{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9D0F1","secondaryAccessions":["Q3TQT6","Q3UWM5","Q99P70"],"uniProtkbId":"NDC80_MOUSE","entryAudit":{"firstPublicDate":"2006-09-19","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2001-06-01","entryVersion":165,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Mus musculus","commonName":"Mouse","taxonId":10090,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Glires","Rodentia","Myomorpha","Muroidea","Muridae","Murinae","Mus","Mus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Kinetochore protein NDC80 homolog"}},"alternativeNames":[{"fullName":{"value":"Kinetochore protein Hec1"}},{"fullName":{"value":"Kinetochore-associated protein 2"}}]},"genes":[{"geneName":{"value":"Ndc80"},"synonyms":[{"value":"Hec1"},{"value":"Kntc2"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O14777"}],"value":"Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O14777"}],"value":"Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end. Interacts with NEK2 and ZWINT specifically during mitosis. Interacts with CENPH and MIS12. May interact with AURKB, PSMC2, PSMC5 and SMC1A. May interact with RB1 during G2 phase and mitosis. Interacts with CKAP5 (By similarity). Interacts with CDT1; leading to kinetochore localization of CDT1 (By similarity)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O14777"}],"value":"Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O14777"}],"value":"Nucleus","id":"SL-0191"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O14777"}],"value":"Chromosome, centromere, kinetochore","id":"SL-0149"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9315664"}],"value":"Expressed in spleen, testis and thymus"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O14777"}],"value":"Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. Also phosphorylated by AURKA and AURKB"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O14777"}],"value":"Acetylated at Lys-53 and Lys-59 by KAT5 during mitosis, promoting robust kinetochore-microtubule attachment. Deacetylated by SIRT1"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the NDC80/HEC1 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":642,"modifier":"EXACT"}},"description":"Kinetochore protein NDC80 homolog","featureId":"PRO_0000249552"},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":445,"modifier":"EXACT"}},"description":"Interaction with the N-terminus of CDCA1","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O14777"}]},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":250,"modifier":"EXACT"}},"description":"Nuclear localization","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O14777"}]},{"type":"Region","location":{"start":{"value":128,"modifier":"EXACT"},"end":{"value":251,"modifier":"EXACT"}},"description":"Interaction with RB1","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O14777"}]},{"type":"Region","location":{"start":{"value":251,"modifier":"EXACT"},"end":{"value":618,"modifier":"EXACT"}},"description":"Interaction with NEK2 and ZWINT","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O14777"}]},{"type":"Region","location":{"start":{"value":251,"modifier":"EXACT"},"end":{"value":431,"modifier":"EXACT"}},"description":"Interaction with SMC1A","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O14777"}]},{"type":"Region","location":{"start":{"value":361,"modifier":"EXACT"},"end":{"value":547,"modifier":"EXACT"}},"description":"Interaction with PSMC2 and SMC1A","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O14777"}]},{"type":"Region","location":{"start":{"value":446,"modifier":"EXACT"},"end":{"value":642,"modifier":"EXACT"}},"description":"Interaction with the C-terminus of CDCA1 and the SPBC24-SPBC25 subcomplex","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O14777"}]},{"type":"Coiled coil","location":{"start":{"value":240,"modifier":"EXACT"},"end":{"value":403,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Coiled