{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9BYE2","secondaryAccessions":["B4DTM9","E9PIJ5","E9PRA0","F8WAJ3","J3KQC6","Q1RMF8","Q86YM4","Q96JY8","Q9BYE1"],"uniProtkbId":"TMPSD_HUMAN","entryAudit":{"firstPublicDate":"2005-03-15","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2019-04-10","entryVersion":175,"sequenceVersion":5},"annotationScore":5.0,"organism":{"scientificName":"Homo sapiens","commonName":"Human","taxonId":9606,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Primates","Haplorrhini","Catarrhini","Hominidae","Homo"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Transmembrane protease serine 13"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34562451"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35796294"}],"value":"3.4.21.-"}]},"alternativeNames":[{"fullName":{"value":"Membrane-type mosaic serine protease"},"shortNames":[{"value":"Mosaic serine protease"}]}]},"genes":[{"geneName":{"value":"TMPRSS13"},"synonyms":[{"value":"MSP"},{"value":"TMPRSS11"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q5U405"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20977675"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28710277"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34562451"}],"value":"Serine protease (PubMed:20977675, PubMed:28710277, PubMed:34562451). Cleaves the proform of PRSS8/prostasin to form the active protein (PubMed:34562451). Cleaves the proform of HGF to form the active protein which promotes MAPK signaling (PubMed:20977675). Promotes the formation of the stratum corneum and subsequently the epidermal barrier in embryos (By similarity)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20977675"}],"value":"Cleavage of HGF is inhibited by SPINT1/HAI-1 via the BPTI/Kunitz inhibitor 1 domain"}],"commentType":"ACTIVITY REGULATION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28710277"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34562451"}],"value":"Interacts with SPINT1/HAI-1; the interaction promotes the phosphorylation and cell membrane localization of TMPRSS13 (PubMed:28710277). Interacts with SPINT2/HAI-2; the interaction promotes the phosphorylation and cell membrane localization of TMPRSS13 (PubMed:28710277, PubMed:34562451)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28710277"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34562451"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35796294"}],"value":"The non-phosphorylated, inactive full length protein localizes intracellularly (PubMed:28710277). N-glycosylation and phosphorylation is required for trafficking to the cell surface (PubMed:28710277, PubMed:34562451, PubMed:35796294). Interaction with SPINT1/HAI-1 and SPINT2/HAI-2 facilitate its translocation to the cell surface (PubMed:34562451, PubMed:35796294). Proteolytic cleavage is required for secretion (PubMed:35796294)"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28710277"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34562451"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35796294"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255"}],"value":"Single-pass type II membrane protein","id":"SL-9906"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35796294"}],"value":"Secreted","id":"SL-0243"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28710277"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"commentType":"ALTERNATIVE PRODUCTS","events":["Alternative splicing"],"isoforms":[{"name":{"value":"1"},"synonyms":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"11267681"}],"value":"MSPL"}],"isoformIds":["Q9BYE2-1"],"isoformSequenceStatus":"Displayed"},{"name":{"value":"2"},"isoformIds":["Q9BYE2-2"],"sequenceIds":["VSP_013103","VSP_013104"],"isoformSequenceStatus":"Described"},{"name":{"value":"3"},"synonyms":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"11267681"}],"value":"MSPS"}],"isoformIds":["Q9BYE2-3"],"sequenceIds":["VSP_013099","VSP_013102"],"isoformSequenceStatus":"Described"},{"name":{"value":"4"},"isoformIds":["Q9BYE2-4"],"sequenceIds":["VSP_013100","VSP_013101"],"isoformSequenceStatus":"Described"},{"name":{"value":"6"},"isoformIds":["Q9BYE2-6"],"sequenceIds":["VSP_013102"],"isoformSequenceStatus":"Described"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20977675"}],"value":"Expressed in placenta"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11267681"}],"value":"Predominantly expressed in lung, placenta, pancreas, and prostate"}],"commentType":"TISSUE SPECIFICITY","molecule":"Isoform 1"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11267681"}],"value":"Expressed in lung, placenta, pancreas, and prostate (PubMed:11267681). Weakly expressed in testis and peripheral blood lymphocytes (PubMed:11267681)"}],"commentType":"TISSUE SPECIFICITY","molecule":"Isoform 3"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28710277"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34562451"}],"value":"The inactive zymogen is post-translationally modified and then trafficked to the cell surface, whereby it undergoes autocatalytic cleavage resulting in an activated form that is released extracellularly"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28710277"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34562451"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35796294"}],"value":"Phosphorylation is required for localization at the cell surface (PubMed:28710277, PubMed:34562451). Phosphorylation increases following inhibition of protease activity by SPINT2/HAI-2 (PubMed:35796294)"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28710277"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34562451"}],"value":"N-glycosylation of Asn-405 and Asn-445 is required for exit from the endoplasmic reticulum and trafficking to the cell surface (PubMed:28710277, PubMed:34562451). Also required for autocleavage of the zymogen, activation and secretion of the mature protein (PubMed:34562451)"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11267681"}],"value":"The repeat A-S-P-A-[GLQR] is polymorphic and the number of copies varies between 12 to 