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Essential for pSK1 segregational stability (PubMed:12644483). It enhances the separation of plasmids from clusters, driving effective segregation upon cell division (PubMed:35907571). Binds a centromere consisting of seven repeat elements located in the par-rep intergenic region (PubMed:35907571). DNA-binding also mediates autoregulation at the par promoter (Ppar) (PubMed:35907571). It does not influence transcription of the pSK1 replication initiation gene (rep) and plasmid replication (PubMed:12644483)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35907571"}],"value":"Homodimer (PubMed:35907571). Two dimers could interact to form a dimer of dimers (PubMed:35907571)"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35907571"}],"value":"Autoregulated"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35907571"}],"value":"Contains an N-terminal winged helix-turn-helix (wHTH) motif that binds DNA (PubMed:35907571). This DNA-binding region is followed by a flexible linker and a predicted coiled coil region, which mediates Par oligomerization (PubMed:35907571). The C-terminal disordered region is not involved in DNA binding (PubMed:35907571)"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35907571"}],"value":"Plasmids lacking this gene fail to separate into new foci and do not segregate evenly into daughter cells"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12644483"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35907571"}],"value":"Unlike typical partitioning systems, requiring two proteins, Par alone drives pSK1 partition"}],"commentType":"MISCELLANEOUS"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":245,"modifier":"EXACT"}},"description":"Plasmid partition protein Par","featureId":"PRO_0000463939"},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":56,"modifier":"EXACT"}},"description":"Winged helix-turn-helix","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"35907571"}]},{"type":"Region","location":{"start":{"value":57,"modifier":"EXACT"},"end":{"value":78,"modifier":"EXACT"}},"description":"Flexible loop-like region","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"35907571"}]},{"type":"Region","location":{"start":{"value":160,"modifier":"EXACT"},"end":{"value":232,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Coiled coil","location":{"start":{"value":83,"modifier":"EXACT"},"end":{"value":159,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Compositional bias","location":{"start":{"value":178,"modifier":"EXACT"},"end":{"value":189,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":190,"modifier":"EXACT"},"end":{"value":215,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":223,"modifier":"EXACT"},"end":{"value":232,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Mutagenesis","location":{"start":{"value":15,"modifier":"EXACT"},"end":{"value":15,"modifier":"EXACT"}},"description":"Does not bind DNA.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35907571"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":132,"modifier":"EXACT"},"end":{"value":132,"modifier":"EXACT"}},"description":"Shows reduced binding to the centromere.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35907571"}],"alternativeSequence":{"originalSequence":"L","alternativeSequences":["A"]}}],"geneLocations":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AAF63251.1"}],"value":"pSK1","geneEncodingType":"Plasmid"}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0131","category":"Biological process","name":"Cell cycle"},{"id":"KW-0132","category":"Biological process","name":"Cell division"},{"id":"KW-0175","category":"Domain","name":"Coiled coil"},{"id":"KW-0238","category":"Molecular function","name":"DNA-binding"},{"id":"KW-0614","category":"Technical term","name":"Plasmid"},{"id":"KW-0616","category":"Biological process","name":"Plasmid partition"}],"references":[{"referenceNumber":1,"citation":{"id":"12644483","citationType":"journal article","authors":["Simpson A.E.","Skurray R.A.","Firth N."],"citationCrossReferences":[{"database":"PubMed","id":"12644483"},{"database":"DOI","id":"10.1128/jb.185.7.2143-2152.2003"}],"title":"A single gene on the staphylococcal multiresistance plasmid pSK1 encodes a novel partitioning system.","publicationDate":"2003","journal":"J. 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