{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9NXW2","secondaryAccessions":["B7Z7I3","Q9H6H0"],"uniProtkbId":"DJB12_HUMAN","entryAudit":{"firstPublicDate":"2001-10-18","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2018-10-10","entryVersion":201,"sequenceVersion":5},"annotationScore":5.0,"organism":{"scientificName":"Homo sapiens","commonName":"Human","taxonId":9606,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Primates","Haplorrhini","Catarrhini","Hominidae","Homo"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"21150129"}],"value":"DnaJ homolog subfamily B member 12"}}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"21150129"},{"evidenceCode":"ECO:0000312","source":"HGNC","id":"HGNC:14891"}],"value":"DNAJB12"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21148293"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21150129"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24732912"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27916661"}],"value":"Acts as a co-chaperone with HSPA8/Hsc70; required to promote protein folding and trafficking, prevent aggregation of client proteins, and promote unfolded proteins to endoplasmic reticulum-associated degradation (ERAD) pathway (PubMed:21148293, PubMed:21150129). Acts by determining HSPA8/Hsc70's ATPase and polypeptide-binding activities (PubMed:21148293). Can also act independently of HSPA8/Hsc70: together with DNAJB14, acts as a chaperone that promotes maturation of potassium channels KCND2 and KCNH2 by stabilizing nascent channel subunits and assembling them into tetramers (PubMed:27916661). While stabilization of nascent channel proteins is dependent on HSPA8/Hsc70, the process of oligomerization of channel subunits is independent of HSPA8/Hsc70 (PubMed:27916661). When overexpressed, forms membranous structures together with DNAJB14 and HSPA8/Hsc70 within the nucleus; the role of these structures, named DJANGOs, is still unclear (PubMed:24732912)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21673190"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24675744"}],"value":"(Microbial infection) In case of infection by polyomavirus, involved in the virus endoplasmic reticulum membrane penetration and infection (PubMed:21673190, PubMed:24675744)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21148293"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21150129"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24675744"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24732912"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27916661"}],"value":"Homodimer and homotetramer (PubMed:27916661). Interacts (via J domain) with HSPA8/Hsc70 (PubMed:21148293, PubMed:21150129, PubMed:24732912, PubMed:27916661). Forms a multiprotein complex, at least composed of DNAJB12, DNAJB14, HSPA8/Hsc70 and SGTA; interaction with DNAJB14 and HSPA8/Hsc70 is direct (PubMed:24675744)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21148293"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21150129"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24732912"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27916661"}],"value":"Localizes to the endoplasmic reticulum membrane (PubMed:21148293, PubMed:21150129, PubMed:24732912, PubMed:27916661). When overexpressed, forms membranous structures in the nucleus (PubMed:24732912)"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21148293"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21150129"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24732912"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27916661"}],"value":"Endoplasmic reticulum membrane","id":"SL-0097"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255"}],"value":"Single-pass membrane protein","id":"SL-9904"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24732912"}],"value":"Nucleus membrane","id":"SL-0182"},"topology":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Single-pass membrane protein","id":"SL-9904"}}]},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25631089"}],"value":"(Microbial infection) Upon SV40 infection, colocalizes with BCAP31, DNAJC18 and DNAJB14 in punctate structures within the endoplasmic reticulum membrane"}]}},{"commentType":"ALTERNATIVE PRODUCTS","events":["Alternative