coil","location":{"start":{"value":458,"modifier":"EXACT"},"end":{"value":639,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Modified residue","location":{"start":{"value":44,"modifier":"EXACT"},"end":{"value":44,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O14777"}]},{"type":"Modified residue","location":{"start":{"value":53,"modifier":"EXACT"},"end":{"value":53,"modifier":"EXACT"}},"description":"N6-acetyllysine","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O14777"}]},{"type":"Modified residue","location":{"start":{"value":55,"modifier":"EXACT"},"end":{"value":55,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O14777"}]},{"type":"Modified residue","location":{"start":{"value":59,"modifier":"EXACT"},"end":{"value":59,"modifier":"EXACT"}},"description":"N6-acetyllysine","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O14777"}]},{"type":"Modified residue","location":{"start":{"value":62,"modifier":"EXACT"},"end":{"value":62,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O14777"}]},{"type":"Modified residue","location":{"start":{"value":69,"modifier":"EXACT"},"end":{"value":69,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O14777"}]},{"type":"Modified residue","location":{"start":{"value":165,"modifier":"EXACT"},"end":{"value":165,"modifier":"EXACT"}},"description":"Phosphoserine; by NEK2","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O14777"}]},{"type":"Modified residue","location":{"start":{"value":242,"modifier":"EXACT"},"end":{"value":242,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"21183079"}]},{"type":"Modified residue","location":{"start":{"value":527,"modifier":"EXACT"},"end":{"value":527,"modifier":"EXACT"}},"description":"N6-acetyllysine","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O14777"}]},{"type":"Sequence conflict","location":{"start":{"value":13,"modifier":"EXACT"},"end":{"value":13,"modifier":"EXACT"}},"description":"in Ref. 2; BAE37296","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["S"]}},{"type":"Sequence conflict","location":{"start":{"value":274,"modifier":"EXACT"},"end":{"value":274,"modifier":"EXACT"}},"description":"in Ref. 1; AAK01425","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"A","alternativeSequences":["E"]}}],"keywords":[{"id":"KW-0007","category":"PTM","name":"Acetylation"},{"id":"KW-0131","category":"Biological process","name":"Cell cycle"},{"id":"KW-0132","category":"Biological process","name":"Cell division"},{"id":"KW-0137","category":"Cellular component","name":"Centromere"},{"id":"KW-0158","category":"Cellular component","name":"Chromosome"},{"id":"KW-0175","category":"Domain","name":"Coiled coil"},{"id":"KW-0995","category":"Cellular component","name":"Kinetochore"},{"id":"KW-0498","category":"Biological process","name":"Mitosis"},{"id":"KW-0539","category":"Cellular component","name":"Nucleus"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"11266451","citationType":"journal article","authors":["Wigge P.A.","Kilmartin J.V."],"citationCrossReferences":[{"database":"PubMed","id":"11266451"},{"database":"DOI","id":"10.1083/jcb.152.2.349"}],"title":"The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation.","publicationDate":"2001","journal":"J. Cell Biol.","firstPage":"349","lastPage":"360","volume":"152"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]"]},{"referenceNumber":2,"citation":{"id":"16141072","citationType":"journal article","authors":["Carninci P.","Kasukawa T.","Katayama S.","Gough J.","Frith M.C.","Maeda N.","Oyama R.","Ravasi T.","Lenhard B.","Wells C.","Kodzius R.","Shimokawa K.","Bajic V.B.","Brenner S.E.","Batalov S.","Forrest A.R.","Zavolan M.","Davis M.J.","Wilming L.G.","Aidinis V.","Allen J.E.","Ambesi-Impiombato A.","Apweiler R.","Aturaliya R.N.","Bailey T.L.","Bansal M.","Baxter L.","Beisel K.W.","Bersano T.","Bono H.","Chalk A.M.","Chiu K.P.","Choudhary V.","Christoffels A.","Clutterbuck D.R.","Crowe M.L.","Dalla E.","Dalrymple B.P.","de Bono B.","Della Gatta G.","di Bernardo D.","Down T.","Engstrom P.","Fagiolini M.","Faulkner G.","Fletcher C.F.","Fukushima