{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9NVZ3","secondaryAccessions":["B4DY19","E9PGQ8","Q5VSU4","Q5VSU5","Q9H7L1","Q9H8L1"],"uniProtkbId":"NECP2_HUMAN","entryAudit":{"firstPublicDate":"2005-03-29","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2000-10-01","entryVersion":176,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Homo sapiens","commonName":"Human","taxonId":9606,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Primates","Haplorrhini","Catarrhini","Hominidae","Homo"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Adaptin ear-binding coat-associated protein 2"}},"alternativeNames":[{"fullName":{"value":"NECAP endocytosis-associated protein 2"},"shortNames":[{"value":"NECAP-2"}]}]},"genes":[{"geneName":{"value":"NECAP2"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Involved in endocytosis"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Interacts with AP1G1 and AP2A1 components of the adapter protein complexes AP-1 and AP-2. Interacts with the GAE domain proteins GGA1, GGA2 and GGA3 (By similarity)"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"Q9NVZ3","intActId":"EBI-742525"},"interactantTwo":{"uniProtKBAccession":"P63010","geneName":"AP2B1","intActId":"EBI-432924"},"numberOfExperiments":4,"organismDiffer":false}]},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Colocalizes with AP-2 at the plasma membrane"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Cytoplasmic vesicle, clathrin-coated vesicle membrane","id":"SL-0071"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Cell membrane","id":"SL-0039"}}]},{"commentType":"ALTERNATIVE PRODUCTS","events":["Alternative splicing"],"isoforms":[{"name":{"value":"1"},"isoformIds":["Q9NVZ3-1"],"isoformSequenceStatus":"Displayed"},{"name":{"value":"2"},"isoformIds":["Q9NVZ3-2"],"sequenceIds":["VSP_013236"],"isoformSequenceStatus":"Described"},{"name":{"value":"3"},"isoformIds":["Q9NVZ3-3"],"sequenceIds":["VSP_013234","VSP_013235"],"isoformSequenceStatus":"Described"},{"name":{"value":"4"},"isoformIds":["Q9NVZ3-4"],"sequenceIds":["VSP_041726"],"isoformSequenceStatus":"Described"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"The WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif and the properties of the second residue of the motif itself (By similarity)"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the NECAP family"}],"commentType":"SIMILARITY"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"BAB15758.1","note":"Extended N-terminus.","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":263,"modifier":"EXACT"}},"description":"Adaptin ear-binding coat-associated protein 2","featureId":"PRO_0000213071"},{"type":"Region","location":{"start":{"value":166,"modifier":"EXACT"},"end":{"value":194,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":219,"modifier":"EXACT"},"end":{"value":263,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Motif","location":{"start":{"value":240,"modifier":"EXACT"},"end":{"value":243,"modifier":"EXACT"}},"description":"WXXF motif 1"},{"type":"Motif","location":{"start":{"value":260,"modifier":"EXACT"},"end":{"value":263,"modifier":"EXACT"}},"description":"WXXF motif 2"},{"type":"Compositional bias","location":{"start":{"value":246,"modifier":"EXACT"},"end":{"value":263,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Modified residue","location":{"start":{"value":181,"modifier":"EXACT"},"end":{"value":181,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"23186163"}]},{"type":"Alternative sequence","location":{"start":{"value":6,"modifier":"EXACT"},"end":{"value":31,"modifier":"EXACT"}},"description":"in isoform 4","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"14702039"}],"featureId":"VSP_041726","alternativeSequence":{}},{"type":"Alternative