{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9XB61","uniProtkbId":"CARA_PECCC","entryAudit":{"firstPublicDate":"2013-10-16","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1999-11-01","entryVersion":79,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Pectobacterium carotovorum subsp. carotovorum","commonName":"Erwinia carotovora subsp. carotovora","taxonId":555,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Pectobacteriaceae","Pectobacterium"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Carbapenam-3-carboxylate synthase"},"ecNumbers":[{"value":"6.3.3.6"}]},"alternativeNames":[{"fullName":{"value":"Carbapenam-3-carboxylate ligase"}}]},"genes":[{"geneName":{"value":"carA"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12820893"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17658887"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19371088"}],"value":"Involved in the biosynthesis of carbapenam-3-carboxylate, a beta-lactam antibiotic of the carbapenem class. Catalyzes the ATP-dependent formation of (3S,5S)-carbapenam-3-carboxylate from (2S,5S)-5-carboxymethylproline"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2S,5S)-5-carboxymethylproline + ATP = (3S,5S)-carbapenam-3-caboxylate + AMP + diphosphate + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:36703"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:73939"},{"database":"ChEBI","id":"CHEBI:73962"},{"database":"ChEBI","id":"CHEBI:456215"}],"ecNumber":"6.3.3.6","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12820893"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17658887"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19371088"}]}},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":0.11,"unit":"mM","substrate":"ATP","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12820893"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17658887"}]},{"constant":0.23,"unit":"mM","substrate":"(2S,5S)-5-carboxymethylproline","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12820893"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17658887"}]}],"note":{"texts":[{"value":"kcat is 0.28 sec(-1)."}]}},"phDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12820893"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17658887"}],"value":"Optimum pH is 8.5."}]}},{"texts":[{"value":"Antibiotic biosynthesis; carbapenem biosynthesis"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12890666"}],"value":"Homotetramer"}],"commentType":"SUBUNIT"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":503,"modifier":"EXACT"}},"description":"Carbapenam-3-carboxylate synthase","featureId":"PRO_0000424221"},{"type":"Domain","location":{"start":{"value":228,"modifier":"EXACT"},"end":{"value":443,"modifier":"EXACT"}},"description":"Asparagine synthetase"},{"type":"Active site","location":{"start":{"value":345,"modifier":"EXACT"},"end":{"value":345,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"19371088"}]},{"type":"Active site","location":{"start":{"value":380,"modifier":"EXACT"},"end":{"value":380,"modifier":"EXACT"}},"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"19371088"}]},{"type":"Binding site","location":{"start":{"value":244,"modifier":"EXACT"},"end":{"value":251,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12890666"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":270,"modifier":"EXACT"},"end":{"value":270,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12890666"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":344,"modifier":"EXACT"},"end":{"value":348,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12890666"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":346,"modifier":"EXACT"},"end":{"value":346,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12890666"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":371,"modifier":"EXACT"},"end":{"value":371,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12890666"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":374,"modifier":"EXACT"},"end":{"value":374,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12890666"}],"ligand":{"name":"substrate"}},{"type":"Binding site","location":{"start":{"value":421,"modifier":"EXACT"},"end":{"value":421,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12890666"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":444,"modifier":"EXACT"},"end":{"value":446,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12890666"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Mutagenesis","location":{"start":{"value":345,"modifier":"EXACT"},"end":{"value":345,"modifier":"EXACT"}},"description":"Loss of activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19371088"}],"alternativeSequence":{"originalSequence":"Y","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":345,"modifier":"EXACT"},"end":{"value":345,"modifier":"EXACT"}},"description":"Reduces catalytic efficiency.