ID   ADPGK_HUMAN             Reviewed;         497 AA.
AC   Q9BRR6; Q49AU7; Q8NBI1; Q8WZ90; Q96NF8; Q9H0A7;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   27-MAR-2024, entry version 171.
DE   RecName: Full=ADP-dependent glucokinase;
DE            Short=ADP-GK;
DE            Short=ADPGK;
DE            EC=2.7.1.147;
DE   AltName: Full=RbBP-35;
DE   Flags: Precursor;
GN   Name=ADPGK; ORFNames=PSEC0260;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5).
RC   TISSUE=Lymph node;
RA   Fan Z.S., Ao S.Z.;
RT   "RbBP-35 interacted with pRb.";
RL   Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
RC   TISSUE=Testis;
RX   PubMed=11230166; DOI=10.1101/gr.gr1547r;
RA   Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S.,
RA   Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J.,
RA   Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W.,
RA   Ottenwaelder B., Obermaier B., Tampe J., Heubner D., Wambutt R., Korn B.,
RA   Klein M., Poustka A.;
RT   "Towards a catalog of human genes and proteins: sequencing and analysis of
RT   500 novel complete protein coding human cDNAs.";
RL   Genome Res. 11:422-435(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC   TISSUE=Heart;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
RA   Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT   "Cloning of human full open reading frames in Gateway(TM) system entry
RT   vector (pDONR201).";
RL   Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   TISSUE=Teratocarcinoma;
RX   PubMed=16303743; DOI=10.1093/dnares/12.2.117;
RA   Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J.,
RA   Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S.,
RA   Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.,
RA   Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S.,
RA   Isogai T.;
RT   "Signal sequence and keyword trap in silico for selection of full-length
RT   human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA
RT   libraries.";
RL   DNA Res. 12:117-126(2005).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 6).
RC   TISSUE=Brain, and Kidney;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [7]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA   Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA   Bennett K.L., Superti-Furga G., Colinge J.;
RT   "Initial characterization of the human central proteome.";
RL   BMC Syst. Biol. 5:17-17(2011).
RN   [8]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=25944712; DOI=10.1002/pmic.201400617;
RA   Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA   Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT   "N-terminome analysis of the human mitochondrial proteome.";
RL   Proteomics 15:2519-2524(2015).
CC   -!- FUNCTION: Catalyzes the phosphorylation of D-glucose to D-glucose 6-
CC       phosphate using ADP as the phosphate donor. GDP and CDP can replace
CC       ADP, but with reduced efficiency (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ADP + D-glucose = AMP + D-glucose 6-phosphate + H(+);
CC         Xref=Rhea:RHEA:11460, ChEBI:CHEBI:4167, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:61548, ChEBI:CHEBI:456215, ChEBI:CHEBI:456216;
CC         EC=2.7.1.147;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU00584};
CC       Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000255|PROSITE-
CC       ProRule:PRU00584};
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis. {ECO:0000255|PROSITE-
CC       ProRule:PRU00584}.
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=6;
CC       Name=1;
CC         IsoId=Q9BRR6-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q9BRR6-2; Sequence=VSP_013552;
CC       Name=3;
CC         IsoId=Q9BRR6-3; Sequence=VSP_013549;
CC       Name=4;
CC         IsoId=Q9BRR6-4; Sequence=VSP_013550, VSP_013551, VSP_013552;
CC       Name=5;
CC         IsoId=Q9BRR6-5; Sequence=VSP_013548, VSP_013553, VSP_013554;
CC       Name=6;
CC         IsoId=Q9BRR6-6; Sequence=VSP_035014;
CC   -!- SIMILARITY: Belongs to the ADP-dependent glucokinase family.
CC       {ECO:0000305}.
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DR   EMBL; AF204270; AAL31473.1; -; mRNA.
DR   EMBL; AL136873; CAB66807.1; -; mRNA.
DR   EMBL; AK055526; BAB70941.1; -; mRNA.
DR   EMBL; CR533447; CAG38478.1; -; mRNA.
DR   EMBL; AK075560; BAC11699.1; -; mRNA.
DR   EMBL; BC006112; AAH06112.1; -; mRNA.
DR   EMBL; BC018074; AAH18074.1; -; mRNA.
DR   CCDS; CCDS42057.1; -. [Q9BRR6-2]
DR   CCDS; CCDS92041.1; -. [Q9BRR6-1]
DR   RefSeq; NP_112574.3; NM_031284.4. [Q9BRR6-2]
DR   RefSeq; XP_006720775.1; XM_006720712.3.
DR   AlphaFoldDB; Q9BRR6; -.
DR   SMR; Q9BRR6; -.
DR   BioGRID; 123643; 99.
