{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9QXT0","secondaryAccessions":["Q3UBH8"],"uniProtkbId":"CNPY2_MOUSE","entryAudit":{"firstPublicDate":"2004-07-19","lastAnnotationUpdateDate":"2026-06-10","lastSequenceUpdateDate":"2000-05-01","entryVersion":153,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Mus musculus","commonName":"Mouse","taxonId":10090,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Glires","Rodentia","Myomorpha","Muroidea","Muridae","Murinae","Mus","Mus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Protein canopy homolog 2"}},"alternativeNames":[{"fullName":{"value":"MIR-interacting saposin-like protein"}},{"fullName":{"value":"Putative secreted protein ZSIG9"}},{"fullName":{"value":"Transmembrane protein 4"}}],"flag":"Precursor"},"genes":[{"geneName":{"value":"Cnpy2"},"synonyms":[{"value":"Msap"},{"value":"Tmem4"},{"value":"Zsig9"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Positive regulator of neurite outgrowth by stabilizing myosin regulatory light chain (MRLC). It prevents MIR-mediated MRLC ubiquitination and its subsequent proteasomal degradation (By similarity)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Interacts with MYLIP/MIR"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"Q9QXT0","intActId":"EBI-8321111"},"interactantTwo":{"uniProtKBAccession":"Q9Z2B5","geneName":"Eif2ak3","intActId":"EBI-1226344"},"numberOfExperiments":7,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q9QXT0","intActId":"EBI-8321111"},"interactantTwo":{"uniProtKBAccession":"P11021","geneName":"HSPA5","intActId":"EBI-354921"},"numberOfExperiments":2,"organismDiffer":true}]},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU10138"}],"value":"Endoplasmic reticulum","id":"SL-0095"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the canopy family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":20,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Chain","location":{"start":{"value":21,"modifier":"EXACT"},"end":{"value":182,"modifier":"EXACT"}},"description":"Protein canopy homolog 2","featureId":"PRO_0000031667"},{"type":"Domain","location":{"start":{"value":24,"modifier":"EXACT"},"end":{"value":175,"modifier":"EXACT"}},"description":"Saposin B-type","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00415"}]},{"type":"Motif","location":{"start":{"value":179,"modifier":"EXACT"},"end":{"value":182,"modifier":"EXACT"}},"description":"Prevents secretion from ER","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU10138"}]},{"type":"Modified residue","location":{"start":{"value":115,"modifier":"EXACT"},"end":{"value":115,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"17242355"}]},{"type":"Disulfide bond","location":{"start":{"value":28,"modifier":"EXACT"},"end":{"value":171,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00415"}]},{"type":"Disulfide bond","location":{"start":{"value":31,"modifier":"EXACT"},"end":{"value":164,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00415"}]},{"type":"Disulfide bond","location":{"start":{"value":86,"modifier":"EXACT"},"end":{"value":137,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00415"}]}],"keywords":[{"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"id":"KW-0256","category":"Cellular component","name":"Endoplasmic reticulum"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0732","category":"Domain","name":"Signal"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-TC9P1QHMJ360","citationType":"submission","authors":["Sheppard P.","Jelinek L.","Whitmore T.","Blumberg H.","Lehner J.","O'Hara P."],"title":"Mus musculus putative secreted protein.","publicationDate":"SEP-1999","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]"]},{"referenceNumber":2,"citation":{"id":"16141072","citationType":"journal article","authors":["Carninci P.","Kasukawa T.","Katayama S.","Gough J.","Frith M.C.","Maeda N.","Oyama R.","Ravasi T.","Lenhard B.","Wells C.","Kodzius R.","Shimokawa