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Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and spindle microtubules (By similarity). The outer kinetochore is made up of the ten-subunit KMN network complex, comprising the MIS12, NDC80 and KNL1 complexes, and auxiliary microtubule-associated components such as the SKA complex; together they connect the outer kinetochore with the inner kinetochore, bind microtubules, and mediate interactions with mitotic checkpoint proteins that delay anaphase until chromosomes are bioriented on the spindle (By similarity). The SKA complex is loaded onto bioriented kinetochores and it facilitates chromosome congression by stabilizing microtubules together with MAPRE1, and end-on attachment of the NDC80 complex to depolymerizing spindle microtubules, thereby assisting the poleward-moving kinetochore in withstanding microtubule pulling forces (By similarity). The complex associates with dynamic microtubule plus-ends and can track both depolymerizing and elongating microtubules (By similarity). The complex recruits protein phosphatase 1 (PP1) to the kinetochore in prometaphase and metaphase, to oppose spindle assembly checkpoint signaling and promote the onset of anaphase (By similarity). In the complex, it mediates interactions with microtubules (By similarity). It also stimulates AURKB/Aurora B catalytic activity (By similarity). During meiosis the SKA complex stabilizes the meiotic spindle and is required for its migration to the cortex (PubMed:22336914)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q96BD8"}],"value":"Component of the SKA complex, composed of SKA1, SKA2 and SKA3. The SKA complex is a homodimer organized around a central W-shaped coiled-coil structure, formed by the interacting domains of SKA1, SKA2, and SKA3, each end of the 'W' is extended further by the C-terminal microtubule-binding domains of SKA1 and SKA3; the complex forms extended structures on microtubules. Interacts (via SXLP motif) with MAPRE1 (via C-terminus); the interaction is direct and stabilizes the kinetochore-microtubule attachment of the SKA1 complex. Interacts (via C-terminus) with protein phosphatase PP1 subunit PPP1CA; the interaction is direct and required for recruitment of PPP1CA to the kinetochore. Interacts with the NDC80 complex; the interaction is required to establish kinetochore-microtubule end-on attachments"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q96BD8"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22336914"}],"value":"Localizes to bioriented kinetochores and spindle microtubules during metaphase in a NDC80 complex-dependent manner (By similarity). The SKA complex begins to concentrate at kinetochores before microtubule attachment but reaches maximum levels on bioriented metaphase chromosomes (By similarity). Localizes both to microtubule plus-ends and along the length of microtubules (By similarity). The localization of the SKA complex to kinetochores is positively regulated by kinase CDK1 (By similarity). The localization of the SKA complex to kinetochores is negatively regulated by protein serine/threonine kinase AURKB, and this action is opposed directly or indirectly by the PP1 and PP2A protein phosphatase complexes (By similarity). Localizes at the centrosome during interphase and prophase (By similarity). Localizes to the meiotic spindle, but not to kinetochores, from the stage of germinal vesicle breakdown (GVBD) to meiosis II (MII) (PubMed:22336914)"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22336914"}],"value":"Cytoplasm, cytoskeleton, spindle","id":"SL-0251"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q96BD8"}],"value":"Chromosome, centromere, kinetochore","id":"SL-0149"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q96BD8"}],"value":"Cytoplasm, cytoskeleton, microtubule organizing center, centrosome","id":"SL-0048"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22336914"}],"value":"Expressed at a constant level in germinal vesicle (GV) stage oocytes, MI-stage oocytes, and MII-stage oocytes"}],"commentType":"DEVELOPMENTAL STAGE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to 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