{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9R210","uniProtkbId":"TFEB_MOUSE","entryAudit":{"firstPublicDate":"2001-08-29","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2001-08-29","entryVersion":174,"sequenceVersion":2},"annotationScore":5.0,"organism":{"scientificName":"Mus musculus","commonName":"Mouse","taxonId":10090,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Glires","Rodentia","Myomorpha","Muroidea","Muridae","Murinae","Mus","Mus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"10036191"}],"value":"Transcription factor EB"}}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"10036191"},{"evidenceCode":"ECO:0000312","source":"MGI","id":"MGI:103270"}],"value":"Tfeb"},"synonyms":[{"value":"Tcfeb"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P19484"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16936731"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22343943"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24882217"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27184844"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27278822"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29146937"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35662396"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9806910"}],"value":"Transcription factor that acts as a master regulator of lysosomal biogenesis, autophagy, lysosomal exocytosis, lipid catabolism, energy metabolism and immune response (PubMed:16936731, PubMed:22343943, PubMed:27278822, PubMed:35662396). Specifically recognizes and binds E-box sequences (5'-CANNTG-3'); efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF (PubMed:16936731, PubMed:27278822). Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, TFEB phosphorylation by MTOR promotes its cytosolic retention and subsequent inactivation (PubMed:35662396). Upon starvation or lysosomal stress, inhibition of MTOR induces TFEB dephosphorylation, resulting in nuclear localization and transcription factor activity (By similarity). Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression, thereby playing a central role in expression of lysosomal genes (By similarity). Regulates lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1 (PubMed:29146937). Acts as a positive regulator of autophagy by promoting expression of genes involved in autophagy (PubMed:27278822). In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity (PubMed:16936731). Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer (By similarity). Plays a role in the signal transduction processes required for normal vascularization of the placenta (PubMed:9806910). Involved in the immune response to infection by the bacteria S.aureus, S.typhimurium or S.enterica (PubMed:24882217, PubMed:27184844, PubMed:35662396). Infection promotes itaconate production, leading to alkylation, resulting in nuclear localization and transcription factor activity (PubMed:35662396). Itaconate-mediated alkylation activates TFEB-dependent lysosomal biogenesis, facilitating the bacteria clearance during the antibacterial innate immune response (PubMed:35662396). In association with ACSS2, promotes the expression of genes involved in lysosome biogenesis and both autophagy upon glucose deprivation (By similarity)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P19484"}],"value":"Homodimer and heterodimer; with TFE3 or MITF (By similarity). Interacts (when phosphorylated by MTOR) with YWHAZ; promoting retention in the cytosol (By similarity). Interacts with Irgm1; promoting association between TFEB and PPP3CB and dephosphorylation (By similarity). Interacts with small GTPases Rag (RagA/RRAGA, RagB/RRAGB, RagC/RRAGC and/or RagD/RRAGD); promoting its recruitment to lysosomal membrane in the presence of nutrients (By similarity). Interacts with ACSS2 (By similarity)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P19484"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27278822"}],"value":"Mainly present in the cytoplasm (By similarity). When nutrients are present, recruited to the lysosomal membrane via association with GDP-bound RagC/RRAGC (or RagD/RRAGD): it is then phosphorylated by MTOR (By similarity). Phosphorylation by MTOR prevents nuclear translocation and activity by promoting interaction with 14-3-3 proteins, such as YWHAZ (By similarity). Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (By similarity). The translocation to the nucleus is regulated by ATP13A2 (PubMed:27278822). Conversely, inhibition of mTORC1, starvation and lysosomal disruption, promotes dephosphorylation and translocation to the nucleus (By similarity). Exported from the nucleus in response to nutrient availability (By similarity). In macrophages, translocates into the nucleus upon live S.enterica infection (By similarity)"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27278822"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32612235"}],"value":"Nucleus","id":"SL-0191"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P19484"}],"value":"Cytoplasm, cytosol","id":"SL-0091"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P19484"}],"value":"Lysosome membrane","id":"SL-0157"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16936731"}],"value":"Widely expressed"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9806910"}],"value":"Expressed at low levels in the embryo but at high levels in the labyrinthine trophoblast cells of the placenta"}],"commentType":"DEVELOPMENTAL STAGE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P19484"}],"value":"The leucine zipper region is essential for homo- or heterodimerization and high-affinity DNA binding. DNA binding is mediated by the basic region"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P19484"}],"value":"Phosphorylation at Ser-210 by MTOR via non-canonical mTORC1 pathway regulates its subcellular location and activity (By similarity). When nutrients are present, phosphorylation by MTOR promotes association with 14-3-3/YWHA adapters and retention in the cytosol (By similarity). Inhibition of mTORC1, starvation and lysosomal disruption, promotes dephosphorylation by calcineurin PPP3CB and translocation to the nucleus (By similarity). Dephosphorylated by calcineurin PPP3CB in response to lysosomal Ca(2+) release (By similarity). Irgm1 promotes dephosphorylation by calcineurin PPP3CB, resulting in TFEB nuclear translocation and stimulation of lysosomal biogenesis (By similarity). Exported from the nucleus in a mTORC1-dependent manner in response to nutrient availability (By similarity)"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35662396"}],"value":"Alkylated via a non-enzymatic covalent modification (PubMed:35662396). Itaconate, an anti-inflammatory metabolite generated in response to lipopolysaccharide, alkylates Cys-211, preventing association with 14-3-3/YWHA adapters, thereby promoting nuclear translocation and activity (PubMed:35662396)"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P19484"}],"value":"Sumoylated; does not affect dimerization with MITF"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32612235"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9806910"}],"value":"Death between 9.5 and 10.5 days in embryonic development (PubMed:9806910). Embryos display severe defects in placental vascularization (PubMed:9806910). The embryonic vasculature forms normally but few vessels are seen entering the placenta and those that do enter fail to thrive and branch normally (PubMed:9806910). Conditional deletion in kidney does not lead to any abnormality in the kidney (PubMed:32612235). Mice lacking Tfeb and Flcn in the kidney do not show any abnormality in the kidney, suggesting that the kidney phenotype observed in Flcn knockout mice is due to constitutive activation of Tfeb (PubMed:32612235)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the MiT/TFE family"}],"commentType":"SIMILARITY"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"AAD20979.1","note":"Extended N-terminus.","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":475,"modifier":"EXACT"}},"description":"Transcription factor EB","featureId":"PRO_0000127474"},{"type":"Domain","location":{"start":{"value":234,"modifier":"EXACT"},"end":{"value":287,"modifier":"EXACT"}},"description":"bHLH","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00981"}]},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":166,"modifier":"EXACT"}},"description":"Interaction with ACSS2","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P19484"}]},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":52,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":155,"modifier":"EXACT"},"end":{"value":164,"modifier":"EXACT"}},"description":"Strong transcription activation domain","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Region","location":{"start":{"value":297,"modifier":"EXACT"},"end":{"value":318,"modifier":"EXACT"}},"description":"Leucine-zipper"},{"type":"Region","location":{"start":{"value":351,"modifier":"EXACT"},"end":{"value":429,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":445,"modifier":"EXACT"},"end":{"value":475,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Motif","location":{"start":{"value":135,"modifier":"EXACT"},"end":{"value":152,"modifier":"EXACT"}},"description":"Nuclear export signal","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P19484"}]},{"type":"Motif","location":{"start":{"value":244,"modifier":"EXACT"},"end":{"value":247,"modifier":"EXACT"}},"description":"Nuclear