K.","Bajic V.B.","Brenner S.E.","Batalov S.","Forrest A.R.","Zavolan M.","Davis M.J.","Wilming L.G.","Aidinis V.","Allen J.E.","Ambesi-Impiombato A.","Apweiler R.","Aturaliya R.N.","Bailey T.L.","Bansal M.","Baxter L.","Beisel K.W.","Bersano T.","Bono H.","Chalk A.M.","Chiu K.P.","Choudhary V.","Christoffels A.","Clutterbuck D.R.","Crowe M.L.","Dalla E.","Dalrymple B.P.","de Bono B.","Della Gatta G.","di Bernardo D.","Down T.","Engstrom P.","Fagiolini M.","Faulkner G.","Fletcher C.F.","Fukushima T.","Furuno M.","Futaki S.","Gariboldi M.","Georgii-Hemming P.","Gingeras T.R.","Gojobori T.","Green R.E.","Gustincich S.","Harbers M.","Hayashi Y.","Hensch T.K.","Hirokawa N.","Hill D.","Huminiecki L.","Iacono M.","Ikeo K.","Iwama A.","Ishikawa T.","Jakt M.","Kanapin A.","Katoh M.","Kawasawa Y.","Kelso J.","Kitamura H.","Kitano H.","Kollias G.","Krishnan S.P.","Kruger A.","Kummerfeld S.K.","Kurochkin I.V.","Lareau L.F.","Lazarevic D.","Lipovich L.","Liu J.","Liuni S.","McWilliam S.","Madan Babu M.","Madera M.","Marchionni L.","Matsuda H.","Matsuzawa S.","Miki H.","Mignone F.","Miyake S.","Morris K.","Mottagui-Tabar S.","Mulder N.","Nakano N.","Nakauchi H.","Ng P.","Nilsson R.","Nishiguchi S.","Nishikawa S.","Nori F.","Ohara O.","Okazaki Y.","Orlando V.","Pang K.C.","Pavan W.J.","Pavesi G.","Pesole G.","Petrovsky N.","Piazza S.","Reed J.","Reid J.F.","Ring B.Z.","Ringwald M.","Rost B.","Ruan Y.","Salzberg S.L.","Sandelin A.","Schneider C.","Schoenbach C.","Sekiguchi K.","Semple C.A.","Seno S.","Sessa L.","Sheng Y.","Shibata Y.","Shimada H.","Shimada K.","Silva D.","Sinclair B.","Sperling S.","Stupka E.","Sugiura K.","Sultana R.","Takenaka Y.","Taki K.","Tammoja K.","Tan S.L.","Tang S.","Taylor M.S.","Tegner J.","Teichmann S.A.","Ueda H.R.","van Nimwegen E.","Verardo R.","Wei C.L.","Yagi K.","Yamanishi H.","Zabarovsky E.","Zhu S.","Zimmer A.","Hide W.","Bult C.","Grimmond S.M.","Teasdale R.D.","Liu E.T.","Brusic V.","Quackenbush J.","Wahlestedt C.","Mattick J.S.","Hume D.A.","Kai C.","Sasaki D.","Tomaru Y.","Fukuda S.","Kanamori-Katayama M.","Suzuki M.","Aoki J.","Arakawa T.","Iida J.","Imamura K.","Itoh M.","Kato T.","Kawaji H.","Kawagashira N.","Kawashima T.","Kojima M.","Kondo S.","Konno H.","Nakano K.","Ninomiya N.","Nishio T.","Okada M.","Plessy C.","Shibata K.","Shiraki T.","Suzuki S.","Tagami M.","Waki K.","Watahiki A.","Okamura-Oho Y.","Suzuki H.","Kawai J.","Hayashizaki Y."],"citationCrossReferences":[{"database":"PubMed","id":"16141072"},{"database":"DOI","id":"10.1126/science.1112014"}],"title":"The transcriptional landscape of the mammalian genome.","publicationDate":"2005","journal":"Science","firstPage":"1559","lastPage":"1563","volume":"309"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"C57BL/6J","type":"STRAIN"},{"value":"Bone marrow","type":"TISSUE"},{"value":"Hippocampus","type":"TISSUE"},{"value":"Pancreas","type":"TISSUE"},{"value":"Pituitary","type":"TISSUE"}]},{"referenceNumber":3,"citation":{"id":"15489334","citationType":"journal article","authoringGroup":["The MGC Project Team"],"citationCrossReferences":[{"database":"PubMed","id":"15489334"},{"database":"DOI","id":"10.1101/gr.2596504"}],"title":"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).","publicationDate":"2004","journal":"Genome Res.","firstPage":"2121","lastPage":"2127","volume":"14"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"129","type":"STRAIN"},{"value":"Mammary tumor","type":"TISSUE"}]},{"referenceNumber":4,"citation":{"id":"17242355","citationType":"journal article","authors":["Villen J.","Beausoleil S.A.","Gerber S.A.","Gygi S.P."],"citationCrossReferences":[{"database":"PubMed","id":"17242355"},{"database":"DOI","id":"10.1073/pnas.0609836104"}],"title":"Large-scale phosphorylation analysis of mouse liver.","publicationDate":"2007","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"1488","lastPage":"1493","volume":"104"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-115","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"Liver","type":"TISSUE"}]},{"referenceNumber":5,"citation":{"id":"21183079","citationType":"journal