{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9FN15","uniProtkbId":"GLD27_ARATH","entryAudit":{"firstPublicDate":"2017-10-25","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2001-03-01","entryVersion":134,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Arabidopsis thaliana","commonName":"Mouse-ear cress","taxonId":3702,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","rosids","malvids","Brassicales","Brassicaceae","Camelineae","Arabidopsis"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"23043051"}],"value":"Protein CONSERVED IN THE GREEN LINEAGE AND DIATOMS 27, chloroplastic"}},"flag":"Precursor"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"23043051"}],"value":"CGLD27"},"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"Araport","id":"AT5G67370"}],"value":"At5g67370"}],"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"BAB09022.1"}],"value":"K8K14.9"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23043051"}],"value":"Required for growth in low iron conditions"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255"}],"value":"Membrane","id":"SL-0162"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255"}],"value":"Multi-pass membrane protein","id":"SL-9909"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000255"}],"value":"Plastid, chloroplast","id":"SL-0049"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23043051"}],"value":"Mostly expressed in seeds, leaves and flowers, and, to a lower extent, in roots"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23043051"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23888164"}],"value":"Accumulates progressively upon iron deficiency"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23043051"}],"value":"Stronger growth defect in iron-deficient conditions"}],"commentType":"DISRUPTION PHENOTYPE"}],"features":[{"type":"Transit peptide","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":59,"modifier":"EXACT"}},"description":"Chloroplast","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Chain","location":{"start":{"value":60,"modifier":"EXACT"},"end":{"value":327,"modifier":"EXACT"}},"description":"Protein CONSERVED IN THE GREEN LINEAGE AND DIATOMS 27, chloroplastic","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":"PRO_0000442037"},{"type":"Transmembrane","location":{"start":{"value":119,"modifier":"EXACT"},"end":{"value":139,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":148,"modifier":"EXACT"},"end":{"value":168,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":225,"modifier":"EXACT"},"end":{"value":245,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Region","location":{"start":{"value":66,"modifier":"EXACT"},"end":{"value":88,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]}],"keywords":[{"id":"KW-0150","category":"Cellular component","name":"Chloroplast"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0934","category":"Cellular component","name":"Plastid"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0809","category":"Domain","name":"Transit peptide"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"9501997","citationType":"journal article","authors":["Nakamura Y.","Sato S.","Kaneko T.","Kotani H.","Asamizu E.","Miyajima N.","Tabata S."],"citationCrossReferences":[{"database":"PubMed","id":"9501997"},{"database":"DOI","id":"10.1093/dnares/4.6.401"}],"title":"Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones.","publicationDate":"1997","journal":"DNA Res.","firstPage":"401","lastPage":"414","volume":"4"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"27862469","citationType":"journal article","authors":["Cheng C.Y.","Krishnakumar V.","Chan A.P.","Thibaud-Nissen F.","Schobel S.","Town C.D."],"citationCrossReferences":[{"database":"PubMed","id":"27862469"},{"database":"DOI","id":"10.1111/tpj.13415"}],"title":"Araport11: a complete reannotation of the Arabidopsis thaliana reference genome.","publicationDate":"2017","journal":"Plant J.","firstPage":"789","lastPage":"804","volume":"89"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"CI-CI4AM1G71UC0S","citationType":"submission","authoringGroup":["The Arabidopsis Information Resource (TAIR)"],"title":"Community-consensus reference genome of Arabidopsis thaliana Col-0.","publicationDate":"FEB-2026","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"14593172","citationType":"journal article","authors":["Yamada K.","Lim J.","Dale J.M.","Chen H.","Shinn P.","Palm C.J.","Southwick A.M.","Wu H.C.","Kim C.J.","Nguyen M.","Pham P.K.","Cheuk R.F.","Karlin-Newmann G.","Liu S.X.","Lam B.","Sakano H.","Wu T.","Yu G.","Miranda M.","Quach H.L.","Tripp M.","Chang C.H.","Lee J.M.","Toriumi M.J.","Chan M.M.","Tang C.C.","Onodera C.S.","Deng J.M.","Akiyama K.","Ansari Y.","Arakawa T.","Banh J.","Banno F.","Bowser L.","Brooks S.Y.","Carninci P.","Chao Q.","Choy N.","Enju A.","Goldsmith A.D.","Gurjal M.","Hansen N.F.","Hayashizaki Y.","Johnson-Hopson C.","Hsuan V.W.","Iida K.","Karnes M.","Khan S.","Koesema E.","Ishida J.","Jiang P.X.","Jones T.","Kawai J.","Kamiya A.","Meyers C.","Nakajima M.","Narusaka M.","Seki M.","Sakurai T.","Satou M.","Tamse