A.","Dunham I.","Durbin R.","French L.","Grafham D.","Gregory S.","Hubbard T.","Humphray S.","Hunt A.","Jones M.","Lloyd C.","McMurray A.","Matthews L.","Mercer S.","Milne S.","Mullikin J.C.","Mungall A.","Plumb R.","Ross M.","Shownkeen R.","Sims S.","Waterston R.H.","Wilson R.K.","Hillier L.W.","McPherson J.D.","Marra M.A.","Mardis E.R.","Fulton L.A.","Chinwalla A.T.","Pepin K.H.","Gish W.R.","Chissoe S.L.","Wendl M.C.","Delehaunty K.D.","Miner T.L.","Delehaunty A.","Kramer J.B.","Cook L.L.","Fulton R.S.","Johnson D.L.","Minx P.J.","Clifton S.W.","Hawkins T.","Branscomb E.","Predki P.","Richardson P.","Wenning S.","Slezak T.","Doggett N.","Cheng J.F.","Olsen A.","Lucas S.","Elkin C.","Uberbacher E.","Frazier M.","Gibbs R.A.","Muzny D.M.","Scherer S.E.","Bouck J.B.","Sodergren E.J.","Worley K.C.","Rives C.M.","Gorrell J.H.","Metzker M.L.","Naylor S.L.","Kucherlapati R.S.","Nelson D.L.","Weinstock G.M.","Sakaki Y.","Fujiyama A.","Hattori M.","Yada T.","Toyoda A.","Itoh T.","Kawagoe C.","Watanabe H.","Totoki Y.","Taylor T.","Weissenbach J.","Heilig R.","Saurin W.","Artiguenave F.","Brottier P.","Bruls T.","Pelletier E.","Robert C.","Wincker P.","Smith D.R.","Doucette-Stamm L.","Rubenfield M.","Weinstock K.","Lee H.M.","Dubois J.","Rosenthal A.","Platzer M.","Nyakatura G.","Taudien S.","Rump A.","Yang H.","Yu J.","Wang J.","Huang G.","Gu J.","Hood L.","Rowen L.","Madan A.","Qin S.","Davis R.W.","Federspiel N.A.","Abola A.P.","Proctor M.J.","Myers R.M.","Schmutz J.","Dickson M.","Grimwood J.","Cox D.R.","Olson M.V.","Kaul R.","Raymond C.","Shimizu N.","Kawasaki K.","Minoshima S.","Evans G.A.","Athanasiou M.","Schultz R.","Roe B.A.","Chen F.","Pan H.","Ramser J.","Lehrach H.","Reinhardt R.","McCombie W.R.","de la Bastide M.","Dedhia N.","Blocker H.","Hornischer K.","Nordsiek G.","Agarwala R.","Aravind L.","Bailey J.A.","Bateman A.","Batzoglou S.","Birney E.","Bork P.","Brown D.G.","Burge C.B.","Cerutti L.","Chen H.C.","Church D.","Clamp M.","Copley R.R.","Doerks T.","Eddy S.R.","Eichler E.E.","Furey T.S.","Galagan J.","Gilbert J.G.","Harmon C.","Hayashizaki Y.","Haussler D.","Hermjakob H.","Hokamp K.","Jang W.","Johnson L.S.","Jones T.A.","Kasif S.","Kaspryzk A.","Kennedy S.","Kent W.J.","Kitts P.","Koonin E.V.","Korf I.","Kulp D.","Lancet D.","Lowe T.M.","McLysaght A.","Mikkelsen T.","Moran J.V.","Mulder N.","Pollara V.J.","Ponting C.P.","Schuler G.","Schultz J.","Slater G.","Smit A.F.","Stupka E.","Szustakowski J.","Thierry-Mieg D.","Thierry-Mieg J.","Wagner L.","Wallis J.","Wheeler R.","Williams A.","Wolf Y.I.","Wolfe K.H.","Yang S.P.","Yeh R.F.","Collins F.","Guyer M.S.","Peterson J.","Felsenfeld A.","Wetterstrand K.A.","Patrinos A.","Morgan M.J.","de Jong P.","Catanese J.J.","Osoegawa K.","Shizuya H.","Choi S.","Chen Y.J."],"citationCrossReferences":[{"database":"PubMed","id":"11237011"},{"database":"DOI","id":"10.1038/35057062"}],"title":"Initial sequencing and analysis of the human genome.","publicationDate":"2001","journal":"Nature","firstPage":"860","lastPage":"921","volume":"409"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000469763.