T.","Furuno M.","Futaki S.","Gariboldi M.","Georgii-Hemming P.","Gingeras T.R.","Gojobori T.","Green R.E.","Gustincich S.","Harbers M.","Hayashi Y.","Hensch T.K.","Hirokawa N.","Hill D.","Huminiecki L.","Iacono M.","Ikeo K.","Iwama A.","Ishikawa T.","Jakt M.","Kanapin A.","Katoh M.","Kawasawa Y.","Kelso J.","Kitamura H.","Kitano H.","Kollias G.","Krishnan S.P.","Kruger A.","Kummerfeld S.K.","Kurochkin I.V.","Lareau L.F.","Lazarevic D.","Lipovich L.","Liu J.","Liuni S.","McWilliam S.","Madan Babu M.","Madera M.","Marchionni L.","Matsuda H.","Matsuzawa S.","Miki H.","Mignone F.","Miyake S.","Morris K.","Mottagui-Tabar S.","Mulder N.","Nakano N.","Nakauchi H.","Ng P.","Nilsson R.","Nishiguchi S.","Nishikawa S.","Nori F.","Ohara O.","Okazaki Y.","Orlando V.","Pang K.C.","Pavan W.J.","Pavesi G.","Pesole G.","Petrovsky N.","Piazza S.","Reed J.","Reid J.F.","Ring B.Z.","Ringwald M.","Rost B.","Ruan Y.","Salzberg S.L.","Sandelin A.","Schneider C.","Schoenbach C.","Sekiguchi K.","Semple C.A.","Seno S.","Sessa L.","Sheng Y.","Shibata Y.","Shimada H.","Shimada K.","Silva D.","Sinclair B.","Sperling S.","Stupka E.","Sugiura K.","Sultana R.","Takenaka Y.","Taki K.","Tammoja K.","Tan S.L.","Tang S.","Taylor M.S.","Tegner J.","Teichmann S.A.","Ueda H.R.","van Nimwegen E.","Verardo R.","Wei C.L.","Yagi K.","Yamanishi H.","Zabarovsky E.","Zhu S.","Zimmer A.","Hide W.","Bult C.","Grimmond S.M.","Teasdale R.D.","Liu E.T.","Brusic V.","Quackenbush J.","Wahlestedt C.","Mattick J.S.","Hume D.A.","Kai C.","Sasaki D.","Tomaru Y.","Fukuda S.","Kanamori-Katayama M.","Suzuki M.","Aoki J.","Arakawa T.","Iida J.","Imamura K.","Itoh M.","Kato T.","Kawaji H.","Kawagashira N.","Kawashima T.","Kojima M.","Kondo S.","Konno H.","Nakano K.","Ninomiya N.","Nishio T.","Okada M.","Plessy C.","Shibata K.","Shiraki T.","Suzuki S.","Tagami M.","Waki K.","Watahiki A.","Okamura-Oho Y.","Suzuki H.","Kawai J.","Hayashizaki Y."],"citationCrossReferences":[{"database":"PubMed","id":"16141072"},{"database":"DOI","id":"10.1126/science.1112014"}],"title":"The transcriptional landscape of the mammalian genome.","publicationDate":"2005","journal":"Science","firstPage":"1559","lastPage":"1563","volume":"309"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"C57BL/6J","type":"STRAIN"},{"value":"Egg","type":"TISSUE"},{"value":"Kidney","type":"TISSUE"}]},{"referenceNumber":3,"citation":{"id":"15489334","citationType":"journal article","authoringGroup":["The MGC Project Team"],"citationCrossReferences":[{"database":"PubMed","id":"15489334"},{"database":"DOI","id":"10.1101/gr.2596504"}],"title":"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).","publicationDate":"2004","journal":"Genome Res.","firstPage":"2121","lastPage":"2127","volume":"14"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"FVB/N","type":"STRAIN"},{"value":"Mammary tumor","type":"TISSUE"}]},{"referenceNumber":4,"citation":{"id":"9315664","citationType":"journal article","authors":["Chen Y.","Riley D.J.","Chen P.-L.","Lee W.-H."],"citationCrossReferences":[{"database":"PubMed","id":"9315664"},{"database":"DOI","id":"10.1128/mcb.17.10.6049"}],"title":"HEC, a novel nuclear protein rich in leucine heptad repeats specifically involved in mitosis.","publicationDate":"1997","journal":"Mol. Cell. Biol.","firstPage":"6049","lastPage":"6056","volume":"17"},"referencePositions":["TISSUE SPECIFICITY"]},{"referenceNumber":5,"citation":{"id":"21183079","citationType":"journal article","authors":["Huttlin E.L.","Jedrychowski M.P.","Elias J.E.","Goswami T.","Rad R.","Beausoleil S.A.","Villen J.","Haas W.","Sowa M.E.","Gygi S.P."],"citationCrossReferences":[{"database":"PubMed","id":"21183079"},{"database":"DOI","id":"10.1016/j.cell.2010.12.001"}],"title":"A tissue-specific atlas of mouse protein phosphorylation and expression.","publicationDate":"2010","journal":"Cell","firstPage":"1174","lastPage":"1189","volume":"143"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-242","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"Spleen","type":"TISSUE"},{"value":"Testis","type":"TISSUE"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF326730","properties":[{"key":"ProteinId","value":"AAK01425.