{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9CA69","secondaryAccessions":["Q8LG71"],"uniProtkbId":"ROH1D_ARATH","entryAudit":{"firstPublicDate":"2023-06-28","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2001-06-01","entryVersion":146,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Arabidopsis thaliana","commonName":"Mouse-ear cress","taxonId":3702,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","rosids","malvids","Brassicales","Brassicaceae","Camelineae","Arabidopsis"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"33519861"}],"value":"Protein ROH1D"}}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"33519861"}],"value":"ROH1D"},"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"Araport","id":"AT1G74450"}],"value":"At1g74450"}],"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AAG52364.1"}],"value":"F1M20.13"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26485022"}],"value":"Involved in the regulation of plant growth, and modulates pollen development to ensure male fertility (PubMed:26485022). May also affect the composition of the inner seed coat mucilage layer (PubMed:26485022)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33519861"}],"value":"Interacts with EXO70C2"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26485022"}],"value":"Present in the cytosol of cells of the root apical meristem and transition zone"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255"}],"value":"Membrane","id":"SL-0162"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255"}],"value":"Single-pass membrane protein","id":"SL-9904"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26485022"}],"value":"Cytoplasm, cytosol","id":"SL-0091"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"26485022"}],"value":"Mostly expressed in mature pollen"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26485022"}],"value":"No visible phenotype"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"'Roh' means corner in Czech"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the ROH1 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":397,"modifier":"EXACT"}},"description":"Protein ROH1D","featureId":"PRO_0000458270"},{"type":"Transmembrane","location":{"start":{"value":247,"modifier":"EXACT"},"end":{"value":267,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Sequence conflict","location":{"start":{"value":146,"modifier":"EXACT"},"end":{"value":146,"modifier":"EXACT"}},"description":"in Ref. 5; AAM61006","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"G","alternativeSequences":["A"]}},{"type":"Sequence conflict","location":{"start":{"value":256,"modifier":"EXACT"},"end":{"value":256,"modifier":"EXACT"}},"description":"in Ref. 5; AAM61006","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["S"]}}],"keywords":[{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"11130712","citationType":"journal article","authors":["Theologis A.","Ecker J.R.","Palm C.J.","Federspiel N.A.","Kaul S.","White O.","Alonso J.","Altafi H.","Araujo R.","Bowman C.L.","Brooks S.Y.","Buehler E.","Chan A.","Chao Q.","Chen H.","Cheuk R.F.","Chin C.W.","Chung M.K.","Conn L.","Conway A.B.","Conway A.R.","Creasy T.H.","Dewar K.","Dunn P.","Etgu P.","Feldblyum T.V.","Feng J.-D.","Fong B.","Fujii C.Y.","Gill J.E.","Goldsmith A.D.","Haas B.","Hansen N.F.","Hughes B.","Huizar L.","Hunter J.L.","Jenkins J.","Johnson-Hopson C.","Khan S.","Khaykin E.","Kim C.J.","Koo H.L.","Kremenetskaia I.","Kurtz D.B.","Kwan A.","Lam B.","Langin-Hooper S.","Lee A.","Lee J.M.","Lenz C.A.","Li J.H.","Li Y.