{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9UJF2","secondaryAccessions":["F8W755","O95174","Q2TB22","Q5TFU9"],"uniProtkbId":"NGAP_HUMAN","entryAudit":{"firstPublicDate":"2001-05-04","lastAnnotationUpdateDate":"2026-06-10","lastSequenceUpdateDate":"2001-05-04","entryVersion":196,"sequenceVersion":2},"annotationScore":5.0,"organism":{"scientificName":"Homo sapiens","commonName":"Human","taxonId":9606,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Primates","Haplorrhini","Catarrhini","Hominidae","Homo"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Ras GTPase-activating protein nGAP"}},"alternativeNames":[{"fullName":{"value":"RAS protein activator-like 2"}}]},"genes":[{"geneName":{"value":"RASAL2"},"synonyms":[{"value":"NGAP"}]}],"comments":[{"texts":[{"value":"Inhibitory regulator of the Ras-cyclic AMP pathway"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35687021"}],"value":"Interacts with PEAK1"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"Q9UJF2","intActId":"EBI-359444"},"interactantTwo":{"uniProtKBAccession":"Q96PC5","geneName":"MIA2","intActId":"EBI-1050253"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q9UJF2","intActId":"EBI-359444"},"interactantTwo":{"uniProtKBAccession":"Q15653","geneName":"NFKBIB","intActId":"EBI-352889"},"numberOfExperiments":2,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q9UJF2","intActId":"EBI-359444"},"interactantTwo":{"uniProtKBAccession":"Q13077","geneName":"TRAF1","intActId":"EBI-359224"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q9UJF2","intActId":"EBI-359444"},"interactantTwo":{"uniProtKBAccession":"Q8N6Y0","geneName":"USHBP1","intActId":"EBI-739895"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q9UJF2","intActId":"EBI-359444"},"interactantTwo":{"uniProtKBAccession":"Q9WMX2","chainId":"PRO_0000037548","intActId":"EBI-6863741"},"numberOfExperiments":2,"organismDiffer":true},{"interactantOne":{"uniProtKBAccession":"Q9UJF2-2","intActId":"EBI-12171247"},"interactantTwo":{"uniProtKBAccession":"P23508","geneName":"MCC","intActId":"EBI-307531"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q9UJF2-2","intActId":"EBI-12171247"},"interactantTwo":{"uniProtKBAccession":"Q6FHY5","geneName":"MEOX2","intActId":"EBI-16439278"},"numberOfExperiments":3,"organismDiffer":false}]},{"commentType":"ALTERNATIVE PRODUCTS","events":["Alternative splicing"],"isoforms":[{"name":{"value":"1"},"isoformIds":["Q9UJF2-1"],"isoformSequenceStatus":"Displayed"},{"name":{"value":"2"},"isoformIds":["Q9UJF2-2"],"sequenceIds":["VSP_045777","VSP_045778"],"isoformSequenceStatus":"Described"}]},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"AAI10612.1","note":"Truncated N-terminus.","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1139,"modifier":"EXACT"}},"description":"Ras GTPase-activating protein nGAP","featureId":"PRO_0000056653"},{"type":"Domain","location":{"start":{"value":41,"modifier":"EXACT"},"end":{"value":158,"modifier":"EXACT"}},"description":"PH"},{"type":"Domain","location":{"start":{"value":149,"modifier":"EXACT"},"end":{"value":267,"modifier":"EXACT"}},"description":"C2","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00041"}]},{"type":"Domain","location":{"start":{"value":343,"modifier":"EXACT"},"end":{"value":551,"modifier":"EXACT"}},"description":"Ras-GAP","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00167"}]},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":87,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":684,"modifier":"EXACT"},"end":{"value":704,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":751,"modifier":"EXACT"},"end":{"value":782,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":803,"modifier":"EXACT"},"end":{"value":869,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":910,"modifier":"EXACT"},"end":{