localization signal","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P19484"}]},{"type":"Compositional bias","location":{"start":{"value":26,"modifier":"EXACT"},"end":{"value":43,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":380,"modifier":"EXACT"},"end":{"value":389,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":445,"modifier":"EXACT"},"end":{"value":468,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Modified residue","location":{"start":{"value":108,"modifier":"EXACT"},"end":{"value":108,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"21183079"}]},{"type":"Modified residue","location":{"start":{"value":113,"modifier":"EXACT"},"end":{"value":113,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"21183079"}]},{"type":"Modified residue","location":{"start":{"value":121,"modifier":"EXACT"},"end":{"value":121,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"21183079"}]},{"type":"Modified residue","location":{"start":{"value":137,"modifier":"EXACT"},"end":{"value":137,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"21183079"}]},{"type":"Modified residue","location":{"start":{"value":141,"modifier":"EXACT"},"end":{"value":141,"modifier":"EXACT"}},"description":"Phosphoserine; by MTOR","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"21183079"}]},{"type":"Modified residue","location":{"start":{"value":182,"modifier":"EXACT"},"end":{"value":182,"modifier":"EXACT"}},"description":"Phosphothreonine","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P19484"}]},{"type":"Modified residue","location":{"start":{"value":210,"modifier":"EXACT"},"end":{"value":210,"modifier":"EXACT"}},"description":"Phosphoserine; by MTOR","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P19484"}]},{"type":"Modified residue","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"S-(2,3-dicarboxypropyl)cysteine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35662396"}]},{"type":"Modified residue","location":{"start":{"value":331,"modifier":"EXACT"},"end":{"value":331,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P19484"}]},{"type":"Modified residue","location":{"start":{"value":422,"modifier":"EXACT"},"end":{"value":422,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P19484"}]},{"type":"Modified residue","location":{"start":{"value":440,"modifier":"EXACT"},"end":{"value":440,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P19484"}]},{"type":"Modified residue","location":{"start":{"value":465,"modifier":"EXACT"},"end":{"value":465,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"21183079"}]},{"type":"Modified residue","location":{"start":{"value":466,"modifier":"EXACT"},"end":{"value":466,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"21183079"}]},{"type":"Modified residue","location":{"start":{"value":468,"modifier":"EXACT"},"end":{"value":468,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"21183079"}]},{"type":"Mutagenesis","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"Abolished alkylation in knockin mice. Mice display elevated susceptibility to S.typhimurium infection.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35662396"}],"alternativeSequence":{"originalSequence":"C","alternativeSequences":["S"]}}],"keywords":[{"id":"KW-0010","category":"Molecular function","name":"Activator"},{"id":"KW-1064","category":"Biological process","name":"Adaptive immunity"},{"id":"KW-0072","category":"Biological process","name":"Autophagy"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0238","category":"Molecular function","name":"DNA-binding"},{"id":"KW-0391","category":"Biological process","name":"Immunity"},{"id":"KW-0458","category":"Cellular component","name":"Lysosome"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0539","category":"Cellular component","name":"Nucleus"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0804","category":"Biological process","name":"Transcription"},{"id":"KW-0805","category":"Biological process","name":"Transcription regulation"},{"id":"KW-0832","category":"PTM","name":"Ubl conjugation"}],"references":[{"referenceNumber":1,"citation":{"id":"10036191","citationType":"journal article","authors":["Rehli M.","Den Elzen N.","Cassady A.I.","Ostrowski M.C.","Hume D.A."],"citationCrossReferences":[{"database":"PubMed","id":"10036191"},{"database":"DOI","id":"10.1006/geno.1998.5588"}],"title":"Cloning and characterization of the murine genes for bHLH-ZIP transcription factors TFEC and TFEB reveal a common gene organization for all MiT subfamily members.","publicationDate":"1999","journal":"Genomics","firstPage":"111","lastPage":"120","volume":"56"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]"],"referenceComments":[{"value":"BALB/cJ","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9806910","citationType":"journal