{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9VLT8","secondaryAccessions":["Q8SYM1"],"uniProtkbId":"WASC3_DROME","entryAudit":{"firstPublicDate":"2010-01-19","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2002-10-01","entryVersion":142,"sequenceVersion":2},"annotationScore":4.0,"organism":{"scientificName":"Drosophila melanogaster","commonName":"Fruit fly","taxonId":7227,"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Altocrustacea","Allotriocarida","Hexapoda","Insecta","Pterygota","Neoptera","Eumetabola","Endopterygota","Aparaglossata","Panorpida","Diptera","Brachycera","Muscomorpha","Ephydroidea","Drosophilidae","Drosophila","Sophophora"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9Y3C0"}],"value":"WASH complex subunit 3"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Coiled-coil domain-containing protein 53 homolog"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000312","source":"FlyBase","id":"FBgn0031979"}],"value":"CCDC53"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"FlyBase","id":"FBgn0031979"}],"value":"CG7429"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9Y3C0"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25739458"}],"value":"Acts at least in part as component of the WASH complex which may regulate wash nucleation-promoting factor (NPF) activity and is required for its membrane targeting during endosomal sorting (By similarity). During embryogenesis, not involved in the wash-dependent developmental migration of hemocytes anteriorly from the tail (PubMed:25739458)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20498093"}],"value":"Component of the WASH complex"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"Q9VLT8","intActId":"EBI-189750"},"interactantTwo":{"uniProtKBAccession":"Q9VK90","geneName":"BcDNA:RE36920","intActId":"EBI-106128"},"numberOfExperiments":3,"organismDiffer":false}]},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Early endosome","id":"SL-0094"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the CCDC53 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":176,"modifier":"EXACT"}},"description":"WASH complex subunit 3","featureId":"PRO_0000390958"},{"type":"Region","location":{"start":{"value":84,"modifier":"EXACT"},"end":{"value":123,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":152,"modifier":"EXACT"},"end":{"value":176,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Coiled coil","location":{"start":{"value":47,"modifier":"EXACT"},"end":{"value":74,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Compositional bias","location":{"start":{"value":104,"modifier":"EXACT"},"end":{"value":115,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]}],"keywords":[{"id":"KW-0175","category":"Domain","name":"Coiled coil"},{"id":"KW-0967","category":"Cellular component","name":"Endosome"},{"id":"KW-0653","category":"Biological process","name":"Protein transport"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"10731132","citationType":"journal article","authors":["Adams M.D.","Celniker S.E.","Holt R.A.","Evans C.A.","Gocayne J.D.","Amanatides P.G.","Scherer S.E.","Li P.W.","Hoskins R.A.","Galle R.F.","George R.A.","Lewis S.E.","Richards S.","Ashburner M.","Henderson S.N.","Sutton G.G.","Wortman J.R.","Yandell M.D.","Zhang Q.","Chen L.X.","Brandon R.C.","Rogers Y.-H.C.","Blazej R.G.","Champe M.","Pfeiffer B.D.","Wan K.H.","Doyle C.","Baxter E.G.","Helt G.","Nelson C.R.","Miklos G.L.G.","Abril J.F.","Agbayani A.","An H.-J.","Andrews-Pfannkoch C.","Baldwin D.","Ballew R.M.","Basu A.","Baxendale J.","Bayraktaroglu L.","Beasley E.M.","Beeson K.Y.","Benos P.V.","Berman B.P.","Bhandari D.","Bolshakov S.","Borkova D.","Botchan M.R.","Bouck J.","Brokstein P.","Brottier P.","Burtis K.C.","Busam D.A.","Butler H.","Cadieu E.","Center A.","Chandra I.","Cherry J.M.","Cawley S.","Dahlke C.","Davenport L.B.","Davies P.","de Pablos B.","Delcher A.","Deng Z.","Mays A.D.","Dew I.","Dietz S.M.","Dodson K.","Doup L.E.","Downes M.","Dugan-Rocha S.","Dunkov B.C.","Dunn P.","Durbin K.J.","Evangelista C.C.","Ferraz C.","Ferriera S.","Fleischmann W.","Fosler C.","Gabrielian A.E.","Garg N.S.","Gelbart W.M.","Glasser K.","Glodek A.","Gong F.","Gorrell J.H.","Gu Z.","Guan P.","Harris M.","Harris N.L.","Harvey D.A.","Heiman T.J.","Hernandez J.R.","Houck J.","Hostin D.","Houston K.A.","Howland T.J.","Wei M.