{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9LN62","uniProtkbId":"RRA3_ARATH","entryAudit":{"firstPublicDate":"2015-10-14","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2000-10-01","entryVersion":138,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Arabidopsis thaliana","commonName":"Mouse-ear cress","taxonId":3702,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","rosids","malvids","Brassicales","Brassicaceae","Camelineae","Arabidopsis"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Arabinosyltransferase RRA3"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"2.4.2.-"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"21680836"}],"value":"Protein REDUCED RESIDUAL ARABINOSE 3"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"21680836"}],"value":"RRA3"},"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"Araport","id":"AT1G19360"}],"value":"At1g19360"}],"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AAF79453.1"}],"value":"F18O14.8"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21680836"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25944827"}],"value":"Plays a role in the arabinosylation of cell wall components. Involved in the arabinosylation of extensin proteins in root hair cells (PubMed:21680836). Extensins are structural glycoproteins present in cell walls and its arabinosylation is important for root hair cell development and root hair tip growth (PubMed:21680836, PubMed:25944827)"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21680836"}],"value":"Golgi apparatus membrane","id":"SL-0134"},"topology":{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"21680836"}],"value":"Single-pass type II membrane protein","id":"SL-9906"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9JI93"}],"value":"The conserved DXD motif is involved in enzyme activity"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21680836"}],"value":"Reduced root hair length and content of arabinosylated extensins in root cell walls"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the glycosyltransferase 77 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":428,"modifier":"EXACT"}},"description":"Arabinosyltransferase RRA3","featureId":"PRO_0000434539"},{"type":"Topological domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":15,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Transmembrane","location":{"start":{"value":16,"modifier":"EXACT"},"end":{"value":36,"modifier":"EXACT"}},"description":"Helical; Signal-anchor for type II membrane protein","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":37,"modifier":"EXACT"},"end":{"value":428,"modifier":"EXACT"}},"description":"Lumenal","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Motif","location":{"start":{"value":249,"modifier":"EXACT"},"end":{"value":251,"modifier":"EXACT"}},"description":"DXD motif","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Glycosylation","location":{"start":{"value":40,"modifier":"EXACT"},"end":{"value":40,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00498"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":277,"modifier":"EXACT"},"end":{"value":277,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00498"}],"featureId":""}],"keywords":[{"id":"KW-0961","category":"Biological process","name":"Cell wall biogenesis/degradation"},{"id":"KW-0325","category":"PTM","name":"Glycoprotein"},{"id":"KW-0328","category":"Molecular function","name":"Glycosyltransferase"},{"id":"KW-0333","category":"Cellular component","name":"Golgi apparatus"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0735","category":"Domain","name":"Signal-anchor"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"24905498","citationType":"journal article","authors":["Lao J.","Oikawa A.","Bromley J.R.","McInerney P.","Suttangkakul A.","Smith-Moritz A.M.","Plahar H.","Chiu T.