article","authors":["Steingrimsson E.","Tessarollo L.","Reid S.W.","Jenkins N.A.","Copeland N.G."],"citationCrossReferences":[{"database":"PubMed","id":"9806910"},{"database":"DOI","id":"10.1242/dev.125.23.4607"}],"title":"The bHLH-Zip transcription factor Tfeb is essential for placental vascularization.","publicationDate":"1998","journal":"Development","firstPage":"4607","lastPage":"4616","volume":"125"},"referencePositions":["FUNCTION","DISRUPTION PHENOTYPE","DEVELOPMENTAL STAGE"]},{"referenceNumber":3,"citation":{"id":"16936731","citationType":"journal article","authors":["Huan C.","Kelly M.L.","Steele R.","Shapira I.","Gottesman S.R.S.","Roman C.A.J."],"citationCrossReferences":[{"database":"PubMed","id":"16936731"},{"database":"DOI","id":"10.1038/ni1378"}],"title":"Transcription factors TFE3 and TFEB are critical for CD40 ligand expression and thymus-dependent humoral immunity.","publicationDate":"2006","journal":"Nat. Immunol.","firstPage":"1082","lastPage":"1091","volume":"7"},"referencePositions":["FUNCTION","DNA-BINDING","TISSUE SPECIFICITY"]},{"referenceNumber":4,"citation":{"id":"17242355","citationType":"journal article","authors":["Villen J.","Beausoleil S.A.","Gerber S.A.","Gygi S.P."],"citationCrossReferences":[{"database":"PubMed","id":"17242355"},{"database":"DOI","id":"10.1073/pnas.0609836104"}],"title":"Large-scale phosphorylation analysis of mouse liver.","publicationDate":"2007","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"1488","lastPage":"1493","volume":"104"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"Liver","type":"TISSUE"}]},{"referenceNumber":5,"citation":{"id":"19144319","citationType":"journal article","authors":["Trost M.","English L.","Lemieux S.","Courcelles M.","Desjardins M.","Thibault P."],"citationCrossReferences":[{"database":"PubMed","id":"19144319"},{"database":"DOI","id":"10.1016/j.immuni.2008.11.006"}],"title":"The phagosomal proteome in interferon-gamma-activated macrophages.","publicationDate":"2009","journal":"Immunity","firstPage":"143","lastPage":"154","volume":"30"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]},{"referenceNumber":6,"citation":{"id":"21183079","citationType":"journal article","authors":["Huttlin E.L.","Jedrychowski M.P.","Elias J.E.","Goswami T.","Rad R.","Beausoleil S.A.","Villen J.","Haas W.","Sowa M.E.","Gygi S.P."],"citationCrossReferences":[{"database":"PubMed","id":"21183079"},{"database":"DOI","id":"10.1016/j.cell.2010.12.001"}],"title":"A tissue-specific atlas of mouse protein phosphorylation and expression.","publicationDate":"2010","journal":"Cell","firstPage":"1174","lastPage":"1189","volume":"143"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-108; SER-113; SER-121; SER-137; SER-141; SER-465; SER-466 AND SER-468","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"Brain","type":"TISSUE"},{"value":"Brown adipose tissue","type":"TISSUE"},{"value":"Heart","type":"TISSUE"},{"value":"Kidney","type":"TISSUE"},{"value":"Lung","type":"TISSUE"},{"value":"Spleen","type":"TISSUE"},{"value":"Testis","type":"TISSUE"}]},{"referenceNumber":7,"citation":{"id":"22343943","citationType":"journal article","authors":["Settembre C.","Zoncu R.","Medina D.L.","Vetrini F.","Erdin S.","Erdin S.","Huynh T.","Ferron M.","Karsenty G.","Vellard M.C.","Facchinetti V.","Sabatini D.M.","Ballabio A."],"citationCrossReferences":[{"database":"PubMed","id":"22343943"},{"database":"DOI","id":"10.1038/emboj.2012.32"}],"title":"A lysosome-to-nucleus signalling mechanism senses and regulates the lysosome via mTOR and TFEB.","publicationDate":"2012","journal":"EMBO J.","firstPage":"1095","lastPage":"1108","volume":"31"},"referencePositions":["FUNCTION"]},{"referenceNumber":8,"citation":{"id":"24882217","citationType":"journal article","authors":["Visvikis O.","Ihuegbu N.","Labed S.A.","Luhachack L.G.","Alves A.M.","Wollenberg A.C.","Stuart L.M.","Stormo G.D.","Irazoqui J.E."],"citationCrossReferences":[{"database":"PubMed","id":"24882217"},{"database":"DOI","id":"10.1016/j.immuni.2014.05.002"}],"title":"Innate host defense requires TFEB-mediated transcription of cytoprotective and antimicrobial genes.","publicationDate":"2014","journal":"Immunity","firstPage":"896","lastPage":"909","volume":"40"},"referencePositions":["FUNCTION"],"evidences":[{"evidenceCode":"ECO:0000305"}]},{"referenceNumber":9,"citation":{"id":"27184844","citationType":"journal article","authors":["Najibi M.","Labed S.A.","Visvikis O.","Irazoqui J.E."],"citationCrossReferences":[{"database":"PubMed","id":"27184844"},{"database":"DOI","id":"10.1016/j.celrep.2016.04.052"}],"title":"An evolutionarily conserved PLC-PKD-TFEB pathway for host defense.","publicationDate":"2016","journal":"Cell