{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9C809","uniProtkbId":"B3GT8_ARATH","entryAudit":{"firstPublicDate":"2009-01-20","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2001-06-01","entryVersion":128,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Arabidopsis thaliana","commonName":"Mouse-ear cress","taxonId":3702,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","rosids","malvids","Brassicales","Brassicaceae","Camelineae","Arabidopsis"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"value":"Probable beta-1,3-galactosyltransferase 8"},"ecNumbers":[{"value":"2.4.1.-"}]}},"genes":[{"geneName":{"value":"B3GALT8"},"orderedLocusNames":[{"value":"At1g33430"}],"orfNames":[{"value":"F10C21.10"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal glycosyl residue"}],"commentType":"FUNCTION"},{"commentType":"COFACTOR","cofactors":[{"name":"Mn(2+)","evidences":[{"evidenceCode":"ECO:0000250"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29035"}}]},{"texts":[{"value":"Protein modification; protein glycosylation"}],"commentType":"PATHWAY"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Golgi apparatus membrane","id":"SL-0134"},"topology":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Single-pass type II membrane protein","id":"SL-9906"}}]},{"commentType":"ALTERNATIVE PRODUCTS","events":["Alternative splicing"],"isoforms":[{"name":{"value":"1"},"isoformIds":["Q9C809-1"],"isoformSequenceStatus":"Displayed"}],"note":{"texts":[{"value":"A number of isoforms are produced. According to EST sequences."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the glycosyltransferase 31 family"}],"commentType":"SIMILARITY"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Miscellaneous discrepancy","sequence":"BX813522","note":"Sequencing errors.","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":395,"modifier":"EXACT"}},"description":"Probable beta-1,3-galactosyltransferase 8","featureId":"PRO_0000359418"},{"type":"Transmembrane","location":{"start":{"value":5,"modifier":"EXACT"},"end":{"value":27,"modifier":"EXACT"}},"description":"Helical; Signal-anchor for type II membrane protein","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Glycosylation","location":{"start":{"value":117,"modifier":"EXACT"},"end":{"value":117,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""}],"keywords":[{"id":"KW-0025","category":"Coding sequence diversity","name":"Alternative splicing"},{"id":"KW-0325","category":"PTM","name":"Glycoprotein"},{"id":"KW-0328","category":"Molecular function","name":"Glycosyltransferase"},{"id":"KW-0333","category":"Cellular component","name":"Golgi apparatus"},{"id":"KW-0464","category":"Ligand","name":"Manganese"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0735","category":"Domain","name":"Signal-anchor"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"11130712","citationType":"journal article","authors":["Theologis A.","Ecker J.R.","Palm C.J.","Federspiel N.A.","Kaul S.","White O.","Alonso J.","Altafi H.","Araujo R.","Bowman C.L.","Brooks S.Y.","Buehler E.","Chan A.","Chao Q.","Chen H.","Cheuk R.F.","Chin C.W.","Chung M.K.","Conn L.","Conway A.B.","Conway A.R.","Creasy T.H.","Dewar K.","Dunn P.","Etgu P.","Feldblyum T.V.","Feng J.-D.","Fong B.","Fujii C.Y.","Gill J.E.","Goldsmith A.D.","Haas B.","Hansen N.F.","Hughes B.","Huizar L.","Hunter J.L.","Jenkins J.","Johnson-Hopson C.","Khan S.","Khaykin E.","Kim C.J.","Koo H.L.","Kremenetskaia I.","Kurtz D.B.","Kwan A.","Lam B.","Langin-Hooper S.","Lee A.","Lee J.M.","Lenz C.A.","Li J.H.","Li Y.