{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9SZ15","uniProtkbId":"LIP5_ARATH","entryAudit":{"firstPublicDate":"2015-01-07","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2000-05-01","entryVersion":147,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Arabidopsis thaliana","commonName":"Mouse-ear cress","taxonId":3702,"evidences":[{"evidenceCode":"ECO:0000312","source":"Proteomes","id":"UP000006548"}],"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","rosids","malvids","Brassicales","Brassicaceae","Camelineae","Arabidopsis"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"17468262"}],"value":"Protein HOMOLOG OF MAMMALIAN LYST-INTERACTING PROTEIN 5"}},"alternativeNames":[{"fullName":{"value":"Protein EXTENSIN-LIKE"},"shortNames":[{"value":"EXT-LIKE"}]}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"17468262"}],"value":"LIP5"},"synonyms":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"24385429"}],"value":"VTA1"}],"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"Araport","id":"AT4G26750"}],"value":"At4g26750"}],"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"CAB36521.1"}],"value":"F10M23.90"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17468262"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25010425"}],"value":"Involved in the endosomal multivesicular bodies (MVB) pathway. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and are delivered to lysosomes enabling degradation of membrane proteins (By similarity). Thought to be a cofactor of SKD1/VPS4, which catalyzes the disassembly of membrane-associated ESCRT-III (PubMed:17468262). Target of pathogen-responsive mitogen-activated protein kinases (MPKs) that plays a critical role in plant basal resistance to Pseudomonas syringae in a SKD1-dependent manner by promoting multivesicular bodies (MVBs) trafficking upon plant infection (PubMed:25010425)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17468262"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19304934"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20663085"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24385429"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25010425"}],"value":"Homodimer (PubMed:20663085, PubMed:25010425). Interacts with SKD1/VPS4, VPS60-1, CHMP1A and CHMP1B (PubMed:17468262, PubMed:19304934, PubMed:20663085). Binds to PROS/At4g24370 (PubMed:24385429, PubMed:25010425). Interacts with MPK6 and MPK3 (PubMed:25010425)"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"Q9SZ15","intActId":"EBI-1606558"},"interactantTwo":{"uniProtKBAccession":"Q9ZNT0","geneName":"SKD1","intActId":"EBI-1606459"},"numberOfExperiments":2,"organismDiffer":false}]},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25010425"}],"value":"Localized in multivesicular body when associated with SKD1"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25010425"}],"value":"Cytoplasm","id":"SL-0086"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9NP79"}],"value":"Endosome membrane","id":"SL-0100"},"topology":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9NP79"}],"value":"Peripheral membrane protein","id":"SL-9903"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25010425"}],"value":"Nucleus","id":"SL-0191"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25010425"}],"value":"Endosome, multivesicular body","id":"SL-0174"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25010425"}],"value":"By Pseudomonas syringae pv. tomato strain DC3000 (PstDC3000)"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25010425"}],"value":"Phosphorylated by activated MPK6 and MPK3, this activation is required to trigger multivesicular bodies (MVBs) trafficking upon plant infection"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17468262"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25010425"}],"value":"No strong phenotypic alterations under normal growth conditions; slightly slow growth, slightly pale green and flat leaves, and reduced seed yields (PubMed:17468262, PubMed:25010425). Increased sensitivity to Pseudomonas syringae pv. tomato strain DC3000 (PstDC3000) (PubMed:25010425)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the