14"}],"commentType":"POLYMORPHISM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00274"}],"value":"Belongs to the peptidase S1 family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Was termed TMPRSS6 (Ref.3)"}],"commentType":"CAUTION"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":586,"modifier":"EXACT"}},"description":"Transmembrane protease serine 13","featureId":"PRO_0000088698"},{"type":"Topological domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":165,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":166,"modifier":"EXACT"},"end":{"value":186,"modifier":"EXACT"}},"description":"Helical; Signal-anchor for type II membrane protein","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":187,"modifier":"EXACT"},"end":{"value":586,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Repeat","location":{"start":{"value":9,"modifier":"EXACT"},"end":{"value":13,"modifier":"EXACT"}},"description":"1-1"},{"type":"Repeat","location":{"start":{"value":14,"modifier":"EXACT"},"end":{"value":18,"modifier":"EXACT"}},"description":"2-1; approximate"},{"type":"Repeat","location":{"start":{"value":19,"modifier":"EXACT"},"end":{"value":23,"modifier":"EXACT"}},"description":"1-2"},{"type":"Repeat","location":{"start":{"value":24,"modifier":"EXACT"},"end":{"value":28,"modifier":"EXACT"}},"description":"1-3"},{"type":"Repeat","location":{"start":{"value":29,"modifier":"EXACT"},"end":{"value":33,"modifier":"EXACT"}},"description":"2-2"},{"type":"Repeat","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":38,"modifier":"EXACT"}},"description":"1-4"},{"type":"Repeat","location":{"start":{"value":39,"modifier":"EXACT"},"end":{"value":43,"modifier":"EXACT"}},"description":"1-5"},{"type":"Repeat","location":{"start":{"value":44,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"1-6"},{"type":"Repeat","location":{"start":{"value":49,"modifier":"EXACT"},"end":{"value":53,"modifier":"EXACT"}},"description":"2-3"},{"type":"Repeat","location":{"start":{"value":54,"modifier":"EXACT"},"end":{"value":58,"modifier":"EXACT"}},"description":"1-7"},{"type":"Repeat","location":{"start":{"value":59,"modifier":"EXACT"},"end":{"value":63,"modifier":"EXACT"}},"description":"1-8"},{"type":"Repeat","location":{"start":{"value":64,"modifier":"EXACT"},"end":{"value":68,"modifier":"EXACT"}},"description":"2-4"},{"type":"Repeat","location":{"start":{"value":69,"modifier":"EXACT"},"end":{"value":78,"modifier":"EXACT"}},"description":"1-9; approximate"},{"type":"Repeat","location":{"start":{"value":79,"modifier":"EXACT"},"end":{"value":83,"modifier":"EXACT"}},"description":"1-10"},{"type":"Repeat","location":{"start":{"value":84,"modifier":"EXACT"},"end":{"value":88,"modifier":"EXACT"}},"description":"1-11"},{"type":"Repeat","location":{"start":{"value":89,"modifier":"EXACT"},"end":{"value":93,"modifier":"EXACT"}},"description":"1-12"},{"type":"Domain","location":{"start":{"value":195,"modifier":"EXACT"},"end":{"value":325,"modifier":"EXACT"}},"description":"SRCR","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00196"}]},{"type":"Domain","location":{"start":{"value":204,"modifier":"EXACT"},"end":{"value":226,"modifier":"EXACT"}},"description":"LDL-receptor class A"},{"type":"Domain","location":{"start":{"value":326,"modifier":"EXACT"},"end":{"value":559,"modifier":"EXACT"}},"description":"Peptidase S1","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00274"}]},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":115,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":9,"modifier":"EXACT"},"end":{"value":93,"modifier":"EXACT"}},"description":"13 X 5 AA repeats of A-S-P-A-[GLQR]"},{"type":"Region","location":{"start":{"value":14,"modifier":"EXACT"},"end":{"value":68,"modifier":"EXACT"}},"description":"4 X 5 AA repeats of T-P-P-G-R"},{"type":"Region","location":{"start":{"value":131,"modifier":"EXACT"},"end":{"value":157,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":565,"modifier":"EXACT"},"end":{"value":586,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":14,"modifier":"EXACT"},"end":{"value":52,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":69,"modifier":"EXACT"},"end":{"value":111,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":133,"modifier":"EXACT"},"end":{"value":144,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":565,"modifier":"EXACT"},"end":{"value":574,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":575,"modifier":"EXACT"},"end":{"value":586,"modifier":"EXACT"}},"description":"Gly residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Active site","location":{"start":{"value":366,"modifier":"EXACT"},"end":{"value":366,"modifier":"EXACT"}},"description":"Charge relay system","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Active site","location":{"start":{"value":414,"modifier":"EXACT"},"end":{"value":414,"modifier":"EXACT"}},"description":"Charge relay system","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Active site","location":{"start":{"value":511,"modifier":"EXACT"},"end":{"value":511,"modifier":"EXACT"}},"description":"Charge relay system","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34562451"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35796294"}]},{"type":"Site","location":{"start":{"value":228,"modifier":"EXACT"},"end":{"value":229,"modifier":"EXACT"}},"description":"Cleavage; by autolysis; required for cell surface localization and secretion","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35796294"}]},{"type":"Site","location":{"start":{"value":325,"modifier":"EXACT"},"end":{"value":325,"modifier":"EXACT"}},"description":"Required for autocleavage and PRSS8 cleavage","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34562451"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35796294"}]},{"type":"Glycosylation","location":{"start":{"value":255,"modifier":"EXACT"},"end":{"value":255,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":292,"modifier":"EXACT"},"end":{"value":292,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":405,"modifier":"EXACT"},"end":{"value":405,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34562451"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":445,"modifier":"EXACT"},"end":{"value":445,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34562451"}],"featureId":""},{"type":"Disulfide