sequence","location":{"start":{"value":164,"modifier":"EXACT"},"end":{"value":172,"modifier":"EXACT"}},"description":"in isoform 3","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"11214971"}],"featureId":"VSP_013234","alternativeSequence":{"originalSequence":"NMKKKEGAA","alternativeSequences":["VSSTLAWLW"]}},{"type":"Alternative sequence","location":{"start":{"value":173,"modifier":"EXACT"},"end":{"value":263,"modifier":"EXACT"}},"description":"in isoform 3","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"11214971"}],"featureId":"VSP_013235","alternativeSequence":{}},{"type":"Alternative sequence","location":{"start":{"value":223,"modifier":"EXACT"},"end":{"value":263,"modifier":"EXACT"}},"description":"in isoform 2","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"14702039"}],"featureId":"VSP_013236","alternativeSequence":{"originalSequence":"GGAPVPWPQPNPATADIWGDFTKSTGSTSSQTQPGTGWVQF","alternativeSequences":["DQLPARPSQAQAGSSSDLSTVFPHVTSGKALPHLGQRKEDEALLSWPVFGA"]}},{"type":"Natural variant","location":{"start":{"value":149,"modifier":"EXACT"},"end":{"value":149,"modifier":"EXACT"}},"description":"in dbSNP:rs35056694","featureCrossReferences":[{"database":"dbSNP","id":"rs35056694"}],"featureId":"VAR_034154","alternativeSequence":{"originalSequence":"D","alternativeSequences":["A"]}},{"type":"Sequence conflict","location":{"start":{"value":192,"modifier":"EXACT"},"end":{"value":192,"modifier":"EXACT"}},"description":"in Ref. 2; BAG63581","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"P","alternativeSequences":["S"]}}],"keywords":[{"id":"KW-0025","category":"Coding sequence diversity","name":"Alternative splicing"},{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0968","category":"Cellular component","name":"Cytoplasmic vesicle"},{"id":"KW-0254","category":"Biological process","name":"Endocytosis"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-0653","category":"Biological process","name":"Protein transport"},{"id":"KW-1267","category":"Technical term","name":"Proteomics identification"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0677","category":"Domain","name":"Repeat"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"11214971","citationType":"journal article","authors":["Hattori A.","Okumura K.","Nagase T.","Kikuno R.","Hirosawa M.","Ohara O."],"citationCrossReferences":[{"database":"PubMed","id":"11214971"},{"database":"DOI","id":"10.1093/dnares/7.6.357"}],"title":"Characterization of long cDNA clones from human adult spleen.","publicationDate":"2000","journal":"DNA Res.","firstPage":"357","lastPage":"366","volume":"7"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3)"],"referenceComments":[{"value":"Spleen","type":"TISSUE"}]},{"referenceNumber":2,"citation":{"id":"14702039","citationType":"journal article","authors":["Ota T.","Suzuki Y.","Nishikawa T.","Otsuki T.","Sugiyama T.","Irie R.","Wakamatsu A.","Hayashi K.","Sato H.","Nagai K.","Kimura K.","Makita H.","Sekine M.","Obayashi M.","Nishi T.","Shibahara T.","Tanaka T.","Ishii S.","Yamamoto J.","Saito K.","Kawai Y.","Isono Y.","Nakamura Y.","Nagahari K.","Murakami K.","Yasuda T.","Iwayanagi T.","Wagatsuma M.","Shiratori A.","Sudo H.","Hosoiri T.","Kaku Y.","Kodaira H.","Kondo H.","Sugawara M.","Takahashi M.","Kanda K.","Yokoi T.","Furuya T.","Kikkawa E.","Omura Y.","Abe K.","Kamihara K.","Katsuta N.","Sato K.","Tanikawa M.","Yamazaki M.","Ninomiya K.","Ishibashi T.","Yamashita H.","Murakawa K.","Fujimori K.","Tanai H.","Kimata M.","Watanabe M.","Hiraoka S.","Chiba Y.","Ishida S.","Ono Y.","Takiguchi S.","Watanabe S.","Yosida M.","Hotuta T.","Kusano J.","Kanehori K.","Takahashi-Fujii A.","Hara H.","Tanase T.