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK011497","properties":[{"key":"ProteinId","value":"BAB27657.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK136236","properties":[{"key":"ProteinId","value":"BAE22889.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK163317","properties":[{"key":"ProteinId","value":"BAE37296.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK169427","properties":[{"key":"ProteinId","value":"BAE41169.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC020131","properties":[{"key":"ProteinId","value":"AAH20131.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"CCDS","id":"CCDS28959.1","properties":[{"key":"Description","value":"-"}]},{"database":"RefSeq","id":"NP_075783.2","properties":[{"key":"NucleotideSequenceId","value":"NM_023294.2"}]},{"database":"AlphaFoldDB","id":"Q9D0F1","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9D0F1","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"211904","properties":[{"key":"Interactions","value":"57"}]},{"database":"ComplexPortal","id":"CPX-551","properties":[{"key":"EntryName","value":"Ndc80 complex"}]},{"database":"FunCoup","id":"Q9D0F1","properties":[{"key":"Number of interactors","value":"1337"}]},{"database":"IntAct","id":"Q9D0F1","properties":[{"key":"Interactions","value":"54"}]},{"database":"MINT","id":"Q9D0F1","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"10090.ENSMUSP00000024851","properties":[{"key":"Description","value":"-"}]},{"database":"GlyGen","id":"Q9D0F1","properties":[{"key":"glycosylation","value":"1 site, 1 O-linked glycan (1 site)"}]},{"database":"iPTMnet","id":"Q9D0F1","properties":[{"key":"Description","value":"-"}]},{"database":"PhosphoSitePlus","id":"Q9D0F1","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"10090-ENSMUSP00000024851","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"Q9D0F1","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"293637","properties":[{"key":"Description","value":"-"}]},{"database":"Pumba","id":"Q9D0F1","properties":[{"key":"Description","value":"-"}]},{"database":"Antibodypedia","id":"6039","properties":[{"key":"antibodies","value":"475 antibodies from 42 providers"}]},{"database":"DNASU","id":"67052","properties":[{"key":"Description","value":"-"}]},{"database":"Ensembl","id":"ENSMUST00000024851.10","properties":[{"key":"ProteinId","value":"ENSMUSP00000024851.10"},{"key":"GeneId","value":"ENSMUSG00000024056.11"}]},{"database":"GeneID","id":"67052","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"mmu:67052","properties":[{"key":"Description","value":"-"}]},{"database":"UCSC","id":"uc008dmk.2","properties":[{"key":"OrganismName","value":"mouse"}]},{"database":"AGR","id":"MGI:1914302","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"10403","properties":[{"key":"Description","value":"-"}]},{"database":"MGI","id":"MGI:1914302","properties":[{"key":"GeneName","value":"Ndc80"}]},{"database":"VEuPathDB","id":"HostDB:ENSMUSG00000024056","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG0995","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"GeneTree","id":"ENSGT00390000018386","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_012583_2_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q9D0F1","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"PSHKFQK","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"7459479at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q9D0F1","properties":[{"key":"Description","value":"-"}]},{"database":"Reactome","id":"R-MMU-141444","properties":[{"key":"PathwayName","value":"Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal"}]},{"database":"Reactome","id":"R-MMU-2467813","properties":[{"key":"PathwayName","value":"Separation of Sister Chromatids"}]},{"database":"Reactome","id":"R-MMU-2500257","properties":[{"key":"PathwayName","value":"Resolution of Sister Chromatid