{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9UST7","uniProtkbId":"SFC3_SCHPO","entryAudit":{"firstPublicDate":"2007-10-23","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2000-05-01","entryVersion":142,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Schizosaccharomyces pombe (strain 972 / ATCC 24843)","commonName":"Fission yeast","taxonId":284812,"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Taphrinomycotina","Schizosaccharomycetes","Schizosaccharomycetales","Schizosaccharomycetaceae","Schizosaccharomyces"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Transcription factor tau subunit sfc3"}},"alternativeNames":[{"fullName":{"value":"TFIIIC subunit sfc3"}},{"fullName":{"value":"Transcription factor C subunit 3"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"CAB58159.1"}],"value":"sfc3"},"synonyms":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P34111"}],"value":"tfc3"}],"orfNames":[{"value":"SPBC336.07"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P34111"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10906331"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16751097"}],"value":"TFIIIC mediates tRNA and 5S RNA gene activation by binding to intragenic promoter elements. Upstream of the transcription start site, TFIIIC assembles the initiation complex TFIIIB-TFIIIC-tDNA, which is sufficient for RNA polymerase III recruitment and function. Part of the tauB domain of TFIIIC that binds boxB DNA promoter sites of tRNA and similar genes. Cooperates with sfc6 in DNA binding. Localizes to chromatin insulator sequence without recruiting RNA polymerase III and plays a role in nuclear organization"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P34111"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10906331"}],"value":"Component of the TFIIIC complex including sfc1, sfc3, sfc4, sfc6 and sfc7. The subunits are organized in two globular domains, tauA and tauB, connected by a proteolysis-sensitive and flexible linker. Interacts with sfc1, sfc4 and sfc6"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16823372"}],"value":"Nucleus envelope","id":"SL-0178"}}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1339,"modifier":"EXACT"}},"description":"Transcription factor tau subunit sfc3","featureId":"PRO_0000307794"},{"type":"DNA binding","location":{"start":{"value":485,"modifier":"EXACT"},"end":{"value":497,"modifier":"EXACT"}},"description":"A.T hook","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Region","location":{"start":{"value":470,"modifier":"EXACT"},"end":{"value":515,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":791,"modifier":"EXACT"},"end":{"value":826,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":495,"modifier":"EXACT"},"end":{"value":514,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":798,"modifier":"EXACT"},"end":{"value":826,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Modified residue","location":{"start":{"value":595,"modifier":"EXACT"},"end":{"value":595,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18257517"}]},{"type":"Modified residue","location":{"start":{"value":596,"modifier":"EXACT"},"end":{"value":596,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18257517"}]}],"keywords":[{"id":"KW-0238","category":"Molecular function","name":"DNA-binding"},{"id":"KW-0539","category":"Cellular component","name":"Nucleus"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0804","category":"Biological process","name":"Transcription"},{"id":"KW-0805","category":"Biological process","name":"Transcription