-O.","Nomura Y.","Togiya S.","Komai F.","Hara R.","Takeuchi K.","Arita M.","Imose N.","Musashino K.","Yuuki H.","Oshima A.","Sasaki N.","Aotsuka S.","Yoshikawa Y.","Matsunawa H.","Ichihara T.","Shiohata N.","Sano S.","Moriya S.","Momiyama H.","Satoh N.","Takami S.","Terashima Y.","Suzuki O.","Nakagawa S.","Senoh A.","Mizoguchi H.","Goto Y.","Shimizu F.","Wakebe H.","Hishigaki H.","Watanabe T.","Sugiyama A.","Takemoto M.","Kawakami B.","Yamazaki M.","Watanabe K.","Kumagai A.","Itakura S.","Fukuzumi Y.","Fujimori Y.","Komiyama M.","Tashiro H.","Tanigami A.","Fujiwara T.","Ono T.","Yamada K.","Fujii Y.","Ozaki K.","Hirao M.","Ohmori Y.","Kawabata A.","Hikiji T.","Kobatake N.","Inagaki H.","Ikema Y.","Okamoto S.","Okitani R.","Kawakami T.","Noguchi S.","Itoh T.","Shigeta K.","Senba T.","Matsumura K.","Nakajima Y.","Mizuno T.","Morinaga M.","Sasaki M.","Togashi T.","Oyama M.","Hata H.","Watanabe M.","Komatsu T.","Mizushima-Sugano J.","Satoh T.","Shirai Y.","Takahashi Y.","Nakagawa K.","Okumura K.","Nagase T.","Nomura N.","Kikuchi H.","Masuho Y.","Yamashita R.","Nakai K.","Yada T.","Nakamura Y.","Ohara O.","Isogai T.","Sugano S."],"citationCrossReferences":[{"database":"PubMed","id":"14702039"},{"database":"DOI","id":"10.1038/ng1285"}],"title":"Complete sequencing and characterization of 21,243 full-length human cDNAs.","publicationDate":"2004","journal":"Nat. Genet.","firstPage":"40","lastPage":"45","volume":"36"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 4)"],"referenceComments":[{"value":"Placenta","type":"TISSUE"},{"value":"Spleen","type":"TISSUE"},{"value":"Testis","type":"TISSUE"}]},{"referenceNumber":3,"citation":{"id":"16710414","citationType":"journal article","authors":["Gregory S.G.","Barlow K.F.","McLay K.E.","Kaul R.","Swarbreck D.","Dunham A.","Scott C.E.","Howe K.L.","Woodfine K.","Spencer C.C.A.","Jones M.C.","Gillson C.","Searle S.","Zhou Y.","Kokocinski F.","McDonald L.","Evans R.","Phillips K.","Atkinson A.","Cooper R.","Jones C.","Hall R.E.","Andrews T.D.","Lloyd C.","Ainscough R.","Almeida J.P.","Ambrose K.D.","Anderson F.","Andrew R.W.","Ashwell R.I.S.","Aubin K.","Babbage A.K.","Bagguley C.L.","Bailey J.","Beasley H.","Bethel G.","Bird C.P.","Bray-Allen S.","Brown J.Y.","Brown A.J.","Buckley D.","Burton J.","Bye J.","Carder C.","Chapman J.C.","Clark S.Y.","Clarke G.","Clee C.","Cobley V.","Collier R.E.","Corby N.","Coville G.J.","Davies J.","Deadman R.","Dunn M.","Earthrowl M.","Ellington A.G.","Errington H.","Frankish A.","Frankland J.","French L.","Garner P.","Garnett J.","Gay L.","Ghori M.R.J.","Gibson R.","Gilby L.M.","Gillett W.","Glithero R.J.","Grafham D.V.","Griffiths C.","Griffiths-Jones S.","Grocock R.","Hammond S.","Harrison E.S.I.","Hart E.","Haugen E.","Heath P.D.","Holmes S.","Holt K.","Howden P.J.","Hunt A.R.","Hunt S.E.","Hunter G.","Isherwood J.","James R.","Johnson C.","Johnson D.","Joy A.","Kay M.","Kershaw J.K.","Kibukawa M.","Kimberley A.M.","King A.","Knights A.J.","Lad H.","Laird G.","Lawlor S.","Leongamornlert D.A.","Lloyd D.M.","Loveland J.","Lovell J.","Lush M.J.","Lyne R.","Martin S.","Mashreghi-Mohammadi M.","Matthews L.","Matthews N.S.W.","McLaren S.","Milne S.","Mistry S.","Moore M.J.F.","Nickerson T.","O'Dell C.N.","Oliver K.","Palmeiri A.","Palmer S.A.","Parker A.","Patel D.","Pearce A.V.","Peck A.I.","Pelan S.","Phelps K.","Phillimore B.J.","Plumb R.","Rajan J.","Raymond C.","Rouse G.","Saenphimmachak C.","Sehra H.K.","Sheridan E.","Shownkeen R.","Sims S.","Skuce C.D.","Smith M.","Steward C.","Subramanian S.","Sycamore N.","Tracey A.","Tromans A.","Van Helmond Z.","Wall M.","Wallis J.M.","White S.","Whitehead S.L.","Wilkinson J.E.","Willey D.L.","Williams H.","Wilming L.","Wray P.W.","Wu Z.","Coulson A.","Vaudin M.","Sulston J.E.","Durbin R.M.","Hubbard T.","Wooster R.","Dunham I.","Carter N.P.","McVean