VTA1 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Initiator methionine","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"Removed","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"22223895"}]},{"type":"Chain","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":421,"modifier":"EXACT"}},"description":"Protein HOMOLOG OF MAMMALIAN LYST-INTERACTING PROTEIN 5","featureId":"PRO_0000431529"},{"type":"Region","location":{"start":{"value":146,"modifier":"EXACT"},"end":{"value":374,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":165,"modifier":"EXACT"},"end":{"value":185,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":188,"modifier":"EXACT"},"end":{"value":207,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":245,"modifier":"EXACT"},"end":{"value":258,"modifier":"EXACT"}},"description":"Pro residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":278,"modifier":"EXACT"},"end":{"value":293,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":294,"modifier":"EXACT"},"end":{"value":308,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":317,"modifier":"EXACT"},"end":{"value":337,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Modified residue","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":2,"modifier":"EXACT"}},"description":"N-acetylserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"22223895"}]},{"type":"Mutagenesis","location":{"start":{"value":73,"modifier":"EXACT"},"end":{"value":73,"modifier":"EXACT"}},"description":"Loss of MK3/MK6-mediated phosphorylation; when associated with A-153; A-254; A-285; A-307 and A-323.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25010425"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":153,"modifier":"EXACT"},"end":{"value":153,"modifier":"EXACT"}},"description":"Loss of MK3/MK6-mediated phosphorylation; when associated with A-73; A-254; A-285; A-307 and A-323.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25010425"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":254,"modifier":"EXACT"},"end":{"value":254,"modifier":"EXACT"}},"description":"Loss of MK3/MK6-mediated phosphorylation; when associated with A-73; A-153; A-285; A-307 and A-323.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25010425"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":285,"modifier":"EXACT"},"end":{"value":285,"modifier":"EXACT"}},"description":"Loss of MK3/MK6-mediated phosphorylation; when associated with A-73; A-153; A-254; A-307 and A-323.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25010425"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":307,"modifier":"EXACT"},"end":{"value":307,"modifier":"EXACT"}},"description":"Loss of MK3/MK6-mediated phosphorylation; when associated with A-73; A-153; A-254; A-285 and A-323.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25010425"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":323,"modifier":"EXACT"},"end":{"value":323,"modifier":"EXACT"}},"description":"Loss of MK3/MK6-mediated phosphorylation; when associated with A-73; A-153; A-254; A-285 and A-307.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25010425"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":388,"modifier":"EXACT"},"end":{"value":388,"modifier":"EXACT"}},"description":"Reduced SKD1 interaction.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25010425"}],"alternativeSequence":{"originalSequence":"F","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":395,"modifier":"EXACT"},"end":{"value":395,"modifier":"EXACT"}},"description":"Loss of SKD1 interaction.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25010425"}],"alternativeSequence":{"originalSequence":"F","alternativeSequences":["A"]}}],"keywords":[{"id":"KW-0007","category":"PTM","name":"Acetylation"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0967","category":"Cellular component","name":"Endosome"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0539","category":"Cellular component","name":"Nucleus"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-0611","category":"Biological process","name":"Plant defense"},{"id":"KW-0653","category":"Biological process","name":"Protein transport"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"10617198","citationType":"journal article","authors":["Mayer K.F.X.","Schueller C.","Wambutt R.","Murphy G.","Volckaert G.","Pohl T.","Duesterhoeft A.","Stiekema W.","Entian K.