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19371088"}],"alternativeSequence":{"originalSequence":"Y","alternativeSequences":["F"]}},{"type":"Mutagenesis","location":{"start":{"value":380,"modifier":"EXACT"},"end":{"value":380,"modifier":"EXACT"}},"description":"Loss of activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19371088"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":380,"modifier":"EXACT"},"end":{"value":380,"modifier":"EXACT"}},"description":"Reduces catalytic efficiency.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19371088"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["D"]}},{"type":"Mutagenesis","location":{"start":{"value":380,"modifier":"EXACT"},"end":{"value":380,"modifier":"EXACT"}},"description":"Reduces catalytic efficiency.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19371088"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["Q"]}},{"type":"Mutagenesis","location":{"start":{"value":443,"modifier":"EXACT"},"end":{"value":443,"modifier":"EXACT"}},"description":"Loss of activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17658887"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["A","M"]}},{"type":"Beta strand","location":{"start":{"value":5,"modifier":"EXACT"},"end":{"value":9,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Helix","location":{"start":{"value":13,"modifier":"EXACT"},"end":{"value":20,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Beta strand","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":30,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Beta strand","location":{"start":{"value":33,"modifier":"EXACT"},"end":{"value":38,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Beta strand","location":{"start":{"value":43,"modifier":"EXACT"},"end":{"value":47,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Beta strand","location":{"start":{"value":49,"modifier":"EXACT"},"end":{"value":57,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Helix","location":{"start":{"value":62,"modifier":"EXACT"},"end":{"value":69,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Turn","location":{"start":{"value":70,"modifier":"EXACT"},"end":{"value":72,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Helix","location":{"start":{"value":74,"modifier":"EXACT"},"end":{"value":78,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Helix","location":{"start":{"value":81,"modifier":"EXACT"},"end":{"value":92,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Helix","location":{"start":{"value":94,"modifier":"EXACT"},"end":{"value":99,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Beta strand","location":{"start":{"value":102,"modifier":"EXACT"},"end":{"value":109,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Beta strand","location":{"start":{"value":115,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Beta strand","location":{"start":{"value":121,"modifier":"EXACT"},"end":{"value":125,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Beta strand","location":{"start":{"value":128,"modifier":"EXACT"},"end":{"value":139,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Helix","location":{"start":{"value":141,"modifier":"EXACT"},"end":{"value":148,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Helix","location":{"start":{"value":158,"modifier":"EXACT"},"end":{"value":161,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Beta strand","location":{"start":{"value":177,"modifier":"EXACT"},"end":{"value":179,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Beta strand","location":{"start":{"value":182,"modifier":"EXACT"},"end":{"value":190,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Beta strand","location":{"start":{"value":196,"modifier":"EXACT"},"end":{"value":203,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Helix","location":{"start":{"value":206,"modifier":"EXACT"},"end":{"value":208,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Helix","location":{"start":{"value":216,"modifier":"EXACT"},"end":{"value":234,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Helix","location":{"start":{"value":235,"modifier":"EXACT"},"end":{"value":237,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Beta strand","location":{"start":{"value":239,"modifier":"EXACT"},"end":{"value":244,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Helix","location":{"start":{"value":249,"modifier":"EXACT"},"end":{"value":258,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Turn","location":{"start":{"value":259,"modifier":"EXACT"},"end":{"value":261,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Beta strand","location":{"start":{"value":263,"modifier":"EXACT"},"end":{"value":272,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Helix","location":{"start":{"value":278,"modifier":"EXACT"},"end":{"value":288,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Beta strand","location":{"start":{"value":291,"modifier":"EXACT"},"end":{"value":297,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Helix","location":{"start":{"value":299,"modifier":"EXACT"},"end":{"value":313,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Helix","location":{"start":{"value":318,"modifier":"EXACT"},"end":{"value":334,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Beta