article","authors":["Huttlin E.L.","Jedrychowski M.P.","Elias J.E.","Goswami T.","Rad R.","Beausoleil S.A.","Villen J.","Haas W.","Sowa M.E.","Gygi S.P."],"citationCrossReferences":[{"database":"PubMed","id":"21183079"},{"database":"DOI","id":"10.1016/j.cell.2010.12.001"}],"title":"A tissue-specific atlas of mouse protein phosphorylation and expression.","publicationDate":"2010","journal":"Cell","firstPage":"1174","lastPage":"1189","volume":"143"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"Brain","type":"TISSUE"},{"value":"Brown adipose tissue","type":"TISSUE"},{"value":"Heart","type":"TISSUE"},{"value":"Kidney","type":"TISSUE"},{"value":"Liver","type":"TISSUE"},{"value":"Lung","type":"TISSUE"},{"value":"Pancreas","type":"TISSUE"},{"value":"Spleen","type":"TISSUE"},{"value":"Testis","type":"TISSUE"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF186115","properties":[{"key":"ProteinId","value":"AAF01433.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK007914","properties":[{"key":"ProteinId","value":"BAB25346.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK013014","properties":[{"key":"ProteinId","value":"BAB28597.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK013568","properties":[{"key":"ProteinId","value":"BAB28909.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK019927","properties":[{"key":"ProteinId","value":"BAB31921.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK150953","properties":[{"key":"ProteinId","value":"BAE29986.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC008261","properties":[{"key":"ProteinId","value":"AAH08261.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"CCDS","id":"CCDS24272.1","properties":[{"key":"Description","value":"-"}]},{"database":"RefSeq","id":"NP_001416158.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001429229.1"}]},{"database":"RefSeq","id":"NP_001416159.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001429230.1"}]},{"database":"RefSeq","id":"NP_001416160.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001429231.1"}]},{"database":"RefSeq","id":"NP_001416161.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001429232.1"}]},{"database":"RefSeq","id":"NP_064337.1","properties":[{"key":"NucleotideSequenceId","value":"NM_019953.2"}]},{"database":"AlphaFoldDB","id":"Q9QXT0","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9QXT0","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"208040","properties":[{"key":"Interactions","value":"4"}]},{"database":"FunCoup","id":"Q9QXT0","properties":[{"key":"Number of interactors","value":"2198"}]},{"database":"IntAct","id":"Q9QXT0","properties":[{"key":"Interactions","value":"4"}]},{"database":"MINT","id":"Q9QXT0","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"10090.ENSMUSP00000151918","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"Q9QXT0","properties":[{"key":"Description","value":"-"}]},{"database":"PhosphoSitePlus","id":"Q9QXT0","properties":[{"key":"Description","value":"-"}]},{"database":"SwissPalm","id":"Q9QXT0","properties":[{"key":"Description","value":"-"}]},{"database":"REPRODUCTION-2DPAGE","id":"IPI00135512","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"Q9QXT0","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"10090-ENSMUSP00000151918","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"Q9QXT0","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"283655","properties":[{"key":"Description","value":"-"}]},{"database":"Pumba","id":"Q9QXT0","properties":[{"key":"Description","value":"-"}]},{"database":"TopDownProteomics","id":"Q9QXT0","properties":[{"key":"Description","value":"-"}]},{"database":"DNASU","id":"56530","properties":[{"key":"Description","value":"-"}]},{"database":"Ensembl","id":"ENSMUST00000026446.4","properties":[{"key":"ProteinId","value":"ENSMUSP00000026446.3"},{"key":"