{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9LT57","uniProtkbId":"CFM9_ARATH","entryAudit":{"firstPublicDate":"2019-10-16","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2000-10-01","entryVersion":140,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Arabidopsis thaliana","commonName":"Mouse-ear cress","taxonId":3702,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","rosids","malvids","Brassicales","Brassicaceae","Camelineae","Arabidopsis"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"CRM-domain containing factor CFM9, mitochondrial"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"31359042"}],"value":"Protein CRM FAMILY MEMBER 9"}}],"flag":"Precursor"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"31359042"}],"value":"CFM9"},"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"Araport","id":"AT3G27550"}],"value":"At3g27550"}],"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"BAB01285.1"}],"value":"MMJ24.10"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31359042"}],"value":"Involved in the splicing of group II introns in mitochondria (PubMed:31359042). Required for the splicing of mitochondrial introns found in nad1, nad2, nad4, nad5, nad7, rps3 and cox2 genes (PubMed:31359042). Splicing of mitochondrial introns is crucial for mitochondrial biogenesis and function, plant growth and development, and plant response to abiotic stresses (PubMed:31359042)"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31359042"}],"value":"Mitochondrion","id":"SL-0173"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31359042"}],"value":"Highly expressed in roots and meristemic regions of young seedlings (PubMed:31359042). Expressed at low levels in stems, trichomes and stigma (PubMed:31359042)"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31359042"}],"value":"Delayed seed germination, retarded growth and reduced size and height"}],"commentType":"DISRUPTION PHENOTYPE"}],"features":[{"type":"Transit peptide","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":25,"modifier":"EXACT"}},"description":"Mitochondrion","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Chain","location":{"start":{"value":26,"modifier":"EXACT"},"end":{"value":491,"modifier":"EXACT"}},"description":"CRM-domain containing factor CFM9, mitochondrial","featureId":"PRO_0000448354"},{"type":"Domain","location":{"start":{"value":90,"modifier":"EXACT"},"end":{"value":187,"modifier":"EXACT"}},"description":"CRM","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00626"}]},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":34,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":255,"modifier":"EXACT"},"end":{"value":287,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":328,"modifier":"EXACT"},"end":{"value":491,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":22,"modifier":"EXACT"},"end":{"value":34,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":255,"modifier":"EXACT"},"end":{"value":265,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":277,"modifier":"EXACT"},"end":{"value":287,"modifier":"EXACT"}},"description":"Acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":345,"modifier":"EXACT"},"end":{"value":359,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":360,"modifier":"EXACT"},"end":{"value":375,"modifier":"EXACT"}},"description":"Acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":392,"modifier":"EXACT"},"end":{"value":416,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":453,"modifier":"EXACT"},"end":{"value":478,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]}],"keywords":[{"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"id":"KW-0507","category":"Biological process","name":"mRNA processing"},{"id":"KW-0508","category":"Biological process","name":"mRNA splicing"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0687","category":"Molecular function","name":"Ribonucleoprotein"},{"id":"KW-0694","category":"Molecular function","name":"RNA-binding"},{"id":"KW-0809","category":"Domain","name":"Transit peptide"}],"references":[{"referenceNumber":1,"citation":{"id":"10819329","citationType":"journal article","authors":["Sato S.","Nakamura Y.","Kaneko T.","Katoh T.","Asamizu E.","Tabata S."],"citationCrossReferences":[{"database":"PubMed","id":"10819329"},{"database":"DOI","id":"10.1093/dnares/7.2.131"}],"title":"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones.","publicationDate":"2000","journal":"DNA Res.","firstPage":"131","lastPage":"135","volume":"7"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"27862469","citationType":"journal article","authors":["Cheng C.Y.","Krishnakumar V.","Chan A.P.","Thibaud-Nissen F.","Schobel S.","Town C.D."],"citationCrossReferences":[{"database":"PubMed","id":"27862469"},{"database":"DOI","id":"10.1111/tpj.13415"}],"title":"Araport11: a complete reannotation of the Arabidopsis thaliana reference genome.","publicationDate":"2017","journal":"Plant J.","firstPage":"789","lastPage":"804","volume":"89"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"CI-CI4AM1G71UC0S","citationType":"submission","authoringGroup":["The Arabidopsis Information Resource (TAIR)"],"title":"Community-consensus reference genome of Arabidopsis thaliana Col-0.","publicationDate":"FEB-2026","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"31359042","citationType":"journal article","authors":["Lee K.","Park S.J.","Park Y.I.","Kang H."],"citationCrossReferences":[{"database":"PubMed","id":"31359042"},{"database":"DOI","id":"10.1093/pcp/pcz147"}],"title":"CFM9, a mitochondrial CRM protein, is crucial for mitochondrial intron splicing, mitochondria function, and Arabidopsis growth and stress responses.","publicationDate":"2019","journal":"Plant Cell Physiol.","firstPage":"2538","lastPage":"2548","volume":"60"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION","TISSUE SPECIFICITY","DISRUPTION PHENOTYPE"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB025626","properties":[{"key":"ProteinId","value":"BAB01285.