splicing"],"isoforms":[{"name":{"value":"1"},"isoformIds":["Q9NXW2-1"],"isoformSequenceStatus":"Displayed"},{"name":{"value":"2"},"isoformIds":["Q9NXW2-2"],"sequenceIds":["VSP_039040"],"isoformSequenceStatus":"Described"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"33563959"}],"value":"Methylated at His-185 by METTL9"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the DnaJ family. DNAJB12/DNAJB14 subfamily"}],"commentType":"SIMILARITY"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"BAB15289.1","note":"Truncated N-terminus.","evidences":[{"evidenceCode":"ECO:0000305"}]},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"BAH13619.1","note":"Truncated N-terminus.","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":375,"modifier":"EXACT"}},"description":"DnaJ homolog subfamily B member 12","featureId":"PRO_0000071037"},{"type":"Topological domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":243,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"21148293"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"21150129"}]},{"type":"Transmembrane","location":{"start":{"value":244,"modifier":"EXACT"},"end":{"value":264,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":265,"modifier":"EXACT"},"end":{"value":375,"modifier":"EXACT"}},"description":"Lumenal","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"21148293"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"21150129"}]},{"type":"Domain","location":{"start":{"value":112,"modifier":"EXACT"},"end":{"value":176,"modifier":"EXACT"}},"description":"J","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00286"}]},{"type":"Region","location":{"start":{"value":45,"modifier":"EXACT"},"end":{"value":92,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":49,"modifier":"EXACT"},"end":{"value":58,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Modified residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"N-acetylmethionine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"22814378"}]},{"type":"Modified residue","location":{"start":{"value":185,"modifier":"EXACT"},"end":{"value":185,"modifier":"EXACT"}},"description":"Pros-methylhistidine","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"33563959"}]},{"type":"Alternative sequence","location":{"start":{"value":369,"modifier":"EXACT"},"end":{"value":375,"modifier":"EXACT"}},"description":"in isoform 2","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"14702039"}],"featureId":"VSP_039040","alternativeSequence":{"originalSequence":"VQASLHG","alternativeSequences":["TMKSLENFW"]}},{"type":"Natural variant","location":{"start":{"value":304,"modifier":"EXACT"},"end":{"value":304,"modifier":"EXACT"}},"description":"in dbSNP:rs3750784","featureCrossReferences":[{"database":"dbSNP","id":"rs3750784"}],"featureId":"VAR_017864","alternativeSequence":{"originalSequence":"E","alternativeSequences":["K"]}},{"type":"Mutagenesis","location":{"start":{"value":138,"modifier":"EXACT"},"end":{"value":138,"modifier":"EXACT"}},"description":"Abolishes interaction with HSPA8/Hsc70.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21148293"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21150129"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24732912"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["Q"]}},{"type":"Mutagenesis","location":{"start":{"value":184,"modifier":"EXACT"},"end":{"value":212,"modifier":"EXACT"}},"description":"In delta(G/F); impaired ability to form tetramers and impaired ability to promote potassium channel assembly into tetramers.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27916661"}],"alternativeSequence":{}},{"type":"Mutagenesis","location":{"start":{"value":185,"modifier":"EXACT"},"end":{"value":185,"modifier":"EXACT"}},"description":"Abolished histidine methylation by METTL9.