-D.","Terryn N.","Harris B.","Ansorge W.","Brandt P.","Grivell L.A.","Rieger M.","Weichselgartner M.","de Simone V.","Obermaier B.","Mache R.","Mueller M.","Kreis M.","Delseny M.","Puigdomenech P.","Watson M.","Schmidtheini T.","Reichert B.","Portetelle D.","Perez-Alonso M.","Boutry M.","Bancroft I.","Vos P.","Hoheisel J.","Zimmermann W.","Wedler H.","Ridley P.","Langham S.-A.","McCullagh B.","Bilham L.","Robben J.","van der Schueren J.","Grymonprez B.","Chuang Y.-J.","Vandenbussche F.","Braeken M.","Weltjens I.","Voet M.","Bastiaens I.","Aert R.","Defoor E.","Weitzenegger T.","Bothe G.","Ramsperger U.","Hilbert H.","Braun M.","Holzer E.","Brandt A.","Peters S.","van Staveren M.","Dirkse W.","Mooijman P.","Klein Lankhorst R.","Rose M.","Hauf J.","Koetter P.","Berneiser S.","Hempel S.","Feldpausch M.","Lamberth S.","Van den Daele H.","De Keyser A.","Buysshaert C.","Gielen J.","Villarroel R.","De Clercq R.","van Montagu M.","Rogers J.","Cronin A.","Quail M.A.","Bray-Allen S.","Clark L.","Doggett J.","Hall S.","Kay M.","Lennard N.","McLay K.","Mayes R.","Pettett A.","Rajandream M.A.","Lyne M.","Benes V.","Rechmann S.","Borkova D.","Bloecker H.","Scharfe M.","Grimm M.","Loehnert T.-H.","Dose S.","de Haan M.","Maarse A.C.","Schaefer M.","Mueller-Auer S.","Gabel C.","Fuchs M.","Fartmann B.","Granderath K.","Dauner D.","Herzl A.","Neumann S.","Argiriou A.","Vitale D.","Liguori R.","Piravandi E.","Massenet O.","Quigley F.","Clabauld G.","Muendlein A.","Felber R.","Schnabl S.","Hiller R.","Schmidt W.","Lecharny A.","Aubourg S.","Chefdor F.","Cooke R.","Berger C.","Monfort A.","Casacuberta E.","Gibbons T.","Weber N.","Vandenbol M.","Bargues M.","Terol J.","Torres A.","Perez-Perez A.","Purnelle B.","Bent E.","Johnson S.","Tacon D.","Jesse T.","Heijnen L.","Schwarz S.","Scholler P.","Heber S.","Francs P.","Bielke C.","Frishman D.","Haase D.","Lemcke K.","Mewes H.-W.","Stocker S.","Zaccaria P.","Bevan M.","Wilson R.K.","de la Bastide M.","Habermann K.","Parnell L.","Dedhia N.","Gnoj L.","Schutz K.","Huang E.","Spiegel L.","Sekhon M.","Murray J.","Sheet P.","Cordes M.","Abu-Threideh J.","Stoneking T.","Kalicki J.","Graves T.","Harmon G.","Edwards J.","Latreille P.","Courtney L.","Cloud J.","Abbott A.","Scott K.","Johnson D.","Minx P.","Bentley D.","Fulton B.","Miller N.","Greco T.","Kemp K.","Kramer J.","Fulton L.","Mardis E.","Dante M.","Pepin K.","Hillier L.W.","Nelson J.","Spieth J.","Ryan E.","Andrews S.","Geisel C.","Layman D.","Du H.","Ali J.","Berghoff A.","Jones K.","Drone K.","Cotton M.","Joshu C.","Antonoiu B.","Zidanic M.","Strong C.","Sun H.","Lamar B.","Yordan C.","Ma P.","Zhong J.","Preston R.","Vil D.","Shekher M.","Matero A.","Shah R.","Swaby I.K.","O'Shaughnessy A.","Rodriguez M.","Hoffman J.","Till S.","Granat S.","Shohdy N.","Hasegawa A.","Hameed A.","Lodhi M.","Johnson A.","Chen E.","Marra M.A.","Martienssen R.","McCombie W.R."],"citationCrossReferences":[{"database":"PubMed","id":"10617198"},{"database":"DOI","id":"10.1038/47134"}],"title":"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.","publicationDate":"1999","journal":"Nature","firstPage":"769","lastPage":"777","volume":"402"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"27862469","citationType":"journal article","authors":["Cheng C.Y.","Krishnakumar V.","Chan A.P.","Thibaud-Nissen F.","Schobel S.","Town C.D."],"citationCrossReferences":[{"database":"PubMed","id":"27862469"},{"database":"DOI","id":"10.1111/tpj.13415"}],"title":"Araport11: a complete reannotation of the Arabidopsis thaliana reference genome.","publicationDate":"2017","journal":"Plant J.","firstPage":"789","lastPage":"804","volume":"89"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"CI-CI4AM1G71UC0S","citationType":"submission","authoringGroup":["The Arabidopsis Information Resource (TAIR)"],"title":"Community-consensus reference genome of Arabidopsis thaliana Col-0.","publicationDate":"FEB-2026","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"14593172","citationType":"journal