-P.","Lin X.","Liu S.X.","Liu Z.A.","Luros J.S.","Maiti R.","Marziali A.","Militscher J.","Miranda M.","Nguyen M.","Nierman W.C.","Osborne B.I.","Pai G.","Peterson J.","Pham P.K.","Rizzo M.","Rooney T.","Rowley D.","Sakano H.","Salzberg S.L.","Schwartz J.R.","Shinn P.","Southwick A.M.","Sun H.","Tallon L.J.","Tambunga G.","Toriumi M.J.","Town C.D.","Utterback T.","Van Aken S.","Vaysberg M.","Vysotskaia V.S.","Walker M.","Wu D.","Yu G.","Fraser C.M.","Venter J.C.","Davis R.W."],"citationCrossReferences":[{"database":"PubMed","id":"11130712"},{"database":"DOI","id":"10.1038/35048500"}],"title":"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.","publicationDate":"2000","journal":"Nature","firstPage":"816","lastPage":"820","volume":"408"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"27862469","citationType":"journal article","authors":["Cheng C.Y.","Krishnakumar V.","Chan A.P.","Thibaud-Nissen F.","Schobel S.","Town C.D."],"citationCrossReferences":[{"database":"PubMed","id":"27862469"},{"database":"DOI","id":"10.1111/tpj.13415"}],"title":"Araport11: a complete reannotation of the Arabidopsis thaliana reference genome.","publicationDate":"2017","journal":"Plant J.","firstPage":"789","lastPage":"804","volume":"89"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"CI-CI4AM1G71UC0S","citationType":"submission","authoringGroup":["The Arabidopsis Information Resource (TAIR)"],"title":"Community-consensus reference genome of Arabidopsis thaliana Col-0.","publicationDate":"FEB-2026","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"14593172","citationType":"journal article","authors":["Yamada K.","Lim J.","Dale J.M.","Chen H.","Shinn P.","Palm C.J.","Southwick A.M.","Wu H.C.","Kim C.J.","Nguyen M.","Pham P.K.","Cheuk R.F.","Karlin-Newmann G.","Liu S.X.","Lam B.","Sakano H.","Wu T.","Yu G.","Miranda M.","Quach H.L.","Tripp M.","Chang C.H.","Lee J.M.","Toriumi M.J.","Chan M.M.","Tang C.C.","Onodera C.S.","Deng J.M.","Akiyama K.","Ansari Y.","Arakawa T.","Banh J.","Banno F.","Bowser L.","Brooks S.Y.","Carninci P.","Chao Q.","Choy N.","Enju A.","Goldsmith A.D.","Gurjal M.","Hansen N.F.","Hayashizaki Y.","Johnson-Hopson C.","Hsuan V.W.","Iida K.","Karnes M.","Khan S.","Koesema E.","Ishida J.","Jiang P.X.","Jones T.","Kawai J.","Kamiya A.","Meyers C.","Nakajima M.","Narusaka M.","Seki M.","Sakurai T.","Satou M.","Tamse R.","Vaysberg M.","Wallender E.K.","Wong C.","Yamamura Y.","Yuan S.","Shinozaki K.","Davis R.W.","Theologis A.","Ecker J.R."],"citationCrossReferences":[{"database":"PubMed","id":"14593172"},{"database":"DOI","id":"10.1126/science.1088305"}],"title":"Empirical analysis of transcriptional activity in the Arabidopsis genome.","publicationDate":"2003","journal":"Science","firstPage":"842","lastPage":"846","volume":"302"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"CI-3TH3AFLAVIRO9","citationType":"submission","authors":["Brover V.V.","Troukhan M.E.","Alexandrov N.A.","Lu Y.-P.","Flavell R.B.","Feldmann K.A."],"title":"Full-length cDNA from Arabidopsis thaliana.","publicationDate":"MAR-2002","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"]},{"referenceNumber":6,"citation":{"id":"26485022","citationType":"journal article","authors":["Visscher A.M.","Belfield E.J.","Vlad D.","Irani N.","Moore I.","Harberd N.P."],"citationCrossReferences":[{"database":"PubMed","id":"26485022"},{"database":"DOI","id":"10.1371/journal.pone.0140368"}],"title":"Overexpressing the multiple-stress responsive gene At1g74450 reduces plant height and male fertility in Arabidopsis thaliana.","publicationDate":"2015","journal":"PLoS ONE","firstPage":"e0140368","lastPage":"e0140368","volume":"10"},"referencePositions":["FUNCTION","DISRUPTION PHENOTYPE","SUBCELLULAR LOCATION","TISSUE SPECIFICITY"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":7,"citation":{"id":"33519861","citationType":"journal article","authors":["Saccomanno A.","Potocky M.","Pejchar P.","Hala M.","Shikata H.","Schwechheimer C.","Zarsky V."],"citationCrossReferences":[{"database":"PubMed","id":"33519861"},{"database":"DOI","id":"10.3389/fpls.2020.609600"}],"title":"Regulation of exocyst function in pollen tube growth by phosphorylation of exocyst subunit EXO70C2.","publicationDate":"2020","journal":"Front. Plant Sci.","firstPage":"609600","lastPage":"609600","volume":"11"},"referencePositions":["INTERACTION WITH EXO70C2"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AC011765","properties":[{"key":"ProteinId","value":"AAG52364.