G.","Ross M.T.","Harrow J.","Olson M.V.","Beck S.","Rogers J.","Bentley D.R."],"citationCrossReferences":[{"database":"PubMed","id":"16710414"},{"database":"DOI","id":"10.1038/nature04727"}],"title":"The DNA sequence and biological annotation of human chromosome 1.","publicationDate":"2006","journal":"Nature","firstPage":"315","lastPage":"321","volume":"441"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"]},{"referenceNumber":4,"citation":{"id":"15489334","citationType":"journal article","authoringGroup":["The MGC Project Team"],"citationCrossReferences":[{"database":"PubMed","id":"15489334"},{"database":"DOI","id":"10.1101/gr.2596504"}],"title":"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).","publicationDate":"2004","journal":"Genome Res.","firstPage":"2121","lastPage":"2127","volume":"14"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)"],"referenceComments":[{"value":"Colon","type":"TISSUE"},{"value":"Eye","type":"TISSUE"}]},{"referenceNumber":5,"citation":{"id":"18669648","citationType":"journal article","authors":["Dephoure N.","Zhou C.","Villen J.","Beausoleil S.A.","Bakalarski C.E.","Elledge S.J.","Gygi S.P."],"citationCrossReferences":[{"database":"PubMed","id":"18669648"},{"database":"DOI","id":"10.1073/pnas.0805139105"}],"title":"A quantitative atlas of mitotic phosphorylation.","publicationDate":"2008","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"10762","lastPage":"10767","volume":"105"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"Cervix carcinoma","type":"TISSUE"}]},{"referenceNumber":6,"citation":{"id":"21269460","citationType":"journal article","authors":["Burkard T.R.","Planyavsky M.","Kaupe I.","Breitwieser F.P.","Buerckstuemmer T.","Bennett K.L.","Superti-Furga G.","Colinge J."],"citationCrossReferences":[{"database":"PubMed","id":"21269460"},{"database":"DOI","id":"10.1186/1752-0509-5-17"}],"title":"Initial characterization of the human central proteome.","publicationDate":"2011","journal":"BMC Syst. Biol.","firstPage":"17","lastPage":"17","volume":"5"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]},{"referenceNumber":7,"citation":{"id":"23186163","citationType":"journal article","authors":["Zhou H.","Di Palma S.","Preisinger C.","Peng M.","Polat A.N.","Heck A.J.","Mohammed S."],"citationCrossReferences":[{"database":"PubMed","id":"23186163"},{"database":"DOI","id":"10.1021/pr300630k"}],"title":"Toward a comprehensive characterization of a human cancer cell phosphoproteome.","publicationDate":"2013","journal":"J. Proteome Res.","firstPage":"260","lastPage":"271","volume":"12"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-181","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"Cervix carcinoma","type":"TISSUE"},{"value":"Erythroleukemia","type":"TISSUE"}]},{"referenceNumber":8,"citation":{"id":"24275569","citationType":"journal article","authors":["Bian Y.","Song C.","Cheng K.","Dong M.","Wang F.","Huang J.","Sun D.","Wang L.","Ye M.","Zou H."],"citationCrossReferences":[{"database":"PubMed","id":"24275569"},{"database":"DOI","id":"10.1016/j.jprot.2013.11.014"}],"title":"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.","publicationDate":"2014","journal":"J. Proteomics","firstPage":"253","lastPage":"262","volume":"96"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"Liver","type":"TISSUE"}]},{"referenceNumber":9,"citation":{"id":"25944712","citationType":"journal article","authors":["Vaca Jacome A.S.","Rabilloud T.","Schaeffer-Reiss C.","Rompais M.","Ayoub D.","Lane L.","Bairoch A.","Van Dorsselaer A.","Carapito C."],"citationCrossReferences":[{"database":"PubMed","id":"25944712"},{"database":"DOI","id":"10.1002/pmic.201400617"}],"title":"N-terminome analysis of the human mitochondrial proteome.","publicationDate":"2015","journal":"Proteomics","firstPage":"2519","lastPage":"2524","volume":"15"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AK024468","properties":[{"key":"ProteinId","value":"BAB15758.1"},{"key":"Status","value":"ALT_INIT"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK302227","properties":[{"key":"ProteinId","value":"BAG63581.