bond","location":{"start":{"value":250,"modifier":"EXACT"},"end":{"value":314,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Disulfide bond","location":{"start":{"value":263,"modifier":"EXACT"},"end":{"value":317,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Disulfide bond","location":{"start":{"value":351,"modifier":"EXACT"},"end":{"value":367,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Disulfide bond","location":{"start":{"value":448,"modifier":"EXACT"},"end":{"value":517,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Disulfide bond","location":{"start":{"value":480,"modifier":"EXACT"},"end":{"value":496,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Disulfide bond","location":{"start":{"value":507,"modifier":"EXACT"},"end":{"value":535,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Alternative sequence","location":{"start":{"value":151,"modifier":"EXACT"},"end":{"value":186,"modifier":"EXACT"}},"description":"in isoform 3","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"11267681"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"14702039"}],"featureId":"VSP_013099","alternativeSequence":{"originalSequence":"GTSLPKFTWREGQKQLPLIGCVLLLIALVVSLIILF","alternativeSequences":["V"]}},{"type":"Alternative sequence","location":{"start":{"value":428,"modifier":"EXACT"},"end":{"value":491,"modifier":"EXACT"}},"description":"in isoform 4","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"14702039"}],"featureId":"VSP_013100","alternativeSequence":{"originalSequence":"AHIHPACLPMHGQTFSLNETCWITGFGKTRETDDKTSPFLREVQVNLIDFKKCNDYLVYDSYLT","alternativeSequences":["GEGICTPRSPAPQPQHPLQPSHLSASVNSYPGPKASAGQKSKTLKDPYMEHFCFIIRETEAQGL"]}},{"type":"Alternative sequence","location":{"start":{"value":492,"modifier":"EXACT"},"end":{"value":586,"modifier":"EXACT"}},"description":"in isoform 4","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"14702039"}],"featureId":"VSP_013101","alternativeSequence":{}},{"type":"Alternative sequence","location":{"start":{"value":560,"modifier":"EXACT"},"end":{"value":586,"modifier":"EXACT"}},"description":"in isoform 3 and isoform 6","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"11267681"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"15489334"}],"featureId":"VSP_013102","alternativeSequence":{"originalSequence":"VRSLQQDTAPSRLGTSSGGDPGGAPRL","alternativeSequences":["SEVRFRKS"]}},{"type":"Alternative sequence","location":{"start":{"value":560,"modifier":"EXACT"},"end":{"value":563,"modifier":"EXACT"}},"description":"in isoform 2","evidences":[{"evidenceCode":"ECO:0000303","source":"Reference","id":"Ref.3"}],"featureId":"VSP_013103","alternativeSequence":{"originalSequence":"VRSL","alternativeSequences":["SSAG"]}},{"type":"Alternative sequence","location":{"start":{"value":564,"modifier":"EXACT"},"end":{"value":586,"modifier":"EXACT"}},"description":"in isoform 2","evidences":[{"evidenceCode":"ECO:0000303","source":"Reference","id":"Ref.3"}],"featureId":"VSP_013104","alternativeSequence":{}},{"type":"Natural variant","location":{"start":{"value":73,"modifier":"EXACT"},"end":{"value":73,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"11267681"}],"featureId":"VAR_081354","alternativeSequence":{"originalSequence":"R","alternativeSequences":["RASPAQ"]}},{"type":"Natural variant","location":{"start":{"value":74,"modifier":"EXACT"},"end":{"value":78,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"11267681"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"15489334"},{"evidenceCode":"ECO:0000303","source":"Reference","id":"Ref.3"}],"featureId":"VAR_081355","alternativeSequence":{}},{"type":"Mutagenesis","location":{"start":{"value":196,"modifier":"EXACT"},"end":{"value":196,"modifier":"EXACT"}},"description":"No effect on autocleavage.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35796294"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["Q"]}},{"type":"Mutagenesis","location":{"start":{"value":201,"modifier":"EXACT"},"end":{"value":201,"modifier":"EXACT"}},"description":"No effect on autocleavage.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35796294"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["Q"]}},{"type":"Mutagenesis","location":{"start":{"value":210,"modifier":"EXACT"},"end":{"value":210,"modifier":"EXACT"}},"description":"No effect on autocleavage.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35796294"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["Q"]}},{"type":"Mutagenesis","location":{"start":{"value":228,"modifier":"EXACT"},"end":{"value":228,"modifier":"EXACT"}},"description":"Significantly reduces autocleavage and cleavage of substrates. Abolishes phosphorylation when inhibited by SPINT2/HAI-2, also reduces localization to the cell surface and is instead localized to the endoplasmic reticulum.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35796294"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["Q"]}},{"type":"Mutagenesis","location":{"start":{"value":255,"modifier":"EXACT"},"end":{"value":255,"modifier":"EXACT"}},"description":"No effect on autocleavage. No effect on PRSS8 cleavage and activation.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34562451"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["Q"]}},{"type":"Mutagenesis","location":{"start":{"value":292,"modifier":"EXACT"},"end":{"value":292,"modifier":"EXACT"}},"description":"No effect on autocleavage. No effect on PRSS8 cleavage and activation.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34562451"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["Q"]}},{"type":"Mutagenesis","location":{"start":{"value":325,"modifier":"EXACT"},"end":{"value":325,"modifier":"EXACT"}},"description":"Abolishes autocleavage. Abolishes PRSS8 cleavage and activation. Increases localization to the cell surface. No effect on glycosylation.