"value":953,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":1116,"modifier":"EXACT"},"end":{"value":1139,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":17,"modifier":"EXACT"},"end":{"value":36,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":45,"modifier":"EXACT"},"end":{"value":56,"modifier":"EXACT"}},"description":"Basic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":73,"modifier":"EXACT"},"end":{"value":87,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":751,"modifier":"EXACT"},"end":{"value":760,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":803,"modifier":"EXACT"},"end":{"value":818,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":833,"modifier":"EXACT"},"end":{"value":855,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":916,"modifier":"EXACT"},"end":{"value":928,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Site","location":{"start":{"value":369,"modifier":"EXACT"},"end":{"value":369,"modifier":"EXACT"}},"description":"Arginine finger; crucial for GTP hydrolysis by stabilizing the transition state","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00167"}]},{"type":"Modified residue","location":{"start":{"value":16,"modifier":"EXACT"},"end":{"value":16,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"18669648"},{"evidenceCode":"ECO:0007744","source":"PubMed","id":"24275569"}]},{"type":"Modified residue","location":{"start":{"value":89,"modifier":"EXACT"},"end":{"value":89,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"18669648"}]},{"type":"Modified residue","location":{"start":{"value":620,"modifier":"EXACT"},"end":{"value":620,"modifier":"EXACT"}},"description":"Phosphothreonine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"23186163"}]},{"type":"Modified residue","location":{"start":{"value":663,"modifier":"EXACT"},"end":{"value":663,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"18669648"},{"evidenceCode":"ECO:0007744","source":"PubMed","id":"23186163"}]},{"type":"Modified residue","location":{"start":{"value":864,"modifier":"EXACT"},"end":{"value":864,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"21406692"}]},{"type":"Alternative sequence","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":4,"modifier":"EXACT"}},"description":"in isoform 2","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"15489334"}],"featureId":"VSP_045777","alternativeSequence":{"originalSequence":"MQTP","alternativeSequences":["MELSPSSGGAAEALSWPEMFPALESDSPLPPEDLDAVVPVSGAVAGGMLDRILLESVCQQQSWVRVYDVKGPPTHRLSCGQSPYTETTTWERKYCILTDSQLVLLNKEKEIPVEGGQEQQTDSTKGRCLRRTVSVPSEGQFPEYPPEGATKL"]}},{"type":"Alternative sequence","location":{"start":{"value":590,"modifier":"EXACT"},"end":{"value":596,"modifier":"EXACT"}},"description":"in isoform 2","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"15489334"}],"featureId":"VSP_045778","alternativeSequence":{}},{"type":"Natural variant","location":{"start":{"value":165,"modifier":"EXACT"},"end":{"value":165,"modifier":"EXACT"}},"description":"in a colorectal cancer sample; somatic mutation; dbSNP:rs765646105","featureCrossReferences":[{"database":"dbSNP","id":"rs765646105"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16959974"}],"featureId":"VAR_035541","alternativeSequence":{"originalSequence":"R","alternativeSequences":["W"]}},{"type":"Natural variant","location":{"start":{"value":379,"modifier":"EXACT"},"end":{"value":379,"modifier":"EXACT"}},"description":"in a breast cancer sample; somatic mutation; dbSNP:rs1274912659","featureCrossReferences":[{"database":"dbSNP","id":"rs1274912659"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16959974"}],"featureId":"VAR_035542","alternativeSequence":{"originalSequence":"E","alternativeSequences":["D"]}}],"keywords":[{"id":"KW-0025","category":"Coding sequence