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":2,"citation":{"id":"15057824","citationType":"journal article","authors":["Grimwood J.","Gordon L.A.","Olsen A.","Terry A.","Schmutz J.","Lamerdin J.","Hellsten U.","Goodstein D.","Couronne O.","Tran-Gyamfi M.","Aerts A.","Altherr M.","Ashworth L.","Bajorek E.","Black S.","Branscomb E.","Caenepeel S.","Carrano A.","Caoile C.","Chan Y.M.","Christensen M.","Cleland C.A.","Copeland A.","Dalin E.","Dehal P.","Denys M.","Detter J.C.","Escobar J.","Flowers D.","Fotopulos D.","Garcia C.","Georgescu A.M.","Glavina T.","Gomez M.","Gonzales E.","Groza M.","Hammon N.","Hawkins T.","Haydu L.","Ho I.","Huang W.","Israni S.","Jett J.","Kadner K.","Kimball H.","Kobayashi A.","Larionov V.","Leem S.H.","Lopez F.","Lou Y.","Lowry S.","Malfatti S.","Martinez D.","McCready P.","Medina C.","Morgan J.","Nelson K.","Nolan M.","Ovcharenko I.","Pitluck S.","Pollard M.","Popkie A.P.","Predki P.","Quan G.","Ramirez L.","Rash S.","Retterer J.","Rodriguez A.","Rogers S.","Salamov A.","Salazar A.","She X.","Smith D.","Slezak T.","Solovyev V.","Thayer N.","Tice H.","Tsai M.","Ustaszewska A.","Vo N.","Wagner M.","Wheeler J.","Wu K.","Xie G.","Yang J.","Dubchak I.","Furey T.S.","DeJong P.","Dickson M.","Gordon D.","Eichler E.E.","Pennacchio L.A.","Richardson P.","Stubbs L.","Rokhsar D.S.","Myers R.M.","Rubin E.M.","Lucas S.M."],"citationCrossReferences":[{"database":"PubMed","id":"15057824"},{"database":"DOI","id":"10.1038/nature02399"}],"title":"The DNA sequence and biology of human chromosome 19.","publicationDate":"2004","journal":"Nature","firstPage":"529","lastPage":"535","volume":"428"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000469763.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":3,"citation":{"id":"15496913","citationType":"journal article","authoringGroup":["International Human Genome Sequencing Consortium"],"citationCrossReferences":[{"database":"PubMed","id":"15496913"},{"database":"DOI","id":"10.1038/nature03001"}],"title":"Finishing the euchromatic sequence of the human genome.","publicationDate":"2004","journal":"Nature","firstPage":"931","lastPage":"945","volume":"431"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000469763.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000005640"}]},{"referenceNumber":4,"citation":{"id":"CI-20IM145UGQVF7","citationType":"submission","authoringGroup":["Ensembl"],"publicationDate":"JAN-2026","submissionDatabase":"UniProtKB"},"referencePositions":["IDENTIFICATION"],"evidences":[{"evidenceCode":"ECO:0000313","source":"Ensembl","id":"ENSP00000469763.1"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AC008655","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A0B4J2A8","properties":[{"key":"Description","value":"-"}]},{"database":"MassIVE","id":"A0A0B4J2A8","properties":[{"key":"Description","value":"-"}]},{"database":"Ensembl","id":"ENST00000599233.1","properties":[{"key":"ProteinId","value":"ENSP00000469763.1"},{"key":"GeneId","value":"ENSG00000131400.10"}]},{"database":"HGNC","id":"HGNC:13395","properties":[{"key":"GeneName","value":"NAPSA"}]},{"database":"VEuPathDB","id":"HostDB:ENSG00000131400","properties":[{"key":"Description","value":"-"}]},{"database":"GeneTree","id":"ENSGT00940000160179","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_2249159_0_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"771136at2759","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000005640","properties":[{"key":"Component","value":"Chromosome 19"}]},{"database":"Bgee","id":"ENSG00000131400","properties":[{"key":"ExpressionPatterns","value":"Expressed in lower lobe of lung and 112 other cell types or tissues"}]},{"database":"ExpressionAtlas","id":"A0A0B4J2A8","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]}],"sequence":{"value":"MSPPPLLQPLLLLLPLLNVEPSGATLIRIPLHRVQPGRRILNLLRGWREPAELPKLGAPSPGDKPIFVPLSNYRDGYTTDLIPKPLAPSRPMGPSLPFNMELGG","length":104,"molWeight":11370,"crc64":"1524851C1D09F85F","md5":"1BB786F4FB968887047DA8F145748266"},"extraAttributes":{"countByFeatureType":{"Signal":1,"Chain":1,"Region":1},"uniParcId":"UPI00004C7D17"}}]}