{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9Y7S9","uniProtkbId":"AGLT3_SCHPO","entryAudit":{"firstPublicDate":"2002-11-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1999-11-01","entryVersion":169,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Schizosaccharomyces pombe (strain 972 / ATCC 24843)","commonName":"Fission yeast","taxonId":284812,"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Taphrinomycotina","Schizosaccharomycetes","Schizosaccharomycetales","Schizosaccharomycetaceae","Schizosaccharomyces"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"4-alpha-glucanotransferase aah3"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A2QTS4"}],"value":"2.4.1.25"}]},"flag":"Precursor"},"genes":[{"geneName":{"value":"aah3"},"orfNames":[{"value":"SPCC63.02c"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A2QTS4"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16751704"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"40668835"}],"value":"Transfers a segment of a (1->4)-alpha-D-glucan to an acceptor oligosaccharide; may exhibit activity on alpha(1,3)- and alpha(1,4)-linked oligosaccharides (By similarity). Contributes to cell wall polysaccharide biosynthesis and cell wall assembly (PubMed:16751704, PubMed:40668835)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan.","ecNumber":"2.4.1.25","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A2QTS4"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Ca(2+)","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0C1B3"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29108"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0C1B3"}],"value":"Binds 2 calcium ions per subunit. Calcium is inhibitory at high concentrations."}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"40668835"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255"}],"value":"Lipid-anchor, GPI-anchor","id":"SL-9902"},"orientation":{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"40668835"}],"value":"Extracellular side","id":"SL-9911"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16751704"}],"value":"N-glycosylated"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"40668835"}],"value":"Leads to abnormal morphology with pear-shaped (one end rounded) and stubby (shorter length and larger diameter) cells formed; double-knockout with aah1 leads to a defect in cell separation following cytokinesis and cells appear spherical, have an abnormal cell wall with a loss of alpha-1,3 glucan and exhibit a growth defect (PubMed:40668835). Sensitive to cold; double-knockout with aah1 leads to a growth defect at all temperatures (PubMed:40668835)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the glycosyl hydrolase 13 family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"16751704"}],"value":"Was originally thought to exhibit alpha-amylase activity, but this activity is not detectable in S.pombe cell lysates or culture media"}],"commentType":"CAUTION"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":21,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Chain","location":{"start":{"value":22,"modifier":"EXACT"},"end":{"value":538,"modifier":"EXACT"}},"description":"4-alpha-glucanotransferase aah3","featureId":"PRO_0000001358"},{"type":"Propeptide","location":{"start":{"value":539,"modifier":"EXACT"},"end":{"value":564,"modifier":"EXACT"}},"description":"Removed in mature form","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":"PRO_0000255453"},{"type":"Active site","location":{"start":{"value":229,"modifier":"EXACT"},"end":{"value":229,"modifier":"EXACT"}},"description":"Nucleophile","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"16751704"}]},{"type":"Active site","location":{"start":{"value":253,"modifier":"EXACT"},"end":{"value":253,"modifier":"EXACT"}},"description":"Proton donor","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"16751704"}]},{"type":"Binding site","location":{"start":{"value":56,"modifier":"EXACT"},"end":{"value":56,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0C1B3"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":105,"modifier":"EXACT"},"end":{"value":105,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0C1B3"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":143,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29108"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0C1B3"}],"ligand":{"name":"Ca(2+)","id":"ChEBI:CHEBI:29108","label":"1"}},{"type":"Binding site","location":{"start":{"value":198,"modifier":"EXACT"},"end":{"value":198,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29108"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P56271"}],"ligand":{"name":"Ca(2+)","id":"ChEBI:CHEBI:29108","label":"1"}},{"type":"Binding site","location":{"start":{"value":227,"modifier":"EXACT"},"end":{"value":227,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0C1B3"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":229,"modifier":"EXACT"},"end":{"value":229,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29108"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0C1B3"}],"ligand":{"name":"Ca(2+)","id":"ChEBI:CHEBI:29108","label":"2"}},{"type":"Binding