article","authors":["Yamada K.","Lim J.","Dale J.M.","Chen H.","Shinn P.","Palm C.J.","Southwick A.M.","Wu H.C.","Kim C.J.","Nguyen M.","Pham P.K.","Cheuk R.F.","Karlin-Newmann G.","Liu S.X.","Lam B.","Sakano H.","Wu T.","Yu G.","Miranda M.","Quach H.L.","Tripp M.","Chang C.H.","Lee J.M.","Toriumi M.J.","Chan M.M.","Tang C.C.","Onodera C.S.","Deng J.M.","Akiyama K.","Ansari Y.","Arakawa T.","Banh J.","Banno F.","Bowser L.","Brooks S.Y.","Carninci P.","Chao Q.","Choy N.","Enju A.","Goldsmith A.D.","Gurjal M.","Hansen N.F.","Hayashizaki Y.","Johnson-Hopson C.","Hsuan V.W.","Iida K.","Karnes M.","Khan S.","Koesema E.","Ishida J.","Jiang P.X.","Jones T.","Kawai J.","Kamiya A.","Meyers C.","Nakajima M.","Narusaka M.","Seki M.","Sakurai T.","Satou M.","Tamse R.","Vaysberg M.","Wallender E.K.","Wong C.","Yamamura Y.","Yuan S.","Shinozaki K.","Davis R.W.","Theologis A.","Ecker J.R."],"citationCrossReferences":[{"database":"PubMed","id":"14593172"},{"database":"DOI","id":"10.1126/science.1088305"}],"title":"Empirical analysis of transcriptional activity in the Arabidopsis genome.","publicationDate":"2003","journal":"Science","firstPage":"842","lastPage":"846","volume":"302"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"17468262","citationType":"journal article","authors":["Haas T.J.","Sliwinski M.K.","Martinez D.E.","Preuss M.","Ebine K.","Ueda T.","Nielsen E.","Odorizzi G.","Otegui M.S."],"citationCrossReferences":[{"database":"PubMed","id":"17468262"},{"database":"DOI","id":"10.1105/tpc.106.049346"}],"title":"The Arabidopsis AAA ATPase SKD1 is involved in multivesicular endosome function and interacts with its positive regulator LYST-INTERACTING PROTEIN5.","publicationDate":"2007","journal":"Plant Cell","firstPage":"1295","lastPage":"1312","volume":"19"},"referencePositions":["FUNCTION","DISRUPTION PHENOTYPE","INTERACTION WITH SKD1/VPS4"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":6,"citation":{"id":"19304934","citationType":"journal article","authors":["Spitzer C.","Reyes F.C.","Buono R.","Sliwinski M.K.","Haas T.J.","Otegui M.S."],"citationCrossReferences":[{"database":"PubMed","id":"19304934"},{"database":"DOI","id":"10.1105/tpc.108.064865"}],"title":"The ESCRT-related CHMP1A and B proteins mediate multivesicular body sorting of auxin carriers in Arabidopsis and are required for plant development.","publicationDate":"2009","journal":"Plant Cell","firstPage":"749","lastPage":"766","volume":"21"},"referencePositions":["INTERACTION WITH CHMP1A"]},{"referenceNumber":7,"citation":{"id":"20663085","citationType":"journal article","authors":["Shahriari M.","Keshavaiah C.","Scheuring D.","Sabovljevic A.","Pimpl P.","Haeusler R.E.","Huelskamp M.","Schellmann S."],"citationCrossReferences":[{"database":"PubMed","id":"20663085"},{"database":"DOI","id":"10.1111/j.1365-313x.2010.04310.x"}],"title":"The AAA-type ATPase AtSKD1 contributes to vacuolar maintenance of Arabidopsis thaliana.","publicationDate":"2010","journal":"Plant J.","firstPage":"71","lastPage":"85","volume":"64"},"referencePositions":["HOMODIMER","INTERACTION WITH SKD1; CHMP1A; CHMP1B AND VPS60-1"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":8,"citation":{"id":"22223895","citationType":"journal article","authors":["Bienvenut W.V.","Sumpton D.","Martinez A.","Lilla S.","Espagne C.","Meinnel T.","Giglione C."],"citationCrossReferences":[{"database":"PubMed","id":"22223895"},{"database":"DOI","id":"10.1074/mcp.m111.015131"}],"title":"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features.","publicationDate":"2012","journal":"Mol. Cell. Proteomics","firstPage":"M111.015131","lastPage":"M111.015131","volume":"11"},"referencePositions":["ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2","CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS]","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]},{"referenceNumber":9,"citation":{"id":"24385429","citationType":"journal article","authors":["Reyes F.C.","Buono R.A.","Roschzttardtz H.","Di Rubbo S.","Yeun L.H.","Russinova E.","Otegui M.S."],"citationCrossReferences":[{"database":"PubMed","id":"24385429"},{"database":"DOI","id":"10.1074/jbc.m113.529685"}],"title":"A novel endosomal sorting complex required for transport (ESCRT) component in Arabidopsis thaliana controls cell expansion and development.","publicationDate":"2014","journal":"J. Biol. Chem.","firstPage":"4980","lastPage":"4988","volume":"289"},"referencePositions":["INTERACTION WITH PROS/AT4G24370"]},{"referenceNumber":10,"citation":{"id":"25010425","citationType":"journal article","authors":["Wang F.","Shang Y.","Fan B.","Yu J.