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28710277"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34562451"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35796294"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["Q"]}},{"type":"Mutagenesis","location":{"start":{"value":405,"modifier":"EXACT"},"end":{"value":405,"modifier":"EXACT"}},"description":"Loss of localization to the cell surface even in the presence of the inhibitor SPINT2. Reduces PRSS8 cleavage and activation. No effect on interaction with SPINT2.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34562451"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["Q"]}},{"type":"Mutagenesis","location":{"start":{"value":445,"modifier":"EXACT"},"end":{"value":445,"modifier":"EXACT"}},"description":"Reduces autocleavage. Loss of localization to the cell surface even in the presence of the inhibitor SPINT2. Reduces PRSS8 cleavage and activation. No effect on interaction with SPINT2.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34562451"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["Q"]}},{"type":"Mutagenesis","location":{"start":{"value":511,"modifier":"EXACT"},"end":{"value":511,"modifier":"EXACT"}},"description":"Abolishes autocleavage. Abolishes serine protease activity including PRSS8 cleavage and activation. Increases localization to the cell surface. No effect on glycosylation.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28710277"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34562451"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35796294"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["A"]}},{"type":"Sequence conflict","location":{"start":{"value":192,"modifier":"EXACT"},"end":{"value":192,"modifier":"EXACT"}},"description":"in Ref. 3; AAO38062","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["Y"]}},{"type":"Sequence conflict","location":{"start":{"value":206,"modifier":"EXACT"},"end":{"value":206,"modifier":"EXACT"}},"description":"in Ref. 3; AAO38062","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["E"]}},{"type":"Sequence conflict","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"in Ref. 3; AAO38062","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"C","alternativeSequences":["R"]}},{"type":"Sequence conflict","location":{"start":{"value":510,"modifier":"EXACT"},"end":{"value":510,"modifier":"EXACT"}},"description":"in Ref. 4; BAG62041","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["G"]}},{"type":"Sequence conflict","location":{"start":{"value":586,"modifier":"EXACT"},"end":{"value":586,"modifier":"EXACT"}},"description":"in Ref. 1; BAB39741","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"L","alternativeSequences":["V"]}},{"type":"Beta strand","location":{"start":{"value":194,"modifier":"EXACT"},"end":{"value":196,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Beta strand","location":{"start":{"value":212,"modifier":"EXACT"},"end":{"value":214,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Helix","location":{"start":{"value":221,"modifier":"EXACT"},"end":{"value":223,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Beta strand","location":{"start":{"value":227,"modifier":"EXACT"},"end":{"value":230,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Turn","location":{"start":{"value":231,"modifier":"EXACT"},"end":{"value":234,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Beta strand","location":{"start":{"value":235,"modifier":"EXACT"},"end":{"value":240,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Turn","location":{"start":{"value":241,"modifier":"EXACT"},"end":{"value":244,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Beta strand","location":{"start":{"value":245,"modifier":"EXACT"},"end":{"value":247,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Beta strand","location":{"start":{"value":249,"modifier":"EXACT"},"end":{"value":251,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Helix","location":{"start":{"value":257,"modifier":"EXACT"},"end":{"value":265,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Beta strand","location":{"start":{"value":273,"modifier":"EXACT"},"end":{"value":278,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Beta strand","location":{"start":{"value":285,"modifier":"EXACT"},"end":{"value":289,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Beta strand","location":{"start":{"value":292,"modifier":"EXACT"},"end":{"value":294,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Turn","location":{"start":{"value":295,"modifier":"EXACT"},"end":{"value":298,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Beta strand","location":{"start":{"value":299,"modifier":"EXACT"},"end":{"value":302,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Beta strand","location":{"start":{"value":308,"modifier":"EXACT"},"end":{"value":313,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Beta strand","location":{"start":{"value":340,"modifier":"EXACT"},"end":{"value":345,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Beta strand","location":{"start":{"value":348,"modifier":"EXACT"},"end":{"value":357,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Beta strand","location":{"start":{"value":360,"modifier":"EXACT"},"end":{"value":363,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Helix","location":{"start":{"value":366,"modifier":"EXACT"},"end":{"value":369,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Beta strand","location":{"start":{"value":370,"modifier":"EXACT"},"end":{"value":373,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Beta strand","location":{"start":{"value":379,"modifier":"EXACT"},"end":{"value":384,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Beta strand","location":{"start":{"value":397,"modifier":"EXACT"},"end":{"value":402,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Beta strand","location":{"start":{"value":416,"modifier":"EXACT"},"end":{"value":422,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Beta strand","location":{"start":{"value":428,"modifier":"EXACT"},"end":{"value":430,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Helix","location":{"start":{"value":435,"modifier":"EXACT"},"end":{"value":437,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Beta