{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9VHB9","uniProtkbId":"TRM44_DROME","entryAudit":{"firstPublicDate":"2006-09-19","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2009-07-07","entryVersion":129,"sequenceVersion":2},"annotationScore":2.0,"organism":{"scientificName":"Drosophila melanogaster","commonName":"Fruit fly","taxonId":7227,"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Altocrustacea","Allotriocarida","Hexapoda","Insecta","Pterygota","Neoptera","Eumetabola","Endopterygota","Aparaglossata","Panorpida","Diptera","Brachycera","Muscomorpha","Ephydroidea","Drosophilidae","Drosophila","Sophophora"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"value":"Probable tRNA (uracil-O(2)-)-methyltransferase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q02648"}],"value":"2.1.1.211"}]}},"genes":[{"geneName":{"value":"trmt44"},"orfNames":[{"value":"CG9386"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q02648"}],"value":"Probable adenosyl-L-methionine (AdoMet)-dependent tRNA (uracil-O(2)-)-methyltransferase"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"uridine(44) in tRNA(Ser) + S-adenosyl-L-methionine = 2'-O-methyluridine(44) in tRNA(Ser) + S-adenosyl-L-homocysteine + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:43100"},{"database":"Rhea","id":"RHEA-COMP:10339"},{"database":"Rhea","id":"RHEA-COMP:10340"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57856"},{"database":"ChEBI","id":"CHEBI:59789"},{"database":"ChEBI","id":"CHEBI:65315"},{"database":"ChEBI","id":"CHEBI:74478"}],"ecNumber":"2.1.1.211","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q02648"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q02648"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the TRM44 family"}],"commentType":"SIMILARITY"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"AAL48928.1","note":"Truncated N-terminus.","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":521,"modifier":"EXACT"}},"description":"Probable tRNA (uracil-O(2)-)-methyltransferase","featureId":"PRO_0000249899"}],"keywords":[{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0489","category":"Molecular function","name":"Methyltransferase"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0949","category":"Ligand","name":"S-adenosyl-L-methionine"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"id":"KW-0819","category":"Biological process","name":"tRNA processing"}],"references":[{"referenceNumber":1,"citation":{"id":"10731132","citationType":"journal article","authors":["Adams M.D.","Celniker S.E.","Holt R.A.","Evans C.A.","Gocayne J.D.","Amanatides P.G.","Scherer S.E.","Li P.W.","Hoskins R.A.","Galle R.F.","George R.A.","Lewis S.E.","Richards S.","Ashburner M.","Henderson S.N.","Sutton G.G.","Wortman J.R.","Yandell M.D.","Zhang Q.","Chen L.X.","Brandon R.C.","Rogers Y.-H.C.","Blazej R.G.","Champe M.","Pfeiffer B.D.","Wan K.H.","Doyle C.","Baxter E.G.","Helt G.","Nelson C.R.","Miklos G.L.G.","Abril J.F.","Agbayani A.","An H.-J.","Andrews-Pfannkoch C.","Baldwin D.","Ballew R.M.","Basu A.","Baxendale J.","Bayraktaroglu L.","Beasley E.M.","Beeson K.Y.","Benos P.V.","Berman B.P.","Bhandari D.","Bolshakov S.","Borkova D.","Botchan M.R.","Bouck J.","Brokstein P.","Brottier P.","Burtis K.C.","Busam D.A.","Butler H.","Cadieu E.","Center A.","Chandra I.","Cherry J.M.","Cawley S.","Dahlke C.","Davenport L.B.","Davies P.","de Pablos B.","Delcher A.","Deng Z.","Mays A.D.","Dew I.","Dietz S.M.","Dodson K.","Doup L.E.","Downes M.","Dugan-Rocha S.","Dunkov B.C.","Dunn P.","Durbin K.J.","Evangelista C.C.","Ferraz C.","Ferriera S.","Fleischmann W.","Fosler C.","Gabrielian A.E.","Garg N.S.","Gelbart W.M.","Glasser K.","Glodek A.","Gong F.","Gorrell J.H.","Gu Z.","Guan P.","Harris M.","Harris N.L.","Harvey D.A.","Heiman T.J.","Hernandez J.R.","Houck J.","Hostin D.","Houston K.A.","Howland T.J.","Wei M.