DR   IntAct; Q9BRR6; 33.
DR   MINT; Q9BRR6; -.
DR   STRING; 9606.ENSP00000312250; -.
DR   iPTMnet; Q9BRR6; -.
DR   MetOSite; Q9BRR6; -.
DR   PhosphoSitePlus; Q9BRR6; -.
DR   SwissPalm; Q9BRR6; -.
DR   BioMuta; ADPGK; -.
DR   DMDM; 62899887; -.
DR   EPD; Q9BRR6; -.
DR   jPOST; Q9BRR6; -.
DR   MassIVE; Q9BRR6; -.
DR   MaxQB; Q9BRR6; -.
DR   PaxDb; 9606-ENSP00000312250; -.
DR   PeptideAtlas; Q9BRR6; -.
DR   ProteomicsDB; 78812; -. [Q9BRR6-1]
DR   ProteomicsDB; 78813; -. [Q9BRR6-2]
DR   ProteomicsDB; 78814; -. [Q9BRR6-3]
DR   ProteomicsDB; 78815; -. [Q9BRR6-4]
DR   ProteomicsDB; 78816; -. [Q9BRR6-5]
DR   ProteomicsDB; 78817; -. [Q9BRR6-6]
DR   Pumba; Q9BRR6; -.
DR   Antibodypedia; 26747; 234 antibodies from 30 providers.
DR   DNASU; 83440; -.
DR   Ensembl; ENST00000311669.12; ENSP00000312250.8; ENSG00000159322.18. [Q9BRR6-2]
DR   Ensembl; ENST00000456471.3; ENSP00000397694.3; ENSG00000159322.18. [Q9BRR6-1]
DR   Ensembl; ENST00000562823.1; ENSP00000454367.1; ENSG00000159322.18. [Q9BRR6-6]
DR   Ensembl; ENST00000567941.5; ENSP00000458102.1; ENSG00000159322.18. [Q9BRR6-6]
DR   Ensembl; ENST00000569517.5; ENSP00000454304.1; ENSG00000159322.18. [Q9BRR6-6]
DR   GeneID; 83440; -.
DR   KEGG; hsa:83440; -.
DR   MANE-Select; ENST00000456471.3; ENSP00000397694.3; NM_001365225.1; NP_001352154.1.
DR   UCSC; uc002avf.5; human. [Q9BRR6-1]
DR   AGR; HGNC:25250; -.
DR   CTD; 83440; -.
DR   GeneCards; ADPGK; -.
DR   HGNC; HGNC:25250; ADPGK.
DR   HPA; ENSG00000159322; Low tissue specificity.
DR   MIM; 611861; gene.
DR   neXtProt; NX_Q9BRR6; -.
DR   OpenTargets; ENSG00000159322; -.
DR   PharmGKB; PA134971928; -.
DR   VEuPathDB; HostDB:ENSG00000159322; -.
DR   eggNOG; KOG4184; Eukaryota.
DR   GeneTree; ENSGT00390000017953; -.
DR   HOGENOM; CLU_032362_0_0_1; -.
DR   InParanoid; Q9BRR6; -.
DR   OMA; MWRKASA; -.
DR   OrthoDB; 51859at2759; -.
DR   PhylomeDB; Q9BRR6; -.
DR   TreeFam; TF313401; -.
DR   BRENDA; 2.7.1.147; 2681.
DR   PathwayCommons; Q9BRR6; -.
DR   Reactome; R-HSA-70171; Glycolysis.
DR   SABIO-RK; Q9BRR6; -.
DR   SignaLink; Q9BRR6; -.
DR   UniPathway; UPA00109; -.
DR   BioGRID-ORCS; 83440; 16 hits in 1153 CRISPR screens.
DR   ChiTaRS; ADPGK; human.
DR   GeneWiki; ADP-specific_glucokinase; -.
DR   GenomeRNAi; 83440; -.
DR   Pharos; Q9BRR6; Tbio.
DR   PRO; PR:Q9BRR6; -.
DR   Proteomes; UP000005640; Chromosome 15.
DR   RNAct; Q9BRR6; Protein.
DR   Bgee; ENSG00000159322; Expressed in oocyte and 177 other cell types or tissues.
DR   ExpressionAtlas; Q9BRR6; baseline and differential.
DR   GO; GO:0005783; C:endoplasmic reticulum; IDA:CACAO.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; HDA:UniProtKB.
DR   GO; GO:0043843; F:ADP-specific glucokinase activity; IDA:MGI.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006006; P:glucose metabolic process; IDA:MGI.
DR   GO; GO:0061620; P:glycolytic process through glucose-6-phosphate; TAS:Reactome.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   CDD; cd01938; ADPGK_ADPPFK; 1.
