{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9LCC0","uniProtkbId":"DMFAB_ALCSP","entryAudit":{"firstPublicDate":"2011-01-11","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2000-10-01","entryVersion":66,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Alcaligenes sp","taxonId":512,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Betaproteobacteria","Burkholderiales","Alcaligenaceae","Alcaligenes"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"10664842"},{"evidenceCode":"ECO:0000312","source":"EMBL","id":"BAA90664.1"}],"value":"N,N-dimethylformamidase beta subunit"},"ecNumbers":[{"value":"3.5.1.56"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"Reference","id":"Ref.2"}],"value":"N,N-dimethylformamidase heavy chain"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"BAA90664.1"}],"value":"dmfA2"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10664842"},{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.2"}],"value":"Hydrolyzes N,N-dimethylformamide, and to a lesser extent N,N-dimethylacetamide and N,N-diethylacetamide. Has no activity against the substituted amides N-methylformamide, N-ethylformamide, N-ethylformamide and N-methylacetamide or the unsubstituted amides formamide, nicotinamide, acetoamide, benzamide, acetamide and acrylamide"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"N,N-dimethylformamide + H2O = dimethylamine + formate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:19517"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15740"},{"database":"ChEBI","id":"CHEBI:17741"},{"database":"ChEBI","id":"CHEBI:58040"}],"ecNumber":"3.5.1.56","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10664842"},{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.2"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.2"}],"value":"Activity is slightly inhibited by Mg(2+) and Mn(2+), and slightly increased by Cu(2+). Activity is slightly inhibited by the chelating agents 8-hydroxyquinoline, ethylenediaminetetraacetate, o-phenanthroline and 2,2'-bipyridyl"}],"commentType":"ACTIVITY REGULATION"},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"maximumVelocities":[{"velocity":83.3,"unit":"umol/min/mg","enzyme":"enzyme with N,N-dimethylformamide as substrate","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10664842"},{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.2"}]}],"michaelisConstants":[{"constant":1.28,"unit":"mM","substrate":"N,N-dimethylformamide","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10664842"},{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.2"}]}]},"phDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10664842"},{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.2"}],"value":"Optimum pH is 6.0-7.0."}]},"temperatureDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10664842"},{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.2"}],"value":"Optimum temperature is 50-55 degrees Celsius. Stable up to 50 degrees Celsius, inactivated after incubation at 60 degrees Celsius for 30 minutes. Activity decreases below 50 degrees Celsius, with 20% of activity retained at 25 degrees Celsius."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10664842"},{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.2"}],"value":"Heterotetramer of two DmfA1 (alpha) and two DmfA2 (beta) subunits"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"It is not known which subunit of DmfA1 or DmfA2 possesses catalytic activity"}],"commentType":"CAUTION"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":762,"modifier":"EXACT"}},"description":"N,N-dimethylformamidase beta subunit","featureId":"PRO_0000403316"}],"keywords":[{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"}],"references":[{"referenceNumber":1,"citation":{"id":"10664842","citationType":"journal article","authors":["Hasegawa Y.","Tokuyama T.","Iwaki H."],"citationCrossReferences":[{"database":"PubMed","id":"10664842"},{"database":"DOI","id":"10.1271/bbb.63.2091"}],"title":"Cloning and expression of the N,N-dimethylformamidase gene from Alcaligenes sp. strain KUFA-1.","publicationDate":"1999","journal":"Biosci. Biotechnol. Biochem.","firstPage":"2091","lastPage":"2096","volume":"63"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]","PROTEIN SEQUENCE OF 1-25","FUNCTION","CATALYTIC ACTIVITY","BIOPHYSICOCHEMICAL PROPERTIES","SUBUNIT"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"BAA90664.1"}],"value":"KUFA-1","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000305"},{"evidenceCode":"ECO:0000312","source":"EMBL","id":"BAA90664.1"}]},{"referenceNumber":2,"citation":{"id":"CI-E4SL5PTF2NMH","citationType":"journal article","authors":["Hasegawa Y.","Matsuo M.","Sigemoto Y.","Sakai T.","Tokuyama T."],"title":"Purification and characterization of N,N-dimethylformamidase from Alcaligenes sp. KUFA-1.","publicationDate":"1999","journal":"J. Ferment. 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