ID   OLFL3_HUMAN             Reviewed;         406 AA.
AC   Q9NRN5; Q53FR1; Q53HV9; Q5SQL6; Q69AX9; Q8NBJ2;
DT   24-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   27-MAR-2024, entry version 167.
DE   RecName: Full=Olfactomedin-like protein 3;
DE   AltName: Full=HNOEL-iso;
DE   AltName: Full=hOLF44;
DE   Flags: Precursor;
GN   Name=OLFML3; ORFNames=PSEC0035, PSEC0173, PSEC0244, UNQ663/PRO1294;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE
RP   SPECIFICITY, AND FUNCTION.
RX   PubMed=15280020; DOI=10.1016/j.febslet.2004.06.059;
RA   Zeng L.-C., Liu F., Zhang X., Zhu Z.-D., Wang Z.-Q., Han Z.-G., Ma W.-J.;
RT   "hOLF44, a secreted glycoprotein with distinct expression pattern, belongs
RT   to an uncharacterized olfactomedin-like subfamily newly identified by
RT   phylogenetic analysis.";
RL   FEBS Lett. 571:74-80(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Dendritic cell;
RA   Huang C., Ren S., Tu Y., Gu W., Wang Y., Han Z., Chen Z.;
RT   "Novel genes expressed in human dendritic cells.";
RL   Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX   PubMed=12975309; DOI=10.1101/gr.1293003;
RA   Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA   Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA   Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA   Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA   Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA   Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA   Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA   Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT   "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT   identify novel human secreted and transmembrane proteins: a bioinformatics
RT   assessment.";
RL   Genome Res. 13:2265-2270(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Adipose tissue, and Gastric mucosa;
RA   Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y.,
RA   Tanaka A., Yokoyama S.;
RL   Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Teratocarcinoma;
RX   PubMed=16303743; DOI=10.1093/dnares/12.2.117;
RA   Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J.,
RA   Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S.,
RA   Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.,
RA   Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S.,
RA   Isogai T.;
RT   "Signal sequence and keyword trap in silico for selection of full-length
RT   human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA
RT   libraries.";
RL   DNA Res. 12:117-126(2005).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16710414; DOI=10.1038/nature04727;
RA   Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA   Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA   Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA   Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA   Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA   Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA   Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA   Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA   Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA   Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA   Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA   Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA   Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA   Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA   Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA   Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA   Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA   Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA   Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA   McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA   Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA   Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA   Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA   Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA   Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA   White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA   Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA   Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA   Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT   "The DNA sequence and biological annotation of human chromosome 1.";
RL   Nature 441:315-321(2006).
RN   [8]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Muscle;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Secreted scaffold protein that plays an essential role in
CC       dorsoventral patterning during early development. Stabilizes axial
CC       formation by restricting chordin (CHRD) activity on the dorsal side.
CC       Acts by facilitating the association between the tolloid proteases and
CC       their substrate chordin (CHRD), leading to enhance chordin (CHRD)
CC       degradation (By similarity). May have matrix-related function involved
CC       in placental and embryonic development, or play a similar role in other
CC       physiological processes. {ECO:0000250, ECO:0000269|PubMed:15280020}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:15280020}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=3;
CC       Name=1;
CC         IsoId=Q9NRN5-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q9NRN5-2; Sequence=VSP_014128, VSP_014129;
CC       Name=3;
CC         IsoId=Q9NRN5-3; Sequence=VSP_014130, VSP_014131;
CC   -!- TISSUE SPECIFICITY: Abundant in placenta, moderate in liver and heart,
CC       whereas fairly weak in other tissues examined. On term placenta, mainly
CC       localized extracellularly surrounding the syncytiotrophoblastic cells
CC       and very rarely expressed in the maternal decidua layer.
CC       {ECO:0000269|PubMed:15280020}.
CC   -!- SIMILARITY: Belongs to the OLFML3 family. {ECO:0000305}.
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DR   EMBL; AY464015; AAR88262.1; -; mRNA.
DR   EMBL; AF201945; AAF86881.1; -; mRNA.
DR   EMBL; AY358954; AAQ89313.1; -; mRNA.
DR   EMBL; AK075544; BAC11687.1; -; mRNA.