Cohesion"}]},{"database":"Reactome","id":"R-MMU-5663220","properties":[{"key":"PathwayName","value":"RHO GTPases Activate Formins"}]},{"database":"Reactome","id":"R-MMU-68877","properties":[{"key":"PathwayName","value":"Mitotic Prometaphase"}]},{"database":"Reactome","id":"R-MMU-9648025","properties":[{"key":"PathwayName","value":"EML4 and NUDC in mitotic spindle formation"}]},{"database":"BioGRID-ORCS","id":"67052","properties":[{"key":"hits","value":"27 hits in 76 CRISPR screens"}]},{"database":"PRO","id":"PR:Q9D0F1","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000589","properties":[{"key":"Component","value":"Chromosome 17"}]},{"database":"RNAct","id":"Q9D0F1","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"Bgee","id":"ENSMUSG00000024056","properties":[{"key":"ExpressionPatterns","value":"Expressed in humerus cartilage element and 135 other cell types or tissues"}]},{"database":"ExpressionAtlas","id":"Q9D0F1","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0005813","properties":[{"key":"GoTerm","value":"C:centrosome"},{"key":"GoEvidenceType","value":"IDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"18940925"}]},{"database":"GO","id":"GO:0000775","properties":[{"key":"GoTerm","value":"C:chromosome, centromeric region"},{"key":"GoEvidenceType","value":"ISO:MGI"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"23541922"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0000776","properties":[{"key":"GoTerm","value":"C:kinetochore"},{"key":"GoEvidenceType","value":"IDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"18940925"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"23541922"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"32483152"}]},{"database":"GO","id":"GO:0031262","properties":[{"key":"GoTerm","value":"C:Ndc80 complex"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0016607","properties":[{"key":"GoTerm","value":"C:nuclear speck"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0005654","properties":[{"key":"GoTerm","value":"C:nucleoplasm"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0005634","properties":[{"key":"GoTerm","value":"C:nucleus"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0000940","properties":[{"key":"GoTerm","value":"C:outer kinetochore"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0030332","properties":[{"key":"GoTerm","value":"F:cyclin binding"},{"key":"GoEvidenceType","value":"IPI:MGI"}],"evidences":[{"evidenceCode":"ECO:0000353","source":"PubMed","id":"23541922"}]},{"database":"GO","id":"GO:0042802","properties":[{"key":"GoTerm","value":"F:identical protein binding"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0140483","properties":[{"key":"GoTerm","value":"F:kinetochore adaptor activity"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0008017","properties":[{"key":"GoTerm","value":"F:microtubule binding"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0051315","properties":[{"key":"GoTerm","value":"P:attachment of mitotic spindle microtubules to kinetochore"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0008608","properties":[{"key":"GoTerm","value":"P:attachment of spindle microtubules to kinetochore"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0051298","properties":[{"key":"GoTerm","value":"P:centrosome duplication"},{"key":"GoEvidenceType","value":"ISO:MGI"}],"evidences":[{"evidenceCode":"ECO:0000266","source":"PubMed","id":"18940925"}]},{"database":"GO","id":"GO:0007059","properties":[{"key":"GoTerm","value":"P:chromosome segregation"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0000132","properties":[{"key":"GoTerm","value":"P:establishment of mitotic spindle orientation"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0008315","properties":[{"key":"GoTerm","value":"P:G2/MI transition of meiotic cell cycle"},{"key":"GoEvidenceType","value":"IDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"23541922"}]},{"database":"GO","id":"GO:0051383","properties":[{"key":"GoTerm","value":"P:kinetochore