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33563959"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["R"]}},{"type":"Sequence conflict","location":{"start":{"value":70,"modifier":"EXACT"},"end":{"value":70,"modifier":"EXACT"}},"description":"in Ref. 1; BAA90896","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["S"]}},{"type":"Sequence conflict","location":{"start":{"value":212,"modifier":"EXACT"},"end":{"value":212,"modifier":"EXACT"}},"description":"in Ref. 1; BAA90896","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"F","alternativeSequences":["S"]}},{"type":"Sequence conflict","location":{"start":{"value":215,"modifier":"EXACT"},"end":{"value":215,"modifier":"EXACT"}},"description":"in Ref. 1; BAH13619","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["G"]}},{"type":"Helix","location":{"start":{"value":111,"modifier":"EXACT"},"end":{"value":115,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2CTP"}]},{"type":"Helix","location":{"start":{"value":123,"modifier":"EXACT"},"end":{"value":134,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2CTP"}]},{"type":"Turn","location":{"start":{"value":139,"modifier":"EXACT"},"end":{"value":141,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2CTP"}]},{"type":"Helix","location":{"start":{"value":145,"modifier":"EXACT"},"end":{"value":161,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2CTP"}]},{"type":"Helix","location":{"start":{"value":164,"modifier":"EXACT"},"end":{"value":172,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2CTP"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0007","category":"PTM","name":"Acetylation"},{"id":"KW-0025","category":"Coding sequence diversity","name":"Alternative splicing"},{"id":"KW-0143","category":"Molecular function","name":"Chaperone"},{"id":"KW-0256","category":"Cellular component","name":"Endoplasmic reticulum"},{"id":"KW-0945","category":"Biological process","name":"Host-virus interaction"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0488","category":"PTM","name":"Methylation"},{"id":"KW-0539","category":"Cellular component","name":"Nucleus"},{"id":"KW-1267","category":"Technical term","name":"Proteomics identification"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"14702039","citationType":"journal article","authors":["Ota T.","Suzuki Y.","Nishikawa T.","Otsuki T.","Sugiyama T.","Irie R.","Wakamatsu A.","Hayashi K.","Sato H.","Nagai K.","Kimura K.","Makita H.","Sekine M.","Obayashi M.","Nishi T.","Shibahara T.","Tanaka T.","Ishii S.","Yamamoto J.","Saito K.","Kawai Y.","Isono Y.","Nakamura Y.","Nagahari K.","Murakami K.","Yasuda T.","Iwayanagi T.","Wagatsuma M.","Shiratori A.","Sudo H.","Hosoiri T.","Kaku Y.","Kodaira H.","Kondo H.","Sugawara M.","Takahashi M.","Kanda K.","Yokoi T.","Furuya T.","Kikkawa E.","Omura Y.","Abe K.","Kamihara K.","Katsuta N.","Sato K.","Tanikawa M.","Yamazaki M.","Ninomiya K.","Ishibashi T.","Yamashita H.","Murakawa K.","Fujimori K.","Tanai H.","Kimata M.","Watanabe M.","Hiraoka S.","Chiba Y.","Ishida S.","Ono Y.","Takiguchi S.","Watanabe S.","Yosida M.","Hotuta T.","Kusano J.","Kanehori K.","Takahashi-Fujii A.","Hara H.","Tanase T.-O.","Nomura Y.","Togiya S.","Komai F.","Hara R.","Takeuchi K.","Arita M.","Imose N.","Musashino K.","Yuuki H.","Oshima A.","Sasaki N.","Aotsuka S.","Yoshikawa Y.","Matsunawa H.","Ichihara T.","Shiohata N.","Sano S.","Moriya S.","Momiyama H.","Satoh N.","Takami S.","Terashima Y.","Suzuki O.","Nakagawa S.","Senoh A.","Mizoguchi H.","Goto Y.","Shimizu F.","Wakebe H.","Hishigaki H.","Watanabe T.","Sugiyama A.","Takemoto M.","Kawakami B.","Yamazaki M.","Watanabe K.","Kumagai A.","Itakura S.","Fukuzumi Y.","Fujimori Y.","Komiyama M.","Tashiro H.","Tanigami A.","Fujiwara T.","Ono T.","Yamada K.","Fujii Y.","Ozaki K.","Hirao M.","Ohmori Y.","Kawabata A.","Hikiji T.","Kobatake N.","Inagaki H.","Ikema Y.","Okamoto S.","Okitani R.","Kawakami T.","Noguchi S.","Itoh T.","Shigeta K.","Senba T.","Matsumura K.","Nakajima Y.","Mizuno T.","Morinaga M.","Sasaki M.","Togashi T.","Oyama M.","Hata H.","Watanabe M.","Komatsu T.","Mizushima-Sugano J.","Satoh T.","Shirai Y.","Takahashi Y.","Nakagawa K.","Okumura K.","Nagase T.","Nomura N.","Kikuchi H.","Masuho Y.","Yamashita R.","Nakai K.","Yada T.","Nakamura Y.","Ohara O.","Isogai T.","Sugano S."],"citationCrossReferences":[{"database":"PubMed","id":"14702039"},{"database":"DOI","id":"10.1038/ng1285"}],"title":"Complete sequencing and characterization of 21,243 full-length human cDNAs.","publicationDate":"2004","journal":"Nat. Genet.","firstPage":"40","lastPage":"45","volume":"36"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2)"],"referenceComments":[{"value":"Adipose tissue","type":"TISSUE"},{"value":"Kidney epithelium","type":"TISSUE"},{"value":"Testis","type":"TISSUE"}]},{"referenceNumber":2,"citation":{"id":"15164054","citationType":"journal article","authors":["Deloukas P.","Earthrowl M.E.","Grafham D.V.","Rubenfield M.","French L.","Steward C.A.","Sims S.K.","Jones