{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9LM60","uniProtkbId":"B3GT5_ARATH","entryAudit":{"firstPublicDate":"2009-01-20","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2000-10-01","entryVersion":128,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Arabidopsis thaliana","commonName":"Mouse-ear cress","taxonId":3702,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","rosids","malvids","Brassicales","Brassicaceae","Camelineae","Arabidopsis"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"value":"Probable beta-1,3-galactosyltransferase 5"},"ecNumbers":[{"value":"2.4.1.-"}]}},"genes":[{"geneName":{"value":"B3GALT5"},"orderedLocusNames":[{"value":"At1g22015"}],"orfNames":[{"value":"F2E2.6"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal glycosyl residue"}],"commentType":"FUNCTION"},{"commentType":"COFACTOR","cofactors":[{"name":"Mn(2+)","evidences":[{"evidenceCode":"ECO:0000250"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29035"}}]},{"texts":[{"value":"Protein modification; protein glycosylation"}],"commentType":"PATHWAY"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Golgi apparatus membrane","id":"SL-0134"},"topology":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Single-pass type II membrane protein","id":"SL-9906"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the glycosyltransferase 31 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":398,"modifier":"EXACT"}},"description":"Probable beta-1,3-galactosyltransferase 5","featureId":"PRO_0000359415"},{"type":"Transmembrane","location":{"start":{"value":11,"modifier":"EXACT"},"end":{"value":31,"modifier":"EXACT"}},"description":"Helical; Signal-anchor for type II membrane protein","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Glycosylation","location":{"start":{"value":110,"modifier":"EXACT"},"end":{"value":110,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":115,"modifier":"EXACT"},"end":{"value":115,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":206,"modifier":"EXACT"},"end":{"value":206,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""}],"keywords":[{"id":"KW-0325","category":"PTM","name":"Glycoprotein"},{"id":"KW-0328","category":"Molecular function","name":"Glycosyltransferase"},{"id":"KW-0333","category":"Cellular component","name":"Golgi apparatus"},{"id":"KW-0464","category":"Ligand","name":"Manganese"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0735","category":"Domain","name":"Signal-anchor"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"11130712","citationType":"journal article","authors":["Theologis A.","Ecker J.R.","Palm C.J.","Federspiel N.A.","Kaul S.","White O.","Alonso J.","Altafi H.","Araujo R.","Bowman C.L.","Brooks S.Y.","Buehler E.","Chan A.","Chao Q.","Chen H.","Cheuk R.F.","Chin C.W.","Chung M.K.","Conn L.","Conway A.B.","Conway A.R.","Creasy T.H.","Dewar K.","Dunn P.","Etgu P.","Feldblyum T.V.","Feng J.-D.","Fong B.","Fujii C.Y.","Gill J.E.","Goldsmith A.D.","Haas B.","Hansen N.F.","Hughes B.","Huizar L.","Hunter J.L.","Jenkins J.","Johnson-Hopson C.","Khan S.","Khaykin E.","Kim C.J.","Koo H.L.","Kremenetskaia I.","Kurtz D.B.","Kwan A.","Lam B.","Langin-Hooper S.","Lee A.","Lee J.M.","Lenz C.A.","Li J.H.","Li Y.-P.","Lin X.","Liu S.X.","Liu Z.A.","Luros J.S.","Maiti R.","Marziali A.","Militscher J.","Miranda M.","Nguyen M.","Nierman W.C.","Osborne B.I.","Pai G.","Peterson J.","Pham P.K.","Rizzo M.","Rooney T.","Rowley D.","Sakano H.","Salzberg S.L.","Schwartz J.R.","Shinn P.","Southwick A.M.","Sun H.","Tallon L.J.","Tambunga G.","Toriumi M.J.","Town C.D.","Utterback T.","Van Aken S.","Vaysberg M.","Vysotskaia V.S.","Walker M.","Wu D.","Yu G.","Fraser C.M.","Venter J.C.","Davis R.W."],"citationCrossReferences":[{"database":"PubMed","id":"11130712"},{"database":"DOI","id":"10.1038/35048500"}],"title":"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.","publicationDate":"2000","journal":"Nature","firstPage":"816","lastPage":"820","volume":"408"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"27862469","citationType":"journal article","authors":["Cheng C.Y.","Krishnakumar V.","Chan A.P.","Thibaud-Nissen F.","Schobel S.","Town C.D."],"citationCrossReferences":[{"database":"PubMed","id":"27862469"},{"database":"DOI","id":"10.1111/tpj.13415"}],"title":"Araport11: a complete reannotation of the Arabidopsis thaliana reference genome.","publicationDate":"2017","journal":"Plant J.","firstPage":"789","lastPage":"804","volume":"89"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"CI-CI4AM1G71UC0S","citationType":"submission","authoringGroup":["The Arabidopsis Information Resource (TAIR)"],"title":"Community-consensus reference genome of Arabidopsis thaliana Col-0.","publicationDate":"FEB-2026","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"CI-1ND7IVR87IF4I","citationType":"submission","authors":["Totoki Y.","Seki M.","Ishida J.","Nakajima M.","Enju A.","Kamiya A.","Narusaka M.","Shin-i T.","Nakagawa M.","Sakamoto N.","Oishi K.","Kohara Y.","Kobayashi M.","Toyoda A.","Sakaki Y.","Sakurai T.","Iida K.","Akiyama K.","Satou M.","Toyoda T.","Konagaya A.","Carninci P.","Kawai J.","Hayashizaki Y.","Shinozaki K."],"title":"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.","publicationDate":"JUL-2006","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"18548197","citationType":"journal article","authors":["Qu Y.","Egelund J.","Gilson P.R.","Houghton F.","Gleeson P.A.","Schultz C.J.","Bacic A."],"citationCrossReferences":[{"database":"PubMed","id":"18548197"},{"database":"DOI","id":"10.1007/s11103-008-9351-3"}],"title":"Identification of a novel group of putative Arabidopsis thaliana beta-(1,3)-galactosyltransferases.","publicationDate":"2008","journal":"Plant Mol. Biol.","firstPage":"43","lastPage":"59","volume":"68"},"referencePositions":["GENE FAMILY","NOMENCLATURE"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AC069252","properties":[{"key":"ProteinId","value":"AAF86563.