diversity","name":"Alternative splicing"},{"id":"KW-0343","category":"Molecular function","name":"GTPase activation"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-1267","category":"Technical term","name":"Proteomics identification"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"9877179","citationType":"journal article","authors":["Noto S.","Maeda T.","Hattori S.","Inazawa J.","Imamura M.","Asaka M.","Hatakeyama M."],"citationCrossReferences":[{"database":"PubMed","id":"9877179"},{"database":"DOI","id":"10.1016/s0014-5793(98)01530-0"}],"title":"A novel human RasGAP-like gene that maps within the prostate cancer susceptibility locus at chromosome 1q25.","publicationDate":"1998","journal":"FEBS Lett.","firstPage":"127","lastPage":"131","volume":"441"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)"],"referenceComments":[{"value":"Heart","type":"TISSUE"}]},{"referenceNumber":2,"citation":{"id":"16710414","citationType":"journal article","authors":["Gregory S.G.","Barlow K.F.","McLay K.E.","Kaul R.","Swarbreck D.","Dunham A.","Scott C.E.","Howe K.L.","Woodfine K.","Spencer C.C.A.","Jones M.C.","Gillson C.","Searle S.","Zhou Y.","Kokocinski F.","McDonald L.","Evans R.","Phillips K.","Atkinson A.","Cooper R.","Jones C.","Hall R.E.","Andrews T.D.","Lloyd C.","Ainscough R.","Almeida J.P.","Ambrose K.D.","Anderson F.","Andrew R.W.","Ashwell R.I.S.","Aubin K.","Babbage A.K.","Bagguley C.L.","Bailey J.","Beasley H.","Bethel G.","Bird C.P.","Bray-Allen S.","Brown J.Y.","Brown A.J.","Buckley D.","Burton J.","Bye J.","Carder C.","Chapman J.C.","Clark S.Y.","Clarke G.","Clee C.","Cobley V.","Collier R.E.","Corby N.","Coville G.J.","Davies J.","Deadman R.","Dunn M.","Earthrowl M.","Ellington A.G.","Errington H.","Frankish A.","Frankland J.","French L.","Garner P.","Garnett J.","Gay L.","Ghori M.R.J.","Gibson R.","Gilby L.M.","Gillett W.","Glithero R.J.","Grafham D.V.","Griffiths C.","Griffiths-Jones S.","Grocock R.","Hammond S.","Harrison E.S.I.","Hart E.","Haugen E.","Heath P.D.","Holmes S.","Holt K.","Howden P.J.","Hunt A.R.","Hunt S.E.","Hunter G.","Isherwood J.","James R.","Johnson C.","Johnson D.","Joy A.","Kay M.","Kershaw J.K.","Kibukawa M.","Kimberley A.M.","King A.","Knights A.J.","Lad H.","Laird G.","Lawlor S.","Leongamornlert D.A.","Lloyd D.M.","Loveland J.","Lovell J.","Lush M.J.","Lyne R.","Martin S.","Mashreghi-Mohammadi M.","Matthews L.","Matthews N.S.W.","McLaren S.","Milne S.","Mistry S.","Moore M.J.F.","Nickerson T.","O'Dell C.N.","Oliver K.","Palmeiri A.","Palmer S.A.","Parker A.","Patel D.","Pearce A.V.","Peck A.I.","Pelan S.","Phelps K.","Phillimore B.J.","Plumb R.","Rajan J.","Raymond C.","Rouse G.","Saenphimmachak C.","Sehra H.K.","Sheridan E.","Shownkeen R.","Sims S.","Skuce C.D.","Smith M.","Steward C.","Subramanian S.","Sycamore N.","Tracey A.","Tromans A.","Van Helmond Z.","Wall M.","Wallis J.M.","White S.","Whitehead S.L.","Wilkinson J.E.","Willey D.L.","Williams H.","Wilming L.","Wray P.W.","Wu Z.","Coulson A.","Vaudin M.","Sulston J.E.","Durbin R.M.","Hubbard T.","Wooster R.","Dunham I.","Carter N.P.","McVean G.","Ross M.T.","Harrow J.","Olson M.V.","Beck S.","Rogers J.","Bentley D.R."],"citationCrossReferences":[{"database":"PubMed","id":"16710414"},{"database":"DOI","id":"10.1038/nature04727"}],"title":"The DNA sequence and biological annotation of human chromosome 1.","publicationDate":"2006","journal":"Nature","firstPage":"315","lastPage":"321","volume":"441"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"]},{"referenceNumber":3,"citation":{"id":"15489334","citationType":"journal article","authoringGroup":["The MGC Project Team"],"citationCrossReferences":[{"database":"PubMed","id":"15489334"},{"database":"DOI","id":"10.1101/gr.2596504"}],"title":"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).","publicationDate":"2004","journal":"Genome