-H.","Ibegwam C.","Jalali M.","Kalush F.","Karpen G.H.","Ke Z.","Kennison J.A.","Ketchum K.A.","Kimmel B.E.","Kodira C.D.","Kraft C.L.","Kravitz S.","Kulp D.","Lai Z.","Lasko P.","Lei Y.","Levitsky A.A.","Li J.H.","Li Z.","Liang Y.","Lin X.","Liu X.","Mattei B.","McIntosh T.C.","McLeod M.P.","McPherson D.","Merkulov G.","Milshina N.V.","Mobarry C.","Morris J.","Moshrefi A.","Mount S.M.","Moy M.","Murphy B.","Murphy L.","Muzny D.M.","Nelson D.L.","Nelson D.R.","Nelson K.A.","Nixon K.","Nusskern D.R.","Pacleb J.M.","Palazzolo M.","Pittman G.S.","Pan S.","Pollard J.","Puri V.","Reese M.G.","Reinert K.","Remington K.","Saunders R.D.C.","Scheeler F.","Shen H.","Shue B.C.","Siden-Kiamos I.","Simpson M.","Skupski M.P.","Smith T.J.","Spier E.","Spradling A.C.","Stapleton M.","Strong R.","Sun E.","Svirskas R.","Tector C.","Turner R.","Venter E.","Wang A.H.","Wang X.","Wang Z.-Y.","Wassarman D.A.","Weinstock G.M.","Weissenbach J.","Williams S.M.","Woodage T.","Worley K.C.","Wu D.","Yang S.","Yao Q.A.","Ye J.","Yeh R.-F.","Zaveri J.S.","Zhan M.","Zhang G.","Zhao Q.","Zheng L.","Zheng X.H.","Zhong F.N.","Zhong W.","Zhou X.","Zhu S.C.","Zhu X.","Smith H.O.","Gibbs R.A.","Myers E.W.","Rubin G.M.","Venter J.C."],"citationCrossReferences":[{"database":"PubMed","id":"10731132"},{"database":"DOI","id":"10.1126/science.287.5461.2185"}],"title":"The genome sequence of Drosophila melanogaster.","publicationDate":"2000","journal":"Science","firstPage":"2185","lastPage":"2195","volume":"287"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"Berkeley","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"12537572","citationType":"journal article","authors":["Misra S.","Crosby M.A.","Mungall C.J.","Matthews B.B.","Campbell K.S.","Hradecky P.","Huang Y.","Kaminker J.S.","Millburn G.H.","Prochnik S.E.","Smith C.D.","Tupy J.L.","Whitfield E.J.","Bayraktaroglu L.","Berman B.P.","Bettencourt B.R.","Celniker S.E.","de Grey A.D.N.J.","Drysdale R.A.","Harris N.L.","Richter J.","Russo S.","Schroeder A.J.","Shu S.Q.","Stapleton M.","Yamada C.","Ashburner M.","Gelbart W.M.","Rubin G.M.","Lewis S.E."],"citationCrossReferences":[{"database":"PubMed","id":"12537572"},{"database":"DOI","id":"10.1186/gb-2002-3-12-research0083"}],"title":"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review.","publicationDate":"2002","journal":"Genome Biol.","firstPage":"RESEARCH0083.1","lastPage":"RESEARCH0083.22","volume":"3"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"Berkeley","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"12537569","citationType":"journal article","authors":["Stapleton M.","Carlson J.W.","Brokstein P.","Yu C.","Champe M.","George R.A.","Guarin H.","Kronmiller B.","Pacleb J.M.","Park S.","Wan K.H.","Rubin G.M.","Celniker S.E."],"citationCrossReferences":[{"database":"PubMed","id":"12537569"},{"database":"DOI","id":"10.1186/gb-2002-3-12-research0080"}],"title":"A Drosophila full-length cDNA resource.","publicationDate":"2002","journal":"Genome Biol.","firstPage":"RESEARCH0080.1","lastPage":"RESEARCH0080.8","volume":"3"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"Berkeley","type":"STRAIN"},{"value":"Embryo","type":"TISSUE"}]},{"referenceNumber":4,"citation":{"id":"20498093","citationType":"journal article","authors":["Jia D.","Gomez T.S.","Metlagel Z.","Umetani J.","Otwinowski Z.","Rosen M.K.","Billadeau D.D."],"citationCrossReferences":[{"database":"PubMed","id":"20498093"},{"database":"DOI","id":"10.1073/pnas.0913293107"}],"title":"WASH and WAVE actin regulators of the Wiskott-Aldrich syndrome protein (WASP) family are controlled by analogous structurally related complexes.","publicationDate":"2010","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"10442","lastPage":"10447","volume":"107"},"referencePositions":["SUBUNIT"]},{"referenceNumber":5,"citation":{"id":"25739458","citationType":"journal article","authors":["Verboon J.M.","Rahe T.K.","Rodriguez-Mesa E.","Parkhurst S.M."],"citationCrossReferences":[{"database":"PubMed","id":"25739458"},{"database":"DOI","id":"10.1091/mbc.e14-08-1266"}],"title":"Wash functions downstream of Rho1 GTPase in a subset of Drosophila immune cell developmental migrations.","publicationDate":"2015","journal":"Mol. Biol. Cell","firstPage":"1665","lastPage":"1674","volume":"26"},"referencePositions":["FUNCTION"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AE014134","properties":[{"key":"ProteinId","value":"AAF52595.2"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AY071457","properties":[{"key":"ProteinId","value":"AAL49079.