-H.","Ibegwam C.","Jalali M.","Kalush F.","Karpen G.H.","Ke Z.","Kennison J.A.","Ketchum K.A.","Kimmel B.E.","Kodira C.D.","Kraft C.L.","Kravitz S.","Kulp D.","Lai Z.","Lasko P.","Lei Y.","Levitsky A.A.","Li J.H.","Li Z.","Liang Y.","Lin X.","Liu X.","Mattei B.","McIntosh T.C.","McLeod M.P.","McPherson D.","Merkulov G.","Milshina N.V.","Mobarry C.","Morris J.","Moshrefi A.","Mount S.M.","Moy M.","Murphy B.","Murphy L.","Muzny D.M.","Nelson D.L.","Nelson D.R.","Nelson K.A.","Nixon K.","Nusskern D.R.","Pacleb J.M.","Palazzolo M.","Pittman G.S.","Pan S.","Pollard J.","Puri V.","Reese M.G.","Reinert K.","Remington K.","Saunders R.D.C.","Scheeler F.","Shen H.","Shue B.C.","Siden-Kiamos I.","Simpson M.","Skupski M.P.","Smith T.J.","Spier E.","Spradling A.C.","Stapleton M.","Strong R.","Sun E.","Svirskas R.","Tector C.","Turner R.","Venter E.","Wang A.H.","Wang X.","Wang Z.-Y.","Wassarman D.A.","Weinstock G.M.","Weissenbach J.","Williams S.M.","Woodage T.","Worley K.C.","Wu D.","Yang S.","Yao Q.A.","Ye J.","Yeh R.-F.","Zaveri J.S.","Zhan M.","Zhang G.","Zhao Q.","Zheng L.","Zheng X.H.","Zhong F.N.","Zhong W.","Zhou X.","Zhu S.C.","Zhu X.","Smith H.O.","Gibbs R.A.","Myers E.W.","Rubin G.M.","Venter J.C."],"citationCrossReferences":[{"database":"PubMed","id":"10731132"},{"database":"DOI","id":"10.1126/science.287.5461.2185"}],"title":"The genome sequence of Drosophila melanogaster.","publicationDate":"2000","journal":"Science","firstPage":"2185","lastPage":"2195","volume":"287"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"Berkeley","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"12537572","citationType":"journal article","authors":["Misra S.","Crosby M.A.","Mungall C.J.","Matthews B.B.","Campbell K.S.","Hradecky P.","Huang Y.","Kaminker J.S.","Millburn G.H.","Prochnik S.E.","Smith C.D.","Tupy J.L.","Whitfield E.J.","Bayraktaroglu L.","Berman B.P.","Bettencourt B.R.","Celniker S.E.","de Grey A.D.N.J.","Drysdale R.A.","Harris N.L.","Richter J.","Russo S.","Schroeder A.J.","Shu S.Q.","Stapleton M.","Yamada C.","Ashburner M.","Gelbart W.M.","Rubin G.M.","Lewis S.E."],"citationCrossReferences":[{"database":"PubMed","id":"12537572"},{"database":"DOI","id":"10.1186/gb-2002-3-12-research0083"}],"title":"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review.","publicationDate":"2002","journal":"Genome Biol.","firstPage":"RESEARCH0083.1","lastPage":"RESEARCH0083.22","volume":"3"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"Berkeley","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"12537569","citationType":"journal article","authors":["Stapleton M.","Carlson J.W.","Brokstein P.","Yu C.","Champe M.","George R.A.","Guarin H.","Kronmiller B.","Pacleb J.M.","Park S.","Wan K.H.","Rubin G.M.","Celniker S.E."],"citationCrossReferences":[{"database":"PubMed","id":"12537569"},{"database":"DOI","id":"10.1186/gb-2002-3-12-research0080"}],"title":"A Drosophila full-length cDNA resource.","publicationDate":"2002","journal":"Genome Biol.","firstPage":"RESEARCH0080.1","lastPage":"RESEARCH0080.8","volume":"3"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4-521"],"referenceComments":[{"value":"Berkeley","type":"STRAIN"},{"value":"Embryo","type":"TISSUE"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AE014297","properties":[{"key":"ProteinId","value":"AAF54397.2"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AY071306","properties":[{"key":"ProteinId","value":"AAL48928.1"},{"key":"Status","value":"ALT_INIT"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"RefSeq","id":"NP_649904.2","properties":[{"key":"NucleotideSequenceId","value":"NM_141647.5"}]},{"database":"AlphaFoldDB","id":"Q9VHB9","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q9VHB9","properties":[{"key":"Number