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP002686","properties":[{"key":"ProteinId","value":"AEE77336.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP002686","properties":[{"key":"ProteinId","value":"ANM65985.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"OZ408685","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"NP_001319656.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001338891.1"}]},{"database":"RefSeq","id":"NP_189392.1","properties":[{"key":"NucleotideSequenceId","value":"NM_113671.3"}]},{"database":"AlphaFoldDB","id":"Q9LT57","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9LT57","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q9LT57","properties":[{"key":"Number of interactors","value":"691"}]},{"database":"STRING","id":"3702.Q9LT57","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"Q9LT57","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"3702-Q9LT57","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"181725","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"822377","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ath:AT3G27550","properties":[{"key":"Description","value":"-"}]},{"database":"Araport","id":"AT3G27550","properties":[{"key":"Description","value":"-"}]},{"database":"TAIR","id":"AT3G27550","properties":[{"key":"GeneName","value":"CFM9"}]},{"database":"eggNOG","id":"KOG1990","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_032610_0_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q9LT57","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"QKRCFQT","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"PhylomeDB","id":"Q9LT57","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:Q9LT57","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000006548","properties":[{"key":"Component","value":"Chromosome 3"}]},{"database":"ExpressionAtlas","id":"Q9LT57","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0009507","properties":[{"key":"GoTerm","value":"C:chloroplast"},{"key":"GoEvidenceType","value":"ISM:TAIR"}]},{"database":"GO","id":"GO:0005739","properties":[{"key":"GoTerm","value":"C:mitochondrion"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"31359042"}]},{"database":"GO","id":"GO:0003723","properties":[{"key":"GoTerm","value":"F:RNA binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0000373","properties":[{"key":"GoTerm","value":"P:Group II intron splicing"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"31359042"}]},{"database":"GO","id":"GO:0140040","properties":[{"key":"GoTerm","value":"P:mitochondrial polycistronic RNA processing"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"31359042"}]},{"database":"GO","id":"GO:0009737","properties":[{"key":"GoTerm","value":"P:response to abscisic acid"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"31359042"}]},{"database":"GO","id":"GO:0009651","properties":[{"key":"GoTerm","value":"P:response to salt stress"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"31359042"}]},{"database":"GO","id":"GO:0009414","properties":[{"key":"GoTerm","value":"P:response to water deprivation"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"31359042"}]},{"database":"Gene3D","id":"3.30.110.60","properties":[{"key":"EntryName","value":"YhbY-like"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR040286","properties":[{"key":"EntryName","value":"At3g25440-like"}]},{"database":"InterPro","id":"IPR001890","properties":[{"key":"EntryName","value":"RNA-binding_CRM"}]},{"database":"InterPro","id":"IPR035920","properties":[{"key":"EntryName","value":"YhbY-like_sf"}]},{"database":"PANTHER","id":"PTHR31426:SF4","properties":[{"key":"EntryName","value":"CRM-DOMAIN CONTAINING FACTOR CFM9, MITOCHONDRIAL"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR31426","properties":[{"key":"EntryName","value":"GROUP II INTRON SPLICING FACTOR CRS1-LIKE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01985","properties":[{"key":"EntryName","value":"CRS1_YhbY"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM01103","properties":[{"key":"EntryName","value":"CRS1_YhbY"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF75471","properties":[{"key":"EntryName","value":"YhbY-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51295","properties":[{"key":"EntryName","value":"CRM"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MNQVFKGWSRGMSTSRGRSMRSKVESRMRKESGKTLREIRRAKKLKKKLMTDEERLIYNLKRAKKKVALLLQKLKKYDLPELPSPVHDPELFTSEQVQAFKKIGFKNKNYVPVGVRGVFGGVVQNMHMHWKFHETVQVCCDNFPKEKIKEMASMIARLSGGVVINIHNVKTIIMFRGRNYRQPKNLIPVNTLTKRKALFKARFEQALESQKLNIKKTEQQLRRMGVNPEDPVAMASIHRVASTFFNAIDKKEGTPYVFHGDKQSERGTSVDNREESEPGDEDSDQEELDRFIAEIEEAADKEWEEEEAAEQEESGRIRYWNREEFAGRSRTPELRSYGDASHGFRRNDRDTHSQRRPNDSDDDDDDGELDSEDDEIPKRFDRARSSNTNTRRPREDFKRRSPDPRPRPRAQVRSDDDVLSDLDNTMWDSEDEEDAPPANYISSSDDDEDENRTVSASSSKQSRFRNNSSRDGINNSKSKSGKQRDEDWDSD","length":491,"molWeight":57003,"crc64":"39C405151A36DA40","md5":"2AAAFDBEEA88D2C8C03A3544E1657C7A"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"DISRUPTION PHENOTYPE":1},"countByFeatureType":{"Transit peptide":1,"Chain":1,"Domain":1,"Region":3,"Compositional bias":7},"uniParcId":"UPI000009CE3F"}}