GeneId","value":"ENSMUSG00000025381.4"}]},{"database":"Ensembl","id":"ENSMUST00000219037.2","properties":[{"key":"ProteinId","value":"ENSMUSP00000151918.2"},{"key":"GeneId","value":"ENSMUSG00000025381.4"}]},{"database":"GeneID","id":"56530","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"mmu:56530","properties":[{"key":"Description","value":"-"}]},{"database":"UCSC","id":"uc007hmi.1","properties":[{"key":"OrganismName","value":"mouse"}]},{"database":"AGR","id":"MGI:1928477","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"10330","properties":[{"key":"Description","value":"-"}]},{"database":"MGI","id":"MGI:1928477","properties":[{"key":"GeneName","value":"Cnpy2"}]},{"database":"VEuPathDB","id":"HostDB:ENSMUSG00000025381","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG3782","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"GeneTree","id":"ENSGT00940000161158","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_095726_2_1_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q9QXT0","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"HLKCLVC","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"192915at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q9QXT0","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID-ORCS","id":"56530","properties":[{"key":"hits","value":"1 hit in 78 CRISPR screens"}]},{"database":"PRO","id":"PR:Q9QXT0","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000589","properties":[{"key":"Component","value":"Chromosome 10"}]},{"database":"RNAct","id":"Q9QXT0","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"Bgee","id":"ENSMUSG00000025381","properties":[{"key":"ExpressionPatterns","value":"Expressed in renal medulla collecting duct and 260 other cell types or tissues"}]},{"database":"ExpressionAtlas","id":"Q9QXT0","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0005783","properties":[{"key":"GoTerm","value":"C:endoplasmic reticulum"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0010629","properties":[{"key":"GoTerm","value":"P:negative regulation of gene expression"},{"key":"GoEvidenceType","value":"IMP:BHF-UCL"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"22378787"}]},{"database":"GO","id":"GO:0010988","properties":[{"key":"GoTerm","value":"P:regulation of low-density lipoprotein particle clearance"},{"key":"GoEvidenceType","value":"IMP:BHF-UCL"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"22378787"}]},{"database":"Gene3D","id":"1.10.225.10","properties":[{"key":"EntryName","value":"Saposin-like"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR042415","properties":[{"key":"EntryName","value":"CNPY"}]},{"database":"InterPro","id":"IPR021852","properties":[{"key":"EntryName","value":"DUF3456"}]},{"database":"InterPro","id":"IPR008139","properties":[{"key":"EntryName","value":"SaposinB_dom"}]},{"database":"PANTHER","id":"PTHR13341","properties":[{"key":"EntryName","value":"MIR-INTERACTING SAPOSIN-LIKE PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR13341:SF6","properties":[{"key":"EntryName","value":"PROTEIN CANOPY HOMOLOG 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF11938","properties":[{"key":"EntryName","value":"DUF3456"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00014","properties":[{"key":"EntryName","value":"ER_TARGET"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50015","properties":[{"key":"EntryName","value":"SAP_B"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKGWGWLALLLGVLLGTAWARRSQDLHCGACRALVDELEWEIARVDPKKTIQMGSFRINPDGSQSVVEVPYARSEAHLTELLEEVCDRMKEYGEQIDPSTHRKNYVRVVSRNGESSELDLQGIRIDSDISGTLKFACESIVEEYEDELIEFFSREADNVKDKLCSKRTDLCDHALHRSHDEL","length":182,"molWeight":20767,"crc64":"83E54E7F01EE9B87","md5":"2AB6AC29468F5FA1490A087EA35E8B73"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"INTERACTION":2,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Signal":1,"Chain":1,"Domain":1,"Motif":1,"Modified residue":1,"Disulfide bond":3},"uniParcId":"UPI00000225F3"}}