{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9N4D9","uniProtkbId":"NTM1_CAEEL","entryAudit":{"firstPublicDate":"2010-10-05","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2001-10-01","entryVersion":137,"sequenceVersion":2},"annotationScore":3.0,"organism":{"scientificName":"Caenorhabditis elegans","taxonId":6239,"lineage":["Eukaryota","Metazoa","Ecdysozoa","Nematoda","Chromadorea","Rhabditida","Rhabditina","Rhabditomorpha","Rhabditoidea","Rhabditidae","Peloderinae","Caenorhabditis"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9BV86"}],"value":"Alpha N-terminal protein methyltransferase 1"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9BV86"}],"value":"2.1.1.244"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9BV86"}],"value":"X-Pro-Lys N-terminal protein methyltransferase 1"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9BV86"}],"value":"NTM1"}]}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000312","source":"WormBase","id":"Y74C9A.3"}],"value":"homt-1"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"WormBase","id":"Y74C9A.3"}],"value":"Y74C9A.3"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9BV86"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32958651"}],"value":"Alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif (By similarity). Probably required for the synthesis of neurotransmitter melatonin from serotonin, which plays a role in promoting a sleep-like state, called lethargus, during larval development (PubMed:32958651)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"N-terminal L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N-terminal N,N,N-trimethyl-L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:54712"},{"database":"Rhea","id":"RHEA-COMP:13785"},{"database":"Rhea","id":"RHEA-COMP:13971"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57856"},{"database":"ChEBI","id":"CHEBI:59789"},{"database":"ChEBI","id":"CHEBI:138057"},{"database":"ChEBI","id":"CHEBI:138315"}],"ecNumber":"2.1.1.244","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9BV86"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"N-terminal L-seryl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N-terminal N,N,N-trimethyl-L-seryl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:54724"},{"database":"Rhea","id":"RHEA-COMP:13789"},{"database":"Rhea","id":"RHEA-COMP:13973"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57856"},{"database":"ChEBI","id":"CHEBI:59789"},{"database":"ChEBI","id":"CHEBI:138061"},{"database":"ChEBI","id":"CHEBI:138317"}],"ecNumber":"2.1.1.244","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9BV86"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"N-terminal L-prolyl-L-prolyl-L-lysyl-[protein] + 2 S-adenosyl-L-methionine = N-terminal N,N-dimethyl-L-prolyl-L-prolyl-L-lysyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:54736"},{"database":"Rhea","id":"RHEA-COMP:13787"},{"database":"Rhea","id":"RHEA-COMP:13974"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57856"},{"database":"ChEBI","id":"CHEBI:59789"},{"database":"ChEBI","id":"CHEBI:138059"},{"database":"ChEBI","id":"CHEBI:138318"}],"ecNumber":"2.1.1.244","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9BV86"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17573073"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32958651"}],"value":"Expressed in uterine cells and PVT neurons of the tail (PubMed:17573073, PubMed:32958651). Expressed in pharynx, intestine and DVB tail neuron (PubMed:32958651)"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32958651"}],"value":"Causes significant increase in synaptic transmission including 55% increase in amplitude of evoked postsynaptic currents and 102% increase in the frequency of miniature postsynaptic currents (PubMed:32958651). Decreases total and motionless sleep due to increase in the frequency of active events during sleep-like state (PubMed:32958651)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the methyltransferase superfamily. NTM1 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":234,"modifier":"EXACT"}},"description":"Alpha N-terminal protein methyltransferase 1","featureId":"PRO_0000399783"},{"type":"Binding site","location":{"start":{"value":71,"modifier":"EXACT"},"end":{"value":71,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59789"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9BV86"}],"ligand":{"name":"S-adenosyl-L-methionine","id":"ChEBI:CHEBI:59789"}},{"type":"Binding site","location":{"start":{"value":76,"modifier":"EXACT"},"end":{"value":76,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59789"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9BV86"}],"ligand":{"name":"S-adenosyl-L-methionine","id":"ChEBI:CHEBI:59789"}},{"type":"Binding site","location":{"start":{"value":93,"modifier":"EXACT"},"end":{"value":95,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59789"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9BV86"}],"ligand":{"name":"S-adenosyl-L-methionine","id":"ChEBI:CHEBI:59789"}},{"type":"Binding site","location":{"start":{"value":120,"modifier":"EXACT"},"end":{"value":121,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59789"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9BV86"}],"ligand":{"name":"S-adenosyl-L-methionine","id":"ChEBI:CHEBI:59789"}},{"type":"Binding site","location":{"start":{"value":136,"modifier":"EXACT"},"end":{"value":136,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59789"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9BV86"}],"ligand":{"name":"S-adenosyl-L-methionine","id":"ChEBI:CHEBI:59789"}}],"keywords":[{"id":"KW-0489","category":"Molecular function","name":"Methyltransferase"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0949","category":"Ligand","name":"S-adenosyl-L-methionine"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"9851916","citationType":"journal