Rep.","firstPage":"1728","lastPage":"1742","volume":"15"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION"]},{"referenceNumber":10,"citation":{"id":"27278822","citationType":"journal article","authors":["Bento C.F.","Ashkenazi A.","Jimenez-Sanchez M.","Rubinsztein D.C."],"citationCrossReferences":[{"database":"PubMed","id":"27278822"},{"database":"DOI","id":"10.1038/ncomms11803"}],"title":"The Parkinson's disease-associated genes ATP13A2 and SYT11 regulate autophagy via a common pathway.","publicationDate":"2016","journal":"Nat. Commun.","firstPage":"11803","lastPage":"11803","volume":"7"},"referencePositions":["SUBCELLULAR LOCATION","FUNCTION"]},{"referenceNumber":11,"citation":{"id":"29146937","citationType":"journal article","authors":["Willett R.","Martina J.A.","Zewe J.P.","Wills R.","Hammond G.R.V.","Puertollano R."],"citationCrossReferences":[{"database":"PubMed","id":"29146937"},{"database":"DOI","id":"10.1038/s41467-017-01871-z"}],"title":"TFEB regulates lysosomal positioning by modulating TMEM55B expression and JIP4 recruitment to lysosomes.","publicationDate":"2017","journal":"Nat. Commun.","firstPage":"1580","lastPage":"1580","volume":"8"},"referencePositions":["FUNCTION"]},{"referenceNumber":12,"citation":{"id":"32612235","citationType":"journal article","authors":["Napolitano G.","Di Malta C.","Esposito A.","de Araujo M.E.G.","Pece S.","Bertalot G.","Matarese M.","Benedetti V.","Zampelli A.","Stasyk T.","Siciliano D.","Venuta A.","Cesana M.","Vilardo C.","Nusco E.","Monfregola J.","Calcagni A.","Di Fiore P.P.","Huber L.A.","Ballabio A."],"citationCrossReferences":[{"database":"PubMed","id":"32612235"},{"database":"DOI","id":"10.1038/s41586-020-2444-0"}],"title":"A substrate-specific mTORC1 pathway underlies Birt-Hogg-Dube syndrome.","publicationDate":"2020","journal":"Nature","firstPage":"597","lastPage":"602","volume":"585"},"referencePositions":["SUBCELLULAR LOCATION","DISRUPTION PHENOTYPE"]},{"referenceNumber":13,"citation":{"id":"35662396","citationType":"journal article","authors":["Zhang Z.","Chen C.","Yang F.","Zeng Y.X.","Sun P.","Liu P.","Li X."],"citationCrossReferences":[{"database":"PubMed","id":"35662396"},{"database":"DOI","id":"10.1016/j.molcel.2022.05.009"}],"title":"Itaconate is a lysosomal inducer that promotes antibacterial innate immunity.","publicationDate":"2022","journal":"Mol. Cell","firstPage":"0","lastPage":"0","volume":"0"},"referencePositions":["FUNCTION","ALKYLATION AT CYS-211","MUTAGENESIS OF CYS-211"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF079095","properties":[{"key":"ProteinId","value":"AAD20979.1"},{"key":"Status","value":"ALT_INIT"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"CCDS","id":"CCDS28856.1","properties":[{"key":"Description","value":"-"}]},{"database":"CCDS","id":"CCDS50133.1","properties":[{"key":"Description","value":"-"}]},{"database":"RefSeq","id":"NP_001155194.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001161722.2"}]},{"database":"RefSeq","id":"NP_001155195.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001161723.2"}]},{"database":"RefSeq","id":"NP_001419107.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001432178.1"}]},{"database":"RefSeq","id":"NP_001419108.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001432179.1"}]},{"database":"RefSeq","id":"NP_001419109.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001432180.1"}]},{"database":"RefSeq","id":"NP_001419110.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001432181.1"}]},{"database":"RefSeq","id":"NP_035679.4","properties":[{"key":"NucleotideSequenceId","value":"NM_011549.4"}]},{"database":"RefSeq","id":"XP_006524066.2","properties":[{"key":"NucleotideSequenceId","value":"XM_006524003.4"}]},{"database":"AlphaFoldDB","id":"Q9R210","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9R210","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"204016","properties":[{"key":"Interactions","value":"16"}]},{"database":"FunCoup","id":"Q9R210","properties":[{"key":"Number of interactors","value":"787"}]},{"database":"IntAct","id":"Q9R210","properties":[{"key":"Interactions","value":"1"}]},{"database":"STRING","id":"10090.ENSMUSP00000024786","properties":[{"key":"Description","value":"-"}]},{"database":"GlyGen","id":"Q9R210","properties":[{"key":"glycosylation","value":"1 site"}]},{"database":"iPTMnet","id":"Q9R210","properties":[{"key":"Description","value":"-"}]},{"database":"PhosphoSitePlus","id":"Q9R210","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"Q9R210","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"10090-ENSMUSP00000024786","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"258862","properties":[{"key":"Description","value":"-"}]},{"database":"Pumba","id":"Q9R210","properties":[{"key":"Description","value":"-"}]},{"database":"Antibodypedia","id":"15889","properties":[{"key":"antibodies","value":"636 