{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9K0P4","uniProtkbId":"DMP19_NEIMB","entryAudit":{"firstPublicDate":"2025-06-18","lastAnnotationUpdateDate":"2026-01-28","lastSequenceUpdateDate":"2000-10-01","entryVersion":96,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Neisseria meningitidis serogroup B (strain ATCC BAA-335 / MC58)","taxonId":122586,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Betaproteobacteria","Neisseriales","Neisseriaceae","Neisseria"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"29220372"}],"value":"DNA mimic protein DMP19"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"29220372"}],"value":"DNA mimic protein 19 kDa"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"22373915"}],"value":"Neisseria conserved protein DMP19"}}]},"genes":[{"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AAF40970.1"}],"value":"NMB0541"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22373915"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29220372"}],"value":"Acts as a DNA mimic (PubMed:22373915, PubMed:29220372). Interacts with DNA-binding proteins and prevents their binding to DNA by occupying the DNA binding sites on the proteins, acting as a competitive inhibitor (PubMed:29220372). DMP19 is a bifunctional DNA mimic protein involved in controlling nucleoid formation as well as gene regulation (PubMed:22373915, PubMed:29220372). This bifunctionality depends on different oligomeric states (PubMed:29220372). The monomeric form interacts with the DNA-binding protein HU, which prevents HU from binding to DNA and forming nucleoids (PubMed:29220372). The dimeric form interacts with the Neisseria hypothetical transcription factor (NHTF) and prevents NHTF from binding to its DNA-binding sites, thereby blocking its repressor activity and influencing expression of the target genes (PubMed:22373915). DMP19 might use these different oligomerizations to regulate genes in two steps: the monomeric form may first release selected gene regions in chromosomal DNA by preventing HU from binding to DNA and forming nucleoids, then the dimeric form blocks the gene repressor activity of NHTF and ensures the continued expression of NHTF-controlled genes (PubMed:29220372)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25287606"}],"value":"Activity can be modulated in vitro by crown ethers, which are small cyclic polyethers that can modify protein surface behavior dramatically by stabilizing either intra- or intermolecular interactions, thereby probably altering the protein's tertiary and quaternary structure"}],"commentType":"ACTIVITY REGULATION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22373915"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25287606"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29220372"}],"value":"Monomer (PubMed:25287606, PubMed:29220372). Homodimer (PubMed:22373915, PubMed:25287606). The monomeric form of DMP19 interacts with the DNA-binding protein HU homodimer with 1:1 stoichiometry (PubMed:29220372). The dimeric form of DMP19 interacts with the Neisseria hypothetical transcription factor (NHTF) dimer (PubMed:22373915)"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22373915"}],"value":"The dimer exhibits a double-stranded DNA-like negative charge distribution on its surface, which facilitates interaction with DNA-binding proteins"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the DMP19-like protein family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":165,"modifier":"EXACT"}},"description":"DNA mimic protein DMP19","featureId":"PRO_0000462352"},{"type":"Beta 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