strand","location":{"start":{"value":338,"modifier":"EXACT"},"end":{"value":342,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Helix","location":{"start":{"value":347,"modifier":"EXACT"},"end":{"value":351,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Turn","location":{"start":{"value":352,"modifier":"EXACT"},"end":{"value":354,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Helix","location":{"start":{"value":364,"modifier":"EXACT"},"end":{"value":378,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Helix","location":{"start":{"value":384,"modifier":"EXACT"},"end":{"value":388,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Beta strand","location":{"start":{"value":392,"modifier":"EXACT"},"end":{"value":394,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Helix","location":{"start":{"value":400,"modifier":"EXACT"},"end":{"value":407,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Helix","location":{"start":{"value":411,"modifier":"EXACT"},"end":{"value":413,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Beta strand","location":{"start":{"value":419,"modifier":"EXACT"},"end":{"value":421,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Helix","location":{"start":{"value":422,"modifier":"EXACT"},"end":{"value":430,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Helix","location":{"start":{"value":436,"modifier":"EXACT"},"end":{"value":439,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Helix","location":{"start":{"value":441,"modifier":"EXACT"},"end":{"value":443,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q19"}]},{"type":"Helix","location":{"start":{"value":446,"modifier":"EXACT"},"end":{"value":449,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q19"}]},{"type":"Helix","location":{"start":{"value":452,"modifier":"EXACT"},"end":{"value":460,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Helix","location":{"start":{"value":467,"modifier":"EXACT"},"end":{"value":482,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]},{"type":"Helix","location":{"start":{"value":488,"modifier":"EXACT"},"end":{"value":490,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q19"}]},{"type":"Helix","location":{"start":{"value":493,"modifier":"EXACT"},"end":{"value":500,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Q15"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0045","category":"Biological process","name":"Antibiotic biosynthesis"},{"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"id":"KW-0436","category":"Molecular function","name":"Ligase"},{"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"}],"references":[{"referenceNumber":1,"citation":{"id":"9402024","citationType":"journal article","authors":["McGowan S.J.","Sebaihia M.","O'Leary S.","Hardie K.R.","Williams P.","Stewart G.S.","Bycroft B.W.","Salmond G.P."],"citationCrossReferences":[{"database":"PubMed","id":"9402024"},{"database":"DOI","id":"10.1046/j.1365-2958.1997.6001974.x"}],"title":"Analysis of the carbapenem gene cluster of Erwinia carotovora: definition of the antibiotic biosynthetic genes and evidence for a novel beta-lactam resistance mechanism.","publicationDate":"1997","journal":"Mol. Microbiol.","firstPage":"545","lastPage":"556","volume":"26"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 39048 / GS101 / SC 12","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"12820893","citationType":"journal article","authors":["Gerratana B.","Stapon A.","Townsend C.A."],"citationCrossReferences":[{"database":"PubMed","id":"12820893"},{"database":"DOI","id":"10.1021/bi034361d"}],"title":"Inhibition and alternate substrate studies on the mechanism of carbapenam synthetase from Erwinia carotovora.","publicationDate":"2003","journal":"Biochemistry","firstPage":"7836","lastPage":"7847","volume":"42"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY","BIOPHYSICOCHEMICAL PROPERTIES"]},{"referenceNumber":3,"citation":{"id":"17658887","citationType":"journal article","authors":["Arnett S.O.","Gerratana B.","Townsend C.A."],"citationCrossReferences":[{"database":"PubMed","id":"17658887"},{"database":"DOI","id":"10.1021/bi0618464"}],"title":"Rate-limiting steps and role of active site Lys443 in the mechanism of carbapenam synthetase.","publicationDate":"2007","journal":"Biochemistry","firstPage":"9337","lastPage":"9345","volume":"46"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY","BIOPHYSICOCHEMICAL PROPERTIES","MUTAGENESIS OF LYS-443"]},{"referenceNumber":4,"citation":{"id":"19371088","citationType":"journal article","authors":["Raber M.L.","Arnett S.O.","Townsend C.A."],"citationCrossReferences":[{"database":"PubMed","id":"19371088"},{"database":"DOI","id":"10.1021/bi900432n"}],"title":"A conserved tyrosyl-glutamyl catalytic dyad in evolutionarily linked enzymes: carbapenam synthetase and beta-lactam