M.C.","Searle S.","Scott C.","Howe K.","Hunt S.E.","Andrews T.D.","Gilbert J.G.R.","Swarbreck D.","Ashurst J.L.","Taylor A.","Battles J.","Bird C.P.","Ainscough R.","Almeida J.P.","Ashwell R.I.S.","Ambrose K.D.","Babbage A.K.","Bagguley C.L.","Bailey J.","Banerjee R.","Bates K.","Beasley H.","Bray-Allen S.","Brown A.J.","Brown J.Y.","Burford D.C.","Burrill W.","Burton J.","Cahill P.","Camire D.","Carter N.P.","Chapman J.C.","Clark S.Y.","Clarke G.","Clee C.M.","Clegg S.","Corby N.","Coulson A.","Dhami P.","Dutta I.","Dunn M.","Faulkner L.","Frankish A.","Frankland J.A.","Garner P.","Garnett J.","Gribble S.","Griffiths C.","Grocock R.","Gustafson E.","Hammond S.","Harley J.L.","Hart E.","Heath P.D.","Ho T.P.","Hopkins B.","Horne J.","Howden P.J.","Huckle E.","Hynds C.","Johnson C.","Johnson D.","Kana A.","Kay M.","Kimberley A.M.","Kershaw J.K.","Kokkinaki M.","Laird G.K.","Lawlor S.","Lee H.M.","Leongamornlert D.A.","Laird G.","Lloyd C.","Lloyd D.M.","Loveland J.","Lovell J.","McLaren S.","McLay K.E.","McMurray A.","Mashreghi-Mohammadi M.","Matthews L.","Milne S.","Nickerson T.","Nguyen M.","Overton-Larty E.","Palmer S.A.","Pearce A.V.","Peck A.I.","Pelan S.","Phillimore B.","Porter K.","Rice C.M.","Rogosin A.","Ross M.T.","Sarafidou T.","Sehra H.K.","Shownkeen R.","Skuce C.D.","Smith M.","Standring L.","Sycamore N.","Tester J.","Thorpe A.","Torcasso W.","Tracey A.","Tromans A.","Tsolas J.","Wall M.","Walsh J.","Wang H.","Weinstock K.","West A.P.","Willey D.L.","Whitehead S.L.","Wilming L.","Wray P.W.","Young L.","Chen Y.","Lovering R.C.","Moschonas N.K.","Siebert R.","Fechtel K.","Bentley D.","Durbin R.M.","Hubbard T.","Doucette-Stamm L.","Beck S.","Smith D.R.","Rogers J."],"citationCrossReferences":[{"database":"PubMed","id":"15164054"},{"database":"DOI","id":"10.1038/nature02462"}],"title":"The DNA sequence and comparative analysis of human chromosome 10.","publicationDate":"2004","journal":"Nature","firstPage":"375","lastPage":"381","volume":"429"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"]},{"referenceNumber":3,"citation":{"id":"15489334","citationType":"journal article","authoringGroup":["The MGC Project Team"],"citationCrossReferences":[{"database":"PubMed","id":"15489334"},{"database":"DOI","id":"10.1101/gr.2596504"}],"title":"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).","publicationDate":"2004","journal":"Genome Res.","firstPage":"2121","lastPage":"2127","volume":"14"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)"],"referenceComments":[{"value":"Colon","type":"TISSUE"},{"value":"Pancreas","type":"TISSUE"}]},{"referenceNumber":4,"citation":{"id":"21150129","citationType":"journal article","authors":["Yamamoto Y.H.","Kimura T.","Momohara S.","Takeuchi M.","Tani T.","Kimata Y.","Kadokura H.","Kohno K."],"citationCrossReferences":[{"database":"PubMed","id":"21150129"},{"database":"DOI","id":"10.1247/csf.10023"}],"title":"A novel ER J-protein DNAJB12 accelerates ER-associated degradation of membrane proteins including CFTR.","publicationDate":"2010","journal":"Cell Struct. Funct.","firstPage":"107","lastPage":"116","volume":"35"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION","TOPOLOGY","INTERACTION WITH HSPA8","MUTAGENESIS OF HIS-138"]},{"referenceNumber":5,"citation":{"id":"21269460","citationType":"journal article","authors":["Burkard T.R.","Planyavsky M.","Kaupe I.","Breitwieser F.P.","Buerckstuemmer T.","Bennett K.L.","Superti-Furga G.","Colinge J."],"citationCrossReferences":[{"database":"PubMed","id":"21269460"},{"database":"DOI","id":"10.1186/1752-0509-5-17"}],"title":"Initial characterization of the human central proteome.","publicationDate":"2011","journal":"BMC Syst. Biol.","firstPage":"17","lastPage":"17","volume":"5"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]},{"referenceNumber":6,"citation":{"id":"21673190","citationType":"journal article","authors":["Goodwin E.C.","Lipovsky A.","Inoue T.","Magaldi T.G.","Edwards A.P.","Van Goor K.E.","Paton A.W.","Paton J.C.","Atwood W.J.","Tsai B.","DiMaio D."],"citationCrossReferences":[{"database":"PubMed","id":"21673190"},{"database":"DOI","id":"10.1128/mbio.00101-11"}],"title":"BiP and multiple DNAJ molecular chaperones in the endoplasmic reticulum are required for efficient simian virus 40 infection.","publicationDate":"2011","journal":"MBio","firstPage":"E00101","lastPage":"E00111","volume":"2"},"referencePositions":["FUNCTION (MICROBIAL INFECTION)"]},{"referenceNumber":7,"citation":{"id":"21148293","citationType":"journal article","authors":["Grove D.E.","Fan C.Y.","Ren H.Y.","Cyr D.M."],"citationCrossReferences":[{"database":"PubMed","id":"21148293"},{"database":"DOI","id":"10.1091/mbc.e10-09-0760"}],"title":"The endoplasmic reticulum-associated Hsp40 DNAJB12 and Hsc70 cooperate to facilitate RMA1 E3-dependent degradation of nascent CFTRDeltaF508.","publicationDate":"2011","journal":"Mol. Biol. Cell","firstPage":"301","lastPage":"314","volume":"22"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION","TOPOLOGY","INTERACTION WITH HSPA8","MUTAGENESIS OF HIS-138"]},{"referenceNumber":8,"citation":{"id":"22814378","citationType":"journal article","authors":["Van Damme P.","Lasa M.","Polevoda B.","Gazquez C.","Elosegui-Artola A.","Kim D.S.","De Juan-Pardo E.","Demeyer K.","Hole K.","Larrea E.","Timmerman E.","Prieto J.","Arnesen T.","Sherman F.","Gevaert K.","Aldabe R."],"citationCrossReferences":[{"database":"PubMed","id":"22814378"},{"database":"DOI","id":"10.1073/pnas.1210303109"}],"title":"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.","publicationDate":"2012","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"12449","lastPage":"12454","volume":"109"},"referencePositions":["ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]},{"referenceNumber":9,"citation":{"id":"24732912","citationType":"journal article","authors":["Goodwin E.C.","Motamedi N.","Lipovsky A.","Fernandez-Busnadiego R.","DiMaio D."],"citationCrossReferences":[{"database":"PubMed","id":"24732912"},{"database":"DOI","id":"10.1371/journal.pone.0094322"}],"title":"Expression of DNAJB12 or DNAJB14 causes coordinate invasion of the nucleus by membranes associated with a novel nuclear pore structure.","publicationDate":"2014","journal":"PLoS ONE","firstPage":"E94322","lastPage":"E94322","volume":"9"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION","INTERACTION WITH HSPA8","MUTAGENESIS OF HIS-138"]},{"referenceNumber":10,"citation":{"id":"24675744","citationType":"journal article","authors":["Walczak C.P.","Ravindran M.S.","Inoue T.","Tsai B."],"citationCrossReferences":[{"database":"PubMed","id":"24675744"},{"database":"DOI","id":"10.1371/journal.ppat.1004007"}],"title":"A cytosolic chaperone complexes with dynamic membrane J-proteins and mobilizes a nonenveloped virus out of the endoplasmic reticulum.","publicationDate":"2014","journal":"PLoS Pathog.","firstPage":"E1004007","lastPage":"E1004007","volume":"10"},"referencePositions":["FUNCTION (MICROBIAL INFECTION)","IDENTIFICATION IN A COMPLEX WITH DNAJB14"]},{"referenceNumber":11,"citation":{"id":"25944712","citationType":"journal article","authors":["Vaca Jacome A.S.","Rabilloud T.","Schaeffer-Reiss C.","Rompais M.","Ayoub D.","Lane L.","Bairoch A.","Van Dorsselaer A.","Carapito C."],"citationCrossReferences":[{"database":"PubMed","id":"25944712"},{"database":"DOI","id":"10.1002/pmic.201400617"}],"title":"N-terminome analysis of the human mitochondrial proteome.","publicationDate":"2015","journal":"Proteomics","firstPage":"2519","lastPage":"2524","volume":"15"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]},{"referenceNumber":12,"citation":{"id":"27916661","citationType":"journal article","authors":["Li K.","Jiang Q.","Bai X.","Yang Y.F.","Ruan M.Y.","Cai S.Q."],"citationCrossReferences":[{"database":"PubMed","id":"27916661"},{"database":"DOI","id":"10.1016/j.molcel.2016.10.027"}],"title":"Tetrameric assembly of K(+) channels requires ER-located chaperone proteins.","publicationDate":"2017","journal":"Mol. Cell","firstPage":"52","lastPage":"65","volume":"65"},"referencePositions":["FUNCTION","SUBUNIT","SUBCELLULAR LOCATION","MUTAGENESIS OF 184-GLY--PHE-212"]},{"referenceNumber":13,"citation":{"id":"33563959","citationType":"journal article","authors":["Davydova E.","Shimazu T.","Schuhmacher M.K.","Jakobsson M.E.","Willemen H.L.D.M.","Liu T.","Moen A.","Ho A.Y.Y.","Malecki J.","Schroer L.","Pinto R.","Suzuki T.","Groensberg I.A.","Sohtome Y.","Akakabe M.","Weirich S.","Kikuchi M.","Olsen J.V.","Dohmae N.","Umehara T.","Sodeoka M.","Siino V.","McDonough M.A.","Eijkelkamp N.","Schofield C.J.","Jeltsch A.","Shinkai Y.","Falnes P.O."],"citationCrossReferences":[{"database":"PubMed","id":"33563959"},{"database":"DOI","id":"10.1038/s41467-020-20670-7"}],"title":"The