{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9HUX1","uniProtkbId":"SPEA_PSEAE","entryAudit":{"firstPublicDate":"2004-03-29","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2001-03-01","entryVersion":123,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)","taxonId":208964,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Pseudomonadales","Pseudomonadaceae","Pseudomonas"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01417"}],"value":"Biosynthetic arginine decarboxylase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01417"}],"value":"ADC"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01417"}],"value":"4.1.1.19"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01417"}],"value":"speA"},"orderedLocusNames":[{"value":"PA4839"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01417"}],"value":"Catalyzes the biosynthesis of agmatine from arginine"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"L-arginine + H(+) = agmatine + CO2","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:17641"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16526"},{"database":"ChEBI","id":"CHEBI:32682"},{"database":"ChEBI","id":"CHEBI:58145"}],"ecNumber":"4.1.1.19","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01417"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01417"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"commentType":"COFACTOR","cofactors":[{"name":"pyridoxal 5'-phosphate","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01417"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:597326"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01417"}],"value":"Belongs to the Orn/Lys/Arg decarboxylase class-II family. SpeA subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":636,"modifier":"EXACT"}},"description":"Biosynthetic arginine decarboxylase","featureId":"PRO_0000149971"},{"type":"Binding site","location":{"start":{"value":290,"modifier":"EXACT"},"end":{"value":300,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01417"}],"ligand":{"name":"substrate"}},{"type":"Modified residue","location":{"start":{"value":110,"modifier":"EXACT"},"end":{"value":110,"modifier":"EXACT"}},"description":"N6-(pyridoxal phosphate)lysine","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01417"}]}],"keywords":[{"id":"KW-0210","category":"Molecular function","name":"Decarboxylase"},{"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0620","category":"Biological process","name":"Polyamine biosynthesis"},{"id":"KW-0663","category":"Ligand","name":"Pyridoxal phosphate"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0745","category":"Biological process","name":"Spermidine biosynthesis"}],"references":[{"referenceNumber":1,"citation":{"id":"10984043","citationType":"journal article","authors":["Stover C.K.","Pham X.-Q.T.","Erwin A.L.","Mizoguchi S.D.","Warrener P.","Hickey M.J.","Brinkman F.S.L.","Hufnagle W.O.","Kowalik D.J.","Lagrou M.","Garber R.L.","Goltry L.","Tolentino E.","Westbrock-Wadman S.","Yuan Y.","Brody L.L.","Coulter S.N.","Folger K.R.","Kas A.","Larbig K.","Lim R.M.","Smith K.A.","Spencer D.H.","Wong G.K.-S.","Wu Z.","Paulsen I.T.","Reizer J.","Saier M.H. Jr.","Hancock R.E.W.","Lory S.","Olson M.V."],"citationCrossReferences":[{"database":"PubMed","id":"10984043"},{"database":"DOI","id":"10.1038/35023079"}],"title":"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen.","publicationDate":"2000","journal":"Nature","firstPage":"959","lastPage":"964","volume":"406"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AE004091","properties":[{"key":"ProteinId","value":"AAG08224.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"H83040","properties":[{"key":"EntryName","value":"H83040"}]},{"database":"RefSeq","id":"NP_253526.1","properties":[{"key":"NucleotideSequenceId","value":"NC_002516.2"}]},{"database":"RefSeq","id":"WP_003095365.1","properties":[{"key":"NucleotideSequenceId","value":"NC_002516.2"}]},{"database":"AlphaFoldDB","id":"Q9HUX1","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9HUX1","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q9HUX1","properties":[{"key":"Number of interactors","value":"169"}]},{"database":"STRING","id":"208964.PA4839","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"208964-PA4839","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"879594","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"pae:PA4839","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|208964.12.peg.5070","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"PseudoCAP","id":"PA4839","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_027243_1_0_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q9HUX1","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"9802658at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q9HUX1","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:MONOMER-12","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"PAER208964:G1FZ6-4953-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"4.1.1.19","properties":[{"key":"OrganismId","value":"5087"}]},{"database":"Proteomes","id":"UP000002438","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"Q9HUX1","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0008792","properties":[{"key":"GoTerm","value":"F:arginine