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK001282","properties":[{"key":"ProteinId","value":"BAA91598.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK023545","properties":[{"key":"ProteinId","value":"BAB14605.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AL137802","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AL669962","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"BC017014","properties":[{"key":"ProteinId","value":"AAH17014.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC018914","properties":[{"key":"ProteinId","value":"AAH18914.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"CCDS","id":"CCDS173.1","properties":[{"key":"Description","value":"-"}],"isoformId":"Q9NVZ3-1"},{"database":"CCDS","id":"CCDS44066.1","properties":[{"key":"Description","value":"-"}],"isoformId":"Q9NVZ3-2"},{"database":"CCDS","id":"CCDS44067.1","properties":[{"key":"Description","value":"-"}],"isoformId":"Q9NVZ3-4"},{"database":"RefSeq","id":"NP_001138749.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001145277.2"}],"isoformId":"Q9NVZ3-2"},{"database":"RefSeq","id":"NP_001138750.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001145278.2"}],"isoformId":"Q9NVZ3-4"},{"database":"RefSeq","id":"NP_060560.1","properties":[{"key":"NucleotideSequenceId","value":"NM_018090.5"}],"isoformId":"Q9NVZ3-1"},{"database":"AlphaFoldDB","id":"Q9NVZ3","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9NVZ3","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"120832","properties":[{"key":"Interactions","value":"69"}]},{"database":"FunCoup","id":"Q9NVZ3","properties":[{"key":"Number of interactors","value":"2236"}]},{"database":"IntAct","id":"Q9NVZ3","properties":[{"key":"Interactions","value":"44"}]},{"database":"NDEx","id":"MUSIC2-C5241-NECAP2","properties":[{"key":"Evidences","value":"Osteosarcoma (U2OS) cell map - Endocytic pathway regulation and vesicle trafficking (16 proteins)"}]},{"database":"STRING","id":"9606.ENSP00000391942","properties":[{"key":"Description","value":"-"}]},{"database":"GlyGen","id":"Q9NVZ3","properties":[{"key":"glycosylation","value":"1 site, 1 O-linked glycan (1 site)"}]},{"database":"iPTMnet","id":"Q9NVZ3","properties":[{"key":"Description","value":"-"}]},{"database":"MetOSite","id":"Q9NVZ3","properties":[{"key":"Description","value":"-"}]},{"database":"PhosphoSitePlus","id":"Q9NVZ3","properties":[{"key":"Description","value":"-"}]},{"database":"BioMuta","id":"NECAP2","properties":[{"key":"Description","value":"-"}]},{"database":"DMDM","id":"62287168","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"Q9NVZ3","properties":[{"key":"Description","value":"-"}]},{"database":"MassIVE","id":"Q9NVZ3","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"9606-ENSP00000391942","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"Q9NVZ3","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"82880","properties":[{"key":"Description","value":"-"}],"isoformId":"Q9NVZ3-1"},{"database":"ProteomicsDB","id":"82881","properties":[{"key":"Description","value":"-"}],"isoformId":"Q9NVZ3-2"},{"database":"ProteomicsDB","id":"82882","properties":[{"key":"Description","value":"-"}],"isoformId":"Q9NVZ3-3"},{"database":"ProteomicsDB","id":"82883","properties":[{"key":"Description","value":"-"}],"isoformId":"Q9NVZ3-4"},{"database":"Pumba","id":"Q9NVZ3","properties":[{"key":"Description","value":"-"}]},{"database":"Antibodypedia","id":"29202","properties":[{"key":"antibodies","value":"60 