strand","location":{"start":{"value":447,"modifier":"EXACT"},"end":{"value":454,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Beta strand","location":{"start":{"value":468,"modifier":"EXACT"},"end":{"value":475,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Helix","location":{"start":{"value":477,"modifier":"EXACT"},"end":{"value":480,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Turn","location":{"start":{"value":483,"modifier":"EXACT"},"end":{"value":488,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Beta strand","location":{"start":{"value":494,"modifier":"EXACT"},"end":{"value":498,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Beta strand","location":{"start":{"value":514,"modifier":"EXACT"},"end":{"value":519,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Beta strand","location":{"start":{"value":522,"modifier":"EXACT"},"end":{"value":531,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Beta strand","location":{"start":{"value":533,"modifier":"EXACT"},"end":{"value":536,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Beta strand","location":{"start":{"value":542,"modifier":"EXACT"},"end":{"value":546,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Helix","location":{"start":{"value":547,"modifier":"EXACT"},"end":{"value":549,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]},{"type":"Helix","location":{"start":{"value":551,"modifier":"EXACT"},"end":{"value":560,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6KD5"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0025","category":"Coding sequence diversity","name":"Alternative splicing"},{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"id":"KW-0325","category":"PTM","name":"Glycoprotein"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0645","category":"Molecular function","name":"Protease"},{"id":"KW-1267","category":"Technical term","name":"Proteomics identification"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0677","category":"Domain","name":"Repeat"},{"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"id":"KW-0720","category":"Molecular function","name":"Serine protease"},{"id":"KW-0735","category":"Domain","name":"Signal-anchor"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"11267681","citationType":"journal article","authors":["Kim D.R.","Sharmin S.","Inoue M.","Kido H."],"citationCrossReferences":[{"database":"PubMed","id":"11267681"},{"database":"DOI","id":"10.1016/s0167-4781(01)00184-1"}],"title":"Cloning and expression of novel mosaic serine proteases with and without a transmembrane domain from human lung.","publicationDate":"2001","journal":"Biochim. Biophys. Acta","firstPage":"204","lastPage":"209","volume":"1518"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3)","TISSUE SPECIFICITY (ISOFORMS 1 AND 2)","POLYMORPHISM","VARIANTS ALA-SER-PRO-ALA-GLN-73 INS AND 74-ALA--GLN-78 DEL"],"referenceComments":[{"value":"Lung","type":"TISSUE"}]},{"referenceNumber":2,"citation":{"id":"CI-D5NV7JQ9HJ5U5","citationType":"submission","authors":["Kim D.R.","Inoue M.","Kido H."],"publicationDate":"MAR-2007","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["SEQUENCE REVISION TO 192; 259; 298 AND 496"]},{"referenceNumber":3,"citation":{"id":"CI-CD5LUHOGU6IS2","citationType":"submission","authors":["Park T.J.","Park W.J."],"title":"Homo sapiens transmembrane protease, serine 6 (TMPRSS6) mRNA.","publicationDate":"DEC-2002","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2)","VARIANT 74-ALA--GLN-78 DEL"]},{"referenceNumber":4,"citation":{"id":"14702039","citationType":"journal article","authors":["Ota T.","Suzuki Y.","Nishikawa T.","Otsuki T.","Sugiyama T.","Irie R.","Wakamatsu A.","Hayashi K.","Sato H.","Nagai K.","Kimura K.","Makita H.","Sekine M.","Obayashi M.","Nishi T.","Shibahara T.","Tanaka T.","Ishii S.","Yamamoto J.","Saito K.","Kawai Y.","Isono Y.","Nakamura Y.","Nagahari K.","Murakami K.","Yasuda T.","Iwayanagi T.","Wagatsuma M.","Shiratori A.","Sudo H.","Hosoiri T.","Kaku Y.","Kodaira H.","Kondo H.","Sugawara M.","Takahashi M.","Kanda K.","Yokoi T.","Furuya T.","Kikkawa E.","Omura Y.","Abe K.","Kamihara K.","Katsuta N.","Sato K.","Tanikawa M.","Yamazaki M.","Ninomiya K.","Ishibashi T.","Yamashita H.","Murakawa K.","Fujimori K.","Tanai H.","Kimata M.","Watanabe M.","Hiraoka S.","Chiba Y.","Ishida S.","Ono Y.","Takiguchi S.","Watanabe S.","Yosida M.","Hotuta T.","Kusano J.","Kanehori K.","Takahashi-Fujii A.","Hara H.","Tanase T.-O.","Nomura Y.","Togiya S.","Komai F.","Hara R.","Takeuchi K.","Arita M.","Imose N.","Musashino K.","Yuuki H.","Oshima A.","Sasaki N.","Aotsuka S.","Yoshikawa Y.","Matsunawa H.","Ichihara T.","Shiohata N.","Sano S.","Moriya S.","Momiyama H.","Satoh N.","Takami S.","Terashima Y.","Suzuki O.","Nakagawa S.","Senoh A.","Mizoguchi H.","Goto Y.","Shimizu F.","Wakebe H.","Hishigaki H.","Watanabe T.","Sugiyama A.","Takemoto M.","Kawakami B.","Yamazaki M.","Watanabe K.","Kumagai A.","Itakura S.","Fukuzumi Y.","Fujimori Y.","Komiyama M.","Tashiro H.","Tanigami A.","Fujiwara T.","Ono T.","Yamada K.","Fujii Y.","Ozaki K.","Hirao M.","Ohmori Y.","Kawabata A.","Hikiji T.","Kobatake N.","Inagaki H.","Ikema Y.","Okamoto S.","Okitani R.","Kawakami T.","Noguchi S.","Itoh T.","Shigeta K.","Senba T.","Matsumura K.","Nakajima Y.","Mizuno T.","Morinaga M.","Sasaki M.","Togashi T.","Oyama M.","Hata H.","Watanabe M.","Komatsu T.","Mizushima-Sugano J.","Satoh T.","Shirai Y.","Takahashi Y.","Nakagawa K.","Okumura K.","Nagase T.","Nomura N.","Kikuchi H.","Masuho Y.","Yamashita R.","Nakai K.","Yada T.","Nakamura Y.","Ohara O.","Isogai T.","Sugano S."],"citationCrossReferences":[{"database":"PubMed","id":"14702039"},{"database":"DOI","id":"10.1038/ng1285"}],"title":"Complete sequencing and characterization of 21,243 full-length human cDNAs.","publicationDate":"2004","journal":"Nat. Genet.","firstPage":"40","lastPage":"45","volume":"36"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 4 AND 6)"],"referenceComments":[{"value":"Placenta","type":"TISSUE"}]},{"referenceNumber":5,"citation":{"id":"16554811","citationType":"journal article","authors":["Taylor T.D.","Noguchi H.","Totoki Y.","Toyoda A.","Kuroki Y.","Dewar K.","Lloyd C.","Itoh T.","Takeda T.","Kim D.