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In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination (PubMed:20061386)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.","ecNumber":"2.3.2.23","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00388"},{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU10133"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20061386"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00388"}],"value":"Protein modification; protein ubiquitination"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16916645"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17938197"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19111657"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19887602"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21775430"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24389026"}],"value":"Directly interacts with FANCL (PubMed:16916645, PubMed:17938197, PubMed:19111657, PubMed:21775430, PubMed:24389026). 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