-Q.","Chen Z."],"citationCrossReferences":[{"database":"PubMed","id":"25010425"},{"database":"DOI","id":"10.1371/journal.ppat.1004243"}],"title":"Arabidopsis LIP5, a positive regulator of multivesicular body biogenesis, is a critical target of pathogen-responsive MAPK cascade in plant basal defense.","publicationDate":"2014","journal":"PLoS Pathog.","firstPage":"E1004243","lastPage":"E1004243","volume":"10"},"referencePositions":["FUNCTION","DISRUPTION PHENOTYPE","MUTAGENESIS OF SER-73; THR-153; SER-254; SER-285; SER-307; SER-323; PHE-388 AND PHE-395","INTERACTION WITH SKD1; MPK6 AND MPK3","HOMODIMERIZATION","PHOSPHORYLATION BY MPK6 AND MPK3","INDUCTION BY PSEUDOMONAS SYRINGAE","SUBCELLULAR LOCATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AL035440","properties":[{"key":"ProteinId","value":"CAB36521.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AL161565","properties":[{"key":"ProteinId","value":"CAB79530.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP002687","properties":[{"key":"ProteinId","value":"AEE85248.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"OZ408686","properties":[{"key":"ProteinId","value":"CAO7048857.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AY048219","properties":[{"key":"ProteinId","value":"AAK82482.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AY094009","properties":[{"key":"ProteinId","value":"AAM16165.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"PIR","id":"T04798","properties":[{"key":"EntryName","value":"T04798"}]},{"database":"RefSeq","id":"NP_194405.1","properties":[{"key":"NucleotideSequenceId","value":"NM_118809.5"}]},{"database":"AlphaFoldDB","id":"Q9SZ15","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9SZ15","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q9SZ15","properties":[{"key":"Number of interactors","value":"3843"}]},{"database":"IntAct","id":"Q9SZ15","properties":[{"key":"Interactions","value":"3"}]},{"database":"STRING","id":"3702.Q9SZ15","properties":[{"key":"Description","value":"-"}]},{"database":"TCDB","id":"3.A.31.1.2","properties":[{"key":"FamilyName","value":"the endosomal sorting complexes required for transport iii (escrt-iii) family"}]},{"database":"GlyGen","id":"Q9SZ15","properties":[{"key":"glycosylation","value":"1 site"}]},{"database":"iPTMnet","id":"Q9SZ15","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"3702-Q9SZ15","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"238418","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"828782","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ath:AT4G26750","properties":[{"key":"Description","value":"-"}]},{"database":"Araport","id":"AT4G26750","properties":[{"key":"Description","value":"-"}]},{"database":"TAIR","id":"AT4G26750","properties":[{"key":"GeneName","value":"EXT-LIKE"}]},{"database":"eggNOG","id":"KOG0917","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_035765_0_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q9SZ15","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"VSAQTHF","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"PhylomeDB","id":"Q9SZ15","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:Q9SZ15","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000006548","properties":[{"key":"Component","value":"Chromosome 4"}]},{"database":"ExpressionAtlas","id":"Q9SZ15","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"25010425"}]},{"database":"GO","id":"GO:0010008","properties":[{"key":"GoTerm","value":"C:endosome membrane"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0005771","properties":[{"key":"GoTerm","value":"C:multivesicular