antibodies from 46 providers"}]},{"database":"DNASU","id":"21425","properties":[{"key":"Description","value":"-"}]},{"database":"Ensembl","id":"ENSMUST00000086932.10","properties":[{"key":"ProteinId","value":"ENSMUSP00000084151.4"},{"key":"GeneId","value":"ENSMUSG00000023990.19"}]},{"database":"Ensembl","id":"ENSMUST00000113288.8","properties":[{"key":"ProteinId","value":"ENSMUSP00000108913.2"},{"key":"GeneId","value":"ENSMUSG00000023990.19"}]},{"database":"GeneID","id":"21425","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"mmu:21425","properties":[{"key":"Description","value":"-"}]},{"database":"UCSC","id":"uc008cwc.2","properties":[{"key":"OrganismName","value":"mouse"}]},{"database":"AGR","id":"MGI:103270","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"7942","properties":[{"key":"Description","value":"-"}]},{"database":"MGI","id":"MGI:103270","properties":[{"key":"GeneName","value":"Tfeb"}]},{"database":"VEuPathDB","id":"HostDB:ENSMUSG00000023990","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG1318","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"GeneTree","id":"ENSGT00940000159691","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_031638_3_1_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q9R210","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"6242697at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q9R210","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID-ORCS","id":"21425","properties":[{"key":"hits","value":"6 hits in 79 CRISPR screens"}]},{"database":"PRO","id":"PR:Q9R210","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000589","properties":[{"key":"Component","value":"Chromosome 17"}]},{"database":"RNAct","id":"Q9R210","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"Bgee","id":"ENSMUSG00000023990","properties":[{"key":"ExpressionPatterns","value":"Expressed in hindlimb stylopod muscle and 188 other cell types or tissues"}]},{"database":"ExpressionAtlas","id":"Q9R210","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"27184844"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"27278822"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0005765","properties":[{"key":"GoTerm","value":"C:lysosomal membrane"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0005654","properties":[{"key":"GoTerm","value":"C:nucleoplasm"},{"key":"GoEvidenceType","value":"TAS:Reactome"}]},{"database":"GO","id":"GO:0005634","properties":[{"key":"GoTerm","value":"C:nucleus"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"27184844"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"27278822"}]},{"database":"GO","id":"GO:0005667","properties":[{"key":"GoTerm","value":"C:transcription regulator complex"},{"key":"GoEvidenceType","value":"IDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"15994295"}]},{"database":"GO","id":"GO:0003677","properties":[{"key":"GoTerm","value":"F:DNA binding"},{"key":"GoEvidenceType","value":"IDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"15994295"}]},{"database":"GO","id":"GO:0001228","properties":[{"key":"GoTerm","value":"F:DNA-binding transcription activator activity, RNA polymerase II-specific"},{"key":"GoEvidenceType","value":"IDA:NTNU_SB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16936731"}]},{"database":"GO","id":"GO:0003700","properties":[{"key":"GoTerm","value":"F:DNA-binding transcription factor activity"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16936731"}]},{"database":"GO","id":"GO:0000981","properties":[{"key":"GoTerm","value":"F:DNA-binding transcription factor activity, RNA polymerase II-specific"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0019899","properties":[{"key":"GoTerm","value":"F:enzyme binding"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0046982","properties":[{"key":"GoTerm","value":"F:protein heterodimerization activity"},{"key":"GoEvidenceType","value":"IPI:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000353","source":"PubMed","id":"16936731"}]},{"database":"GO","id":"GO:0000978","properties":[{"key":"GoTerm","value":"F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"},{"key":"GoEvidenceType","value":"IDA:NTNU_SB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16936731"}]},{"database":"GO","id":"GO:1990837","properties":[{"key":"GoTerm","value":"F:sequence-specific double-stranded DNA binding"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0000976","properties":[{"key":"GoTerm","value":"F:transcription cis-regulatory region binding"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16936731"}]},{"database":"GO","id":"GO:0140367","properties":[{"key":"GoTerm","value":"P:antibacterial innate immune