{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9I485","uniProtkbId":"C3HPD_PSEAE","entryAudit":{"firstPublicDate":"2023-02-22","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2001-03-01","entryVersion":111,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)","taxonId":208964,"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AAG04648.1"},{"evidenceCode":"ECO:0000312","source":"Proteomes","id":"UP000002438"}],"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Pseudomonadales","Pseudomonadaceae","Pseudomonas"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"27929065"}],"value":"Cis-3-hydroxy-L-proline dehydratase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"c3LHyp dehydratase"},{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"c3LHypD"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27929065"}],"value":"4.2.1.171"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"27929065"}],"value":"Aconitase X"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"27929065"}],"value":"AcnX"}]},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"27929065"}],"value":"PaLhpI"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"27929065"}],"value":"lhpI"},"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"27929065"},{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AAG04648.1"}],"value":"PA1259"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27929065"}],"value":"Catalyzes the dehydration of cis-3-hydroxy-L-proline (c3LHyp) to Delta(1)-pyrroline-2-carboxylate (Pyr2C). Also has activity with (2S,3S,4R)-3,4-dihydroxyproline as substrate, albeit at about 300-fold lower rate. No activity with L-proline, trans-4-hydroxy-L-proline (t4LHyp), cis-4-hydroxy-L-proline (c4LHyp), trans-3-hydroxy-L-proline (t3LHyp), D-proline, cis-4-hydroxy-D-proline (c4DHyp), trans-4-hydroxy-D-proline (t4DHyp) or L-serine as substrates. No hydro-lyase activity with citrate or cis-acotinate. Does not catalyze 2-epimerization of c3LHyp to trans-3-hydroxy-D-proline (t3DHyp). Involved in a degradation pathway that converts c3LHyp to L-proline, which would allow P.aeruginosa to grow on c3LHyp as a sole carbon source"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"cis-3-hydroxy-L-proline = 1-pyrroline-2-carboxylate + H2O","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:47624"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:39785"},{"database":"ChEBI","id":"CHEBI:60041"}],"ecNumber":"4.2.1.171","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27929065"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:47625"},"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27929065"}]}]},{"commentType":"COFACTOR","cofactors":[{"name":"Fe(3+)","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A9CH00"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29034"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27929065"}],"value":"Inhibited by Zn(2+), Cd(2+) and Hg(2+), but not by Co(2+), Ni(2+), Mn(2+), Sr(2+), Mg(2+), or Fe(3+). Inhibited by pyrrole-2-carboxylate and its derivative 2-thiophenecarboxylate, but not by trans-aconitate, fluorocitrate and oxalomalate, which are typical inhibitors of the aconitase enzymes"}],"commentType":"ACTIVITY REGULATION"},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"maximumVelocities":[{"velocity":41.3,"unit":"umol/min/mg","enzyme":"enzyme","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27929065"}]}],"michaelisConstants":[{"constant":0.387,"unit":"mM","substrate":"cis-3-hydroxy-L-proline (at pH 8.0)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27929065"}]}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27929065"}],"value":"kcat is 2820 min(-1). kcat/KM is 7330 min(-1) mM(-1)."}]}},"phDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27929065"}],"value":"Optimum pH is 8-9.5."}]},"temperatureDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27929065"}],"value":"No loss of activity after three months at minus 35 degrees Celsius."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27929065"}],"value":"Monomer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27929065"}],"value":"Expression is induced when the bacterium is grown on trans-4-hydroxy-L-proline (t4LHyp), and to a lesser extent when grown on cis-3-hydroxy-L-proline (c3LHyp) and cis-4-hydroxy-D-proline (c4DHyp) as sole carbon source. Expression is up-regulated by c3LHyp, and by several isomeric 3- and 4-hydroxyprolines"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the AcnX family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":580,"modifier":"EXACT"}},"description":"Cis-3-hydroxy-L-proline dehydratase","featureId":"PRO_0000457275"},{"type":"Active site","location":{"start":{"value":66,"modifier":"EXACT"},"end":{"value":66,"modifier":"EXACT"}},"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"27929065"}]},{"type":"Mutagenesis","location":{"start":{"value":35,"modifier":"EXACT"},"end":{"value":35,"modifier":"EXACT"}},"description":"No effect on catalytic activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27929065"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":66,"modifier":"EXACT"},"end":{"value":66,"modifier":"EXACT"}},"description":"Loss of catalytic activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27929065"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":67,"modifier":"EXACT"},"end":{"value":67,"modifier":"EXACT"}},"description":"Significantly decreased catalytic activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27929065"}],"alternativeSequence":{"originalSequence":"C","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":70,"modifier":"EXACT"},"end":{"value":70,"modifier":"EXACT"}},"description":"Loss of catalytic activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27929065"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":207,"modifier":"EXACT"},"end":{"value":207,"modifier":"EXACT"}},"description":"No effect on catalytic activity. Contains iron.