regulation"}],"references":[{"referenceNumber":1,"citation":{"id":"11859360","citationType":"journal article","authors":["Wood V.","Gwilliam R.","Rajandream M.A.","Lyne M.H.","Lyne R.","Stewart A.","Sgouros J.G.","Peat N.","Hayles J.","Baker S.G.","Basham D.","Bowman S.","Brooks K.","Brown D.","Brown S.","Chillingworth T.","Churcher C.M.","Collins M.","Connor R.","Cronin A.","Davis P.","Feltwell T.","Fraser A.","Gentles S.","Goble A.","Hamlin N.","Harris D.E.","Hidalgo J.","Hodgson G.","Holroyd S.","Hornsby T.","Howarth S.","Huckle E.J.","Hunt S.","Jagels K.","James K.D.","Jones L.","Jones M.","Leather S.","McDonald S.","McLean J.","Mooney P.","Moule S.","Mungall K.L.","Murphy L.D.","Niblett D.","Odell C.","Oliver K.","O'Neil S.","Pearson D.","Quail M.A.","Rabbinowitsch E.","Rutherford K.M.","Rutter S.","Saunders D.","Seeger K.","Sharp S.","Skelton J.","Simmonds M.N.","Squares R.","Squares S.","Stevens K.","Taylor K.","Taylor R.G.","Tivey A.","Walsh S.V.","Warren T.","Whitehead S.","Woodward J.R.","Volckaert G.","Aert R.","Robben J.","Grymonprez B.","Weltjens I.","Vanstreels E.","Rieger M.","Schaefer M.","Mueller-Auer S.","Gabel C.","Fuchs M.","Duesterhoeft A.","Fritzc C.","Holzer E.","Moestl D.","Hilbert H.","Borzym K.","Langer I.","Beck A.","Lehrach H.","Reinhardt R.","Pohl T.M.","Eger P.","Zimmermann W.","Wedler H.","Wambutt R.","Purnelle B.","Goffeau A.","Cadieu E.","Dreano S.","Gloux S.","Lelaure V.","Mottier S.","Galibert F.","Aves S.J.","Xiang Z.","Hunt C.","Moore K.","Hurst S.M.","Lucas M.","Rochet M.","Gaillardin C.","Tallada V.A.","Garzon A.","Thode G.","Daga R.R.","Cruzado L.","Jimenez J.","Sanchez M.","del Rey F.","Benito J.","Dominguez A.","Revuelta J.L.","Moreno S.","Armstrong J.","Forsburg S.L.","Cerutti L.","Lowe T.","McCombie W.R.","Paulsen I.","Potashkin J.","Shpakovski G.V.","Ussery D.","Barrell B.G.","Nurse P."],"citationCrossReferences":[{"database":"PubMed","id":"11859360"},{"database":"DOI","id":"10.1038/nature724"}],"title":"The genome sequence of Schizosaccharomyces pombe.","publicationDate":"2002","journal":"Nature","firstPage":"871","lastPage":"880","volume":"415"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"972 / ATCC 24843","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"CAB58159.1"}]},{"referenceNumber":2,"citation":{"id":"10906331","citationType":"journal article","authors":["Huang Y.","Hamada M.","Maraia R.J."],"citationCrossReferences":[{"database":"PubMed","id":"10906331"},{"database":"DOI","id":"10.1074/jbc.m004635200"}],"title":"Isolation and cloning of four subunits of a fission yeast TFIIIC complex that includes an ortholog of the human regulatory protein TFIIICbeta.","publicationDate":"2000","journal":"J. Biol. Chem.","firstPage":"31480","lastPage":"31487","volume":"275"},"referencePositions":["FUNCTION","IDENTIFICATION IN TFIIIC","INTERACTION WITH SFC1; SFC4 AND SFC6"],"evidences":[{"evidenceCode":"ECO:0000305"}]},{"referenceNumber":3,"citation":{"id":"16751097","citationType":"journal article","authors":["Noma K.","Cam H.P.","Maraia R.J.","Grewal S.I.S."],"citationCrossReferences":[{"database":"PubMed","id":"16751097"},{"database":"DOI","id":"10.1016/j.cell.2006.04.028"}],"title":"A role for TFIIIC transcription factor complex in genome organization.","publicationDate":"2006","journal":"Cell","firstPage":"859","lastPage":"872","volume":"125"},"referencePositions":["FUNCTION"],"evidences":[{"evidenceCode":"ECO:0000305"}]},{"referenceNumber":4,"citation":{"id":"16823372","citationType":"journal article","authors":["Matsuyama A.","Arai R.","Yashiroda Y.","Shirai A.","Kamata A.","Sekido S.","Kobayashi Y.","Hashimoto