-Y.","Gonzalez Fernandez-Nino S.M.G.","Ebert B.","Yang F.","Christiansen K.M.","Hansen S.F.","Stonebloom S.","Adams P.D.","Ronald P.C.","Hillson N.J.","Hadi M.Z.","Vega-Sanchez M.E.","Loque D.","Scheller H.V.","Heazlewood J.L."],"citationCrossReferences":[{"database":"PubMed","id":"24905498"},{"database":"DOI","id":"10.1111/tpj.12577"}],"title":"The plant glycosyltransferase clone collection for functional genomics.","publicationDate":"2014","journal":"Plant J.","firstPage":"517","lastPage":"529","volume":"79"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"11130712","citationType":"journal article","authors":["Theologis A.","Ecker J.R.","Palm C.J.","Federspiel N.A.","Kaul S.","White O.","Alonso J.","Altafi H.","Araujo R.","Bowman C.L.","Brooks S.Y.","Buehler E.","Chan A.","Chao Q.","Chen H.","Cheuk R.F.","Chin C.W.","Chung M.K.","Conn L.","Conway A.B.","Conway A.R.","Creasy T.H.","Dewar K.","Dunn P.","Etgu P.","Feldblyum T.V.","Feng J.-D.","Fong B.","Fujii C.Y.","Gill J.E.","Goldsmith A.D.","Haas B.","Hansen N.F.","Hughes B.","Huizar L.","Hunter J.L.","Jenkins J.","Johnson-Hopson C.","Khan S.","Khaykin E.","Kim C.J.","Koo H.L.","Kremenetskaia I.","Kurtz D.B.","Kwan A.","Lam B.","Langin-Hooper S.","Lee A.","Lee J.M.","Lenz C.A.","Li J.H.","Li Y.-P.","Lin X.","Liu S.X.","Liu Z.A.","Luros J.S.","Maiti R.","Marziali A.","Militscher J.","Miranda M.","Nguyen M.","Nierman W.C.","Osborne B.I.","Pai G.","Peterson J.","Pham P.K.","Rizzo M.","Rooney T.","Rowley D.","Sakano H.","Salzberg S.L.","Schwartz J.R.","Shinn P.","Southwick A.M.","Sun H.","Tallon L.J.","Tambunga G.","Toriumi M.J.","Town C.D.","Utterback T.","Van Aken S.","Vaysberg M.","Vysotskaia V.S.","Walker M.","Wu D.","Yu G.","Fraser C.M.","Venter J.C.","Davis R.W."],"citationCrossReferences":[{"database":"PubMed","id":"11130712"},{"database":"DOI","id":"10.1038/35048500"}],"title":"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.","publicationDate":"2000","journal":"Nature","firstPage":"816","lastPage":"820","volume":"408"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"27862469","citationType":"journal article","authors":["Cheng C.Y.","Krishnakumar V.","Chan A.P.","Thibaud-Nissen F.","Schobel S.","Town C.D."],"citationCrossReferences":[{"database":"PubMed","id":"27862469"},{"database":"DOI","id":"10.1111/tpj.13415"}],"title":"Araport11: a complete reannotation of the Arabidopsis thaliana reference genome.","publicationDate":"2017","journal":"Plant J.","firstPage":"789","lastPage":"804","volume":"89"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"CI-CI4AM1G71UC0S","citationType":"submission","authoringGroup":["The Arabidopsis Information Resource (TAIR)"],"title":"Community-consensus reference genome of Arabidopsis thaliana Col-0.","publicationDate":"FEB-2026","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"CI-3FCPUPDS7DLQA","citationType":"submission","authors":["Shinn P.","Chen H.","Kim C.J.","Quinitio C.","Ecker J.R."],"title":"Arabidopsis ORF clones.","publicationDate":"JUN-2006","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":6,"citation":{"id":"CI-1ND7IVR87IF4I","citationType":"submission","authors":["Totoki Y.","Seki M.","Ishida J.","Nakajima M.","Enju A.","Kamiya A.","Narusaka M.","Shin-i T.","Nakagawa M.","Sakamoto N.","Oishi K.","Kohara Y.","Kobayashi M.","Toyoda A.","Sakaki Y.","Sakurai T.","Iida K.","Akiyama K.","Satou M.","Toyoda T.","Konagaya A.","Carninci P.","Kawai J.","Hayashizaki Y.","Shinozaki K."],"title":"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.","publicationDate":"JUL-2006","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":7,"citation":{"id":"CI-3TH3AFLAVIRO9","citationType":"submission","authors":["Brover V.V.","Troukhan M.E.","Alexandrov N.A.","Lu Y.