-P.","Lin X.","Liu S.X.","Liu Z.A.","Luros J.S.","Maiti R.","Marziali A.","Militscher J.","Miranda M.","Nguyen M.","Nierman W.C.","Osborne B.I.","Pai G.","Peterson J.","Pham P.K.","Rizzo M.","Rooney T.","Rowley D.","Sakano H.","Salzberg S.L.","Schwartz J.R.","Shinn P.","Southwick A.M.","Sun H.","Tallon L.J.","Tambunga G.","Toriumi M.J.","Town C.D.","Utterback T.","Van Aken S.","Vaysberg M.","Vysotskaia V.S.","Walker M.","Wu D.","Yu G.","Fraser C.M.","Venter J.C.","Davis R.W."],"citationCrossReferences":[{"database":"PubMed","id":"11130712"},{"database":"DOI","id":"10.1038/35048500"}],"title":"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.","publicationDate":"2000","journal":"Nature","firstPage":"816","lastPage":"820","volume":"408"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"27862469","citationType":"journal article","authors":["Cheng C.Y.","Krishnakumar V.","Chan A.P.","Thibaud-Nissen F.","Schobel S.","Town C.D."],"citationCrossReferences":[{"database":"PubMed","id":"27862469"},{"database":"DOI","id":"10.1111/tpj.13415"}],"title":"Araport11: a complete reannotation of the Arabidopsis thaliana reference genome.","publicationDate":"2017","journal":"Plant J.","firstPage":"789","lastPage":"804","volume":"89"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"CI-CI4AM1G71UC0S","citationType":"submission","authoringGroup":["The Arabidopsis Information Resource (TAIR)"],"title":"Community-consensus reference genome of Arabidopsis thaliana Col-0.","publicationDate":"FEB-2026","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"14993207","citationType":"journal article","authors":["Castelli V.","Aury J.-M.","Jaillon O.","Wincker P.","Clepet C.","Menard M.","Cruaud C.","Quetier F.","Scarpelli C.","Schaechter V.","Temple G.","Caboche M.","Weissenbach J.","Salanoubat M."],"citationCrossReferences":[{"database":"PubMed","id":"14993207"},{"database":"DOI","id":"10.1101/gr.1515604"}],"title":"Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation.","publicationDate":"2004","journal":"Genome Res.","firstPage":"406","lastPage":"413","volume":"14"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"18548197","citationType":"journal article","authors":["Qu Y.","Egelund J.","Gilson P.R.","Houghton F.","Gleeson P.A.","Schultz C.J.","Bacic A."],"citationCrossReferences":[{"database":"PubMed","id":"18548197"},{"database":"DOI","id":"10.1007/s11103-008-9351-3"}],"title":"Identification of a novel group of putative Arabidopsis thaliana beta-(1,3)-galactosyltransferases.","publicationDate":"2008","journal":"Plant Mol. Biol.","firstPage":"43","lastPage":"59","volume":"68"},"referencePositions":["GENE FAMILY","NOMENCLATURE"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AC051630","properties":[{"key":"ProteinId","value":"AAG51207.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP002684","properties":[{"key":"ProteinId","value":"AEE31593.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"OZ408683","properties":[{"key":"ProteinId","value":"CAO7029537.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"BX813522","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"PIR","id":"A86458","properties":[{"key":"EntryName","value":"A86458"}]},{"database":"RefSeq","id":"NP_174609.1","properties":[{"key":"NucleotideSequenceId","value":"NM_103068.2"}],"isoformId":"Q9C809-1"},{"database":"AlphaFoldDB","id":"Q9C809","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9C809","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"25470","properties":[{"key":"Interactions","value":"1"}]},{"database":"FunCoup","id":"Q9C809","properties":[{"key":"Number of interactors","value":"1642"}]},{"database":"IntAct","id":"Q9C809","properties":[{"key":"Interactions","value":"1"}]},{"database":"STRING","id":"3702.Q9C809","properties":[{"key":"Description","value":"-"}]},{"database":"CAZy","id":"GT31","properties":[{"key":"FamilyName","value":"Glycosyltransferase Family 31"}]},{"database":"GlyCosmos","id":"Q9C809","properties":[{"key":"glycosylation","value":"1 site, No reported glycans"}]},{"database":"GlyGen","id":"Q9C809","properties":[{"key":"glycosylation","value":"2 