{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9XWF4","secondaryAccessions":["Q7K779"],"uniProtkbId":"IMPCT_CAEEL","entryAudit":{"firstPublicDate":"2008-04-29","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1999-11-01","entryVersion":134,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Caenorhabditis elegans","taxonId":6239,"lineage":["Eukaryota","Metazoa","Ecdysozoa","Nematoda","Chromadorea","Rhabditida","Rhabditina","Rhabditomorpha","Rhabditoidea","Rhabditidae","Peloderinae","Caenorhabditis"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"value":"Protein IMPACT homolog"}}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000312","source":"WormBase","id":"Y52B11A.2a"}],"value":"impt-1"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"WormBase","id":"Y52B11A.2a"}],"value":"Y52B11A.2"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O55091"}],"value":"Translational regulator that ensures constant high levels of translation under amino acid starvation. Plays a role as a negative regulator of the gcn-2 kinase activity; impairs gcn-1-mediated gcn-2 activation, and hence gcn-2-mediated eIF-2-alpha phosphorylation and subsequent down-regulation of protein synthesis in amino acid-starved cells. Plays a role in differentiation of neuronal cells by stimulating neurite outgrowth"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O55091"}],"value":"Interacts with gcn-1; prevents the interaction of gcn-1 with gcn-2 and inhibits gcn-2 kinase activity. Interaction with rpl-39; this interaction occurs in a gcn-1-independent manner. Associates with ribosomes; this interaction occurs in a gcn-1-independent manner. Associates with actin; this interaction occurs in a gcn-1-independent manner"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O55091"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"commentType":"ALTERNATIVE PRODUCTS","events":["Alternative splicing"],"isoforms":[{"name":{"evidences":[{"evidenceCode":"ECO:0000312","source":"WormBase","id":"Y52B11A.2a"}],"value":"a"},"isoformIds":["Q9XWF4-1"],"isoformSequenceStatus":"Displayed"},{"name":{"evidences":[{"evidenceCode":"ECO:0000312","source":"WormBase","id":"Y52B11A.2a"}],"value":"b"},"isoformIds":["Q9XWF4-2"],"sequenceIds":["VSP_033137","VSP_033138"],"isoformSequenceStatus":"Described"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the IMPACT family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":257,"modifier":"EXACT"}},"description":"Protein IMPACT homolog","featureId":"PRO_0000330858"},{"type":"Domain","location":{"start":{"value":9,"modifier":"EXACT"},"end":{"value":102,"modifier":"EXACT"}},"description":"RWD","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00179"}]},{"type":"Alternative sequence","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":101,"modifier":"EXACT"}},"description":"in isoform b","evidences":[{"evidenceCode":"ECO:0000305"}],"featureId":"VSP_033137","alternativeSequence":{"originalSequence":"D","alternativeSequences":["F"]}},{"type":"Alternative sequence","location":{"start":{"value":102,"modifier":"EXACT"},"end":{"value":257,"modifier":"EXACT"}},"description":"in isoform b","evidences":[{"evidenceCode":"ECO:0000305"}],"featureId":"VSP_033138","alternativeSequence":{}}],"keywords":[{"id":"KW-0025","category":"Coding sequence diversity","name":"Alternative splicing"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0221","category":"Biological process","name":"Differentiation"},{"id":"KW-0524","category":"Biological process","name":"Neurogenesis"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0678","category":"Molecular function","name":"Repressor"},{"id":"KW-0346","category":"Biological process","name":"Stress response"},{"id":"KW-0810","category":"Biological process","name":"Translation regulation"}],"references":[{"referenceNumber":1,"citation":{"id":"9851916","citationType":"journal article","authoringGroup":["The C. elegans sequencing consortium"],"citationCrossReferences":[{"database":"PubMed","id":"9851916"},{"database":"DOI","id":"10.1126/science.282.5396.2012"}],"title":"Genome sequence of the nematode C. elegans: a platform for investigating biology.","publicationDate":"1998","journal":"Science","firstPage":"2012","lastPage":"2018","volume":"282"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]","ALTERNATIVE SPLICING"],"referenceComments":[{"value":"Bristol N2","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AL032654","properties":[{"key":"ProteinId","value":"CAA21718.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AL032654","properties":[{"key":"ProteinId","value":"CAE54915.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"T27104","properties":[{"key":"EntryName","value":"T27104"}]},{"database":"RefSeq","id":"NP_001021782.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001026611.4"}],"isoformId":"Q9XWF4-1"},{"database":"RefSeq","id":"NP_001379419.