DR   EMBL; AK223221; BAD96941.1; -; mRNA.
DR   EMBL; AK222471; BAD96191.1; -; mRNA.
DR   EMBL; AK075353; BAC11564.1; -; mRNA.
DR   EMBL; AK075479; BAC11644.1; -; mRNA.
DR   EMBL; AL731797; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC009920; AAH09920.1; -; mRNA.
DR   CCDS; CCDS65618.1; -. [Q9NRN5-2]
DR   CCDS; CCDS870.1; -. [Q9NRN5-1]
DR   RefSeq; NP_001273281.1; NM_001286352.2. [Q9NRN5-2]
DR   RefSeq; NP_064575.1; NM_020190.4. [Q9NRN5-1]
DR   RefSeq; XP_016857337.1; XM_017001848.1. [Q9NRN5-2]
DR   AlphaFoldDB; Q9NRN5; -.
DR   SMR; Q9NRN5; -.
DR   BioGRID; 121268; 13.
DR   IntAct; Q9NRN5; 7.
DR   MINT; Q9NRN5; -.
DR   STRING; 9606.ENSP00000322273; -.
DR   GlyConnect; 1587; 52 N-Linked glycans (2 sites).
DR   GlyCosmos; Q9NRN5; 2 sites, 55 glycans.
DR   GlyGen; Q9NRN5; 3 sites, 55 N-linked glycans (2 sites), 1 O-linked glycan (1 site).
DR   iPTMnet; Q9NRN5; -.
DR   PhosphoSitePlus; Q9NRN5; -.
DR   BioMuta; OLFML3; -.
DR   DMDM; 37999795; -.
DR   EPD; Q9NRN5; -.
DR   jPOST; Q9NRN5; -.
DR   MassIVE; Q9NRN5; -.
DR   PaxDb; 9606-ENSP00000322273; -.
DR   PeptideAtlas; Q9NRN5; -.
DR   ProteomicsDB; 82393; -. [Q9NRN5-1]
DR   ProteomicsDB; 82394; -. [Q9NRN5-2]
DR   ProteomicsDB; 82395; -. [Q9NRN5-3]
DR   ABCD; Q9NRN5; 3 sequenced antibodies.
DR   Antibodypedia; 53757; 88 antibodies from 21 providers.
DR   DNASU; 56944; -.
DR   Ensembl; ENST00000320334.5; ENSP00000322273.4; ENSG00000116774.12. [Q9NRN5-1]
DR   Ensembl; ENST00000369551.5; ENSP00000358564.1; ENSG00000116774.12. [Q9NRN5-2]
DR   GeneID; 56944; -.
DR   KEGG; hsa:56944; -.
DR   MANE-Select; ENST00000320334.5; ENSP00000322273.4; NM_020190.5; NP_064575.1.
DR   UCSC; uc001eer.3; human. [Q9NRN5-1]
DR   AGR; HGNC:24956; -.
DR   CTD; 56944; -.
DR   DisGeNET; 56944; -.
DR   GeneCards; OLFML3; -.
DR   HGNC; HGNC:24956; OLFML3.
DR   HPA; ENSG00000116774; Tissue enhanced (ovary).
DR   MIM; 610088; gene.
DR   neXtProt; NX_Q9NRN5; -.
DR   OpenTargets; ENSG00000116774; -.
DR   PharmGKB; PA134913777; -.
DR   VEuPathDB; HostDB:ENSG00000116774; -.
DR   eggNOG; KOG3545; Eukaryota.
DR   GeneTree; ENSGT00940000158083; -.
DR   InParanoid; Q9NRN5; -.
DR   OMA; QQQFMEY; -.
DR   OrthoDB; 2876896at2759; -.
DR   PhylomeDB; Q9NRN5; -.
DR   TreeFam; TF352000; -.
DR   PathwayCommons; Q9NRN5; -.
DR   SignaLink; Q9NRN5; -.
DR   BioGRID-ORCS; 56944; 243 hits in 1142 CRISPR screens.
DR   ChiTaRS; OLFML3; human.
DR   GeneWiki; OLFML3; -.