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP002684","properties":[{"key":"ProteinId","value":"AEE30185.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"OZ408683","properties":[{"key":"ProteinId","value":"CAO7028260.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AK226416","properties":[{"key":"ProteinId","value":"BAE98561.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"PIR","id":"B86353","properties":[{"key":"EntryName","value":"B86353"}]},{"database":"AlphaFoldDB","id":"Q9LM60","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9LM60","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q9LM60","properties":[{"key":"Number of interactors","value":"1536"}]},{"database":"STRING","id":"3702.Q9LM60","properties":[{"key":"Description","value":"-"}]},{"database":"CAZy","id":"GT31","properties":[{"key":"FamilyName","value":"Glycosyltransferase Family 31"}]},{"database":"GlyCosmos","id":"Q9LM60","properties":[{"key":"glycosylation","value":"3 sites, No reported glycans"}]},{"database":"GlyGen","id":"Q9LM60","properties":[{"key":"glycosylation","value":"3 sites"}]},{"database":"PaxDb","id":"3702-Q9LM60","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"240958","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ath:AT1G22015","properties":[{"key":"Description","value":"-"}]},{"database":"Araport","id":"AT1G22015","properties":[{"key":"Description","value":"-"}]},{"database":"TAIR","id":"AT1G22015","properties":[{"key":"GeneName","value":"DD46"}]},{"database":"eggNOG","id":"KOG2288","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_040730_3_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q9LM60","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"DRMWMVH","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"1158011at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q9LM60","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00378","properties":[{"key":"RectionId","value":"-"}]},{"database":"PRO","id":"PR:Q9LM60","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000006548","properties":[{"key":"Component","value":"Chromosome 1"}]},{"database":"ExpressionAtlas","id":"Q9LM60","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0005794","properties":[{"key":"GoTerm","value":"C:Golgi apparatus"},{"key":"GoEvidenceType","value":"ISM:TAIR"}]},{"database":"GO","id":"GO:0000139","properties":[{"key":"GoTerm","value":"C:Golgi membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008378","properties":[{"key":"GoTerm","value":"F:galactosyltransferase activity"},{"key":"GoEvidenceType","value":"IDA:TAIR"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"34113372"}]},{"database":"GO","id":"GO:0009101","properties":[{"key":"GoTerm","value":"P:glycoprotein biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0010208","properties":[{"key":"GoTerm","value":"P:pollen wall assembly"},{"key":"GoEvidenceType","value":"IGI:TAIR"}],"evidences":[{"evidenceCode":"ECO:0000316","source":"PubMed","id":"35774810"}]},{"database":"FunFam","id":"3.90.550.50:FF:000002","properties":[{"key":"EntryName","value":"Hexosyltransferase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.90.550.50","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR025298","properties":[{"key":"EntryName","value":"DUF4094"}]},{"database":"InterPro","id":"IPR002659","properties":[{"key":"EntryName","value":"Glyco_trans_31"}]},{"database":"PANTHER","id":"PTHR11214:SF242","properties":[{"key":"EntryName","value":"BETA-1,3-GALACTOSYLTRANSFERASE 5-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11214","properties":[{"key":"EntryName","value":"BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF13334","properties":[{"key":"EntryName","value":"DUF4094"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01762","properties":[{"key":"EntryName","value":"Galactosyl_T"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKHNNKVSKRLTMTWVPLLCISCFFLGAIFTSKLRSASSDSGSQLILQHRRDQELKIVTQDYAHEKKKSQDNDVMEEVLKTHKAIESLDKSVSMLQKQLSATHSPQQIVNVSATNSSTEGNQKNKVFMVIGINTAFSSRKRRDSLRETWMPQGEKLEKLEKEKGIVVKFMIGHSSTPNSMLDKEIDSEDAQYNDFFRLDHVEGYYNLSAKTKSFFSSAVAKWDAEFYVKIDDDVHVNLGTLASTLASHRSKPRVYIGCMKSGPVLTKKTAKYREPEFWKFGEEGNKYFRHATGQIYAISKDLATYISNNQPILHKYANEDVTLGSWFIGLEVEQIDDRNFCCGTPPDCEMRAEAGEMCVATFDWKCSGVCRSVDRMWMVHVMCGEGSKAVWDANLKLS","length":398,"molWeight":45201,"crc64":"71FC854998FB968B","md5":"9066846F44CFBF21348F62B43B2E41D1"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"COFACTOR":1,"PATHWAY":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Transmembrane":1,"Glycosylation":3},"uniParcId":"UPI00000A44A6"}}