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Inhibits TpbB/YfiN allosterically, through a hydrophobic interaction between the C-terminus of YfiR and a conserved region of the periplasmic PAS domain of TpbB/YfiN (PubMed:22719254). Under reducing conditions, may also act as an YfiB-independent sensing device that is able to activate TpbB/YfiN in response to the redox status of the periplasm (PubMed:22719254)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20300602"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22719254"}],"value":"Part of the YfiB-TpbB-YfiR (or yfiBNR) system, encoding a tripartite signaling module that modulates intracellular c-di-GMP levels (PubMed:20300602, PubMed:22719254). The system is a key regulator of the small colony variant (SCV) phenotype, and plays an important role in biofilm formation and in vivo persistence (PubMed:20300602). The c-di-GMP produced by TpbB/YfiN stimulates the production of the Pel and Psl exopolysaccharides, which promotes surface attachment, generates an SCV phenotype and confers resistance against phagocytosis (PubMed:20300602)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22719254"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27113583"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28870806"}],"value":"TpbB/YfiN repression is released through an YfiB-dependent sequestration of YfiR to the outer membrane (PubMed:22719254). Binds vitamin B6 (VB6) or L-Trp at the periphery of the dimer, and both VB6 and L-Trp are able to reduce biofilm formation induced by YfiB L43P mutant (PubMed:27113583). However, VB6 or L-Trp alone may have little effects in interrupting the YfiB-YfiR interaction (PubMed:27113583). GMP enhances the binding affinity between YfiB and YfiR (PubMed:28870806)"}],"commentType":"ACTIVITY REGULATION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22719254"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25849887"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26593397"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27113583"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28870806"}],"value":"Homodimer (PubMed:25849887, PubMed:26593397, PubMed:27113583). Interacts with TpbB/YfiN (PubMed:22719254). Interacts with YfiB (PubMed:26593397, PubMed:27113583, PubMed:28870806). The YfiB-YfiR complex is a 2:2 heterotetramer (PubMed:27113583)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22719254"}],"value":"Shuttles between inner and outer membrane (PubMed:22719254). Localization to the outer membrane fraction is strictly YfiB-dependent (PubMed:22719254)"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20300602"}],"value":"Periplasm","id":"SL-0200"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25849887"}],"value":"Cys-71 and Cys-110 form a disulfide bond in the oxidized form but maintain their free form in the non-oxidized YfiR structure (PubMed:25849887). The Cys-145-Cys-152 disulfide bond is well formed in both structures (PubMed:25849887). The Cys145-Cys152 disulfide bond, but not Cys-71-Cys-110, plays an important role in maintaining the correct folding of the protein (PubMed:25849887)"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20300602"}],"value":"Deletion of the gene results in the generation of a small colony variant (SCV) morphology in PAO1 (PubMed:20300602). Mutant exhibits slow growth, reduced motility, a highly aggregative and wrinkled colony morphology, strong attachment to surfaces and strong, exopolysaccharide-dependent resistance to macrophage phagocytosis (PubMed:20300602). The deletion mutant, although suffering from a tremendous growth disadvantage, shows characteristic persistence behavior under antibiotic selection and during prolonged infection in vivo (PubMed:20300602)"}],"commentType":"DISRUPTION PHENOTYPE"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":190,"modifier":"EXACT"}},"description":"Negative regulator YfiR","featureId":"PRO_0000458198"},{"type":"Binding site","location":{"start":{"value":60,"modifier":"EXACT"},"end":{"value":60,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58115"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28870806"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5Y62"}],"ligand":{"name":"GMP","id":"ChEBI:CHEBI:58115"}},{"type":"Binding 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