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May transport phospholipids between the inner membrane and the outer membrane. Important for proper cell morphology and integrity of the outer membrane"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38305166"}],"value":"EpaF, TamB, YdbH and YhdP are redundant, but not equivalent, in performing an essential function for growth and maintaining lipid homeostasis in the outer membrane. Any of these four proteins is sufficient for growth"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"38305166"}],"value":"Cell inner membrane","id":"SL-0037"},"topology":{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"38305166"}],"value":"Single-pass type II membrane protein","id":"SL-9906"},"orientation":{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"38305166"}],"value":"Periplasmic side","id":"SL-9916"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38305166"}],"value":"The single mutant does not exhibit growth defects or cellular morphology alterations. The tamB-epaF double mutant shows further increase in outer membrane (OM) permeability to fluorescent probe 1-N-phenylnaphthylamine (NPN) than single tamB mutant. The defects in the OM permeability are partially rescued by lowering the synthesis or transport of lipopolysaccharide (LPS). The tamB-epaF-yhdP triple mutant has reduced growth yield in a planktonic growth assay performed in Mueller-Hinton (MH) broth. The cells of the tamB-epaF-yhdP triple mutant are smaller and rounder than wild-type. Z-ring assembly or localization is not affected in the double or triple mutants. 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