{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9FFZ7","uniProtkbId":"CASP4_ARATH","entryAudit":{"firstPublicDate":"2007-10-23","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2001-03-01","entryVersion":119,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Arabidopsis thaliana","commonName":"Mouse-ear cress","taxonId":3702,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","rosids","malvids","Brassicales","Brassicaceae","Camelineae","Arabidopsis"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Casparian strip membrane protein 4"},"shortNames":[{"value":"AtCASP4"}]}},"genes":[{"geneName":{"value":"CASP4"},"orderedLocusNames":[{"value":"At5g06200"}],"orfNames":[{"value":"MBL20.8"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21593871"}],"value":"Regulates membrane-cell wall junctions and localized cell wall deposition. Required for establishment of the Casparian strip membrane domain (CSD) and the subsequent formation of Casparian strips, a cell wall modification of the root endodermis that determines an apoplastic barrier between the intraorganismal apoplasm and the extraorganismal apoplasm and prevents lateral diffusion"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21593871"}],"value":"Homodimer and heterodimers with other CASP proteins. Interacts with CASP1, CASP2, CASP3 and CASP5"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"value":"Very restricted localization following a belt shape within the plasma membrane which coincides with the position of the Casparian strip membrane domain"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21593871"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21593871"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the Casparian strip membrane proteins (CASP) family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":202,"modifier":"EXACT"}},"description":"Casparian strip membrane protein 4","featureId":"PRO_0000308684"},{"type":"Topological domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":40,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":41,"modifier":"EXACT"},"end":{"value":61,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":62,"modifier":"EXACT"},"end":{"value":90,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":91,"modifier":"EXACT"},"end":{"value":111,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":112,"modifier":"EXACT"},"end":{"value":130,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":131,"modifier":"EXACT"},"end":{"value":151,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":152,"modifier":"EXACT"},"end":{"value":176,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":177,"modifier":"EXACT"},"end":{"value":197,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":198,"modifier":"EXACT"},"end":{"value":202,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]}],"keywords":[{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0961","category":"Biological process","name":"Cell wall biogenesis/degradation"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-3E0PO5DBFHD57","citationType":"submission","authors":["Kaneko T.","Katoh T.","Asamizu E.","Sato S.","Nakamura Y.","Kotani H.","Tabata S."],"title":"Structural analysis of Arabidopsis thaliana chromosome 5. XI.","publicationDate":"JUN-2000","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"27862469","citationType":"journal article","authors":["Cheng C.Y.","Krishnakumar V.","Chan A.P.","Thibaud-Nissen F.","Schobel S.","Town C.D."],"citationCrossReferences":[{"database":"PubMed","id":"27862469"},{"database":"DOI","id":"10.1111/tpj.13415"}],"title":"Araport11: a complete reannotation of the Arabidopsis thaliana reference genome.","publicationDate":"2017","journal":"Plant J.","firstPage":"789","lastPage":"804","volume":"89"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"CI-CI4AM1G71UC0S","citationType":"submission","authoringGroup":["The Arabidopsis Information Resource (TAIR)"],"title":"Community-consensus reference genome of Arabidopsis thaliana Col-0.","publicationDate":"FEB-2026","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"21593871","citationType":"journal article","authors":["Roppolo D.","De Rybel B.","Denervaud Tendon V.","Pfister A.","Alassimone J.","Vermeer J.E.M.","Yamazaki M.","Stierhof Y.