article","authoringGroup":["The C. elegans sequencing consortium"],"citationCrossReferences":[{"database":"PubMed","id":"9851916"},{"database":"DOI","id":"10.1126/science.282.5396.2012"}],"title":"Genome sequence of the nematode C. elegans: a platform for investigating biology.","publicationDate":"1998","journal":"Science","firstPage":"2012","lastPage":"2018","volume":"282"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"Bristol N2","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"17573073","citationType":"journal article","authors":["Tanaka D.","Furusawa K.","Kameyama K.","Okamoto H.","Doi M."],"citationCrossReferences":[{"database":"PubMed","id":"17573073"},{"database":"DOI","id":"10.1016/j.neuropharm.2007.04.017"}],"title":"Melatonin signaling regulates locomotion behavior and homeostatic states through distinct receptor pathways in Caenorhabditis elegans.","publicationDate":"2007","journal":"Neuropharmacology","firstPage":"157","lastPage":"168","volume":"53"},"referencePositions":["TISSUE SPECIFICITY"]},{"referenceNumber":3,"citation":{"id":"32958651","citationType":"journal article","authors":["Niu L.","Li Y.","Zong P.","Liu P.","Shui Y.","Chen B.","Wang Z.W."],"citationCrossReferences":[{"database":"PubMed","id":"32958651"},{"database":"DOI","id":"10.1073/pnas.2010928117"}],"title":"Melatonin promotes sleep by activating the BK channel in C. elegans.","publicationDate":"2020","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"25128","lastPage":"25137","volume":"117"},"referencePositions":["FUNCTION","TISSUE SPECIFICITY","DISRUPTION PHENOTYPE"],"evidences":[{"evidenceCode":"ECO:0000305"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"FO080986","properties":[{"key":"ProteinId","value":"CCD68263.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"NP_490660.1","properties":[{"key":"NucleotideSequenceId","value":"NM_058260.8"}]},{"database":"AlphaFoldDB","id":"Q9N4D9","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9N4D9","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"37096","properties":[{"key":"Interactions","value":"3"}]},{"database":"FunCoup","id":"Q9N4D9","properties":[{"key":"Number of interactors","value":"1515"}]},{"database":"STRING","id":"6239.Y74C9A.3.1","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"6239-Y74C9A.3.1","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"Q9N4D9","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblMetazoa","id":"Y74C9A.3.1","properties":[{"key":"ProteinId","value":"Y74C9A.3.1"},{"key":"GeneId","value":"WBGene00022277"}]},{"database":"GeneID","id":"171590","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"cel:CELE_Y74C9A.3","properties":[{"key":"Description","value":"-"}]},{"database":"UCSC","id":"Y74C9A.3.1","properties":[{"key":"OrganismName","value":"c. elegans"}]},{"database":"AGR","id":"WB:WBGene00022277","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"171590","properties":[{"key":"Description","value":"-"}]},{"database":"WormBase","id":"Y74C9A.3","properties":[{"key":"ProteinId","value":"CE28146"},{"key":"GeneId","value":"WBGene00022277"},{"key":"GeneName","value":"homt-1"}]},{"database":"eggNOG","id":"KOG3178","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_055356_3_1_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q9N4D9","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"PVRMYCL","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"1298661at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q9N4D9","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:Q9N4D9","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000001940","properties":[{"key":"Component","value":"Chromosome I"}]},{"database":"Bgee","id":"WBGene00022277","properties":[{"key":"ExpressionPatterns","value":"Expressed in germ line (C elegans) and 4 other cell types or tissues"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0071885","properties":[{"key":"GoTerm","value":"F:N-terminal protein N-methyltransferase activity"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"CDD","id":"cd02440","properties":[{"key":"EntryName","value":"AdoMet_MTases"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.150:FF:000025","properties":[{"key":"EntryName","value":"N-terminal Xaa-Pro-Lys N-methyltransferase 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.150","properties":[{"key":"EntryName","value":"Vaccinia Virus protein VP39"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR008576","properties":[{"key":"EntryName","value":"MeTrfase_NTM1"}]},{"database":"InterPro","id":"IPR029063","properties":[{"key":"EntryName","value":"SAM-dependent_MTases_sf"}]},{"database":"PANTHER","id":"PTHR12753","properties":[{"key":"EntryName","value":"AD-003 - RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12753:SF0","properties":[{"key":"EntryName","value":"ALPHA N-TERMINAL PROTEIN METHYLTRANSFERASE 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF05891","properties":[{"key":"EntryName","value":"Methyltransf_PK"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF016958","properties":[{"key":"EntryName","value":"DUF858_MeTrfase_lik"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53335","properties":[{"key":"EntryName","value":"S-adenosyl-L-methionine-dependent methyltransferases"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSSSSSSRIHNGEDVYEKAEEYWSRASQDVNGMLGGFEALHAPDISASKRFIEGLKKKNLFGYFDYALDCGAGIGRVTKHLLMPFFSKVDMEDVVEELITKSDQYIGKHPRIGDKFVEGLQTFAPPERRYDLIWIQWVSGHLVDEDLVDFFKRCAKGLKPGGCIVLKDNVTNHEKRLFDDDDHSWTRTEPELLKAFADSQLDMVSKALQTGFPKEIYPVKMYALKPQHTGFTNN","length":234,"molWeight":26632,"crc64":"988CDEE1C7CEE4E6","md5":"84434FA21EC0A01FB5D239801CBDB6E2"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":3,"TISSUE SPECIFICITY":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Binding site":5},"uniParcId":"UPI000007A408"}}