-P.","Flavell R.B.","Feldmann K.A."],"title":"Full-length cDNA from Arabidopsis thaliana.","publicationDate":"MAR-2002","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"]},{"referenceNumber":8,"citation":{"id":"21680836","citationType":"journal article","authors":["Velasquez S.M.","Ricardi M.M.","Dorosz J.G.","Fernandez P.V.","Nadra A.D.","Pol-Fachin L.","Egelund J.","Gille S.","Harholt J.","Ciancia M.","Verli H.","Pauly M.","Bacic A.","Olsen C.E.","Ulvskov P.","Petersen B.L.","Somerville C.","Iusem N.D.","Estevez J.M."],"citationCrossReferences":[{"database":"PubMed","id":"21680836"},{"database":"DOI","id":"10.1126/science.1206657"}],"title":"O-glycosylated cell wall proteins are essential in root hair growth.","publicationDate":"2011","journal":"Science","firstPage":"1401","lastPage":"1403","volume":"332"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION","DISRUPTION PHENOTYPE"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":9,"citation":{"id":"25944827","citationType":"journal article","authors":["Velasquez S.M.","Marzol E.","Borassi C.","Pol-Fachin L.","Ricardi M.M.","Mangano S.","Juarez S.P.","Salter J.D.","Dorosz J.G.","Marcus S.E.","Knox J.P.","Dinneny J.R.","Iusem N.D.","Verli H.","Estevez J.M."],"citationCrossReferences":[{"database":"PubMed","id":"25944827"},{"database":"DOI","id":"10.1104/pp.114.255521"}],"title":"Low sugar is not always good: impact of specific o-glycan defects on tip growth in Arabidopsis.","publicationDate":"2015","journal":"Plant Physiol.","firstPage":"808","lastPage":"813","volume":"168"},"referencePositions":["FUNCTION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"KJ138999","properties":[{"key":"ProteinId","value":"AHL38939.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AC025808","properties":[{"key":"ProteinId","value":"AAF79453.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP002684","properties":[{"key":"ProteinId","value":"AEE29839.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP002684","properties":[{"key":"ProteinId","value":"ANM59303.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"OZ408683","properties":[{"key":"ProteinId","value":"CAO7027945.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"BT025990","properties":[{"key":"ProteinId","value":"ABG25079.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK229032","properties":[{"key":"ProteinId","value":"BAF00917.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AY088117","properties":[{"key":"ProteinId","value":"AAM65662.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"RefSeq","id":"NP_001321670.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001332391.1"}]},{"database":"RefSeq","id":"NP_564082.1","properties":[{"key":"NucleotideSequenceId","value":"NM_101793.4"}]},{"database":"AlphaFoldDB","id":"Q9LN62","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q9LN62","properties":[{"key":"Number of interactors","value":"1061"}]},{"database":"STRING","id":"3702.Q9LN62","properties":[{"key":"Description","value":"-"}]},{"database":"CAZy","id":"GT77","properties":[{"key":"FamilyName","value":"Glycosyltransferase Family 77"}]},{"database":"GlyCosmos","id":"Q9LN62","properties":[{"key":"glycosylation","value":"2 sites, No reported glycans"}]},{"database":"GlyGen","id":"Q9LN62","properties":[{"key":"glycosylation","value":"2 sites"}]},{"database":"iPTMnet","id":"Q9LN62","properties":[{"key":"Description","value":"-"}]},{"database":"SwissPalm","id":"Q9LN62","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"3702-Q9LN62","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"228200","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"838519","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ath:AT1G19360","properties":[{"key":"Description","value":"-"}]},{"database":"Araport","id":"AT1G19360","properties":[{"key":"Description","value":"-"}]},{"database":"TAIR","id":"AT1G19360","properties":[{"key":"GeneName","value":"RRA3"}]},{"database":"eggNOG","id":"ENOG502QRD4","