site","location":{"start":{"value":232,"modifier":"EXACT"},"end":{"value":233,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0C1B3"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":253,"modifier":"EXACT"},"end":{"value":253,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29108"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0C1B3"}],"ligand":{"name":"Ca(2+)","id":"ChEBI:CHEBI:29108","label":"2"}},{"type":"Binding site","location":{"start":{"value":322,"modifier":"EXACT"},"end":{"value":322,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0C1B3"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":369,"modifier":"EXACT"},"end":{"value":369,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0C1B3"}],"ligand":{"name":"substrate"}},{"type":"Site","location":{"start":{"value":322,"modifier":"EXACT"},"end":{"value":322,"modifier":"EXACT"}},"description":"Transition state stabilizer","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0C1B3"}]},{"type":"Lipidation","location":{"start":{"value":538,"modifier":"EXACT"},"end":{"value":538,"modifier":"EXACT"}},"description":"GPI-anchor amidated serine","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Glycosylation","location":{"start":{"value":181,"modifier":"EXACT"},"end":{"value":181,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":235,"modifier":"EXACT"},"end":{"value":235,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":282,"modifier":"EXACT"},"end":{"value":282,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":305,"modifier":"EXACT"},"end":{"value":305,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":438,"modifier":"EXACT"},"end":{"value":438,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":447,"modifier":"EXACT"},"end":{"value":447,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":498,"modifier":"EXACT"},"end":{"value":498,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""},{"type":"Disulfide bond","location":{"start":{"value":51,"modifier":"EXACT"},"end":{"value":59,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P56271"}]},{"type":"Disulfide bond","location":{"start":{"value":172,"modifier":"EXACT"},"end":{"value":188,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P56271"}]},{"type":"Disulfide bond","location":{"start":{"value":263,"modifier":"EXACT"},"end":{"value":306,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P56271"}]},{"type":"Mutagenesis","location":{"start":{"value":229,"modifier":"EXACT"},"end":{"value":229,"modifier":"EXACT"}},"description":"No activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16751704"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":253,"modifier":"EXACT"},"end":{"value":253,"modifier":"EXACT"}},"description":"No activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16751704"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["A"]}}],"keywords":[{"id":"KW-0106","category":"Ligand","name":"Calcium"},{"id":"KW-0119","category":"Biological process","name":"Carbohydrate metabolism"},{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0961","category":"Biological process","name":"Cell wall biogenesis/degradation"},{"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"id":"KW-0325","category":"PTM","name":"Glycoprotein"},{"id":"KW-0326","category":"Molecular function","name":"Glycosidase"},{"id":"KW-0328","category":"Molecular function","name":"Glycosyltransferase"},{"id":"KW-0336","category":"PTM","name":"GPI-anchor"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0449","category":"PTM","name":"Lipoprotein"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0732","category":"Domain","name":"Signal"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"11859360","citationType":"journal article","authors":["Wood V.","Gwilliam R.","Rajandream M.A.","Lyne M.H.","Lyne R.","Stewart A.","Sgouros J.G.","Peat N.","Hayles J.","Baker S.G.","Basham D.","Bowman S.","Brooks K.","Brown D.","Brown S.","Chillingworth T.","Churcher C.M.","Collins M.","Connor R.","Cronin A.","Davis P.","Feltwell T.","Fraser A.","Gentles S.","Goble A.","Hamlin N.","Harris D.E.","Hidalgo J.","Hodgson G.","Holroyd S.","Hornsby T.","Howarth S.","Huckle E.J.","Hunt S.","Jagels K.","James K.D.","Jones L.","Jones M.","Leather S.","McDonald S.","McLean J.","Mooney P.","Moule S.","Mungall K.L.","Murphy L.D.","Niblett D.","Odell C.","Oliver K.","O'Neil S.","Pearson D.","Quail M.A.","Rabbinowitsch E.","Rutherford K.M.","Rutter S.","Saunders D.","Seeger K.","Sharp S.","Skelton J.","Simmonds M.N.","Squares R.","Squares S.","Stevens K.","Taylor K.","Taylor R.G.","Tivey A.","Walsh S.V.","Warren T.","Whitehead S.","Woodward J.R.","Volckaert G.","Aert R.","Robben J.","Grymonprez B.","Weltjens I.","Vanstreels E.","Rieger M.","Schaefer M.","Mueller-Auer S.","Gabel C.","Fuchs M.","Duesterhoeft A.","Fritzc C.","Holzer E.","Moestl D.","Hilbert