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP002684","properties":[{"key":"ProteinId","value":"AEE35594.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"OZ408683","properties":[{"key":"ProteinId","value":"CAO7033308.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AF428357","properties":[{"key":"ProteinId","value":"AAL16287.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AY072219","properties":[{"key":"ProteinId","value":"AAL60040.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AY133838","properties":[{"key":"ProteinId","value":"AAM91772.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AY084433","properties":[{"key":"ProteinId","value":"AAM61006.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"PIR","id":"D96773","properties":[{"key":"EntryName","value":"D96773"}]},{"database":"RefSeq","id":"NP_565086.1","properties":[{"key":"NucleotideSequenceId","value":"NM_106105.2"}]},{"database":"AlphaFoldDB","id":"Q9CA69","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q9CA69","properties":[{"key":"Number of interactors","value":"443"}]},{"database":"STRING","id":"3702.Q9CA69","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"Q9CA69","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"3702-Q9CA69","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"183323","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"843786","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ath:AT1G74450","properties":[{"key":"Description","value":"-"}]},{"database":"Araport","id":"AT1G74450","properties":[{"key":"Description","value":"-"}]},{"database":"TAIR","id":"AT1G74450","properties":[{"key":"GeneName","value":"-"}]},{"database":"eggNOG","id":"ENOG502QQUT","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_060027_0_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q9CA69","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"LCQDEFR","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"PRO","id":"PR:Q9CA69","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000006548","properties":[{"key":"Component","value":"Chromosome 1"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IDA:TAIR"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"26485022"}]},{"database":"GO","id":"GO:0016020","properties":[{"key":"GoTerm","value":"C:membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005634","properties":[{"key":"GoTerm","value":"C:nucleus"},{"key":"GoEvidenceType","value":"ISM:TAIR"}]},{"database":"GO","id":"GO:0080001","properties":[{"key":"GoTerm","value":"P:mucilage extrusion from seed coat"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"26485022"}]},{"database":"GO","id":"GO:0009555","properties":[{"key":"GoTerm","value":"P:pollen development"},{"key":"GoEvidenceType","value":"IMP:TAIR"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"26485022"}]},{"database":"GO","id":"GO:0040008","properties":[{"key":"GoTerm","value":"P:regulation of growth"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"26485022"}]},{"database":"InterPro","id":"IPR008511","properties":[{"key":"EntryName","value":"ROH1-like"}]},{"database":"PANTHER","id":"PTHR31509","properties":[{"key":"EntryName","value":"BPS1-LIKE PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF05633","properties":[{"key":"EntryName","value":"ROH1-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MPATEYQRSFGRSFLNLRRDTAVNSVESTTVTPELTQMEAELVSFQRKVAERFIDLNASSCEDLLSLEWVGKLLDSFLSCQEEFRSIVINHRSMITKPPMDRLVSDYFERSVKALDVCNAIRDGVEQIRQWQKLIEIVICAFNNNGGGSSGKRPLGEGQFRRARKTLIELAIGMLDEKDSSSSSVSSQHRNRSFGRNKEQLHHRTIGHFRSLSWSVSRSWSASKQLQAIGNNLATPRASDITATNGLIVPVYTMTTVLLFVMWALVAAIPCQDRGLQVHFNVPRNYQWGGSLMSLHDRIIEESKKRERKNTCGLLKEIHQFEKTSRLMNELVDSVQFPLSEEKEMEVRERVEELGKLQEALKNGLDPFERKVREVFHRIVRSRTEGLDTVGKHHGSE","length":397,"molWeight":45323,"crc64":"E373AD33FA3EE875","md5":"DDC058DD30A6B489FB748424D138A88D"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"DISRUPTION PHENOTYPE":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Transmembrane":1,"Sequence conflict":2},"uniParcId":"UPI00000A4A6E"}}