DR   Gene3D; 3.40.1190.20; -; 1.
DR   InterPro; IPR007666; ADP_PFK/GK.
DR   InterPro; IPR029056; Ribokinase-like.
DR   PANTHER; PTHR21208; ADP-DEPENDENT GLUCOKINASE; 1.
DR   PANTHER; PTHR21208:SF0; ADP-DEPENDENT GLUCOKINASE; 1.
DR   Pfam; PF04587; ADP_PFK_GK; 1.
DR   SUPFAM; SSF53613; Ribokinase-like; 1.
DR   PROSITE; PS51255; ADPK; 1.
DR   Genevisible; Q9BRR6; HS.
PE   1: Evidence at protein level;
KW   Alternative splicing; Glycolysis; Kinase; Magnesium; Metal-binding;
KW   Reference proteome; Secreted; Signal; Transferase.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..497
FT                   /note="ADP-dependent glucokinase"
FT                   /id="PRO_0000184776"
FT   DOMAIN          52..497
FT                   /note="ADPK"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00584"
FT   ACT_SITE        481
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00584"
FT   BINDING         297
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00584"
FT   BINDING         328
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00584"
FT   BINDING         481
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00584"
FT   VAR_SEQ         1..274
FT                   /note="Missing (in isoform 4)"
FT                   /evidence="ECO:0000303|PubMed:11230166, ECO:0000303|Ref.4"
FT                   /id="VSP_013550"
FT   VAR_SEQ         1..239
FT                   /note="Missing (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:14702039"
FT                   /id="VSP_013549"
FT   VAR_SEQ         1..122
FT                   /note="Missing (in isoform 5)"
FT                   /evidence="ECO:0000303|Ref.1"
FT                   /id="VSP_013548"
FT   VAR_SEQ         79..497
FT                   /note="Missing (in isoform 6)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_035014"
FT   VAR_SEQ         275..280
FT                   /note="RKRLLE -> MAASCR (in isoform 4)"
FT                   /evidence="ECO:0000303|PubMed:11230166, ECO:0000303|Ref.4"
FT                   /id="VSP_013551"
FT   VAR_SEQ         313
FT                   /note="Missing (in isoform 2 and isoform 4)"
FT                   /evidence="ECO:0000303|PubMed:11230166,
FT                   ECO:0000303|PubMed:16303743, ECO:0000303|Ref.4"
FT                   /id="VSP_013552"
FT   VAR_SEQ         404..469
FT                   /note="AAGARVAGTQACATETIDTSRVSLRAPQEFMTSHSEAGSRIVLNPNKPVVEW
FT                   HREGISFHFTPVLV -> LGSSCGWDTGLRHRNHRHQPSVSEGTPRVHDFPFGGRLQDC
FT                   IKPKQASSRMAQRGNILPLHTSIGV (in isoform 5)"
FT                   /evidence="ECO:0000303|Ref.1"
FT                   /id="VSP_013553"
FT   VAR_SEQ         470..497
FT                   /note="Missing (in isoform 5)"
FT                   /evidence="ECO:0000303|Ref.1"
FT                   /id="VSP_013554"
FT   VARIANT         184
FT                   /note="K -> R (in dbSNP:rs8024644)"
FT                   /id="VAR_060085"
SQ   SEQUENCE   497 AA;  54089 MW;  B758E977CDA88F8F CRC64;
     MALWRGSAYA GFLALAVGCV FLLEPELPGS ALRSLWSSLC LGPAPAPPGP VSPEGRLAAA
     WDALIVRPVR RWRRVAVGVN ACVDVVLSGV KLLQALGLSP GNGKDHSILH SRNDLEEAFI
     HFMGKGAAAE RFFSDKETFH DIAQVASEFP GAQHYVGGNA ALIGQKFAAN SDLKVLLCGP
     VGPKLHELLD DNVFVPPESL QEVDEFHLIL EYQAGEEWGQ LKAPHANRFI FSHDLSNGAM
     NMLEVFVSSL EEFQPDLVVL SGLHMMEGQS KELQRKRLLE VVTSISDIPT GIPVHLELAS
     MTNRELMSSI VHQQVFPAVT SLGLNEQELL FLTQSASGPH SSLSSWNGVP DVGMVSDILF
     WILKEHGRSK SRASDLTRIH FHTLVYHILA TVDGHWANQL AAVAAGARVA GTQACATETI
     DTSRVSLRAP QEFMTSHSEA GSRIVLNPNK PVVEWHREGI SFHFTPVLVC KDPIRTVGLG
     DAISAEGLFY SEVHPHY
//