-W.","She X.","Barlow K.F.","Bloom T.","Bruford E.","Chang J.L.","Cuomo C.A.","Eichler E.","FitzGerald M.G.","Jaffe D.B.","LaButti K.","Nicol R.","Park H.-S.","Seaman C.","Sougnez C.","Yang X.","Zimmer A.R.","Zody M.C.","Birren B.W.","Nusbaum C.","Fujiyama A.","Hattori M.","Rogers J.","Lander E.S.","Sakaki Y."],"citationCrossReferences":[{"database":"PubMed","id":"16554811"},{"database":"DOI","id":"10.1038/nature04632"}],"title":"Human chromosome 11 DNA sequence and analysis including novel gene identification.","publicationDate":"2006","journal":"Nature","firstPage":"497","lastPage":"500","volume":"440"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"]},{"referenceNumber":6,"citation":{"id":"15489334","citationType":"journal article","authoringGroup":["The MGC Project Team"],"citationCrossReferences":[{"database":"PubMed","id":"15489334"},{"database":"DOI","id":"10.1101/gr.2596504"}],"title":"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).","publicationDate":"2004","journal":"Genome Res.","firstPage":"2121","lastPage":"2127","volume":"14"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 6)","VARIANT 74-ALA--GLN-78 DEL"]},{"referenceNumber":7,"citation":{"id":"20977675","citationType":"journal article","authors":["Hashimoto T.","Kato M.","Shimomura T.","Kitamura N."],"citationCrossReferences":[{"database":"PubMed","id":"20977675"},{"database":"DOI","id":"10.1111/j.1742-4658.2010.07894.x"}],"title":"TMPRSS13, a type II transmembrane serine protease, is inhibited by hepatocyte growth factor activator inhibitor type 1 and activates pro-hepatocyte growth factor.","publicationDate":"2010","journal":"FEBS J.","firstPage":"4888","lastPage":"4900","volume":"277"},"referencePositions":["FUNCTION","ACTIVITY REGULATION","TISSUE SPECIFICITY"]},{"referenceNumber":8,"citation":{"id":"28710277","citationType":"journal article","authors":["Murray A.S.","Varela F.A.","Hyland T.E.","Schoenbeck A.J.","White J.M.","Tanabe L.M.","Todi S.V.","List K."],"citationCrossReferences":[{"database":"PubMed","id":"28710277"},{"database":"DOI","id":"10.1074/jbc.m117.775999"}],"title":"Phosphorylation of the type II transmembrane serine protease, TMPRSS13, in hepatocyte growth factor activator inhibitor-1 and -2-mediated cell-surface localization.","publicationDate":"2017","journal":"J. Biol. Chem.","firstPage":"14867","lastPage":"14884","volume":"292"},"referencePositions":["FUNCTION","INTERACTION WITH SPINT1 AND SPINT2","SUBCELLULAR LOCATION","PHOSPHORYLATION","MUTAGENESIS OF ARG-325 AND SER-511"]},{"referenceNumber":9,"citation":{"id":"34562451","citationType":"journal article","authors":["Martin C.E.","Murray A.S.","Sala-Hamrick K.E.","Mackinder J.R.","Harrison E.C.","Lundgren J.G.","Varela F.A.","List K."],"citationCrossReferences":[{"database":"PubMed","id":"34562451"},{"database":"DOI","id":"10.1016/j.jbc.2021.101227"}],"title":"Posttranslational modifications of serine protease TMPRSS13 regulate zymogen activation, proteolytic activity, and cell surface localization.","publicationDate":"2021","journal":"J. Biol. Chem.","firstPage":"101227","lastPage":"101227","volume":"297"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY","INTERACTION WITH SPINT2","SUBCELLULAR LOCATION","PROTEOLYTIC PROCESSING","PHOSPHORYLATION","GLYCOSYLATION AT ASN-405 AND ASN-445","MUTAGENESIS OF ASN-255; ASN-292; ARG-325; ASN-405; ASN-445 AND SER-511"]},{"referenceNumber":10,"citation":{"id":"35796294","citationType":"journal article","authors":["Martin C.E.","Murray A.S.","Mackinder J.R.","Sala-Hamrick K.E.","Flynn M.G.","Lundgren J.G.","Varela F.A.","List K."],"citationCrossReferences":[{"database":"PubMed","id":"35796294"},{"database":"DOI","id":"10.1515/hsz-2022-0129"}],"title":"TMPRSS13 zymogen activation, surface localization, and shedding is regulated by proteolytic cleavage within the non-catalytic stem region.","publicationDate":"2022","journal":"Biol. Chem.","firstPage":"969","lastPage":"982","volume":"403"},"referencePositions":["CATALYTIC ACTIVITY","SUBCELLULAR LOCATION","PHOSPHORYLATION","MUTAGENESIS OF ARG-196; ARG-201; ARG-210; ARG-228; ARG-325 AND SER-511"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB048796","properties":[{"key":"ProteinId","value":"BAB39741.2"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AB048797","properties":[{"key":"ProteinId","value":"BAB39742.2"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AY190317","properties":[{"key":"ProteinId","value":"AAO38062.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK027798","properties":[{"key":"ProteinId","value":"BAB55376.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK300283","properties":[{"key":"ProteinId","value":"BAG62041.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AP002962","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"BC114928","properties":[{"key":"ProteinId","value":"AAI14929.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"CCDS","id":"CCDS41721.1","properties":[{"key":"Description","value":"-"}],"isoformId":"Q9BYE2-6"},{"database":"CCDS","id":"CCDS55788.1","properties":[{"key":"Description","value":"-"}],"isoformId":"Q9BYE2-3"},{"database":"CCDS","id":"CCDS55789.1","properties":[{"key":"Description","value":"-"}],"isoformId":"Q9BYE2-4"},{"database":"CCDS","id":"CCDS58185.1","properties":[{"key":"Description","value":"-"}],"isoformId":"Q9BYE2-2"},{"database":"RefSeq","id":"NP_001070731.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001077263.3"}],"isoformId":"Q9BYE2-6"},{"database":"RefSeq","id":"NP_001193718.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001206789.2"}],"isoformId":"Q9BYE2-3"},{"database":"RefSeq","id":"NP_001193719.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001206790.2"}],"isoformId":"Q9BYE2-4"},{"database":"RefSeq","id":"NP_001231924.