of interactors","value":"1519"}]},{"database":"STRING","id":"7227.FBpp0081593","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"7227-FBpp0081593","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"Q9VHB9","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblMetazoa","id":"FBtr0082115","properties":[{"key":"ProteinId","value":"FBpp0081593"},{"key":"GeneId","value":"FBgn0037708"}]},{"database":"GeneID","id":"41148","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"dme:Dmel_CG9386","properties":[{"key":"Description","value":"-"}]},{"database":"UCSC","id":"CG9386-RA","properties":[{"key":"OrganismName","value":"d. melanogaster"}]},{"database":"AGR","id":"FB:FBgn0037708","properties":[{"key":"Description","value":"-"}]},{"database":"FlyBase","id":"FBgn0037708","properties":[{"key":"GeneName","value":"CG9386"}]},{"database":"VEuPathDB","id":"VectorBase:FBgn0037708","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG3790","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_021025_1_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q9VHB9","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"CFFKLHH","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"10047021at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q9VHB9","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID-ORCS","id":"41148","properties":[{"key":"hits","value":"0 hits in 1 CRISPR screen"}]},{"database":"GenomeRNAi","id":"41148","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:Q9VHB9","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000803","properties":[{"key":"Component","value":"Chromosome 3R"}]},{"database":"Bgee","id":"FBgn0037708","properties":[{"key":"ExpressionPatterns","value":"Expressed in eye disc (Drosophila) and 43 other cell types or tissues"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0016300","properties":[{"key":"GoTerm","value":"F:tRNA (uridine) methyltransferase activity"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0141101","properties":[{"key":"GoTerm","value":"F:tRNA(Ser) (uridine(44)-2'-O-ribose)-methyltransferase activity"},{"key":"GoEvidenceType","value":"ISS:FlyBase"}]},{"database":"GO","id":"GO:0030488","properties":[{"key":"GoTerm","value":"P:tRNA methylation"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"InterPro","id":"IPR011671","properties":[{"key":"EntryName","value":"tRNA_uracil_MeTrfase"}]},{"database":"PANTHER","id":"PTHR21210","properties":[{"key":"EntryName","value":"TRNA (URACIL-O(2)-)-METHYLTRANSFERASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR21210:SF0","properties":[{"key":"EntryName","value":"TRNA (URACIL-O(2)-)-METHYLTRANSFERASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF07757","properties":[{"key":"EntryName","value":"AdoMet_MTase"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAAVEEAQFWQAIGILIKNYHALNKKIFEVVITQVTKQQNGRVCESSAEELAMSLKEDPSQRTCTGFTIGFKLLSKKLAENILGTGIVDFENCLYECQFASDSIEGFSVGLLGGEFKLKSKSNTNWLEFVLRPKLLSWSQSKQDEAKVKSLGLVNVEKYNDLYKELKQRHSQRLLEHWKTAQESTDPLKFIYEDLAIAAYLIVLWSQTQSEPTAFADLGCGNGLLVHVLNAEGYKGYGYDIRKRKLWSLYPPDTQRSLIEKAVEPNSFRLDFPGVDWLIGNHSDELSPWLPVLAGRLNINYFLLPCCPFELSGAKFRRRNTKISAYQDFFQYVTQVSHECGYEILQDRLKIPSTKRLALLGIKRKASKAIEDLEYFVQEELRKYKTGDAKIKLREKEESVRNCTQVDKTIIDGLVFKIFKLILDSNEDKWSGRLPMREIAQALTKEELSGIKSECGGIKTLLRNKHEVFEFCGGDLIGIRTPKPTATLPKSHLTIKKRSCFFKLHHPLGCPLDDAECSFIH","length":521,"molWeight":59384,"crc64":"E9E2D6111B8A3044","md5":"3A051C2E648DC44C02086227E70F3088"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"SEQUENCE CAUTION":1},"countByFeatureType":{"Chain":1},"uniParcId":"UPI00016DF7EC"}}