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001392943.1"}],"isoformId":"Q9XWF4-2"},{"database":"AlphaFoldDB","id":"Q9XWF4","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9XWF4","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"38410","properties":[{"key":"Interactions","value":"7"}]},{"database":"FunCoup","id":"Q9XWF4","properties":[{"key":"Number of interactors","value":"2673"}]},{"database":"STRING","id":"6239.Y52B11A.2a.1","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"6239-Y52B11A.2a.1","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"Q9XWF4","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblMetazoa","id":"Y52B11A.2a.1","properties":[{"key":"ProteinId","value":"Y52B11A.2a.1"},{"key":"GeneId","value":"WBGene00013122"}]},{"database":"EnsemblMetazoa","id":"Y52B11A.2b.1","properties":[{"key":"ProteinId","value":"Y52B11A.2b.1"},{"key":"GeneId","value":"WBGene00013122"}]},{"database":"GeneID","id":"173001","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"cel:CELE_Y52B11A.2","properties":[{"key":"Description","value":"-"}]},{"database":"UCSC","id":"Y52B11A.2a","properties":[{"key":"OrganismName","value":"c. elegans"}],"isoformId":"Q9XWF4-1"},{"database":"AGR","id":"WB:WBGene00013122","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"173001","properties":[{"key":"Description","value":"-"}]},{"database":"WormBase","id":"Y52B11A.2a","properties":[{"key":"ProteinId","value":"CE20291"},{"key":"GeneId","value":"WBGene00013122"},{"key":"GeneName","value":"impt-1"}],"isoformId":"Q9XWF4-1"},{"database":"WormBase","id":"Y52B11A.2b","properties":[{"key":"ProteinId","value":"CE36243"},{"key":"GeneId","value":"WBGene00013122"},{"key":"GeneName","value":"impt-1"}],"isoformId":"Q9XWF4-2"},{"database":"eggNOG","id":"KOG3299","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_045276_1_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q9XWF4","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"FRHICNL","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"69641at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q9XWF4","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:Q9XWF4","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000001940","properties":[{"key":"Component","value":"Chromosome I"}]},{"database":"Bgee","id":"WBGene00013122","properties":[{"key":"ExpressionPatterns","value":"Expressed in germ line (C elegans) and 4 other cell types or tissues"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0140311","properties":[{"key":"GoTerm","value":"F:protein sequestering activity"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0034198","properties":[{"key":"GoTerm","value":"P:cellular response to amino acid starvation"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0140469","properties":[{"key":"GoTerm","value":"P:GCN2-mediated signaling"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:1990611","properties":[{"key":"GoTerm","value":"P:regulation of cytoplasmic translational initiation in response to stress"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0006446","properties":[{"key":"GoTerm","value":"P:regulation of translational initiation"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"CDD","id":"cd23821","properties":[{"key":"EntryName","value":"RWD_IMPACT"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.230.30","properties":[{"key":"EntryName","value":"Impact, N-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.10.110.10","properties":[{"key":"EntryName","value":"Ubiquitin Conjugating Enzyme"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR023582","properties":[{"key":"EntryName","value":"Impact"}]},{"database":"InterPro","id":"IPR001498","properties":[{"key":"EntryName","value":"Impact_N"}]},{"database":"InterPro","id":"IPR036956","properties":[{"key":"EntryName","value":"Impact_N_sf"}]},{"database":"InterPro","id":"IPR020568","properties":[{"key":"EntryName","value":"Ribosomal_Su5_D2-typ_SF"}]},{"database":"InterPro","id":"IPR006575","properties":[{"key":"EntryName","value":"RWD_dom"}]},{"database":"InterPro","id":"IPR016135","properties":[{"key":"EntryName","value":"UBQ-conjugating_enzyme/RWD"}]},{"database":"PANTHER","id":"PTHR16301","properties":[{"key":"EntryName","value":"IMPACT-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR16301:SF25","properties":[{"key":"EntryName","value":"PROTEIN IMPACT"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01205","properties":[{"key":"EntryName","value":"Impact_N"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF05773","properties":[{"key":"EntryName","value":"RWD"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00591","properties":[{"key":"EntryName","value":"RWD"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF54211","properties":[{"key":"EntryName","value":"Ribosomal protein S5 domain 2-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF54495","properties":[{"key":"EntryName","value":"UBC-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50908","properties":[{"key":"EntryName","value":"RWD"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSDEEQRIAEIESLASIFPDLLEETTDKQLVFKFDRDIGMTINLPEDYPSISPPIFELSGPYLRREQKEVLHNSLNDVYIENIGFPVIFNWISLVQDFIRDLPEEVAEPSHVADDVVTPMAEEIDDMNIRHGEVLTDRKSAFQAHLAEVRSKEDVDRVMRILKSNTKICRATHNITAYRYMTEINGKPIHHHDCVDDGEFGASSKMLELMDRMKADNVMVVVSRWYGGIHLGPDRFRHINNLTRQILSDNNYGPKKS","length":257,"molWeight":29599,"crc64":"739FCA5A563C2628","md5":"D889018CB164A0BB0200E0FC60F5D1F1"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"ALTERNATIVE PRODUCTS":2,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Domain":1,"Alternative sequence":2},"uniParcId":"UPI000007FFE0"}}