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001244995.2"}],"isoformId":"Q9BYE2-2"},{"database":"PDB","id":"6KD5","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.60 A"},{"key":"Chains","value":"A=192-325, B=326-586"}]},{"database":"PDBsum","id":"6KD5","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"Q9BYE2","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9BYE2","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"123845","properties":[{"key":"Interactions","value":"141"}]},{"database":"FunCoup","id":"Q9BYE2","properties":[{"key":"Number of interactors","value":"66"}]},{"database":"IntAct","id":"Q9BYE2","properties":[{"key":"Interactions","value":"113"}]},{"database":"STRING","id":"9606.ENSP00000434279","properties":[{"key":"Description","value":"-"}]},{"database":"MEROPS","id":"S01.087","properties":[{"key":"Description","value":"-"}]},{"database":"TCDB","id":"8.A.131.1.13","properties":[{"key":"FamilyName","value":"the transmembrane protease serine 3 (tmprss3) family"}]},{"database":"GlyCosmos","id":"Q9BYE2","properties":[{"key":"glycosylation","value":"4 sites, No reported glycans"}]},{"database":"GlyGen","id":"Q9BYE2","properties":[{"key":"glycosylation","value":"8 sites, 1 O-linked glycan (1 site)"}]},{"database":"iPTMnet","id":"Q9BYE2","properties":[{"key":"Description","value":"-"}]},{"database":"PhosphoSitePlus","id":"Q9BYE2","properties":[{"key":"Description","value":"-"}]},{"database":"SwissPalm","id":"Q9BYE2","properties":[{"key":"Description","value":"-"}]},{"database":"BioMuta","id":"TMPRSS13","properties":[{"key":"Description","value":"-"}]},{"database":"DMDM","id":"313104278","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"Q9BYE2","properties":[{"key":"Description","value":"-"}]},{"database":"MassIVE","id":"Q9BYE2","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"9606-ENSP00000434279","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"Q9BYE2","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"20829","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"23254","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"30511","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"79624","properties":[{"key":"Description","value":"-"}],"isoformId":"Q9BYE2-1"},{"database":"ProteomicsDB","id":"79625","properties":[{"key":"Description","value":"-"}],"isoformId":"Q9BYE2-2"},{"database":"ProteomicsDB","id":"79626","properties":[{"key":"Description","value":"-"}],"isoformId":"Q9BYE2-3"},{"database":"ProteomicsDB","id":"79627","properties":[{"key":"Description","value":"-"}],"isoformId":"Q9BYE2-4"},{"database":"Antibodypedia","id":"32401","properties":[{"key":"antibodies","value":"92 antibodies from 19 providers"}]},{"database":"DNASU","id":"84000","properties":[{"key":"Description","value":"-"}]},{"database":"Ensembl","id":"ENST00000430170.6","properties":[{"key":"ProteinId","value":"ENSP00000387702.2"},{"key":"GeneId","value":"ENSG00000137747.17"}],"isoformId":"Q9BYE2-2"},{"database":"Ensembl","id":"ENST00000445164.6","properties":[{"key":"ProteinId","value":"ENSP00000394114.2"},{"key":"GeneId","value":"ENSG00000137747.17"}],"isoformId":"Q9BYE2-1"},{"database":"Ensembl","id":"ENST00000524993.6","properties":[{"key":"ProteinId","value":"ENSP00000434279.1"},{"key":"GeneId","value":"ENSG00000137747.17"}],"isoformId":"Q9BYE2-6"},{"database":"Ensembl","id":"ENST00000526090.1","properties":[{"key":"ProteinId","value":"ENSP00000436502.1"},{"key":"GeneId","value":"ENSG00000137747.17"}],"isoformId":"Q9BYE2-4"},{"database":"Ensembl","id":"ENST00000528626.5","properties":[{"key":"ProteinId","value":"ENSP00000435813.1"},{"key":"GeneId","value":"ENSG00000137747.17"}],"isoformId":"Q9BYE2-3"},{"database":"GeneID","id":"84000","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"hsa:84000","properties":[{"key":"Description","value":"-"}]},{"database":"MANE-Select","id":"ENST00000524993.6","properties":[{"key":"ProteinId","value":"ENSP00000434279.1"},{"key":"RefSeqNucleotideId","value":"NM_001077263.3"},{"key":"RefSeqProteinId","value":"NP_001070731.1"}],"isoformId":"Q9BYE2-6"},{"database":"UCSC","id":"uc001pru.3","properties":[{"key":"OrganismName","value":"human"}],"isoformId":"Q9BYE2-1"},{"database":"AGR","id":"HGNC:29808","properties":[{"key":"Description","value":"-"}]},{"database":"ClinPGx","id":"PA142670732","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"84000","properties":[{"key":"Description","value":"-"}]},{"database":"DisGeNET","id":"84000","properties":[{"key":"Description","value":"-"}]},{"database":"GeneCards","id":"TMPRSS13","properties":[{"key":"Description","value":"-"}]},{"database":"HGNC","id":"HGNC:29808","properties":[{"key":"GeneName","value":"TMPRSS13"}]},{"database":"HPA","id":"ENSG00000137747","properties":[{"key":"ExpressionPatterns","value":"Tissue enhanced (esophagus, skin)"}]},{"database":"MIM","id":"610050","properties":[{"key":"Type","value":"gene"}]},{"database":"OpenTargets","id":"ENSG00000137747","properties":[{"key":"Description","value":"-"}]},{"database":"VEuPathDB","id":"HostDB:ENSG00000137747","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG3627","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"GeneTree","id":"ENSGT00940000159197","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_006842_19_2_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q9BYE2","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"PWIYSNM","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"10012881at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PAN-GO","id":"Q9BYE2","properties":[{"key":"Number of GO annotations","value":"2 GO annotations based on evolutionary models"}]},{"database":"BRENDA","id":"3.4.21.B61","properties":[{"key":"OrganismId","value":"2681"}]},{"database":"PathwayCommons","id":"Q9BYE2","properties":[{"key":"Description","value":"-"}]},{"database":"SignaLink","id":"Q9BYE2","properties":[{"key":"Description","value":"-"}]},{"database":"Agora","id":"ENSG00000137747","properties":[{"key":"NominatedTarget","value":"-"}]},{"database":"BioGRID-ORCS","id":"84000","properties":[{"key":"hits","value":"14 hits in 1146 CRISPR screens"}]},{"database":"ChiTaRS","id":"TMPRSS13","properties":[{"key":"OrganismName","value":"human"}]},{"database":"GenomeRNAi","id":"84000","properties":[{"key":"Description","value":"-"}]},{"database":"Pharos","id":"Q9BYE2","properties":[{"key":"DevelopmentLevel","value":"Tbio"}]},{"database":"PRO","id":"PR:Q9BYE2","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000005640","properties":[{"key":"Component","value":"Chromosome 