Res.","firstPage":"2121","lastPage":"2127","volume":"14"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-1139 (ISOFORM 2)"]},{"referenceNumber":4,"citation":{"id":"18669648","citationType":"journal article","authors":["Dephoure N.","Zhou C.","Villen J.","Beausoleil S.A.","Bakalarski C.E.","Elledge S.J.","Gygi S.P."],"citationCrossReferences":[{"database":"PubMed","id":"18669648"},{"database":"DOI","id":"10.1073/pnas.0805139105"}],"title":"A quantitative atlas of mitotic phosphorylation.","publicationDate":"2008","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"10762","lastPage":"10767","volume":"105"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16; SER-89 AND SER-663","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"Cervix carcinoma","type":"TISSUE"}]},{"referenceNumber":5,"citation":{"id":"19413330","citationType":"journal article","authors":["Gauci S.","Helbig A.O.","Slijper M.","Krijgsveld J.","Heck A.J.","Mohammed S."],"citationCrossReferences":[{"database":"PubMed","id":"19413330"},{"database":"DOI","id":"10.1021/ac9004309"}],"title":"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.","publicationDate":"2009","journal":"Anal. Chem.","firstPage":"4493","lastPage":"4501","volume":"81"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]},{"referenceNumber":6,"citation":{"id":"20068231","citationType":"journal article","authors":["Olsen J.V.","Vermeulen M.","Santamaria A.","Kumar C.","Miller M.L.","Jensen L.J.","Gnad F.","Cox J.","Jensen T.S.","Nigg E.A.","Brunak S.","Mann M."],"citationCrossReferences":[{"database":"PubMed","id":"20068231"},{"database":"DOI","id":"10.1126/scisignal.2000475"}],"title":"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.","publicationDate":"2010","journal":"Sci. Signal.","firstPage":"RA3","lastPage":"RA3","volume":"3"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"Cervix carcinoma","type":"TISSUE"}]},{"referenceNumber":7,"citation":{"id":"21269460","citationType":"journal article","authors":["Burkard T.R.","Planyavsky M.","Kaupe I.","Breitwieser F.P.","Buerckstuemmer T.","Bennett K.L.","Superti-Furga G.","Colinge J."],"citationCrossReferences":[{"database":"PubMed","id":"21269460"},{"database":"DOI","id":"10.1186/1752-0509-5-17"}],"title":"Initial characterization of the human central proteome.","publicationDate":"2011","journal":"BMC Syst. Biol.","firstPage":"17","lastPage":"17","volume":"5"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]},{"referenceNumber":8,"citation":{"id":"21406692","citationType":"journal article","authors":["Rigbolt K.T.","Prokhorova T.A.","Akimov V.","Henningsen J.","Johansen P.T.","Kratchmarova I.","Kassem M.","Mann M.","Olsen J.V.","Blagoev B."],"citationCrossReferences":[{"database":"PubMed","id":"21406692"},{"database":"DOI","id":"10.1126/scisignal.2001570"}],"title":"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.","publicationDate":"2011","journal":"Sci. Signal.","firstPage":"RS3","lastPage":"RS3","volume":"4"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-864","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]},{"referenceNumber":9,"citation":{"id":"23186163","citationType":"journal article","authors":["Zhou H.","Di Palma S.","Preisinger C.","Peng M.","Polat A.N.","Heck A.J.","Mohammed S."],"citationCrossReferences":[{"database":"PubMed","id":"23186163"},{"database":"DOI","id":"10.1021/pr300630k"}],"title":"Toward a comprehensive characterization of a human cancer cell phosphoproteome.","publicationDate":"2013","journal":"J. Proteome Res.","firstPage":"260","lastPage":"271","volume":"12"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-620 AND SER-663","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"Cervix carcinoma","type":"TISSUE"}]},{"referenceNumber":10,"citation":{"id":"24275569","citationType":"journal