{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9Y231","secondaryAccessions":["Q5T0W4"],"uniProtkbId":"FUT9_HUMAN","entryAudit":{"firstPublicDate":"2004-11-09","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2010-11-02","entryVersion":178,"sequenceVersion":2},"annotationScore":5.0,"organism":{"scientificName":"Homo sapiens","commonName":"Human","taxonId":9606,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Primates","Haplorrhini","Catarrhini","Hominidae","Homo"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase 9"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10622713"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11278338"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12107078"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18395013"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23192350"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23263199"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37202521"}],"value":"2.4.1.152"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"23000574"}],"value":"Fucosyltransferase 9"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"10386598"}],"value":"Fucosyltransferase IX"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"10386598"}],"value":"Fuc-TIX"},{"value":"FucT-IX"}]},{"fullName":{"value":"Galactoside 3-L-fucosyltransferase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"10929005"},{"evidenceCode":"ECO:0000312","source":"HGNC","id":"HGNC:4020"}],"value":"FUT9"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O88819"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10386598"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10622713"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11278338"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12107078"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16282604"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17335083"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18395013"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23000574"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23192350"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23263199"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29593094"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37202521"}],"value":"Catalyzes alpha(1->3) linkage of fucosyl moiety transferred from GDP-beta-L-fucose to N-acetyl glucosamine (GlcNAc) within type 2 lactosamine (LacNAc, beta-D-Gal-(1->4)-beta-D-GlcNAc-) glycan attached to glycolipids and N- or O-linked glycoproteins. Fucosylates distal type 2 LacNAc and its fucosylated (H-type 2 LacNAc) and sialylated (sialyl-type 2 LacNAc) derivatives to form Lewis x (Lex) (CD15) and Lewis y (Ley) antigenic epitopes involved in cell adhesion and differentiation (PubMed:10386598, PubMed:10622713, PubMed:11278338, PubMed:12107078, PubMed:16282604, PubMed:17335083, PubMed:18395013, PubMed:23192350, PubMed:23263199, PubMed:29593094, PubMed:37202521). Generates Lex epitopes in the brain, presumably playing a role in the maintenance of neuronal stemness and neurite outgrowth in progenitor neural cells (By similarity) (PubMed:17335083, PubMed:23000574). Fucosylates the internal type 2 LacNAc unit of the polylactosamine chain to form VIM-2 antigen that serves as recognition epitope for SELE (PubMed:23192350). Can also modify milk oligosaccharides, in particular type 2 tetrasaccharide LNnT (PubMed:37202521)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl derivative + GDP-beta-L-fucose = a beta-D-galactosyl-(1->4)-[alpha-L-fucosyl-(1->3)]-N-acetyl-beta-D-glucosaminyl derivative + GDP + 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ACTIVITY","reaction":{"name":"an alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc derivative + GDP-beta-L-fucose = an alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-[alpha-L-Fuc-(1->3)]-beta-D-GlcNAc derivative + GDP + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:68044"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57273"},{"database":"ChEBI","id":"CHEBI:58189"},{"database":"ChEBI","id":"CHEBI:145343"},{"database":"ChEBI","id":"CHEBI:176900"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11278338"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:68045"},"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"11278338"}]}]},{"commentType":"CATALYTIC 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H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:48388"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57273"},{"database":"ChEBI","id":"CHEBI:58189"},{"database":"ChEBI","id":"CHEBI:90383"},{"database":"ChEBI","id":"CHEBI:90384"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O88819"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:48389"},"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O88819"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"alpha-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1')-ceramide + GDP-beta-L-fucose = a neolactoside IV(3)-alpha-Gal,III(3)-alpha-Fuc-nLc4Cer + GDP + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:48380"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57273"},{"database":"ChEBI","id":"CHEBI:58189"},{"database":"ChEBI","id":"CHEBI:90380"},{"database":"ChEBI","id":"CHEBI:90381"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O88819"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:48381"},"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O88819"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a neolactoside nLc4Cer + GDP-beta-L-fucose = a neolactoside III(3)-alpha-Fuc-nLc4Cer + GDP + 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H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:56076"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57273"},{"database":"ChEBI","id":"CHEBI:58189"},{"database":"ChEBI","id":"CHEBI:136545"},{"database":"ChEBI","id":"CHEBI:139509"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37202521"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:56077"},"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37202521"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-D-Glc + GDP-beta-L-fucose = beta-D-Gal-(1->4)-[alpha-L-Fuc-(1->3)]-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-D-Glc + GDP + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:77187"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57273"},{"database":"ChEBI","id":"CHEBI:58189"},{"database":"ChEBI","id":"CHEBI:60239"},{"database":"ChEBI","id":"CHEBI:61352"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37202521"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:77188"},"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37202521"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"an alpha-L-Fuc-(1->2)-beta-D-Gal-(1->4)-beta-D-GlcNAc derivative + GDP-beta-L-fucose = an alpha-L-Fuc-(1->2)-beta-D-Gal-(1->4)-[alpha-L-Fuc-(1->3)]-beta-D-GlcNAc derivative + GDP + 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Highly expressed in granulocytes. Not expressed in monocytes (PubMed:11278338)"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37202521"}],"value":"The donor-binding domain adopts a Rossman-like fold involved in GDP-beta-L-fucose sugar donor interactions"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37202521"}],"value":"The acceptor-binding domain adopts a Rossman-like fold consisting of six-stranded parallel beta sheets characteristic of the Toll/interleukin-1 receptor (TIR) fold family. Interacts with the LacNAc unit of type 2 LacNAc and H-type 2 LacNAc structures. It contains the catalytic base Glu-137 which deprotonates the hydroxyl group of GlcNAc while forming bridging interactions with the donor sugar to position the catalytic machinery in the active site"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18395013"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37202521"}],"value":"N-glycosylated with complex-type N-glycans. The glycan alpha-D-Man-(1->3)-beta-D-Man-(1->4)-GlcNAc-(1->4)-GlcNAc is attached at Asn-153"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the glycosyltransferase 10 family"}],"commentType":"SIMILARITY"},{"commentType":"WEB RESOURCE","resourceName":"Functional Glycomics Gateway - GTase","resourceUrl":"http://www.functionalglycomics.org/glycomics/molecule/jsp/glycoEnzyme/viewGlycoEnzyme.jsp?gbpId=gt_hum_606","ftp":false,"note":"Fucosyltransferase 9"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":359,"modifier":"EXACT"}},"description":"4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase 9","featureId":"PRO_0000221118"},{"type":"Topological 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