11"}]},{"database":"RNAct","id":"Q9BYE2","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"Bgee","id":"ENSG00000137747","properties":[{"key":"ExpressionPatterns","value":"Expressed in upper arm skin and 132 other cell types or tissues"}]},{"database":"ExpressionAtlas","id":"Q9BYE2","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0072562","properties":[{"key":"GoTerm","value":"C:blood microparticle"},{"key":"GoEvidenceType","value":"HDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0007005","source":"PubMed","id":"22516433"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28710277"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35796294"}]},{"database":"GO","id":"GO:0016020","properties":[{"key":"GoTerm","value":"C:membrane"},{"key":"GoEvidenceType","value":"NAS:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"11267681"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28710277"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34562451"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35796294"}]},{"database":"GO","id":"GO:0004252","properties":[{"key":"GoTerm","value":"F:serine-type endopeptidase activity"},{"key":"GoEvidenceType","value":"NAS:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"11267681"}]},{"database":"GO","id":"GO:0008236","properties":[{"key":"GoTerm","value":"F:serine-type peptidase activity"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0016485","properties":[{"key":"GoTerm","value":"P:protein processing"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"CDD","id":"cd00112","properties":[{"key":"EntryName","value":"LDLa"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd00190","properties":[{"key":"EntryName","value":"Tryp_SPc"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.10.250.10:FF:000021","properties":[{"key":"EntryName","value":"Transmembrane serine protease 13"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.40.10.10:FF:000003","properties":[{"key":"EntryName","value":"Transmembrane serine protease 3"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.10.250.10","properties":[{"key":"EntryName","value":"SRCR-like domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.10.10","properties":[{"key":"EntryName","value":"Trypsin-like serine proteases"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR002172","properties":[{"key":"EntryName","value":"LDrepeatLR_classA_rpt"}]},{"database":"InterPro","id":"IPR009003","properties":[{"key":"EntryName","value":"Peptidase_S1_PA"}]},{"database":"InterPro","id":"IPR001314","properties":[{"key":"EntryName","value":"Peptidase_S1A"}]},{"database":"InterPro","id":"IPR017327","properties":[{"key":"EntryName","value":"Peptidase_S1A_TMPRSS13"}]},{"database":"InterPro","id":"IPR001190","properties":[{"key":"EntryName","value":"SRCR"}]},{"database":"InterPro","id":"IPR036772","properties":[{"key":"EntryName","value":"SRCR-like_dom_sf"}]},{"database":"InterPro","id":"IPR001254","properties":[{"key":"EntryName","value":"Trypsin_dom"}]},{"database":"InterPro","id":"IPR018114","properties":[{"key":"EntryName","value":"TRYPSIN_HIS"}]},{"database":"InterPro","id":"IPR033116","properties":[{"key":"EntryName","value":"TRYPSIN_SER"}]},{"database":"PANTHER","id":"PTHR24252","properties":[{"key":"EntryName","value":"ACROSIN-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR24252:SF27","properties":[{"key":"EntryName","value":"TRANSMEMBRANE PROTEASE SERINE 3-LIKE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF15494","properties":[{"key":"EntryName","value":"SRCR_2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00089","properties":[{"key":"EntryName","value":"Trypsin"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF037935","properties":[{"key":"EntryName","value":"TMPRSS13"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00722","properties":[{"key":"EntryName","value":"CHYMOTRYPSIN"}]},{"database":"SMART","id":"SM00202","properties":[{"key":"EntryName","value":"SR"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00020","properties":[{"key":"EntryName","value":"Tryp_SPc"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF56487","properties":[{"key":"EntryName","value":"SRCR-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF50494","properties":[{"key":"EntryName","value":"Trypsin-like serine proteases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50287","properties":[{"key":"EntryName","value":"SRCR_2"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50240","properties":[{"key":"EntryName","value":"TRYPSIN_DOM"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00134","properties":[{"key":"EntryName","value":"TRYPSIN_HIS"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00135","properties":[{"key":"EntryName","value":"TRYPSIN_SER"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MERDSHGNASPARTPSAGASPAQASPAGTPPGRASPAQASPAQASPAGTPPGRASPAQASPAGTPPGRASPGRASPAQASPAQASPARASPALASLSRSSSGRSSSARSASVTTSPTRVYLVRATPVGAVPIRSSPARSAPATRATRESPGTSLPKFTWREGQKQLPLIGCVLLLIALVVSLIILFQFWQGHTGIRYKEQRESCPKHAVRCDGVVDCKLKSDELGCVRFDWDKSLLKIYSGSSHQWLPICSSNWNDSYSEKTCQQLGFESAHRTTEVAHRDFANSFSILRYNSTIQESLHRSECPSQRYISLQCSHCGLRAMTGRIVGGALASDSKWPWQVSLHFGTTHICGGTLIDAQWVLTAAHCFFVTREKVLEGWKVYAGTSNLHQLPEAASIAEIIINSNYTDEEDDYDIALMRLSKPLTLSAHIHPACLPMHGQTFSLNETCWITGFGKTRETDDKTSPFLREVQVNLIDFKKCNDYLVYDSYLTPRMMCAGDLRGGRDSCQGDSGGPLVCEQNNRWYLAGVTSWGTGCGQRNKPGVYTKVTEVLPWIYSKMEVRSLQQDTAPSRLGTSSGGDPGGAPRL","length":586,"molWeight":63167,"crc64":"EDA2E3B7509FB1C6","md5":"5C79F9FE5C6A530F9A6EC12AB52F958E"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"ACTIVITY REGULATION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"ALTERNATIVE PRODUCTS":5,"TISSUE SPECIFICITY":3,"PTM":3,"POLYMORPHISM":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Chain":1,"Topological domain":2,"Transmembrane":1,"Repeat":16,"Domain":3,"Region":5,"Compositional bias":5,"Active site":3,"Site":2,"Glycosylation":4,"Disulfide bond":6,"Alternative sequence":6,"Natural variant":2,"Mutagenesis":10,"Sequence conflict":5,"Beta strand":26,"Helix":7,"Turn":4},"uniParcId":"UPI000049DD54"}}