article","authors":["Bian Y.","Song C.","Cheng K.","Dong M.","Wang F.","Huang J.","Sun D.","Wang L.","Ye M.","Zou H."],"citationCrossReferences":[{"database":"PubMed","id":"24275569"},{"database":"DOI","id":"10.1016/j.jprot.2013.11.014"}],"title":"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.","publicationDate":"2014","journal":"J. Proteomics","firstPage":"253","lastPage":"262","volume":"96"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"Liver","type":"TISSUE"}]},{"referenceNumber":11,"citation":{"id":"35687021","citationType":"journal article","authors":["Zuidema A.","Atherton P.","Kreft M.","Hoekman L.","Bleijerveld O.B.","Nagaraj N.","Chen N.","Faessler R.","Sonnenberg A."],"citationCrossReferences":[{"database":"PubMed","id":"35687021"},{"database":"DOI","id":"10.1083/jcb.202108027"}],"title":"PEAK1 Y635 phosphorylation regulates cell migration through association with Tensin3 and integrins.","publicationDate":"2022","journal":"J. Cell Biol.","firstPage":"0","lastPage":"0","volume":"221"},"referencePositions":["INTERACTION WITH PEAK1"]},{"referenceNumber":12,"citation":{"id":"16959974","citationType":"journal article","authors":["Sjoeblom T.","Jones S.","Wood L.D.","Parsons D.W.","Lin J.","Barber T.D.","Mandelker D.","Leary R.J.","Ptak J.","Silliman N.","Szabo S.","Buckhaults P.","Farrell C.","Meeh P.","Markowitz S.D.","Willis J.","Dawson D.","Willson J.K.V.","Gazdar A.F.","Hartigan J.","Wu L.","Liu C.","Parmigiani G.","Park B.H.","Bachman K.E.","Papadopoulos N.","Vogelstein B.","Kinzler K.W.","Velculescu V.E."],"citationCrossReferences":[{"database":"PubMed","id":"16959974"},{"database":"DOI","id":"10.1126/science.1133427"}],"title":"The consensus coding sequences of human breast and colorectal cancers.","publicationDate":"2006","journal":"Science","firstPage":"268","lastPage":"274","volume":"314"},"referencePositions":["VARIANTS [LARGE SCALE ANALYSIS] TRP-165 AND ASP-379"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF047711","properties":[{"key":"ProteinId","value":"AAD04814.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AL035702","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AL365357","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AL160281","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AL499617","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"BC110611","properties":[{"key":"ProteinId","value":"AAI10612.1"},{"key":"Status","value":"ALT_INIT"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"CCDS","id":"CCDS1321.2","properties":[{"key":"Description","value":"-"}],"isoformId":"Q9UJF2-2"},{"database":"CCDS","id":"CCDS1322.1","properties":[{"key":"Description","value":"-"}],"isoformId":"Q9UJF2-1"},{"database":"RefSeq","id":"NP_004832.1","properties":[{"key":"NucleotideSequenceId","value":"NM_004841.5"}],"isoformId":"Q9UJF2-1"},{"database":"RefSeq","id":"NP_733793.2","properties":[{"key":"NucleotideSequenceId","value":"NM_170692.4"}],"isoformId":"Q9UJF2-2"},{"database":"AlphaFoldDB","id":"Q9UJF2","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9UJF2","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"114848","properties":[{"key":"Interactions","value":"168"}]},{"database":"CORUM","id":"Q9UJF2","properties":[{"key":"Description","value":"-"}]},{"database":"DIP","id":"DIP-27570N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q9UJF2","properties":[{"key":"Number of interactors","value":"1503"}]},{"database":"IntAct","id":"Q9UJF2","properties":[{"key":"Interactions","value":"49"}]},{"database":"MINT","id":"Q9UJF2","properties":[{"key":"Description","value":"-"}]},{"database":"NDEx","id":"IQUERY-CP-RASAL2","properties":[{"key":"Evidences","value":"2 NDEx IQuery Curated