methyltransferase METTL9 mediates pervasive 1-methylhistidine modification in mammalian proteomes.","publicationDate":"2021","journal":"Nat. Commun.","firstPage":"891","lastPage":"891","volume":"12"},"referencePositions":["METHYLATION AT HIS-185","MUTAGENESIS OF HIS-185"]},{"referenceNumber":14,"citation":{"id":"25631089","citationType":"journal article","authors":["Bagchi P.","Walczak C.P.","Tsai B."],"citationCrossReferences":[{"database":"PubMed","id":"25631089"},{"database":"DOI","id":"10.1128/jvi.03574-14"}],"title":"The endoplasmic reticulum membrane J protein C18 executes a distinct role in promoting simian virus 40 membrane penetration.","publicationDate":"2015","journal":"J. Virol.","firstPage":"4058","lastPage":"4068","volume":"89"},"referencePositions":["SUBCELLULAR LOCATION (MICROBIAL INFECTION)"]},{"referenceNumber":15,"citation":{"id":"CI-8TOGC9RUE7SMR","citationType":"submission","authoringGroup":["RIKEN structural genomics initiative (RSGI)"],"title":"Solution structure of J-domain from human DnaJ subfamily B member 12.","publicationDate":"NOV-2005","submissionDatabase":"PDB data bank"},"referencePositions":["STRUCTURE BY NMR OF 110-174"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AK000034","properties":[{"key":"ProteinId","value":"BAA90896.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK025942","properties":[{"key":"ProteinId","value":"BAB15289.1"},{"key":"Status","value":"ALT_INIT"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK302071","properties":[{"key":"ProteinId","value":"BAH13619.1"},{"key":"Status","value":"ALT_INIT"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AC091769","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"BC011812","properties":[{"key":"ProteinId","value":"AAH11812.2"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC064920","properties":[{"key":"ProteinId","value":"AAH64920.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"CCDS","id":"CCDS7316.3","properties":[{"key":"Description","value":"-"}],"isoformId":"Q9NXW2-1"},{"database":"RefSeq","id":"NP_001002762.3","properties":[{"key":"NucleotideSequenceId","value":"NM_001002762.5"}],"isoformId":"Q9NXW2-1"},{"database":"RefSeq","id":"NP_001352009.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001365080.3"}],"isoformId":"Q9NXW2-2"},{"database":"RefSeq","id":"NP_001352010.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001365081.3"}],"isoformId":"Q9NXW2-1"},{"database":"RefSeq","id":"NP_060096.4","properties":[{"key":"NucleotideSequenceId","value":"NM_017626.7"}],"isoformId":"Q9NXW2-1"},{"database":"PDB","id":"2CTP","properties":[{"key":"Method","value":"NMR"},{"key":"Resolution","value":"-"},{"key":"Chains","value":"A=110-174"}]},{"database":"PDBsum","id":"2CTP","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"Q9NXW2","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9NXW2","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"120150","properties":[{"key":"Interactions","value":"202"}]},{"database":"CORUM","id":"Q9NXW2","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q9NXW2","properties":[{"key":"Number of interactors","value":"2407"}]},{"database":"IntAct","id":"Q9NXW2","properties":[{"key":"Interactions","value":"62"}]},{"database":"MINT","id":"Q9NXW2","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"9606.ENSP00000345575","properties":[{"key":"Description","value":"-"}]},{"database":"BindingDB","id":"Q9NXW2","properties":[{"key":"Description","value":"-"}]},{"database":"ChEMBL","id":"CHEMBL6066345","properties":[{"key":"Description","value":"-"}]},{"database":"TCDB","id":"1.P.1.1.1","properties":[{"key":"FamilyName","value":"the polyoma virus sv40 er penetration channel (vpec) family"}]},{"database":"iPTMnet","id":"Q9NXW2","properties":[{"key":"Description","value":"-"}]},{"database":"PhosphoSitePlus","id":"Q9NXW2","properties":[{"key":"Description","value":"-"}]},{"database":"BioMuta","id":"DNAJB12","properties":[{"key":"Description","value":"-"}]},{"database":"DMDM","id":"294862531","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"Q9NXW2","properties":[{"key":"Description","value":"-"}]},{"database":"MassIVE","id":"Q9NXW2","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"9606-ENSP00000345575","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"Q9NXW2","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"83139","properties":[{"key":"Description","value":"-"}],"isoformId":"Q9NXW2-1"},{"database":"ProteomicsDB","id":"83140","properties":[{"key":"Description","value":"-"}],"isoformId":"Q9NXW2-2"},{"database":"Pumba","id":"Q9NXW2","properties":[{"key":"Description","value":"-"}]},{"database":"Antibodypedia","id":"2545","properties":[{"key":"antibodies","value":"177 