A.","Hamamoto M.","Hiraoka Y.","Horinouchi S.","Yoshida M."],"citationCrossReferences":[{"database":"PubMed","id":"16823372"},{"database":"DOI","id":"10.1038/nbt1222"}],"title":"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.","publicationDate":"2006","journal":"Nat. Biotechnol.","firstPage":"841","lastPage":"847","volume":"24"},"referencePositions":["SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]"],"evidences":[{"evidenceCode":"ECO:0000305"}]},{"referenceNumber":5,"citation":{"id":"18257517","citationType":"journal article","authors":["Wilson-Grady J.T.","Villen J.","Gygi S.P."],"citationCrossReferences":[{"database":"PubMed","id":"18257517"},{"database":"DOI","id":"10.1021/pr7006335"}],"title":"Phosphoproteome analysis of fission yeast.","publicationDate":"2008","journal":"J. Proteome Res.","firstPage":"1088","lastPage":"1097","volume":"7"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-595 AND SER-596","IDENTIFICATION BY MASS SPECTROMETRY"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CU329671","properties":[{"key":"ProteinId","value":"CAB58159.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"T40245","properties":[{"key":"EntryName","value":"T40245"}]},{"database":"RefSeq","id":"NP_596127.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001022045.3"}]},{"database":"AlphaFoldDB","id":"Q9UST7","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9UST7","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"276778","properties":[{"key":"Interactions","value":"8"}]},{"database":"ComplexPortal","id":"CPX-8903","properties":[{"key":"EntryName","value":"General transcription factor TFIIIC complex"}]},{"database":"FunCoup","id":"Q9UST7","properties":[{"key":"Number of interactors","value":"6"}]},{"database":"IntAct","id":"Q9UST7","properties":[{"key":"Interactions","value":"3"}]},{"database":"STRING","id":"284812.Q9UST7","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"Q9UST7","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"284812-Q9UST7","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"2540246","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"spo:2540246","properties":[{"key":"Description","value":"-"}]},{"database":"PomBase","id":"SPBC336.07","properties":[{"key":"GeneName","value":"sfc3"}]},{"database":"VEuPathDB","id":"FungiDB:SPBC336.07","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"ENOG502S2X2","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_260174_0_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q9UST7","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"MSSMIQR","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"PhylomeDB","id":"Q9UST7","properties":[{"key":"Description","value":"-"}]},{"database":"Reactome","id":"R-SPO-76061","properties":[{"key":"PathwayName","value":"RNA Polymerase III Transcription Initiation From Type 1 Promoter"}]},{"database":"Reactome","id":"R-SPO-76066","properties":[{"key":"PathwayName","value":"RNA Polymerase III Transcription Initiation From Type 2 Promoter"}]},{"database":"PRO","id":"PR:Q9UST7","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000002485","properties":[{"key":"Component","value":"Chromosome II"}]},{"database":"GO","id":"GO:0005635","properties":[{"key":"GoTerm","value":"C:nuclear envelope"},{"key":"GoEvidenceType","value":"HDA:PomBase"}],"evidences":[{"evidenceCode":"ECO:0007005","source":"PubMed","id":"16823372"}]},{"database":"GO","id":"GO:0005634","properties":[{"key":"GoTerm","value":"C:nucleus"},{"key":"GoEvidenceType","value":"HDA:PomBase"}],"evidences":[{"evidenceCode":"ECO:0007005","source":"PubMed","id":"16823372"}]},{"database":"GO","id":"GO:0000127","properties":[{"key":"GoTerm","value":"C:transcription