Pathways"}]},{"database":"STRING","id":"9606.ENSP00000356621","properties":[{"key":"Description","value":"-"}]},{"database":"GlyGen","id":"Q9UJF2","properties":[{"key":"glycosylation","value":"3 sites, 2 N-linked glycans (2 sites), 1 O-linked glycan (1 site)"}]},{"database":"iPTMnet","id":"Q9UJF2","properties":[{"key":"Description","value":"-"}]},{"database":"PhosphoSitePlus","id":"Q9UJF2","properties":[{"key":"Description","value":"-"}]},{"database":"BioMuta","id":"RASAL2","properties":[{"key":"Description","value":"-"}]},{"database":"DMDM","id":"13959419","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"Q9UJF2","properties":[{"key":"Description","value":"-"}]},{"database":"MassIVE","id":"Q9UJF2","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"9606-ENSP00000356621","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"Q9UJF2","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"29892","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"84621","properties":[{"key":"Description","value":"-"}],"isoformId":"Q9UJF2-1"},{"database":"Pumba","id":"Q9UJF2","properties":[{"key":"Description","value":"-"}]},{"database":"Antibodypedia","id":"20575","properties":[{"key":"antibodies","value":"153 antibodies from 30 providers"}]},{"database":"DNASU","id":"9462","properties":[{"key":"Description","value":"-"}]},{"database":"Ensembl","id":"ENST00000367649.8","properties":[{"key":"ProteinId","value":"ENSP00000356621.3"},{"key":"GeneId","value":"ENSG00000075391.19"}],"isoformId":"Q9UJF2-2"},{"database":"Ensembl","id":"ENST00000462775.5","properties":[{"key":"ProteinId","value":"ENSP00000420558.1"},{"key":"GeneId","value":"ENSG00000075391.19"}],"isoformId":"Q9UJF2-1"},{"database":"GeneID","id":"9462","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"hsa:9462","properties":[{"key":"Description","value":"-"}]},{"database":"MANE-Select","id":"ENST00000367649.8","properties":[{"key":"ProteinId","value":"ENSP00000356621.3"},{"key":"RefSeqNucleotideId","value":"NM_170692.4"},{"key":"RefSeqProteinId","value":"NP_733793.2"}],"isoformId":"Q9UJF2-2"},{"database":"UCSC","id":"uc001glq.4","properties":[{"key":"OrganismName","value":"human"}],"isoformId":"Q9UJF2-1"},{"database":"AGR","id":"HGNC:9874","properties":[{"key":"Description","value":"-"}]},{"database":"ClinPGx","id":"PA34235","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"9462","properties":[{"key":"Description","value":"-"}]},{"database":"DisGeNET","id":"9462","properties":[{"key":"Description","value":"-"}]},{"database":"GeneCards","id":"RASAL2","properties":[{"key":"Description","value":"-"}]},{"database":"HGNC","id":"HGNC:9874","properties":[{"key":"GeneName","value":"RASAL2"}]},{"database":"HPA","id":"ENSG00000075391","properties":[{"key":"ExpressionPatterns","value":"Low tissue specificity"}]},{"database":"MalaCards","id":"RASAL2","properties":[{"key":"Description","value":"-"}]},{"database":"MIM","id":"606136","properties":[{"key":"Type","value":"gene"}]},{"database":"OpenTargets","id":"ENSG00000075391","properties":[{"key":"Description","value":"-"}]},{"database":"VEuPathDB","id":"HostDB:ENSG00000075391","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG3508","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"GeneTree","id":"ENSGT00940000157702","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_001727_1_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q9UJF2","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"HAAQMDH","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"5572587at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PAN-GO","id":"Q9UJF2","properties":[{"key":"Number of GO annotations","value":"2 GO annotations based on evolutionary models"}]},{"database":"PhylomeDB","id":"Q9UJF2","properties":[{"key":"Description","value":"-"}]},{"database":"PathwayCommons","id":"Q9UJF2","properties":[{"key":"Description","value":"-"}]},{"database":"Reactome","id":"R-HSA-5658442","properties":[{"key":"PathwayName","value":"Regulation