antibodies from 26 providers"}]},{"database":"DNASU","id":"54788","properties":[{"key":"Description","value":"-"}]},{"database":"Ensembl","id":"ENST00000444643.8","properties":[{"key":"ProteinId","value":"ENSP00000403313.2"},{"key":"GeneId","value":"ENSG00000148719.17"}],"isoformId":"Q9NXW2-1"},{"database":"Ensembl","id":"ENST00000876766.1","properties":[{"key":"ProteinId","value":"ENSP00000546825.1"},{"key":"GeneId","value":"ENSG00000148719.17"}],"isoformId":"Q9NXW2-1"},{"database":"GeneID","id":"54788","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"hsa:54788","properties":[{"key":"Description","value":"-"}]},{"database":"MANE-Select","id":"ENST00000444643.8","properties":[{"key":"ProteinId","value":"ENSP00000403313.2"},{"key":"RefSeqNucleotideId","value":"NM_017626.7"},{"key":"RefSeqProteinId","value":"NP_060096.4"}]},{"database":"UCSC","id":"uc057tyc.1","properties":[{"key":"OrganismName","value":"human"}],"isoformId":"Q9NXW2-1"},{"database":"AGR","id":"HGNC:14891","properties":[{"key":"Description","value":"-"}]},{"database":"ClinPGx","id":"PA27414","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"54788","properties":[{"key":"Description","value":"-"}]},{"database":"DisGeNET","id":"54788","properties":[{"key":"Description","value":"-"}]},{"database":"GeneCards","id":"DNAJB12","properties":[{"key":"Description","value":"-"}]},{"database":"HGNC","id":"HGNC:14891","properties":[{"key":"GeneName","value":"DNAJB12"}]},{"database":"HPA","id":"ENSG00000148719","properties":[{"key":"ExpressionPatterns","value":"Low tissue specificity"}]},{"database":"MIM","id":"608376","properties":[{"key":"Type","value":"gene"}]},{"database":"OpenTargets","id":"ENSG00000148719","properties":[{"key":"Description","value":"-"}]},{"database":"VEuPathDB","id":"HostDB:ENSG00000148719","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG0714","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"GeneTree","id":"ENSGT00940000155590","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q9NXW2","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"442087at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PAN-GO","id":"Q9NXW2","properties":[{"key":"Number of GO annotations","value":"4 GO annotations based on evolutionary models"}]},{"database":"PhylomeDB","id":"Q9NXW2","properties":[{"key":"Description","value":"-"}]},{"database":"PathwayCommons","id":"Q9NXW2","properties":[{"key":"Description","value":"-"}]},{"database":"SignaLink","id":"Q9NXW2","properties":[{"key":"Description","value":"-"}]},{"database":"SIGNOR","id":"Q9NXW2","properties":[{"key":"Description","value":"-"}]},{"database":"Agora","id":"ENSG00000148719","properties":[{"key":"NominatedTarget","value":"-"}]},{"database":"BioGRID-ORCS","id":"54788","properties":[{"key":"hits","value":"65 hits in 1151 CRISPR screens"}]},{"database":"ChiTaRS","id":"DNAJB12","properties":[{"key":"OrganismName","value":"human"}]},{"database":"EvolutionaryTrace","id":"Q9NXW2","properties":[{"key":"Description","value":"-"}]},{"database":"GenomeRNAi","id":"54788","properties":[{"key":"Description","value":"-"}]},{"database":"Pharos","id":"Q9NXW2","properties":[{"key":"DevelopmentLevel","value":"Tbio"}]},{"database":"PRO","id":"PR:Q9NXW2","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000005640","properties":[{"key":"Component","value":"Chromosome 10"}]},{"database":"RNAct","id":"Q9NXW2","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"Bgee","id":"ENSG00000148719","properties":[{"key":"ExpressionPatterns","value":"Expressed in tongue squamous epithelium and 208 other cell types or tissues"}]},{"database":"ExpressionAtlas","id":"Q9NXW2","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0005783","properties":[{"key":"GoTerm","value":"C:endoplasmic reticulum"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"27916661"}]},{"database":"GO","id":"GO:0005789","properties":[{"key":"GoTerm","value":"C:endoplasmic reticulum