response"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0034198","properties":[{"key":"GoTerm","value":"P:cellular response to amino acid starvation"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0009267","properties":[{"key":"GoTerm","value":"P:cellular response to starvation"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0050829","properties":[{"key":"GoTerm","value":"P:defense response to Gram-negative bacterium"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"27184844"}]},{"database":"GO","id":"GO:0001892","properties":[{"key":"GoTerm","value":"P:embryonic placenta development"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"9806910"}]},{"database":"GO","id":"GO:0006959","properties":[{"key":"GoTerm","value":"P:humoral immune response"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"16936731"}]},{"database":"GO","id":"GO:0032418","properties":[{"key":"GoTerm","value":"P:lysosome localization"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0007040","properties":[{"key":"GoTerm","value":"P:lysosome organization"},{"key":"GoEvidenceType","value":"IMP:MGI"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"31637311"}]},{"database":"GO","id":"GO:0010508","properties":[{"key":"GoTerm","value":"P:positive regulation of autophagy"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0045893","properties":[{"key":"GoTerm","value":"P:positive regulation of DNA-templated transcription"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"29146937"}]},{"database":"GO","id":"GO:0045944","properties":[{"key":"GoTerm","value":"P:positive regulation of transcription by RNA polymerase II"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16936731"}]},{"database":"GO","id":"GO:1905671","properties":[{"key":"GoTerm","value":"P:regulation of lysosome organization"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0006357","properties":[{"key":"GoTerm","value":"P:regulation of transcription by RNA polymerase II"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"CDD","id":"cd18927","properties":[{"key":"EntryName","value":"bHLHzip_TFEB"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"4.10.280.10:FF:000003","properties":[{"key":"EntryName","value":"microphthalmia-associated transcription factor isoform X1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"4.10.280.10","properties":[{"key":"EntryName","value":"Helix-loop-helix DNA-binding domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR011598","properties":[{"key":"EntryName","value":"bHLH_dom"}]},{"database":"InterPro","id":"IPR024098","properties":[{"key":"EntryName","value":"bHLHzip_TFEB"}]},{"database":"InterPro","id":"IPR036638","properties":[{"key":"EntryName","value":"HLH_DNA-bd_sf"}]},{"database":"InterPro","id":"IPR021802","properties":[{"key":"EntryName","value":"MiT/TFE_C"}]},{"database":"InterPro","id":"IPR031867","properties":[{"key":"EntryName","value":"MiT/TFE_N"}]},{"database":"PANTHER","id":"PTHR45776","properties":[{"key":"EntryName","value":"MIP04163P"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR45776:SF5","properties":[{"key":"EntryName","value":"TRANSCRIPTION FACTOR EB"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF11851","properties":[{"key":"EntryName","value":"DUF3371"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00010","properties":[{"key":"EntryName","value":"HLH"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF15951","properties":[{"key":"EntryName","value":"MITF_TFEB_C_3_N"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00353","properties":[{"key":"EntryName","value":"HLH"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF47459","properties":[{"key":"EntryName","value":"HLH, helix-loop-helix DNA-binding domain"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50888","properties":[{"key":"EntryName","value":"BHLH"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MASRIGLRMQLMREQAQQEEQRERMQQQAVMHYMQQQQQQQQQLGGPPTPAINTPVHFQSPPPVPGEVLKVQSYLENPTSYHLQQSQHQKVREYLSETYGNKFAAHVSPAQGSPKPAPAASPGVRAGHVLSTSAGNSAPNSPMAMLHISSNPEKEFDDVIDNIMRLDSVLGYINPEMQMPNTLPLSSSHLNVYSGDPQVTASMVGVTSSSCPADLTQKRELTDAESRALAKERQKKDNHNLIERRRRFNINDRIKELGMLIPKANDLDVRWNKGTILKASVDYIRRMQKDLQKSRELENHSRRLEMTNKQLWLRIQELEMQARVHGLPTTSPSGVNMAELAQQVVKQELPSEDGPGEALMLGPEVPEPEQMPALPPQAPLPSAAQPQSPFHHLDFSHGLSFGGGGDEGPTGYPDTLGTEHGSPFPNLSKKDLDLMLLDDSLLPLASDPLFSTMSPEASKASSRRSSFSMEEGDVL","length":475,"molWeight":52614,"crc64":"4249C6F501968326","md5":"E8C21950EB226749386289019BE4518C"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"DEVELOPMENTAL STAGE":1,"DOMAIN":1,"PTM":3,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1,"SEQUENCE CAUTION":1},"countByFeatureType":{"Chain":1,"Domain":1,"Region":6,"Motif":2,"Compositional bias":3,"Modified residue":14,"Mutagenesis":1},"uniParcId":"UPI00000294E8"}}