organization"},{"key":"GoEvidenceType","value":"IMP:MGI"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"23541922"}]},{"database":"GO","id":"GO:0051310","properties":[{"key":"GoTerm","value":"P:metaphase chromosome alignment"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0000070","properties":[{"key":"GoTerm","value":"P:mitotic sister chromatid segregation"},{"key":"GoEvidenceType","value":"ISO:MGI"}]},{"database":"GO","id":"GO:0007094","properties":[{"key":"GoTerm","value":"P:mitotic spindle assembly checkpoint signaling"},{"key":"GoEvidenceType","value":"NAS:ComplexPortal"}],"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"18455984"}]},{"database":"GO","id":"GO:0007052","properties":[{"key":"GoTerm","value":"P:mitotic spindle organization"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0090267","properties":[{"key":"GoTerm","value":"P:positive regulation of mitotic cell cycle spindle assembly checkpoint"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0031647","properties":[{"key":"GoTerm","value":"P:regulation of protein stability"},{"key":"GoEvidenceType","value":"IMP:MGI"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"23541922"}]},{"database":"GO","id":"GO:0014841","properties":[{"key":"GoTerm","value":"P:skeletal muscle satellite cell proliferation"},{"key":"GoEvidenceType","value":"IMP:MGI"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"32483152"}]},{"database":"GO","id":"GO:0007057","properties":[{"key":"GoTerm","value":"P:spindle assembly involved in female meiosis I"},{"key":"GoEvidenceType","value":"IMP:MGI"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"23541922"}]},{"database":"FunFam","id":"1.10.418.30:FF:000002","properties":[{"key":"EntryName","value":"NDC80, kinetochore complex component"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"6.10.250.1950","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.418.30","properties":[{"key":"EntryName","value":"Ncd80 complex, Ncd80 subunit"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR040967","properties":[{"key":"EntryName","value":"DUF5595"}]},{"database":"InterPro","id":"IPR005550","properties":[{"key":"EntryName","value":"Kinetochore_Ndc80"}]},{"database":"InterPro","id":"IPR055260","properties":[{"key":"EntryName","value":"Ndc80_CH"}]},{"database":"InterPro","id":"IPR057091","properties":[{"key":"EntryName","value":"NDC80_loop"}]},{"database":"InterPro","id":"IPR038273","properties":[{"key":"EntryName","value":"Ndc80_sf"}]},{"database":"PANTHER","id":"PTHR10643","properties":[{"key":"EntryName","value":"KINETOCHORE PROTEIN NDC80"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10643:SF2","properties":[{"key":"EntryName","value":"KINETOCHORE PROTEIN NDC80 HOMOLOG"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF18077","properties":[{"key":"EntryName","value":"DUF5595"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03801","properties":[{"key":"EntryName","value":"Ndc80_HEC"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF24487","properties":[{"key":"EntryName","value":"NDC80_loop"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKRSSVSTCGAGRLSMQELRTLDLNKPGLYTPQTKERSTFGKLSTHKPTSERKVSIFGKRTSGHGSRNSQLGIFSSSEKIKDPRPLNDKAFIQQCIRQLYEFLTENGYVYSVSMKSLQAPSTKEFLKIFAFLYGFLCPSYELPGTKCEEEVPRIFKALGYPFTLSKSSMYTVGAPHTWPHIVAALVWLIDCIKIDTAMKESSPLFDDGQLWGEETEDGIKHNKLFLEYTKKCYEKFMTGADSFEEEDAELQAKLKDLYKVDASKLESLEAENKALNEQIARLEEEREREPNRLMSLKKLKASLQADVQNYKAYMSNLESHLAVLKQKSNSLDEEIGRVEQECETVKQENTRLQSIVDNQKYSVADIERINHEKNELQQTINKLTKDLEAEQQQMWNEELKYARGKEAIEAQLAEYHKLARKLKLIPKGAENSKGYDFEIKFNPEAGANCLVKYRTQVYAPLKELLNESEEEINKALNKKRHLEDTLEQLNTMKTESKNTVRMLKEEIQKLDDLHQQAVKEAEEKDKKSASELESLEKHKHLLESGVNDGLSEAMDELDAVQREYQLTVKTTTEERRKVENNLQRLLEMVATHVGSLEKHLEEENAKADREYEEFMSEDLLENIREMAEKYKRNAAQLKAPDK","length":642,"molWeight":73962,"crc64":"2BFF61F09B6E2CF8","md5":"9700CE4E59801B0972D1F26CDC7076D5"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"PTM":2,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Region":7,"Coiled coil":2,"Modified residue":9,"Sequence conflict":2},"uniParcId":"UPI00000E6995"}}