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27929065"}],"alternativeSequence":{"originalSequence":"C","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":294,"modifier":"EXACT"},"end":{"value":294,"modifier":"EXACT"}},"description":"Loss of catalytic activity. Loss of iron.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27929065"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":295,"modifier":"EXACT"},"end":{"value":295,"modifier":"EXACT"}},"description":"Loss of catalytic activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27929065"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":303,"modifier":"EXACT"},"end":{"value":303,"modifier":"EXACT"}},"description":"Loss of catalytic activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27929065"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":309,"modifier":"EXACT"},"end":{"value":309,"modifier":"EXACT"}},"description":"Loss of catalytic activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27929065"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":457,"modifier":"EXACT"},"end":{"value":457,"modifier":"EXACT"}},"description":"About half of the catalytic activity of the wild-type. Contains iron.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27929065"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":459,"modifier":"EXACT"},"end":{"value":459,"modifier":"EXACT"}},"description":"Significantly decreased catalytic activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27929065"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":514,"modifier":"EXACT"},"end":{"value":514,"modifier":"EXACT"}},"description":"Significantly decreased catalytic activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27929065"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":518,"modifier":"EXACT"},"end":{"value":518,"modifier":"EXACT"}},"description":"Loss of catalytic activity. Contains iron.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27929065"}],"alternativeSequence":{"originalSequence":"C","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":536,"modifier":"EXACT"},"end":{"value":536,"modifier":"EXACT"}},"description":"Significantly decreased catalytic activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27929065"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":538,"modifier":"EXACT"},"end":{"value":538,"modifier":"EXACT"}},"description":"Loss of catalytic activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27929065"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["A"]}}],"keywords":[{"id":"KW-0408","category":"Ligand","name":"Iron"},{"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"10984043","citationType":"journal article","authors":["Stover C.K.","Pham X.-Q.T.","Erwin A.L.","Mizoguchi S.D.","Warrener P.","Hickey M.J.","Brinkman F.S.L.","Hufnagle W.O.","Kowalik D.J.","Lagrou M.","Garber R.L.","Goltry L.","Tolentino E.","Westbrock-Wadman S.","Yuan Y.","Brody L.L.","Coulter S.N.","Folger K.R.","Kas A.","Larbig K.","Lim R.M.","Smith K.A.","Spencer D.H.","Wong G.K.-S.","Wu Z.","Paulsen I.T.","Reizer J.","Saier M.H. Jr.","Hancock R.E.W.","Lory S.","Olson M.V."],"citationCrossReferences":[{"database":"PubMed","id":"10984043"},{"database":"DOI","id":"10.1038/35023079"}],"title":"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen.","publicationDate":"2000","journal":"Nature","firstPage":"959","lastPage":"964","volume":"406"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"Proteomes","id":"UP000002438"}],"value":"ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AAG04648.1"},{"evidenceCode":"ECO:0000312","source":"Proteomes","id":"UP000002438"}]},{"referenceNumber":2,"citation":{"id":"27929065","citationType":"journal article","authors":["Watanabe S.","Tajima K.","Fujii S.","Fukumori F.","Hara R.","Fukuda R.","Miyazaki M.","Kino K.","Watanabe Y."],"citationCrossReferences":[{"database":"PubMed","id":"27929065"},{"database":"DOI","id":"10.1038/srep38720"}],"title":"Functional characterization of aconitase X as a cis-3-hydroxy-L-proline dehydratase.","publicationDate":"2016","journal":"Sci. Rep.","firstPage":"38720","lastPage":"38720","volume":"6"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY","SUBSTRATE SPECIFICITY","ACTIVITY REGULATION","BIOPHYSICOCHEMICAL PROPERTIES","SUBUNIT","INDUCTION","ACTIVE SITE","IRON-BINDING","MUTAGENESIS OF ASP-35; SER-66; CYS-67; SER-70; CYS-207; GLU-294; SER-295; SER-303; THR-309; ASN-457; HIS-459; ASP-514; CYS-518; SER-536 AND LYS-538"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"27929065"}],"value":"ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AE004091","properties":[{"key":"ProteinId","value":"AAG04648.