of RAS by GAPs"}]},{"database":"Reactome","id":"R-HSA-9696264","properties":[{"key":"PathwayName","value":"RND3 GTPase cycle"}]},{"database":"Reactome","id":"R-HSA-9696273","properties":[{"key":"PathwayName","value":"RND1 GTPase cycle"}]},{"database":"SignaLink","id":"Q9UJF2","properties":[{"key":"Description","value":"-"}]},{"database":"Agora","id":"ENSG00000075391","properties":[{"key":"NominatedTarget","value":"-"}]},{"database":"BioGRID-ORCS","id":"9462","properties":[{"key":"hits","value":"7 hits in 1154 CRISPR screens"}]},{"database":"CD-CODE","id":"FB4E32DD","properties":[{"key":"EntryName","value":"Presynaptic clusters and postsynaptic densities"}]},{"database":"ChiTaRS","id":"RASAL2","properties":[{"key":"OrganismName","value":"human"}]},{"database":"GeneWiki","id":"RASAL2","properties":[{"key":"Description","value":"-"}]},{"database":"GenomeRNAi","id":"9462","properties":[{"key":"Description","value":"-"}]},{"database":"Pharos","id":"Q9UJF2","properties":[{"key":"DevelopmentLevel","value":"Tbio"}]},{"database":"PRO","id":"PR:Q9UJF2","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000005640","properties":[{"key":"Component","value":"Chromosome 1"}]},{"database":"RNAct","id":"Q9UJF2","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"Bgee","id":"ENSG00000075391","properties":[{"key":"ExpressionPatterns","value":"Expressed in buccal mucosa cell and 179 other cell types or tissues"}]},{"database":"ExpressionAtlas","id":"Q9UJF2","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"TAS:Reactome"}]},{"database":"GO","id":"GO:0005096","properties":[{"key":"GoTerm","value":"F:GTPase activator activity"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0060612","properties":[{"key":"GoTerm","value":"P:adipose tissue development"},{"key":"GoEvidenceType","value":"IEA:Ensembl"}]},{"database":"GO","id":"GO:0010467","properties":[{"key":"GoTerm","value":"P:gene expression"},{"key":"GoEvidenceType","value":"IEA:Ensembl"}]},{"database":"GO","id":"GO:0035264","properties":[{"key":"GoTerm","value":"P:multicellular organism growth"},{"key":"GoEvidenceType","value":"IEA:Ensembl"}]},{"database":"GO","id":"GO:1902531","properties":[{"key":"GoTerm","value":"P:regulation of intracellular signal transduction"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:2000257","properties":[{"key":"GoTerm","value":"P:regulation of protein activation cascade"},{"key":"GoEvidenceType","value":"IEA:Ensembl"}]},{"database":"GO","id":"GO:0002021","properties":[{"key":"GoTerm","value":"P:response to dietary excess"},{"key":"GoEvidenceType","value":"IEA:Ensembl"}]},{"database":"GO","id":"GO:0009749","properties":[{"key":"GoTerm","value":"P:response to glucose"},{"key":"GoEvidenceType","value":"IEA:Ensembl"}]},{"database":"GO","id":"GO:0007165","properties":[{"key":"GoTerm","value":"P:signal transduction"},{"key":"GoEvidenceType","value":"TAS:ProtInc"}],"evidences":[{"evidenceCode":"ECO:0000304","source":"PubMed","id":"9877179"}]},{"database":"CDD","id":"cd04013","properties":[{"key":"EntryName","value":"C2_SynGAP_like"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd05136","properties":[{"key":"EntryName","value":"RasGAP_DAB2IP"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.506.10:FF:000001","properties":[{"key":"EntryName","value":"Ras GTPase-activating protein nGAP isoform 2"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.60.40.150:FF:000010","properties":[{"key":"EntryName","value":"Ras GTPase-activating protein nGAP isoform 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.60.40.150","properties":[{"key":"EntryName","value":"C2 domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.506.10","properties":[{"key":"EntryName","value":"GTPase