DR   GenomeRNAi; 56944; -.
DR   Pharos; Q9NRN5; Tbio.
DR   PRO; PR:Q9NRN5; -.
DR   Proteomes; UP000005640; Chromosome 1.
DR   RNAct; Q9NRN5; Protein.
DR   Bgee; ENSG00000116774; Expressed in gall bladder and 102 other cell types or tissues.
DR   ExpressionAtlas; Q9NRN5; baseline and differential.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:1903561; C:extracellular vesicle; HDA:UniProtKB.
DR   GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR   InterPro; IPR003112; Olfac-like_dom.
DR   PANTHER; PTHR23192:SF8; OLFACTOMEDIN-LIKE PROTEIN 3; 1.
DR   PANTHER; PTHR23192; OLFACTOMEDIN-RELATED; 1.
DR   Pfam; PF02191; OLF; 1.
DR   SMART; SM00284; OLF; 1.
DR   PROSITE; PS51132; OLF; 1.
DR   Genevisible; Q9NRN5; HS.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Coiled coil; Developmental protein; Disulfide bond;
KW   Glycoprotein; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..406
FT                   /note="Olfactomedin-like protein 3"
FT                   /id="PRO_0000020092"
FT   DOMAIN          134..401
FT                   /note="Olfactomedin-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00446"
FT   COILED          25..101
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        177
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        248
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        135..328
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00446"
FT   VAR_SEQ         1..20
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_014128"
FT   VAR_SEQ         21..38
FT                   /note="GQQHHLVEYMERRLAALE -> MAQWHCSQRTQAGGHGVG (in isoform
FT                   2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_014129"
FT   VAR_SEQ         134..287
FT                   /note="DCGYTISQVRSMKILKRFGGPAGLWTKDPLGQTEKIYVLDGTQNDTAFVFPR
FT                   LRDFTLAMAARKASRVRVPFPWVGTGQLVYGGFLYFARRPPGRPGGGGEMENTLQLIKF
FT                   HLANRTVVDSSVFPAEGLIPPYGLTADTYIDLAADEEGLWAVY -> GKQSESRPLTLL
FT                   RTDILPSLLLTLFFTLSQSIVALSSGICFRETSVMPGLLRRGSGDPGRCGTRSFSILPK
FT                   IGGNRWANAHYSPDHPPWGSSCWRHLILASISFSCAFLIRLWLHNLSSEINEDSEAIWW
FT                   PSWSMDQGSTGANREDLRVRWDTE (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:14702039"
FT                   /id="VSP_014130"
FT   VAR_SEQ         288..406
FT                   /note="Missing (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:14702039"
FT                   /id="VSP_014131"
FT   CONFLICT        118
FT                   /note="G -> E (in Ref. 5; BAD96191)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        131
FT                   /note="M -> K (in Ref. 5; BAD96941)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        182
FT                   /note="V -> F (in Ref. 5; BAD96191)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        277
FT                   /note="A -> V (in Ref. 3; AAQ89313)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   406 AA;  46010 MW;  0B5DB1A803BB99D6 CRC64;
     MGPSTPLLIL FLLSWSGPLQ GQQHHLVEYM ERRLAALEER LAQCQDQSSR HAAELRDFKN
     KMLPLLEVAE KEREALRTEA DTISGRVDRL EREVDYLETQ NPALPCVEFD EKVTGGPGTK
     GKGRRNEKYD MVTDCGYTIS QVRSMKILKR FGGPAGLWTK DPLGQTEKIY VLDGTQNDTA
     FVFPRLRDFT LAMAARKASR VRVPFPWVGT GQLVYGGFLY FARRPPGRPG GGGEMENTLQ
     LIKFHLANRT VVDSSVFPAE GLIPPYGLTA DTYIDLAADE EGLWAVYATR EDDRHLCLAK
     LDPQTLDTEQ QWDTPCPREN AEAAFVICGT LYVVYNTRPA SRARIQCSFD ASGTLTPERA
     ALPYFPRRYG AHASLRYNPR ERQLYAWDDG YQIVYKLEMR KKEEEV
//