sites"}]},{"database":"iPTMnet","id":"Q9C809","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"3702-Q9C809","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"840236","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ath:AT1G33430","properties":[{"key":"Description","value":"-"}]},{"database":"Araport","id":"AT1G33430","properties":[{"key":"Description","value":"-"}]},{"database":"TAIR","id":"AT1G33430","properties":[{"key":"GeneName","value":"UPEX1"}]},{"database":"eggNOG","id":"KOG2288","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_040730_3_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q9C809","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q9C809","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00378","properties":[{"key":"RectionId","value":"-"}]},{"database":"PRO","id":"PR:Q9C809","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000006548","properties":[{"key":"Component","value":"Chromosome 1"}]},{"database":"ExpressionAtlas","id":"Q9C809","properties":[{"key":"ExpressionPatterns","value":"differential"}]},{"database":"GO","id":"GO:0005794","properties":[{"key":"GoTerm","value":"C:Golgi apparatus"},{"key":"GoEvidenceType","value":"ISM:TAIR"}]},{"database":"GO","id":"GO:0000139","properties":[{"key":"GoTerm","value":"C:Golgi membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0048531","properties":[{"key":"GoTerm","value":"F:beta-1,3-galactosyltransferase activity"},{"key":"GoEvidenceType","value":"IDA:TAIR"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"27837085"}]},{"database":"GO","id":"GO:0009101","properties":[{"key":"GoTerm","value":"P:glycoprotein biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0010407","properties":[{"key":"GoTerm","value":"P:non-classical arabinogalactan protein metabolic process"},{"key":"GoEvidenceType","value":"IDA:TAIR"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"27837085"}]},{"database":"GO","id":"GO:0010584","properties":[{"key":"GoTerm","value":"P:pollen exine formation"},{"key":"GoEvidenceType","value":"IMP:TAIR"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"21849515"},{"evidenceCode":"ECO:0000315","source":"PubMed","id":"27837085"}]},{"database":"FunFam","id":"3.90.550.50:FF:000002","properties":[{"key":"EntryName","value":"Hexosyltransferase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.90.550.50","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR025298","properties":[{"key":"EntryName","value":"DUF4094"}]},{"database":"InterPro","id":"IPR002659","properties":[{"key":"EntryName","value":"Glyco_trans_31"}]},{"database":"PANTHER","id":"PTHR11214:SF275","properties":[{"key":"EntryName","value":"BETA-1,3-GALACTOSYLTRANSFERASE 8-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11214","properties":[{"key":"EntryName","value":"BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF13334","properties":[{"key":"EntryName","value":"DUF4094"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01762","properties":[{"key":"EntryName","value":"Galactosyl_T"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MRAKAASGKAIIVLCLASFLAGSLFMSRTLSRSYIPEEEDHHLTKHLSKHLEIQKDCDEHKRKLIESKSRDIIGEVSRTHQAVKSLERTMSTLEMELAAARTSDRSSEFWSERSAKNQSRLQKVFAVIGINTAFSSKKRRDSVRQTWMPTGEKLKKIEKEKGIVVRFVIGHSATPGGVLDKAIDEEDSEHKDFLRLKHIEGYHQLSTKTRLYFSTATAMYDAEFYVKVDDDVHVNLGMLVTTLARYQSRPRIYIGCMKSGPVLSQKGVKYHEPEFWKFGEEGNKYFRHATGQIYAISKDLATYISTNQGILHRYANEDVSLGAWMLGLEVEHVDERSMCCGTPPDCQWKAQAGNVCAASFDWSCSGICKSVDRMARVHRACAEGDTPLANFRFFV","length":395,"molWeight":44732,"crc64":"7C289A6881DAB366","md5":"7ECFFE450D19B2B28F630BADC4E207D7"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"COFACTOR":1,"PATHWAY":1,"SUBCELLULAR LOCATION":1,"ALTERNATIVE PRODUCTS":1,"SIMILARITY":1,"SEQUENCE CAUTION":1},"countByFeatureType":{"Chain":1,"Transmembrane":1,"Glycosylation":1},"uniParcId":"UPI000009EB97"}}