antibodies from 22 providers"}]},{"database":"DNASU","id":"55707","properties":[{"key":"Description","value":"-"}]},{"database":"Ensembl","id":"ENST00000337132.10","properties":[{"key":"ProteinId","value":"ENSP00000338746.5"},{"key":"GeneId","value":"ENSG00000157191.21"}],"isoformId":"Q9NVZ3-1"},{"database":"Ensembl","id":"ENST00000443980.6","properties":[{"key":"ProteinId","value":"ENSP00000391942.2"},{"key":"GeneId","value":"ENSG00000157191.21"}],"isoformId":"Q9NVZ3-2"},{"database":"Ensembl","id":"ENST00000457722.6","properties":[{"key":"ProteinId","value":"ENSP00000407091.2"},{"key":"GeneId","value":"ENSG00000157191.21"}],"isoformId":"Q9NVZ3-4"},{"database":"Ensembl","id":"ENST00000492095.5","properties":[{"key":"ProteinId","value":"ENSP00000427620.1"},{"key":"GeneId","value":"ENSG00000157191.21"}],"isoformId":"Q9NVZ3-1"},{"database":"Ensembl","id":"ENST00000707372.1","properties":[{"key":"ProteinId","value":"ENSP00000516847.1"},{"key":"GeneId","value":"ENSG00000291380.1"}],"isoformId":"Q9NVZ3-1"},{"database":"Ensembl","id":"ENST00000707374.1","properties":[{"key":"ProteinId","value":"ENSP00000516849.1"},{"key":"GeneId","value":"ENSG00000291380.1"}],"isoformId":"Q9NVZ3-4"},{"database":"Ensembl","id":"ENST00000707376.1","properties":[{"key":"ProteinId","value":"ENSP00000516850.1"},{"key":"GeneId","value":"ENSG00000291380.1"}],"isoformId":"Q9NVZ3-2"},{"database":"Ensembl","id":"ENST00000707379.1","properties":[{"key":"ProteinId","value":"ENSP00000516852.1"},{"key":"GeneId","value":"ENSG00000291380.1"}],"isoformId":"Q9NVZ3-1"},{"database":"GeneID","id":"55707","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"hsa:55707","properties":[{"key":"Description","value":"-"}]},{"database":"MANE-Select","id":"ENST00000337132.10","properties":[{"key":"ProteinId","value":"ENSP00000338746.5"},{"key":"RefSeqNucleotideId","value":"NM_018090.5"},{"key":"RefSeqProteinId","value":"NP_060560.1"}]},{"database":"UCSC","id":"uc001ayo.4","properties":[{"key":"OrganismName","value":"human"}],"isoformId":"Q9NVZ3-1"},{"database":"AGR","id":"HGNC:25528","properties":[{"key":"Description","value":"-"}]},{"database":"ClinPGx","id":"PA142671268","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"55707","properties":[{"key":"Description","value":"-"}]},{"database":"DisGeNET","id":"55707","properties":[{"key":"Description","value":"-"}]},{"database":"GeneCards","id":"NECAP2","properties":[{"key":"Description","value":"-"}]},{"database":"HGNC","id":"HGNC:25528","properties":[{"key":"GeneName","value":"NECAP2"}]},{"database":"HPA","id":"ENSG00000157191","properties":[{"key":"ExpressionPatterns","value":"Low tissue specificity"}]},{"database":"MalaCards","id":"NECAP2","properties":[{"key":"Description","value":"-"}]},{"database":"MIM","id":"611624","properties":[{"key":"Type","value":"gene"}]},{"database":"OpenTargets","id":"ENSG00000157191","properties":[{"key":"Description","value":"-"}]},{"database":"VEuPathDB","id":"HostDB:ENSG00000157191","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG2500","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"GeneTree","id":"ENSGT00390000009359","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_069884_1_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q9NVZ3","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"NEGHRAQ","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"10265489at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PAN-GO","id":"Q9NVZ3","properties":[{"key":"Number of GO annotations","value":"2 GO annotations based on evolutionary models"}]},{"database":"PhylomeDB","id":"Q9NVZ3","properties":[{"key":"Description","value":"-"}]},{"database":"PathwayCommons","id":"Q9NVZ3","properties":[{"key":"Description","value":"-"}]},{"database":"Reactome","id":"R-HSA-8856825","properties":[{"key":"PathwayName","value":"Cargo