membrane"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"21148293"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"21150129"}]},{"database":"GO","id":"GO:0016020","properties":[{"key":"GoTerm","value":"C:membrane"},{"key":"GoEvidenceType","value":"HDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0007005","source":"PubMed","id":"19946888"}]},{"database":"GO","id":"GO:0031965","properties":[{"key":"GoTerm","value":"C:nuclear membrane"},{"key":"GoEvidenceType","value":"IDA:HPA"}]},{"database":"GO","id":"GO:0030544","properties":[{"key":"GoTerm","value":"F:Hsp70 protein binding"},{"key":"GoEvidenceType","value":"IPI:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000353","source":"PubMed","id":"21148293"},{"evidenceCode":"ECO:0000353","source":"PubMed","id":"21150129"},{"evidenceCode":"ECO:0000353","source":"PubMed","id":"27916661"}]},{"database":"GO","id":"GO:0071218","properties":[{"key":"GoTerm","value":"P:cellular response to misfolded protein"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"21148293"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"21150129"}]},{"database":"GO","id":"GO:0036503","properties":[{"key":"GoTerm","value":"P:ERAD pathway"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"21148293"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"21150129"}]},{"database":"GO","id":"GO:0006457","properties":[{"key":"GoTerm","value":"P:protein folding"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"27916661"}]},{"database":"GO","id":"GO:0065003","properties":[{"key":"GoTerm","value":"P:protein-containing complex assembly"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"27916661"}]},{"database":"CDD","id":"cd06257","properties":[{"key":"EntryName","value":"DnaJ"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.287.110:FF:000004","properties":[{"key":"EntryName","value":"DnaJ (Hsp40) homolog, subfamily B, member 14"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.287.110","properties":[{"key":"EntryName","value":"DnaJ domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR001623","properties":[{"key":"EntryName","value":"DnaJ_domain"}]},{"database":"InterPro","id":"IPR018253","properties":[{"key":"EntryName","value":"DnaJ_domain_CS"}]},{"database":"InterPro","id":"IPR051100","properties":[{"key":"EntryName","value":"DnaJ_subfamily_B/C"}]},{"database":"InterPro","id":"IPR015399","properties":[{"key":"EntryName","value":"DUF1977_DnaJ-like"}]},{"database":"InterPro","id":"IPR036869","properties":[{"key":"EntryName","value":"J_dom_sf"}]},{"database":"PANTHER","id":"PTHR43908","properties":[{"key":"EntryName","value":"AT29763P-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43908:SF8","properties":[{"key":"EntryName","value":"DNAJ HOMOLOG SUBFAMILY B MEMBER 12"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00226","properties":[{"key":"EntryName","value":"DnaJ"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF09320","properties":[{"key":"EntryName","value":"DUF1977"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00625","properties":[{"key":"EntryName","value":"JDOMAIN"}]},{"database":"SMART","id":"SM00271","properties":[{"key":"EntryName","value":"DnaJ"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF46565","properties":[{"key":"EntryName","value":"Chaperone J-domain"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00636","properties":[{"key":"EntryName","value":"DNAJ_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50076","properties":[{"key":"EntryName","value":"DNAJ_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MESNKDEAERCISIALKAIQSNQPDRALRFLEKAQRLYPTPRVRALIESLNQKPQTAGDQPPPTDTTHATHRKAGGTDAPSANGEAGGESTKGYTAEQVAAVKRVKQCKDYYEILGVSRGASDEDLKKAYRRLALKFHPDKNHAPGATEAFKAIGTAYAVLSNPEKRKQYDQFGDDKSQAARHGHGHGDFHRGFEADISPEDLFNMFFGGGFPSSNVHVYSNGRMRYTYQQRQDRRDNQGDGGLGVFVQLMPILILILVSALSQLMVSSPPYSLSPRPSVGHIHRRVTDHLGVVYYVGDTFSEEYTGSSLKTVERNVEDDYIANLRNNCWKEKQQKEGLLYRARYFGDTDMYHRAQKMGTPSCSRLSEVQASLHG","length":375,"molWeight":41860,"crc64":"A9F0C7F3E22EF76F","md5":"C08D628AD8223440C5C8F499E13D8564"},"extraAttributes":{"countByCommentType":{"FUNCTION":2,"SUBUNIT":1,"SUBCELLULAR LOCATION":2,"ALTERNATIVE PRODUCTS":2,"PTM":1,"SIMILARITY":1,"SEQUENCE CAUTION":2},"countByFeatureType":{"Chain":1,"Topological domain":2,"Transmembrane":1,"Domain":1,"Region":1,"Compositional bias":1,"Modified residue":2,"Alternative sequence":1,"Natural variant":1,"Mutagenesis":3,"Sequence conflict":3,"Helix":4,"Turn":1},"uniParcId":"UPI0000049FCE"}}