-D.","Beeckman T.","Geldner N."],"citationCrossReferences":[{"database":"PubMed","id":"21593871"},{"database":"DOI","id":"10.1038/nature10070"}],"title":"A novel protein family directs Casparian strip formation in the endodermis.","publicationDate":"2011","journal":"Nature","firstPage":"380","lastPage":"383","volume":"473"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION","DIMERIZATION","INTERACTION WITH CASP1; CASP2; CASP3 AND CASP5"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"24920445","citationType":"journal article","authors":["Roppolo D.","Boeckmann B.","Pfister A.","Boutet E.","Rubio M.C.","Denervaud-Tendon V.","Vermeer J.E.","Gheyselinck J.","Xenarios I.","Geldner N."],"citationCrossReferences":[{"database":"PubMed","id":"24920445"},{"database":"DOI","id":"10.1104/pp.114.239137"}],"title":"Functional and evolutionary analysis of the CASPARIAN STRIP MEMBRANE DOMAIN PROTEIN family.","publicationDate":"2014","journal":"Plant Physiol.","firstPage":"1709","lastPage":"1722","volume":"165"},"referencePositions":["GENE FAMILY","NOMENCLATURE"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AP002544","properties":[{"key":"ProteinId","value":"BAB09685.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP002688","properties":[{"key":"ProteinId","value":"AED90983.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"OZ408687","properties":[{"key":"ProteinId","value":"CAO7051246.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"NP_196238.1","properties":[{"key":"NucleotideSequenceId","value":"NM_120702.2"}]},{"database":"AlphaFoldDB","id":"Q9FFZ7","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9FFZ7","properties":[{"key":"Description","value":"-"}]},{"database":"DIP","id":"DIP-59180N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q9FFZ7","properties":[{"key":"Number of interactors","value":"194"}]},{"database":"IntAct","id":"Q9FFZ7","properties":[{"key":"Interactions","value":"4"}]},{"database":"STRING","id":"3702.Q9FFZ7","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"3702-Q9FFZ7","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"223882","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"830507","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ath:AT5G06200","properties":[{"key":"Description","value":"-"}]},{"database":"Araport","id":"AT5G06200","properties":[{"key":"Description","value":"-"}]},{"database":"TAIR","id":"AT5G06200","properties":[{"key":"GeneName","value":"CASP4"}]},{"database":"eggNOG","id":"ENOG502RYTF","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_066104_3_1_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q9FFZ7","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"TSANWIA","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"753675at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q9FFZ7","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:Q9FFZ7","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000006548","properties":[{"key":"Component","value":"Chromosome 5"}]},{"database":"ExpressionAtlas","id":"Q9FFZ7","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0048226","properties":[{"key":"GoTerm","value":"C:Casparian strip"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"21593871"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"21593871"}]},{"database":"GO","id":"GO:0042803","properties":[{"key":"GoTerm","value":"F:protein homodimerization activity"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"PubMed","id":"21593871"}]},{"database":"GO","id":"GO:0042545","properties":[{"key":"GoTerm","value":"P:cell wall modification"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"21593871"}]},{"database":"GO","id":"GO:0007043","properties":[{"key":"GoTerm","value":"P:cell-cell junction assembly"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"21593871"}]},{"database":"InterPro","id":"IPR006459","properties":[{"key":"EntryName","value":"CASP/CASPL"}]},{"database":"InterPro","id":"IPR006702","properties":[{"key":"EntryName","value":"CASP_dom"}]},{"database":"InterPro","id":"IPR044173","properties":[{"key":"EntryName","value":"CASPL"}]},{"database":"NCBIfam","id":"TIGR01569","properties":[{"key":"EntryName","value":"A_tha_TIGR01569"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR36488:SF11","properties":[{"key":"EntryName","value":"CASP-LIKE PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR36488","properties":[{"key":"EntryName","value":"CASP-LIKE PROTEIN 1U1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04535","properties":[{"key":"EntryName","value":"CASP_dom"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKSDSIAVDVPAESSSVIKGKAPLLGLARDHTGSGGYKRGLSIFDFLLRLAAIVAALAAAATMGTSDETLPFFTQFLQFEASYDDLPTFQFFVVAIAIVAGYLVLSLPFSVVTIVRPLAVAPRLLLLVLDTAALALDTAAASAAAAIVYLAHNGNTNTNWLPICQQFGDFCQKTSGAVVSAFASVTFLAILVVISGVSLKRP","length":202,"molWeight":21112,"crc64":"488DA4E999CF0A0F","md5":"D640965A7AA59F241BD1F4888C25B652"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Topological domain":5,"Transmembrane":4},"uniParcId":"UPI00000A44CF"}}