Activation - p120gap, domain 1"},{"key":"MatchStatus","value":"2"}]},{"database":"InterPro","id":"IPR000008","properties":[{"key":"EntryName","value":"C2_dom"}]},{"database":"InterPro","id":"IPR035892","properties":[{"key":"EntryName","value":"C2_domain_sf"}]},{"database":"InterPro","id":"IPR021887","properties":[{"key":"EntryName","value":"DAB2P_C"}]},{"database":"InterPro","id":"IPR001849","properties":[{"key":"EntryName","value":"PH_domain"}]},{"database":"InterPro","id":"IPR039360","properties":[{"key":"EntryName","value":"Ras_GTPase"}]},{"database":"InterPro","id":"IPR023152","properties":[{"key":"EntryName","value":"RasGAP_CS"}]},{"database":"InterPro","id":"IPR001936","properties":[{"key":"EntryName","value":"RasGAP_dom"}]},{"database":"InterPro","id":"IPR008936","properties":[{"key":"EntryName","value":"Rho_GTPase_activation_prot"}]},{"database":"InterPro","id":"IPR057606","properties":[{"key":"EntryName","value":"SynGAP1-like_PH"}]},{"database":"PANTHER","id":"PTHR10194:SF52","properties":[{"key":"EntryName","value":"RAS GTPASE-ACTIVATING PROTEIN NGAP"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10194","properties":[{"key":"EntryName","value":"RAS GTPASE-ACTIVATING PROTEINS"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00168","properties":[{"key":"EntryName","value":"C2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF12004","properties":[{"key":"EntryName","value":"DAB2P_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF25321","properties":[{"key":"EntryName","value":"PH_RASGAP"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00616","properties":[{"key":"EntryName","value":"RasGAP"},{"key":"MatchStatus","value":"2"}]},{"database":"SMART","id":"SM00239","properties":[{"key":"EntryName","value":"C2"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00233","properties":[{"key":"EntryName","value":"PH"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00323","properties":[{"key":"EntryName","value":"RasGAP"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF49562","properties":[{"key":"EntryName","value":"C2 domain (Calcium/lipid-binding domain, CaLB)"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48350","properties":[{"key":"EntryName","value":"GTPase activation domain, GAP"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF50729","properties":[{"key":"EntryName","value":"PH domain-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50004","properties":[{"key":"EntryName","value":"C2"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00509","properties":[{"key":"EntryName","value":"RAS_GTPASE_ACTIV_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50018","properties":[{"key":"EntryName","value":"RAS_GTPASE_ACTIV_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MQTPEVPAERSPRRRSISGTSTSEKPNSMDTANTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRSTDDRSRGLPKLKESRSHESLLSPCSTVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFFSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELIDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDERESSLPNGRSVSLMDLQDTHAAQVEHASVMLDVPIRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGGLQPLSFQNPVYHLNNPIPAMPKASIDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKVDQGGLGARAKAPPSLPHSASLRSTGSMSVVSAALVAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNLDEAKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIVSLDSANTRLMSALTQVKERYSMQVRNGISPTNPTKLSITENGEFKNSSC","length":1139,"molWeight":128558,"crc64":"4A65C8243E1259A3","md5":"A34F7927C63BDB4230F6B1F5B7A06F3E"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"INTERACTION":7,"ALTERNATIVE PRODUCTS":2,"SEQUENCE CAUTION":1},"countByFeatureType":{"Chain":1,"Domain":3,"Region":6,"Compositional bias":7,"Site":1,"Modified residue":5,"Alternative sequence":2,"Natural variant":2},"uniParcId":"UPI000012FFF1"}}