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Accepts the ubiquitin-like modifier UFM1 from the E1 enzyme UBA5 and forms an intermediate with UFM1 via a thioester linkage (PubMed:15071506, PubMed:29868776, PubMed:34588452, PubMed:38383789). Ufmylation is involved in various processes, such as ribosome recycling, response to DNA damage, interferon response or reticulophagy (also called ER-phagy) (PubMed:27351204, PubMed:32160526, PubMed:35394863, PubMed:37036982, PubMed:38383789)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17825256"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20018847"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25902260"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27653677"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29868776"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30886146"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34588452"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37988244"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38383789"}],"value":"Interacts with UBA5 (via C-terminus) (PubMed:17825256, PubMed:27653677, PubMed:29868776, PubMed:34588452). Interacts with UFL1 (PubMed:20018847, PubMed:30886146, PubMed:37988244, PubMed:38383789). Interacts with UFM1 (PubMed:29868776). Interacts with KIRREL3 (PubMed:25902260)"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"Q9Y3C8","intActId":"EBI-1045733"},"interactantTwo":{"uniProtKBAccession":"X5D778","geneName":"ANKRD11","intActId":"EBI-17183751"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q9Y3C8","intActId":"EBI-1045733"},"interactantTwo":{"uniProtKBAccession":"Q96JB5","geneName":"CDK5RAP3","intActId":"EBI-718818"},"numberOfExperiments":7,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q9Y3C8","intActId":"EBI-1045733"},"interactantTwo":{"uniProtKBAccession":"P57678","geneName":"GEMIN4","intActId":"EBI-356700"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q9Y3C8","intActId":"EBI-1045733"},"interactantTwo":{"uniProtKBAccession":"Q8IZU9","geneName":"KIRREL3","intActId":"EBI-16427312"},"numberOfExperiments":4,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q9Y3C8","intActId":"EBI-1045733"},"interactantTwo":{"uniProtKBAccession":"Q96HA8","geneName":"NTAQ1","intActId":"EBI-741158"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q9Y3C8","intActId":"EBI-1045733"},"interactantTwo":{"uniProtKBAccession":"O60260-5","geneName":"PRKN","intActId":"EBI-21251460"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q9Y3C8","intActId":"EBI-1045733"},"interactantTwo":{"uniProtKBAccession":"O94874","geneName":"UFL1","intActId":"EBI-1048088"},"numberOfExperiments":9,"organismDiffer":false}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34588452"}],"value":"In absence of UBA5, the active site is solvated by water molecules thereby reducing its nucleophilic activity (PubMed:34588452). 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