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"RefSeq","id":"NP_609178.1","properties":[{"key":"NucleotideSequenceId","value":"NM_135334.5"}]},{"database":"AlphaFoldDB","id":"Q9VLT8","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9VLT8","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"60231","properties":[{"key":"Interactions","value":"9"}]},{"database":"ComplexPortal","id":"CPX-2562","properties":[{"key":"EntryName","value":"WASH complex"}]},{"database":"FunCoup","id":"Q9VLT8","properties":[{"key":"Number of interactors","value":"1094"}]},{"database":"IntAct","id":"Q9VLT8","properties":[{"key":"Interactions","value":"4"}]},{"database":"STRING","id":"7227.FBpp0079162","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"7227-FBpp0079162","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"Q9VLT8","properties":[{"key":"Description","value":"-"}]},{"database":"DNASU","id":"34097","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblMetazoa","id":"FBtr0079540","properties":[{"key":"ProteinId","value":"FBpp0079162"},{"key":"GeneId","value":"FBgn0031979"}]},{"database":"GeneID","id":"34097","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"dme:Dmel_CG7429","properties":[{"key":"Description","value":"-"}]},{"database":"UCSC","id":"CG7429-RA","properties":[{"key":"OrganismName","value":"d. melanogaster"}]},{"database":"AGR","id":"FB:FBgn0031979","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"34097","properties":[{"key":"Description","value":"-"}]},{"database":"FlyBase","id":"FBgn0031979","properties":[{"key":"GeneName","value":"CCDC53"}]},{"database":"VEuPathDB","id":"VectorBase:FBgn0031979","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG4496","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_117940_1_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q9VLT8","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"GCETKFV","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"268027at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q9VLT8","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID-ORCS","id":"34097","properties":[{"key":"hits","value":"0 hits in 1 CRISPR screen"}]},{"database":"GenomeRNAi","id":"34097","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:Q9VLT8","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000803","properties":[{"key":"Component","value":"Chromosome 2L"}]},{"database":"Bgee","id":"FBgn0031979","properties":[{"key":"ExpressionPatterns","value":"Expressed in adult class III enteroendocrine cell in adult midgut (Drosophila) and 38 other cell types or tissues"}]},{"database":"GO","id":"GO:0031901","properties":[{"key":"GoTerm","value":"C:early endosome membrane"},{"key":"GoEvidenceType","value":"NAS:ComplexPortal"}],"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"20498093"}]},{"database":"GO","id":"GO:0005654","properties":[{"key":"GoTerm","value":"C:nucleoplasm"},{"key":"GoEvidenceType","value":"IDA:FlyBase"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"32503943"}]},{"database":"GO","id":"GO:0071203","properties":[{"key":"GoTerm","value":"C:WASH complex"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"20498093"}]},{"database":"GO","id":"GO:0030041","properties":[{"key":"GoTerm","value":"P:actin filament polymerization"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0032456","properties":[{"key":"GoTerm","value":"P:endocytic recycling"},{"key":"GoEvidenceType","value":"IMP:FlyBase"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"27884932"}]},{"database":"GO","id":"GO:0016197","properties":[{"key":"GoTerm","value":"P:endosomal transport"},{"key":"GoEvidenceType","value":"NAS:ComplexPortal"}],"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"20498093"}]},{"database":"GO","id":"GO:0006887","properties":[{"key":"GoTerm","value":"P:exocytosis"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0140591","properties":[{"key":"GoTerm","value":"P:nuclear envelope budding"},{"key":"GoEvidenceType","value":"IMP:FlyBase"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"32503943"}]},{"database":"GO","id":"GO:0034315","properties":[{"key":"GoTerm","value":"P:regulation of Arp2/3 complex-mediated actin nucleation"},{"key":"GoEvidenceType","value":"NAS:ComplexPortal"}],"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"20498093"}]},{"database":"Gene3D","id":"1.20.5.110","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR019309","properties":[{"key":"EntryName","value":"WASHC3"}]},{"database":"PANTHER","id":"PTHR13015","properties":[{"key":"EntryName","value":"PROTEIN AD-016-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR13015:SF0","properties":[{"key":"EntryName","value":"WASH COMPLEX SUBUNIT 3"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF10152","properties":[{"key":"EntryName","value":"CCDC53"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MDATAAITGNVDKTQIPPLNQKRILAFVNHFLVSTCTFLNEFALGCETKFVEMERQLQKTEAALIILEAKLASIPTEHHVATEATEAPAISNQQRNEEASMVDTTEPPTTENPTEPELPPESVGVRACEDQRYRKFFKMVQVGVPAPAVKQKMQSEGLEPRILDTPDLILADGQRE","length":176,"molWeight":19501,"crc64":"5F8EAA9E18263D63","md5":"BBE42D0F66CC996363DCA72E6C38A76A"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"INTERACTION":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Region":2,"Coiled coil":1,"Compositional bias":1},"uniParcId":"UPI000007BB5A"}}