synthetase.","publicationDate":"2009","journal":"Biochemistry","firstPage":"4959","lastPage":"4971","volume":"48"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY","ACTIVE SITE","MUTAGENESIS OF TYR-345 AND GLU-380"]},{"referenceNumber":5,"citation":{"id":"12890666","citationType":"journal article","authors":["Miller M.T.","Gerratana B.","Stapon A.","Townsend C.A.","Rosenzweig A.C."],"citationCrossReferences":[{"database":"PubMed","id":"12890666"},{"database":"DOI","id":"10.1074/jbc.m307901200"}],"title":"Crystal structure of carbapenam synthetase (CarA).","publicationDate":"2003","journal":"J. Biol. Chem.","firstPage":"40996","lastPage":"41002","volume":"278"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) IN COMPLEX WITH SUBSTRATE AND ATP ANALOG","SUBUNIT"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"U17224","properties":[{"key":"ProteinId","value":"AAD38229.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_110163358.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_QHMC01000009.1"}]},{"database":"PDB","id":"1Q15","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.30 A"},{"key":"Chains","value":"A/B/C/D=1-503"}]},{"database":"PDB","id":"1Q19","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.40 A"},{"key":"Chains","value":"A/B/C/D=1-503"}]},{"database":"PDBsum","id":"1Q15","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1Q19","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"Q9XB61","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9XB61","properties":[{"key":"Description","value":"-"}]},{"database":"DrugBank","id":"DB03215","properties":[{"key":"GenericName","value":"(2s,5s)-5-Carboxymethylproline"}]},{"database":"DrugBank","id":"DB02596","properties":[{"key":"GenericName","value":"alpha,beta-Methyleneadenosine 5'-triphosphate"}]},{"database":"KEGG","id":"ag:AAD38229","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:MONOMER-13572","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"6.3.3.6","properties":[{"key":"OrganismId","value":"2140"}]},{"database":"UniPathway","id":"UPA00182","properties":[{"key":"RectionId","value":"-"}]},{"database":"EvolutionaryTrace","id":"Q9XB61","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0004066","properties":[{"key":"GoTerm","value":"F:asparagine synthase (glutamine-hydrolyzing) activity"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"CDD","id":"cd01991","properties":[{"key":"EntryName","value":"Asn_synthase_B_C"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd01909","properties":[{"key":"EntryName","value":"betaLS_CarA_N"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.60.20.10","properties":[{"key":"EntryName","value":"Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.620","properties":[{"key":"EntryName","value":"HUPs"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR001962","properties":[{"key":"EntryName","value":"Asn_synthase"}]},{"database":"InterPro","id":"IPR050795","properties":[{"key":"EntryName","value":"Asn_Synthetase"}]},{"database":"InterPro","id":"IPR015230","properties":[{"key":"EntryName","value":"CarA_N"}]},{"database":"InterPro","id":"IPR029055","properties":[{"key":"EntryName","value":"Ntn_hydrolases_N"}]},{"database":"InterPro","id":"IPR014729","properties":[{"key":"EntryName","value":"Rossmann-like_a/b/a_fold"}]},{"database":"PANTHER","id":"PTHR11772","properties":[{"key":"EntryName","value":"ASPARAGINE SYNTHETASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11772:SF2","properties":[{"key":"EntryName","value":"ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING]"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00733","properties":[{"key":"EntryName","value":"Asn_synthase"},{"key":"MatchStatus","value":"2"}]},{"database":"Pfam","id":"PF09147","properties":[{"key":"EntryName","value":"CarA_N"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52402","properties":[{"key":"EntryName","value":"Adenine nucleotide alpha hydrolases-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF56235","properties":[{"key":"EntryName","value":"N-terminal nucleophile aminohydrolases (Ntn hydrolases)"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSNSFCVVYKGSDTDINNIQRDFDGKGEALSNGYLFIEQNGHYQKCEMERGTAYLIGSLYNRTFLIGLAGVWEGEAYLANDAELLALLFTRLGANALALAEGDFCFFIDEPNGELTVITESRGFSPVHVVQGKKAWMTNSLKLVTAAEGEGALWFEEEALVCQSLMRADTYTPVKNAQRLKPGAVHVLTHDSEGYSFVESRTLTTPASNQLLALPREPLLALIDRYLNAPLEDLAPRFDTVGIPLSGGLDSSLVTALASRHFKKLNTYSIGTELSNEFEFSQQVADALGTHHQMKILSETEVINGIIESIYYNEIFDGLSAEIQSGLFNVYRQAQGQVSCMLTGYGSDLLFGGILKPGAQYDNPNQLLAEQVYRTRWTGEFATHGASCYGIDIRHPFWSHSLISLCHALHPDYKIFDNEVKNILREYADSLQLLPKDIVWRKKIGIHEGSSVNQAFANVLGSTVDNYQTKSRFTYRVYQAFLRGRLSITDVTPSQLKDLIKKD","length":503,"molWeight":55998,"crc64":"3BB1DBBFF352DBCC","md5":"B1734733C6C7F646149CB6000FA501EB"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"BIOPHYSICOCHEMICAL PROPERTIES":1,"PATHWAY":1,"SUBUNIT":1},"countByFeatureType":{"Chain":1,"Domain":1,"Active site":2,"Binding site":8,"Mutagenesis":6,"Beta strand":18,"Helix":27,"Turn":3},"uniParcId":"UPI00000B56AA"}}