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Essential for maintenance of adult photoreceptor cells (PubMed:23226104). Upon light stimulation, required for trafficking of newly synthesized ninaE (Rh1) from the trans-Golgi network to rhabdomere membranes via Rab11-dependent vesicular transport (PubMed:23226104). During egg development, essential for establishing and maintaining epithelial cell polarity by regulating the correct polarized deposition of basal membrane (BM) proteins in follicular epithelial (FE) cells (PubMed:18331716, PubMed:23369713, PubMed:24828534). Functions by targeting Rab10 to the basal cytoplasm, where it restricts the secretion of BM proteins such as trol/Pcan and vkg/Coll IV to the basal surface (PubMed:18331716, PubMed:23369713, PubMed:24828534). Appears to be involved in regulating the levels and distribution of the guanine nucleotide exchange factor strat, however the two proteins appear to have independent roles in regulating polarized BM protein secretion in the FE (PubMed:28228250)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18331716"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23226104"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23369713"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9813038"}],"value":"Interacts with Cam (PubMed:18331716, PubMed:9813038). Interacts with Rab10 (PubMed:23369713). Interacts (via the DENN domains) with Rab11 (PubMed:23226104)"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"Q9W3D3","intActId":"EBI-868608"},"interactantTwo":{"uniProtKBAccession":"O18335","geneName":"Rab11","intActId":"EBI-148897"},"numberOfExperiments":2,"organismDiffer":false}]},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18331716"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23226104"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23369713"}],"value":"In follicle cells (FC), accumulates at the plasma membrane and in both early and recycling endosomes. In FCs, detected at the lateral and apical cell cortex during early stages of oogenesis. At later stages, mostly expressed at the lateral cortex but some expression is still detected at the apical surface (PubMed:18331716). Colocalizes with Rab10 near the FC basal surface (PubMed:23369713). In photoreceptor cells, colocalizes with Rab11 in punctate structures (PubMed:23226104)"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18331716"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23369713"}],"value":"Cytoplasm, cell cortex","id":"SL-0138"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18331716"}],"value":"Early endosome","id":"SL-0094"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18331716"}],"value":"Recycling endosome","id":"SL-0232"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23226104"}],"value":"Cytoplasmic granule","id":"SL-0281"}}]},{"commentType":"ALTERNATIVE PRODUCTS","events":["Alternative splicing"],"isoforms":[{"name":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"18331716"},{"evidenceCode":"ECO:0000312","source":"FlyBase","id":"FBgn0025864"}],"value":"A"},"synonyms":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"FlyBase","id":"FBgn0025864"}],"value":"D"}],"isoformIds":["Q9W3D3-1"],"isoformSequenceStatus":"Displayed"},{"name":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"18331716"},{"evidenceCode":"ECO:0000312","source":"FlyBase","id":"FBgn0025864"}],"value":"B"},"isoformIds":["Q9W3D3-2"],"sequenceIds":["VSP_059814","VSP_059815"],"isoformSequenceStatus":"Described"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9813038"}],"value":"Expressed in the adult head and body"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18331716"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9813038"}],"value":"Expressed in the follicle epithelial cells throughout their development (PubMed:18331716). Not detected until the adult stage (PubMed:9813038)"}],"commentType":"DEVELOPMENTAL STAGE"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Frameshift","sequence":"CAA76938.1","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1671,"modifier":"EXACT"}},"description":"DENN domain-containing protein Crag","featureId":"PRO_0000445077"},{"type":"Domain","location":{"start":{"value":39,"modifier":"EXACT"},"end":{"value":195,"modifier":"EXACT"}},"description":"MABP","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00831"}]},{"type":"Domain","location":{"start":{"value":187,"modifier":"EXACT"},"end":{"value":364,"modifier":"EXACT"}},"description":"uDENN","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00304"}]},{"type":"Domain","location":{"start":{"value":385,"modifier":"EXACT"},"end":{"value":521,"modifier":"EXACT"}},"description":"cDENN","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00304"}]},{"type":"Domain","location":{"start":{"value":523,"modifier":"EXACT"},"end":{"value":632,"modifier":"EXACT"}},"description":"dDENN","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00304"}]},{"type":"Region","location":{"start":{"value":997,"modifier":"EXACT"},"end":{"value":1160,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":1245,"modifier":"EXACT"},"end":{"value":1311,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":1415,"modifier":"EXACT"},"end":{"value":1435,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1011,"modifier":"EXACT"},"end":{"value":1023,"modifier":"EXACT"}},"description":"Acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1050,"modifier":"EXACT"},"end":{"value":1061,"modifier":"EXACT"}},"description":"Acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1072,"modifier":"EXACT"},"end":{"value":1089,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1100,"modifier":"EXACT"},"end":{"value":1119,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1136,"modifier":"EXACT"},"end":{"value":1147,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1254,"modifier":"EXACT"},"end":{"value":1277,"modifier":"EXACT"}},"description":"Basic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1281,"modifier":"EXACT"},"end":{"value":1301,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Alternative sequence","location":{"start":{"value":1180,"modifier":"EXACT"},"end":{"value":1206,"modifier":"EXACT"}},"description":"in isoform B","evidences":[{"evidenceCode":"ECO:0000305"}],"featureId":"VSP_059814","alternativeSequence":{}},{"type":"Alternative sequence","location":{"start":{"value":1431,"modifier":"EXACT"},"end":{"value":1435,"modifier":"EXACT"}},"description":"in isoform B","featureId":"VSP_059815","alternativeSequence":{"originalSequence":"IANGN","alternativeSequences":["TANGT"]}},{"type":"Mutagenesis","location":{"start":{"value":161,"modifier":"EXACT"},"end":{"value":1671,"modifier":"EXACT"}},"description":"In GG43; larvae lethal.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18331716"}],"alternativeSequence":{}},{"type":"Mutagenesis","location":{"start":{"value":367,"modifier":"EXACT"},"end":{"value":1671,"modifier":"EXACT"}},"description":"In CJ101; larvae lethal. In egg chambers, follicle cells (FC) display abnormal localization of various apical, junctional and basolateral proteins, and loss of epithelial integrity. In follicle epithelium (FE) cells, the basal membrane proteins, trol, Lam and vkg display abnormal localization; accumulating on the apical side of the cell in addition to the normal basal accumulation. Expression of strat in the FE is reduced. In egg chambers, trol also shows abnormal apical localization at the posterior pole. Many FCs appear irregularly shaped at the poles, form multiple layers and are often found in the germline cluster. FCs on the lateral side however, appear normal.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18331716"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24828534"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28228250"}],"alternativeSequence":{}},{"type":"Mutagenesis","location":{"start":{"value":659,"modifier":"EXACT"},"end":{"value":1671,"modifier":"EXACT"}},"description":"In CZ085; larvae lethal.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18331716"}],"alternativeSequence":{}},{"type":"Mutagenesis","location":{"start":{"value":798,"modifier":"EXACT"},"end":{"value":1671,"modifier":"EXACT"}},"description":"Larvae lethal at the L2 stage. In photoreceptor cells, light-induced accumulation of newly synthesized ninaE (Rh1) post-Golgi vesicles leads to a reduction in membrane stack volume, and eventually, photoreceptor cell degeneration. Disruption of photoreceptor cell function is light- and age-dependent.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23226104"}],"alternativeSequence":{}},{"type":"Mutagenesis","location":{"start":{"value":1372,"modifier":"EXACT"},"end":{"value":1372,"modifier":"EXACT"}},"description":"Larvae lethal at the L2 stage. 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