decarboxylase activity"},{"key":"GoEvidenceType","value":"IMP:CACAO"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"12634339"}]},{"database":"GO","id":"GO:0006527","properties":[{"key":"GoTerm","value":"P:L-arginine catabolic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0008295","properties":[{"key":"GoTerm","value":"P:spermidine biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd06830","properties":[{"key":"EntryName","value":"PLPDE_III_ADC"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.20.58.930:FF:000005","properties":[{"key":"EntryName","value":"Biosynthetic arginine decarboxylase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.20.20.10:FF:000001","properties":[{"key":"EntryName","value":"Biosynthetic arginine decarboxylase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.287.3440","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.20.58.930","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.20.20.10","properties":[{"key":"EntryName","value":"Alanine racemase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.37.10","properties":[{"key":"EntryName","value":"Lyase, Ornithine Decarboxylase, Chain A, domain 1"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01417","properties":[{"key":"EntryName","value":"SpeA"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR009006","properties":[{"key":"EntryName","value":"Ala_racemase/Decarboxylase_C"}]},{"database":"InterPro","id":"IPR040634","properties":[{"key":"EntryName","value":"Arg_decarb_HB"}]},{"database":"InterPro","id":"IPR041128","properties":[{"key":"EntryName","value":"Arg_decarbox_C"}]},{"database":"InterPro","id":"IPR002985","properties":[{"key":"EntryName","value":"Arg_decrbxlase"}]},{"database":"InterPro","id":"IPR022657","properties":[{"key":"EntryName","value":"De-COase2_CS"}]},{"database":"InterPro","id":"IPR022644","properties":[{"key":"EntryName","value":"De-COase2_N"}]},{"database":"InterPro","id":"IPR022653","properties":[{"key":"EntryName","value":"De-COase2_pyr-phos_BS"}]},{"database":"InterPro","id":"IPR000183","properties":[{"key":"EntryName","value":"Orn/DAP/Arg_de-COase"}]},{"database":"InterPro","id":"IPR029066","properties":[{"key":"EntryName","value":"PLP-binding_barrel"}]},{"database":"NCBIfam","id":"NF003763","properties":[{"key":"EntryName","value":"PRK05354.1"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR01273","properties":[{"key":"EntryName","value":"speA"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43295","properties":[{"key":"EntryName","value":"ARGININE DECARBOXYLASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43295:SF9","properties":[{"key":"EntryName","value":"BIOSYNTHETIC ARGININE DECARBOXYLASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF17810","properties":[{"key":"EntryName","value":"Arg_decarb_HB"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF17944","properties":[{"key":"EntryName","value":"Arg_decarbox_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02784","properties":[{"key":"EntryName","value":"Orn_Arg_deC_N"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF001336","properties":[{"key":"EntryName","value":"Arg_decrbxlase"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR01180","properties":[{"key":"EntryName","value":"ARGDCRBXLASE"}]},{"database":"PRINTS","id":"PR01179","properties":[{"key":"EntryName","value":"ODADCRBXLASE"}]},{"database":"SUPFAM","id":"SSF50621","properties":[{"key":"EntryName","value":"Alanine racemase C-terminal domain-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51419","properties":[{"key":"EntryName","value":"PLP-binding barrel"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00878","properties":[{"key":"EntryName","value":"ODR_DC_2_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00879","properties":[{"key":"EntryName","value":"ODR_DC_2_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAARRTRKDDGSNWTVADSRGVYGIRHWGAGYFAINDGGNVEVRPQGADSTPIDLYELVGQLREAGLSLPLLVRFPDILQDRVRKLTGAFDANIERLEYQSRYTALYPIKVNQQEAVVENIIATENVSIGLEAGSKPELMAVLALAPKGGTIVCNGYKDREFIKLALMGQKLGHNVFIVIEKESEVQLVIEEAANVGVQPQVGLRVRLSSLASSKWADTGGEKAKFGLSAAQLLSVVERFRQAGLDQGVRLLHFHMGSQIANLADYQHGFKEAIRYYGELRALGLPVDHIDVGGGLGVDYDGTHSRNASSINYDIDDYAGVVVGMLKEFCDAQGLPHPHIFSESGRALTAHHAVLITQVTDVERHNDDVPKIVDLDEQPEIVRWLAELLGPTDAEMVTETYWRATHYIGDAAAQYADGKISLAQKALAEQCYFAICRRLHNQLKARQRSHRQVLDELNDKLADKYICNFSVFQSLPDTWAIGQVLPILPLHRLGEEPDRRAVLQDLTCDSDGKITQYVDEQSIETSLPVHEVKEGEDYLIGVFLVGAYQEILGDMHNLFGDTDSVNVYQRADGGIYHAGIETHDTIEDMLRYVHLSPEELMTLYRDKVAGAKLTARERNQYLDALRLGLTRSAYLS","length":636,"molWeight":70668,"crc64":"E7274FD432E0D187","md5":"2B3B664B3DDBAA450286E18AB7433D74"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":2,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Binding site":1,"Modified residue":1},"uniParcId":"UPI00000C5E34"}}