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"A83489","properties":[{"key":"EntryName","value":"A83489"}]},{"database":"RefSeq","id":"NP_249950.1","properties":[{"key":"NucleotideSequenceId","value":"NC_002516.2"}]},{"database":"RefSeq","id":"WP_003114962.1","properties":[{"key":"NucleotideSequenceId","value":"NC_002516.2"}]},{"database":"AlphaFoldDB","id":"Q9I485","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9I485","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"208964.PA1259","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"208964-PA1259","properties":[{"key":"Description","value":"-"}]},{"database":"PRIDE","id":"Q9I485","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"881330","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"pae:PA1259","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|208964.12.peg.1307","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"PseudoCAP","id":"PA1259","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_018825_2_0_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q9I485","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"1550274at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q9I485","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"PAER208964:G1FZ6-1284-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"4.2.1.171","properties":[{"key":"OrganismId","value":"5087"}]},{"database":"Proteomes","id":"UP000002438","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0019470","properties":[{"key":"GoTerm","value":"P:trans-4-hydroxy-L-proline catabolic process"},{"key":"GoEvidenceType","value":"IMP:PseudoCAP"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"27145750"}]},{"database":"CDD","id":"cd01355","properties":[{"key":"EntryName","value":"AcnX"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd01356","properties":[{"key":"EntryName","value":"AcnX_swivel"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.499.10","properties":[{"key":"EntryName","value":"Aconitase, domain 3"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.50.30.10","properties":[{"key":"EntryName","value":"Phosphohistidine domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR015931","properties":[{"key":"EntryName","value":"Acnase/IPM_dHydase_lsu_aba_1/3"}]},{"database":"InterPro","id":"IPR012047","properties":[{"key":"EntryName","value":"AcnX"}]},{"database":"InterPro","id":"IPR060004","properties":[{"key":"EntryName","value":"AcnX_1st"}]},{"database":"InterPro","id":"IPR060005","properties":[{"key":"EntryName","value":"AcnX_2nd"}]},{"database":"InterPro","id":"IPR060006","properties":[{"key":"EntryName","value":"AcnX_3rd"}]},{"database":"InterPro","id":"IPR036008","properties":[{"key":"EntryName","value":"Aconitase_4Fe-4S_dom"}]},{"database":"InterPro","id":"IPR007506","properties":[{"key":"EntryName","value":"PMDh-L-like_dom"}]},{"database":"InterPro","id":"IPR002840","properties":[{"key":"EntryName","value":"PMDh-S-like_dom"}]},{"database":"NCBIfam","id":"NF051439","properties":[{"key":"EntryName","value":"C3LHypDHLhpIPSEAE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR36577","properties":[{"key":"EntryName","value":"DUF521 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G00490)"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR36577:SF3","properties":[{"key":"EntryName","value":"DUF521 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G00490)"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04412","properties":[{"key":"EntryName","value":"AcnX"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF28403","properties":[{"key":"EntryName","value":"AcnX_2nd"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF28406","properties":[{"key":"EntryName","value":"AcnX_3rd"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01989","properties":[{"key":"EntryName","value":"AcnX_swivel_put"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF036630","properties":[{"key":"EntryName","value":"UCP036630"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53732","properties":[{"key":"EntryName","value":"Aconitase iron-sulfur domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52016","properties":[{"key":"EntryName","value":"LeuD/IlvD-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKHAHLIVPRTLVAGSASGELLYAPTGLSFWGGVDPRSAEVIDRHHPLSGRHLHGRLLAIPGGRGSCTGSSVLLELILGGRAPAAILLREPDEILALGAIVAEELFGRSLPIACLGERFDELAAYPWARLADGRLELHRDAPPPLEARPAEALATDAGPRLDAFDQALLAGEHGEAARLAMRIVLRMAALQGAQRLIDIQRAHIDACIYTGPAGLRFAETLRDLGARVRVPTTLNAISVDQRRWREQGVPAALGEPAAALARAYLDMGAQPSFTCAPYLLDDSARAGEQIVWAESNAVLFANSVLGARTNKYADFMDICCALTGRAPLAGCHLDEQRQARVLIEVEDLGSVDDAFYPTLGYLCGLLCDGQIPAIDGLRQRQPDHDALKAFGAALGTSSSVPMFHVIGVTPEAPDLASAFGGRAPRRTLRVGRERLRDAWRELDSAGETRIDLVALGNPHFSASEFAQLAALCHGRRRHPEVALVITSSRQVVAQAEAAGHLATLQAFGARLVTDTCWCMLDEPLVPPGARTLMTNSAKYAHYAPGLVGRQVRFAGLAGCVEAAVGGRSPAGLPAWLSEDC","length":580,"molWeight":61813,"crc64":"88619DC0D4611B5C","md5":"8AB04F1982C34FF48C95B2C7243802D8"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"ACTIVITY REGULATION":1,"BIOPHYSICOCHEMICAL PROPERTIES":1,"SUBUNIT":1,"INDUCTION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Active site":1,"Mutagenesis":15},"uniParcId":"UPI00000C52D5"}}