R.","Vaysberg M.","Wallender E.K.","Wong C.","Yamamura Y.","Yuan S.","Shinozaki K.","Davis R.W.","Theologis A.","Ecker J.R."],"citationCrossReferences":[{"database":"PubMed","id":"14593172"},{"database":"DOI","id":"10.1126/science.1088305"}],"title":"Empirical analysis of transcriptional activity in the Arabidopsis genome.","publicationDate":"2003","journal":"Science","firstPage":"842","lastPage":"846","volume":"302"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"23043051","citationType":"journal article","authors":["Urzica E.I.","Casero D.","Yamasaki H.","Hsieh S.I.","Adler L.N.","Karpowicz S.J.","Blaby-Haas C.E.","Clarke S.G.","Loo J.A.","Pellegrini M.","Merchant S.S."],"citationCrossReferences":[{"database":"PubMed","id":"23043051"},{"database":"DOI","id":"10.1105/tpc.112.102491"}],"title":"Systems and trans-system level analysis identifies conserved iron deficiency responses in the plant lineage.","publicationDate":"2012","journal":"Plant Cell","firstPage":"3921","lastPage":"3948","volume":"24"},"referencePositions":["FUNCTION","DISRUPTION PHENOTYPE","TISSUE SPECIFICITY","INDUCTION BY IRON DEFICIENCY"],"referenceComments":[{"value":"cv. Col-4","type":"STRAIN"}]},{"referenceNumber":6,"citation":{"id":"23888164","citationType":"journal article","authors":["Rodriguez-Celma J.","Pan I.C.","Li W.","Lan P.","Buckhout T.J.","Schmidt W."],"citationCrossReferences":[{"database":"PubMed","id":"23888164"},{"database":"DOI","id":"10.3389/fpls.2013.00276"}],"title":"The transcriptional response of Arabidopsis leaves to Fe deficiency.","publicationDate":"2013","journal":"Front. Plant Sci.","firstPage":"276","lastPage":"276","volume":"4"},"referencePositions":["INDUCTION BY IRON DEFICIENCY"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB007645","properties":[{"key":"ProteinId","value":"BAB09022.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP002688","properties":[{"key":"ProteinId","value":"AED98333.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"OZ408687","properties":[{"key":"ProteinId","value":"CAO7058150.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AF360127","properties":[{"key":"ProteinId","value":"AAK25837.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AY051028","properties":[{"key":"ProteinId","value":"AAK93705.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"RefSeq","id":"NP_201538.1","properties":[{"key":"NucleotideSequenceId","value":"NM_126137.4"}]},{"database":"AlphaFoldDB","id":"Q9FN15","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q9FN15","properties":[{"key":"Number of interactors","value":"472"}]},{"database":"STRING","id":"3702.Q9FN15","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"3702-Q9FN15","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"247254","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"836872","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ath:AT5G67370","properties":[{"key":"Description","value":"-"}]},{"database":"Araport","id":"AT5G67370","properties":[{"key":"Description","value":"-"}]},{"database":"TAIR","id":"AT5G67370","properties":[{"key":"GeneName","value":"CGLD27"}]},{"database":"eggNOG","id":"ENOG502QS25","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_071172_0_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q9FN15","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"TWIEEGY","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"PhylomeDB","id":"Q9FN15","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:Q9FN15","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000006548","properties":[{"key":"Component","value":"Chromosome 5"}]},{"database":"GO","id":"GO:0009507","properties":[{"key":"GoTerm","value":"C:chloroplast"},{"key":"GoEvidenceType","value":"ISM:TAIR"}]},{"database":"GO","id":"GO:0016020","properties":[{"key":"GoTerm","value":"C:membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:1990641","properties":[{"key":"GoTerm","value":"P:response to iron ion starvation"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"23043051"}]},{"database":"InterPro","id":"IPR009631","properties":[{"key":"EntryName","value":"CGLD27-like"}]},{"database":"PANTHER","id":"PTHR34214","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR34214:SF3","properties":[{"key":"EntryName","value":"PROTEIN CONSERVED IN THE GREEN LINEAGE AND DIATOMS 27, CHLOROPLASTIC"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF06799","properties":[{"key":"EntryName","value":"CGLD27-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MLRLIVNYPLIPKISHRVCSNSSSKLGSYYDSSSIIKYGGISDVVGKKQELFLSVSVKAVEDKGNNGGGSMSFSGQSWDPSSEIEVPSDQRPVNEYSSLKEGMLYSWGELGPSEFFIRLGGLWLVTFTVLGVPVAAASFNPSREPLRFILAAGTGTLFLVSLIVLRIYLGWSYVGDRLLSAVIPYEESGWYDGQMWVKPPEVLARDRLLGSYKVKPVIKMLKQTLIGTGALLVSAFVLFVFATPVEDFFKTTLGSTENQPEVSISRTSNKFNIRKEQLLRLPVDVVTDDDLAAAAAEAADGRPVYCRDRYYRALAGGQYCKWEDLVK","length":327,"molWeight":36114,"crc64":"1CEC05AA8A7EB15B","md5":"5EBDE9343A2E4CFC27420991EAC2E83C"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"INDUCTION":1,"DISRUPTION PHENOTYPE":1},"countByFeatureType":{"Transit peptide":1,"Chain":1,"Transmembrane":3,"Region":1},"uniParcId":"UPI00000AA4CB"}}