recognition for clathrin-mediated endocytosis"}]},{"database":"Reactome","id":"R-HSA-8856828","properties":[{"key":"PathwayName","value":"Clathrin-mediated endocytosis"}]},{"database":"SignaLink","id":"Q9NVZ3","properties":[{"key":"Description","value":"-"}]},{"database":"Agora","id":"ENSG00000157191","properties":[{"key":"NominatedTarget","value":"-"}]},{"database":"BioGRID-ORCS","id":"55707","properties":[{"key":"hits","value":"8 hits in 1152 CRISPR screens"}]},{"database":"ChiTaRS","id":"NECAP2","properties":[{"key":"OrganismName","value":"human"}]},{"database":"GeneWiki","id":"NECAP2","properties":[{"key":"Description","value":"-"}]},{"database":"GenomeRNAi","id":"55707","properties":[{"key":"Description","value":"-"}]},{"database":"Pharos","id":"Q9NVZ3","properties":[{"key":"DevelopmentLevel","value":"Tdark"}]},{"database":"PRO","id":"PR:Q9NVZ3","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000005640","properties":[{"key":"Component","value":"Chromosome 1"}]},{"database":"RNAct","id":"Q9NVZ3","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"Bgee","id":"ENSG00000157191","properties":[{"key":"ExpressionPatterns","value":"Expressed in oocyte and 179 other cell types or tissues"}]},{"database":"ExpressionAtlas","id":"Q9NVZ3","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0030125","properties":[{"key":"GoTerm","value":"C:clathrin vesicle coat"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0005905","properties":[{"key":"GoTerm","value":"C:clathrin-coated pit"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0006897","properties":[{"key":"GoTerm","value":"P:endocytosis"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0016192","properties":[{"key":"GoTerm","value":"P:vesicle-mediated transport"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"CDD","id":"cd13228","properties":[{"key":"EntryName","value":"PHear_NECAP"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.30.29.30:FF:000064","properties":[{"key":"EntryName","value":"Adaptin ear-binding coat-associated protein 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.30.29.30","properties":[{"key":"EntryName","value":"Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR012466","properties":[{"key":"EntryName","value":"NECAP_PHear"}]},{"database":"InterPro","id":"IPR011993","properties":[{"key":"EntryName","value":"PH-like_dom_sf"}]},{"database":"PANTHER","id":"PTHR12847:SF16","properties":[{"key":"EntryName","value":"ADAPTIN EAR-BINDING COAT-ASSOCIATED PROTEIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12847","properties":[{"key":"EntryName","value":"ATP-BINDING CASSETTE ABC TRANSPORTER-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF07933","properties":[{"key":"EntryName","value":"DUF1681"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF50729","properties":[{"key":"EntryName","value":"PH domain-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MEESGYESVLCVKPDVHVYRIPPRATNRGYRAAEWQLDQPSWSGRLRITAKGQMAYIKLEDRTSGELFAQAPVDQFPGTAVESVTDSSRYFVIRIEDGNGRRAFIGIGFGDRGDAFDFNVALQDHFKWVKQQCEFAKQAQNPDQGPKLDLGFKEGQTIKLNIANMKKKEGAAGNPRVRPASTGGLSLLPPPPGGKTSTLIPPPGEQLAVGGSLVQPAVAPSSGGAPVPWPQPNPATADIWGDFTKSTGSTSSQTQPGTGWVQF","length":263,"molWeight":28339,"crc64":"6F4DE1B8335852D4","md5":"E2EBFE061AD3B73D5611CBB833234072"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"INTERACTION":1,"SUBCELLULAR LOCATION":1,"ALTERNATIVE PRODUCTS":4,"DOMAIN":1,"SIMILARITY":1,"SEQUENCE CAUTION":1},"countByFeatureType":{"Chain":1,"Region":2,"Motif":2,"Compositional bias":1,"Modified residue":1,"Alternative sequence":4,"Natural variant":1,"Sequence conflict":1},"uniParcId":"UPI000006F33F"}}