factor TFIIIC complex"},{"key":"GoEvidenceType","value":"IDA:PomBase"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"10906331"}]},{"database":"GO","id":"GO:0003677","properties":[{"key":"GoTerm","value":"F:DNA binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0000995","properties":[{"key":"GoTerm","value":"F:RNA polymerase III general transcription initiation factor activity"},{"key":"GoEvidenceType","value":"IDA:PomBase"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"10906331"}]},{"database":"GO","id":"GO:0042791","properties":[{"key":"GoTerm","value":"P:5S class rRNA transcription by RNA polymerase III"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0006384","properties":[{"key":"GoTerm","value":"P:transcription initiation at RNA polymerase III promoter"},{"key":"GoEvidenceType","value":"IDA:PomBase"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"10906331"}]},{"database":"InterPro","id":"IPR046488","properties":[{"key":"EntryName","value":"Sfc3/Tfc3_C"}]},{"database":"InterPro","id":"IPR044210","properties":[{"key":"EntryName","value":"Tfc3-like"}]},{"database":"InterPro","id":"IPR007309","properties":[{"key":"EntryName","value":"TFIIIC_Bblock-bd"}]},{"database":"InterPro","id":"IPR049543","properties":[{"key":"EntryName","value":"WHD_TFC3"}]},{"database":"PANTHER","id":"PTHR15180","properties":[{"key":"EntryName","value":"GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR15180:SF1","properties":[{"key":"EntryName","value":"GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04182","properties":[{"key":"EntryName","value":"B-block_TFIIIC"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF20222","properties":[{"key":"EntryName","value":"DUF6581"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF21552","properties":[{"key":"EntryName","value":"WHD_TFC3"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MDSLIRHCSEEIALDGSSGCSIDRLWEFAANFFFRQGIVQNLDDNYKTFVWPLILKEDGIEVWIEDEDATGLRSKMQEIPWNYQDITIELRARIRLFASEDRQWLTLTYKTKTDSKIQPLAFELLSCISRYRQEGVDRIQLCKETKQEPRSVYGRIQALEDASLISKVAINRSRAQTALLVLKRFESENSTINETVAQVSGKQIYNTEKIRSNICDAVQSSRNGICRHVDARAMVNLNHSRWERRYYARQVTYLHNNGYLRKCLTFVPNSPERCIRCLQFIKPYISSLDATEDDVDFTEVDDIPEDDELEEEEPLLPSKEEFDASFLQPLADVGPTLPQWSRFRPLEFQCFVLIRNAGFHGVITLQILSGLTGIRFNKPLFKLLGSLVEHRSSVPNHLSHMSITRFEDKTKKSRQYRYFTLQSQLNRLIRDGIPAETISKLLPHVHSLAGEFSPIDSSLFLNSLHHKGNMESNAEVSPDGMTLLPRKRGRPRKSANISVTSSPIRPSKNENNLPSLAISPVSEGFIQNATISTPSTSSNLSIAGSLTPSKTSRIYRGLAPLKNPEFNEDHVKKAEHLEPLLNVSSSVPSKNFTVSSPDHLKYGNTSSLQVSDSQSSIDLDSSFHYPVSVDSQLSHSTGSLMANFSSPTKKRRLESVDFIFLQRKGLILSYLVEQNGAFEISRKMFEDLADLKVRRNPETSRTVMDRRTFQQTLEKLMQEKKVRKLVIATNNGLGKLVRKDIVVQYDMKPDSPRFQQLRAQITTPEIEKQSTPEILKDVDVDFLKRNTSLSRRKSMPAEIKRHKESSETKPVDKEEVKKNEKEKDDPMRLAQQLLESLAPDFALHENTQQKSPVEKPKKLRKDRYASVEEFDYFSSTEHASKRSVKRFKNDFTSDEDETLIRAVVITQIYYGGTNRLIKWEAVQKCFPNRDIYALTRRYLSIRQHTKFKGLQQFLSENWQQMYKDAVSRKDLMPYPDSVDDFDPTPYVKASCRPYMISSSNLATTRLPRDLVDVYETYNIEVVKQETNFREMIFDPSLSVASKMNAYCDMPFTMPLSLNDKQNNEGDENCEKGQLFDAKSTIKSIVAIPDATYDARFSQERLMQYPEDILIAAHQELLDKRIITRVNSENSRLQPGRNFQFTEKFASSLKSPLPPFLLSQAKRFNKFLLEGFQNHKNHLFEETSNSGTLACILDLLSQGKLQISIVGSKFNEYGLSEGYRTRLLEHDNINVTLVLSGKESESKKNYGVTEKLATPPSHEPRLWLNGKCELIEMIWMNIEQSIVYQLLRKPGILRSQLTNLLFPGLEPREFNEVLDYFIAAGAAIEKDGLYLNHNYLFKLT","length":1339,"molWeight":153642,"crc64":"A05CA9F94180C562","md5":"37BE0C0F8C9427562E584B07142F6A61"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1},"countByFeatureType":{"Chain":1,"DNA binding":1,"Region":2,"Compositional bias":2,"Modified residue":2},"uniParcId":"UPI0000069A03"}}