body"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"25010425"}]},{"database":"GO","id":"GO:0005634","properties":[{"key":"GoTerm","value":"C:nucleus"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"25010425"}]},{"database":"GO","id":"GO:0042803","properties":[{"key":"GoTerm","value":"F:protein homodimerization activity"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"20663085"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"25010425"}]},{"database":"GO","id":"GO:0098542","properties":[{"key":"GoTerm","value":"P:defense response to other organism"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"25010425"}]},{"database":"GO","id":"GO:0016197","properties":[{"key":"GoTerm","value":"P:endosomal transport"},{"key":"GoEvidenceType","value":"IGI:TAIR"}],"evidences":[{"evidenceCode":"ECO:0000316","source":"PubMed","id":"34125207"}]},{"database":"GO","id":"GO:0032511","properties":[{"key":"GoTerm","value":"P:late endosome to vacuole transport via multivesicular body sorting pathway"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0080001","properties":[{"key":"GoTerm","value":"P:mucilage extrusion from seed coat"},{"key":"GoEvidenceType","value":"IMP:TAIR"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"26983994"}]},{"database":"GO","id":"GO:0009555","properties":[{"key":"GoTerm","value":"P:pollen development"},{"key":"GoEvidenceType","value":"IGI:TAIR"}],"evidences":[{"evidenceCode":"ECO:0000316","source":"PubMed","id":"34125207"}]},{"database":"GO","id":"GO:1900426","properties":[{"key":"GoTerm","value":"P:positive regulation of defense response to bacterium"},{"key":"GoEvidenceType","value":"IMP:TAIR"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"25010425"}]},{"database":"GO","id":"GO:1903335","properties":[{"key":"GoTerm","value":"P:regulation of vacuolar transport"},{"key":"GoEvidenceType","value":"IGI:TAIR"}],"evidences":[{"evidenceCode":"ECO:0000316","source":"PubMed","id":"29209342"}]},{"database":"GO","id":"GO:0016192","properties":[{"key":"GoTerm","value":"P:vesicle-mediated transport"},{"key":"GoEvidenceType","value":"IPI:TAIR"}],"evidences":[{"evidenceCode":"ECO:0000353","source":"PubMed","id":"17468262"}]},{"database":"Gene3D","id":"1.20.5.420","properties":[{"key":"EntryName","value":"Immunoglobulin FC, subunit C"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.25.40.270","properties":[{"key":"EntryName","value":"Vacuolar protein sorting-associated protein vta1"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR044538","properties":[{"key":"EntryName","value":"Vta1-like"}]},{"database":"InterPro","id":"IPR039431","properties":[{"key":"EntryName","value":"Vta1/CALS_N"}]},{"database":"InterPro","id":"IPR023175","properties":[{"key":"EntryName","value":"Vta1/CALS_N_sf"}]},{"database":"InterPro","id":"IPR041212","properties":[{"key":"EntryName","value":"Vta1_C"}]},{"database":"PANTHER","id":"PTHR46009","properties":[{"key":"EntryName","value":"VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VTA1 HOMOLOG"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46009:SF1","properties":[{"key":"EntryName","value":"VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VTA1 HOMOLOG"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04652","properties":[{"key":"EntryName","value":"Vta1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF18097","properties":[{"key":"EntryName","value":"Vta1_C"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSNPNEPARALLPYLQRADELQKHEPLVAYYCRLYAMERGLKIPQSERTKTTNSILMSLINQLEKDKKSLTLSPDDNMHVEGFALSVFAKADKQDRAGRADLGTAKTFYAASIFFEILSQFGPVPPDIEQKHKYAAWKAADIRKAIKEGRKPTPGDPVDDDTDLSIPSSGPSGSYDHSASDTNTTSHHRTELDPPHDSNDDSSHHQFPEVPQHPLPPRFYDNPTNDYPADVPPPPPSSYPSNDHLPPPTGPSDSPYPHPYSHQPYHQDPPKHMPPPQNYSSHEPSPNSLPNFQSYPSFSESSLPSTSPHYPSHYQNPEPYYSSPHSAPAPSSTSFSSAPPPPPYSSNGRINIAPVLDPAPSSAQKYHYDSSYQPGPEKVAEALKAARFAVGALAFDEVSTAVEHLKKSLELLTNPSAGAGH","length":421,"molWeight":46218,"crc64":"1BD515B10FA4FA77","md5":"8D4A0F16FF4E12EACA28634A51596803"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"INTERACTION":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1,"PTM":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1},"countByFeatureType":{"Initiator methionine":1,"Chain":1,"Region":1,"Compositional bias":6,"Modified residue":1,"Mutagenesis":8},"uniParcId":"UPI00000A5231"}}