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_037805_0_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q9LN62","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"DHAHEQF","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"540503at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q9LN62","properties":[{"key":"Description","value":"-"}]},{"database":"CD-CODE","id":"4299E36E","properties":[{"key":"EntryName","value":"Nucleolus"}]},{"database":"PRO","id":"PR:Q9LN62","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000006548","properties":[{"key":"Component","value":"Chromosome 1"}]},{"database":"ExpressionAtlas","id":"Q9LN62","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0005768","properties":[{"key":"GoTerm","value":"C:endosome"},{"key":"GoEvidenceType","value":"HDA:TAIR"}],"evidences":[{"evidenceCode":"ECO:0007005","source":"PubMed","id":"22923678"}]},{"database":"GO","id":"GO:0005794","properties":[{"key":"GoTerm","value":"C:Golgi apparatus"},{"key":"GoEvidenceType","value":"HDA:TAIR"}],"evidences":[{"evidenceCode":"ECO:0007005","source":"PubMed","id":"22430844"},{"evidenceCode":"ECO:0007005","source":"PubMed","id":"22923678"},{"evidenceCode":"ECO:0007005","source":"PubMed","id":"25122472"}]},{"database":"GO","id":"GO:0005797","properties":[{"key":"GoTerm","value":"C:Golgi medial cisterna"},{"key":"GoEvidenceType","value":"IDA:TAIR"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"31266899"}]},{"database":"GO","id":"GO:0000139","properties":[{"key":"GoTerm","value":"C:Golgi membrane"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21680836"}]},{"database":"GO","id":"GO:0005802","properties":[{"key":"GoTerm","value":"C:trans-Golgi network"},{"key":"GoEvidenceType","value":"HDA:TAIR"}],"evidences":[{"evidenceCode":"ECO:0007005","source":"PubMed","id":"22923678"}]},{"database":"GO","id":"GO:0052636","properties":[{"key":"GoTerm","value":"F:arabinosyltransferase activity"},{"key":"GoEvidenceType","value":"IMP:GO_Central"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"21680836"}]},{"database":"GO","id":"GO:0080147","properties":[{"key":"GoTerm","value":"P:root hair cell development"},{"key":"GoEvidenceType","value":"IMP:TAIR"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"21680836"}]},{"database":"InterPro","id":"IPR005069","properties":[{"key":"EntryName","value":"Nucl-diP-sugar_transferase"}]},{"database":"InterPro","id":"IPR029044","properties":[{"key":"EntryName","value":"Nucleotide-diphossugar_trans"}]},{"database":"InterPro","id":"IPR044290","properties":[{"key":"EntryName","value":"RRA1/2/3"}]},{"database":"PANTHER","id":"PTHR46581","properties":[{"key":"EntryName","value":"ARABINOSYLTRANSFERASE RRA3"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46581:SF3","properties":[{"key":"EntryName","value":"ARABINOSYLTRANSFERASE RRA3"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03407","properties":[{"key":"EntryName","value":"Nucleotid_trans"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53448","properties":[{"key":"EntryName","value":"Nucleotide-diphospho-sugar transferases"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAGRRDRSQQLRGSRIAIAILIGIFIGCVCAVLFPYGFFNSSSSLKASEHLSKSSNQVGSSACESPERVKMLKSDFVTLSEKNAELKKQVRELTEKLRLAEQGSDNARKQVLALGTQIKAGPFGTVKSLRTNPTILPDESINPRLAKILEEIAVDKEVIVALANANVKAMLEVQIASIKRVGITNYLVVALDDYIENLCKENDVAYYKRDPDKDVDTVGKTGGNHAVSGLKFRVLREFLQLGYGVLLSDVDIVFLQNPFSHLYRDSDVESMSDGHDNHTAYGFNDVFDEPAMGWARYAHTMRIWVFNSGFFYLRPTIPSIELLDRVADRLSKAKVWDQAVFNEELFYPSHPEYTALHASKRVMDMYEFMNSKVLFKTVRKNHELKKKVKPVIVHVNYHPDKLNRMQAVVEFYVNGKQDALDSFPDGSE","length":428,"molWeight":48251,"crc64":"143DDB43473F0353","md5":"B5DB267102B14CB47E926CFAE1B8D3B0"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"DOMAIN":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Topological domain":2,"Transmembrane":1,"Motif":1,"Glycosylation":2},"uniParcId":"UPI00000A24CF"}}