H.","Borzym K.","Langer I.","Beck A.","Lehrach H.","Reinhardt R.","Pohl T.M.","Eger P.","Zimmermann W.","Wedler H.","Wambutt R.","Purnelle B.","Goffeau A.","Cadieu E.","Dreano S.","Gloux S.","Lelaure V.","Mottier S.","Galibert F.","Aves S.J.","Xiang Z.","Hunt C.","Moore K.","Hurst S.M.","Lucas M.","Rochet M.","Gaillardin C.","Tallada V.A.","Garzon A.","Thode G.","Daga R.R.","Cruzado L.","Jimenez J.","Sanchez M.","del Rey F.","Benito J.","Dominguez A.","Revuelta J.L.","Moreno S.","Armstrong J.","Forsburg S.L.","Cerutti L.","Lowe T.","McCombie W.R.","Paulsen I.","Potashkin J.","Shpakovski G.V.","Ussery D.","Barrell B.G.","Nurse P."],"citationCrossReferences":[{"database":"PubMed","id":"11859360"},{"database":"DOI","id":"10.1038/nature724"}],"title":"The genome sequence of Schizosaccharomyces pombe.","publicationDate":"2002","journal":"Nature","firstPage":"871","lastPage":"880","volume":"415"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"972 / ATCC 24843","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"12845604","citationType":"journal article","authors":["De Groot P.W.","Hellingwerf K.J.","Klis F.M."],"citationCrossReferences":[{"database":"PubMed","id":"12845604"},{"database":"DOI","id":"10.1002/yea.1007"}],"title":"Genome-wide identification of fungal GPI proteins.","publicationDate":"2003","journal":"Yeast","firstPage":"781","lastPage":"796","volume":"20"},"referencePositions":["PREDICTION OF GPI-ANCHOR"]},{"referenceNumber":3,"citation":{"id":"16751704","citationType":"journal article","authors":["Morita T.","Tanaka N.","Hosomi A.","Giga-Hama Y.","Takegawa K."],"citationCrossReferences":[{"database":"PubMed","id":"16751704"},{"database":"DOI","id":"10.1271/bbb.50693"}],"title":"An alpha-amylase homologue, aah3, encodes a GPI-anchored membrane protein required for cell wall integrity and morphogenesis in Schizosaccharomyces pombe.","publicationDate":"2006","journal":"Biosci. Biotechnol. Biochem.","firstPage":"1454","lastPage":"1463","volume":"70"},"referencePositions":["FUNCTION","GPI-ANCHOR","GLYCOSYLATION","MUTAGENESIS OF ASP-229 AND GLU-253"]},{"referenceNumber":4,"citation":{"id":"16823372","citationType":"journal article","authors":["Matsuyama A.","Arai R.","Yashiroda Y.","Shirai A.","Kamata A.","Sekido S.","Kobayashi Y.","Hashimoto A.","Hamamoto M.","Hiraoka Y.","Horinouchi S.","Yoshida M."],"citationCrossReferences":[{"database":"PubMed","id":"16823372"},{"database":"DOI","id":"10.1038/nbt1222"}],"title":"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.","publicationDate":"2006","journal":"Nat. Biotechnol.","firstPage":"841","lastPage":"847","volume":"24"},"referencePositions":["SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]"]},{"referenceNumber":5,"citation":{"id":"40668835","citationType":"journal article","authors":["Jacob A.","Willet A.H.","Igarashi M.G.","El Hariri El Nokab M.","Turner L.A.","Alsanad A.K.A.","Wang T.","Gould K.L."],"citationCrossReferences":[{"database":"PubMed","id":"40668835"},{"database":"DOI","id":"10.1073/pnas.2505509122"}],"title":"alpha-glucan remodeling by GH13-domain enzymes shapes fungal cell wall architecture.","publicationDate":"2025","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"e2505509122","lastPage":"e2505509122","volume":"122"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION","DISRUPTION PHENOTYPE"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CU329672","properties":[{"key":"ProteinId","value":"CAB40006.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"T41503","properties":[{"key":"EntryName","value":"T41503"}]},{"database":"RefSeq","id":"NP_587976.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001022967.3"}]},{"database":"AlphaFoldDB","id":"Q9Y7S9","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9Y7S9","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"275980","properties":[{"key":"Interactions","value":"4"}]},{"database":"FunCoup","id":"Q9Y7S9","properties":[{"key":"Number of interactors","value":"132"}]},{"database":"STRING","id":"284812.Q9Y7S9","properties":[{"key":"Description","value":"-"}]},{"database":"GlyCosmos","id":"Q9Y7S9","properties":[{"key":"glycosylation","value":"7 sites, No reported glycans"}]},{"database":"iPTMnet","id":"Q9Y7S9","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"284812-Q9Y7S9","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"2539415","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"spo:2539415","properties":[{"key":"Description","value":"-"}]},{"database":"PomBase","id":"SPCC63.02c","properties":[{"key":"GeneName","value":"aah3"}]},{"database":"VEuPathDB","id":"FungiDB:SPCC63.02c","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG0471","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_006462_7_2_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q9Y7S9","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"DAVKMMN","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"PhylomeDB","id":"Q9Y7S9","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:Q9Y7S9","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000002485","properties":[{"key":"Component","value":"Chromosome III"}]},{"database":"GO","id":"GO:0009897","properties":[{"key":"GoTerm","value":"C:external side of plasma membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"GO","id":"GO:0009277","properties":[{"key":"GoTerm","value":"C:fungal-type cell wall"},{"key":"GoEvidenceType","value":"IDA:PomBase"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"12845604"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IDA:PomBase"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"12845604"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16751704"}]},{"database":"GO","id":"GO:0004134","properties":[{"key":"GoTerm","value":"F:4-alpha-glucanotransferase activity"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0004556","properties":[{"key":"GoTerm","value":"F:alpha-amylase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0005509","properties":[{"key":"GoTerm","value":"F:calcium ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0070591","properties":[{"key":"GoTerm","value":"P:ascospore wall biogenesis"},{"key":"GoEvidenceType","value":"IMP:PomBase"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"16751704"}]},{"database":"GO","id":"GO:0016052","properties":[{"key":"GoTerm","value":"P:carbohydrate catabolic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0070600","properties":[{"key":"GoTerm","value":"P:fungal-type cell wall (1->3)-alpha-glucan biosynthetic process"},{"key":"GoEvidenceType","value":"IMP:PomBase"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"40668835"}]},{"database":"GO","id":"GO:0071940","properties":[{"key":"GoTerm","value":"P:fungal-type cell wall assembly"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0051278","properties":[{"key":"GoTerm","value":"P:fungal-type cell wall polysaccharide biosynthetic process"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"CDD","id":"cd11319","properties":[{"key":"EntryName","value":"AmyAc_euk_AmyA"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.60.40.1180:FF:000037","properties":[{"key":"EntryName","value":"Alpha-amylase A"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.20.20.80:FF:000120","properties":[{"key":"EntryName","value":"Alpha-amylase A"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.20.20.80","properties":[{"key":"EntryName","value":"Glycosidases"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.60.40.1180","properties":[{"key":"EntryName","value":"Golgi alpha-mannosidase II"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR013777","properties":[{"key":"EntryName","value":"A-amylase-like"}]},{"database":"InterPro","id":"IPR015340","properties":[{"key":"EntryName","value":"A_amylase_C_dom"}]},{"database":"InterPro","id":"IPR006047","properties":[{"key":"EntryName","value":"GH13_cat_dom"}]},{"database":"InterPro","id":"IPR017853","properties":[{"key":"EntryName","value":"GH_hydrolase_sf"}]},{"database":"InterPro","id":"IPR013780","properties":[{"key":"EntryName","value":"Glyco_hydro_b"}]},{"database":"PANTHER","id":"PTHR10357:SF224","properties":[{"key":"EntryName","value":"ALPHA-AMYLASE 3"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10357","properties":[{"key":"EntryName","value":"ALPHA-AMYLASE FAMILY MEMBER"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF09260","properties":[{"key":"EntryName","value":"A_amylase_dom_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00128","properties":[{"key":"EntryName","value":"Alpha-amylase"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF001024","properties":[{"key":"EntryName","value":"Alph-amyl_fung"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00642","properties":[{"key":"EntryName","value":"Aamy"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51445","properties":[{"key":"EntryName","value":"(Trans)glycosidases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51011","properties":[{"key":"EntryName","value":"Glycosyl hydrolase domain"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MFGVYFVLLFLSSALIHVANAGSNAEWRKRIIYQILTDRFAVDDGSTDNPCDPDANQYCGGTWKGIENKLDYIEDMGFNAIWISPIDKNIEGDIDGAGYAYHGYWNTDYESLNEHFGTEDDLVSLITAAHKAGIWVMLDSIVNSMALAPPLADADYSSLNPFNKESYFHPYCLIDWDITDNETNVMDCWQDSGVLLADLDVESSDVSSYLSDHFKSLISKYDFDGLRIDAVKMMNYTFFPDFVDATGVYSVGEVFSYDPDTMCSYMSVLPGVTNYFLQLYINFSFTATGAGFTLIPTYQEVMASNCSKYDSTLMLTFIENHDLYRFPYYTSDQSQIMGALSFVLIWDGIPSIFYGQEQGFNGGEDPANRPALWLTDYDQSNPYYTVIKTMVAFRKFVITQDPDWVTSTYQSIESAVDHYVGQKNDVLVMFNNMGVTNNLTIYEVETNYTANEVVSDVFGHRTLTVGADKTLTASMTNGYPLIMYPHSKMSGFTLPTVNRTVMPSTSATATTTVYTSYYSPSYSARSFTGTGSIFTISSSSRLILSFKTLVFGLGVTAMLFVLFF","length":564,"molWeight":63206,"crc64":"1229FD4AEC702FD0","md5":"A62C8E2E908DBE418367857173B6F9F3"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"SUBCELLULAR LOCATION":1,"PTM":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Signal":1,"Chain":1,"Propeptide":1,"Active site":2,"Binding site":10,"Site":1,"Lipidation":1,"Glycosylation":7,"Disulfide bond":3,"Mutagenesis":2},"uniParcId":"UPI0000125A5B"}}