{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q9A1S2","secondaryAccessions":["Q491J0"],"uniProtkbId":"PILIN_STRP1","entryAudit":{"firstPublicDate":"2008-04-29","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2001-06-01","entryVersion":124,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Streptococcus pyogenes serotype M1","taxonId":301447,"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Lactobacillales","Streptococcaceae","Streptococcus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Pilin"}},"alternativeNames":[{"fullName":{"value":"Lancefield T antigen"}},{"fullName":{"value":"Pilus backbone structural protein"}}],"flag":"Precursor"},"genes":[{"orderedLocusNames":[{"value":"SPy_0128"},{"value":"M5005_Spy0109"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17501921"}],"value":"Major component of the pilus. A stack of the pilin subunits, joined by intermolecular isopeptide bonds, forms the pilus. The pilus is required for bacterial adhesion to host cells, for bacterial aggregation, and for biofilm formation"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18063798"}],"value":"Forms columns of about 3-nanometers in diameter of head-to-tail-assembled molecules"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"value":"Attached to the cell wall by a peptidoglycan anchor"}]},"subcellularLocations":[{"location":{"value":"Secreted, cell wall","id":"SL-0041"},"topology":{"value":"Peptidoglycan-anchor","id":"SL-9900"}},{"location":{"value":"Fimbrium","id":"SL-0113"}}]},{"texts":[{"value":"Proteolytically processed and assembled in pili through a transpeptidation reaction catalyzed by the sortase C1. The last pilin subunit is cross-linked to the peptidoglycan"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the Streptococcus pilin family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":23,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Chain","location":{"start":{"value":24,"modifier":"EXACT"},"end":{"value":311,"modifier":"EXACT"}},"description":"Pilin","featureId":"PRO_0000331363"},{"type":"Propeptide","location":{"start":{"value":312,"modifier":"EXACT"},"end":{"value":340,"modifier":"EXACT"}},"description":"Removed by sortase C1","evidences":[{"evidenceCode":"ECO:0000305"}],"featureId":"PRO_0000331364"},{"type":"Motif","location":{"start":{"value":308,"modifier":"EXACT"},"end":{"value":312,"modifier":"EXACT"}},"description":"EVPTG sorting signal","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Site","location":{"start":{"value":117,"modifier":"EXACT"},"end":{"value":117,"modifier":"EXACT"}},"description":"Autocatalyzes isopeptide 36-168 formation"},{"type":"Site","location":{"start":{"value":258,"modifier":"EXACT"},"end":{"value":258,"modifier":"EXACT"}},"description":"Autocatalyzes isopeptide 179-303 formation"},{"type":"Modified residue","location":{"start":{"value":311,"modifier":"EXACT"},"end":{"value":311,"modifier":"EXACT"}},"description":"Pentaglycyl murein peptidoglycan amidated threonine; alternate","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Cross-link","location":{"start":{"value":36,"modifier":"EXACT"},"end":{"value":168,"modifier":"EXACT"}},"description":"Isoaspartyl lysine isopeptide (Lys-Asn)"},{"type":"Cross-link","location":{"start":{"value":161,"modifier":"EXACT"},"end":{"value":161,"modifier":"EXACT"}},"description":"Threonyl lysine isopeptide (Lys-Thr) (interchain with T-311)"},{"type":"Cross-link","location":{"start":{"value":179,"modifier":"EXACT"},"end":{"value":303,"modifier":"EXACT"}},"description":"Isoaspartyl lysine isopeptide (Lys-Asn)"},{"type":"Cross-link","location":{"start":{"value":311,"modifier":"EXACT"},"end":{"value":311,"modifier":"EXACT"}},"description":"Threonyl lysine isopeptide (Thr-Lys) (interchain with K-161); alternate"},{"type":"Mutagenesis","location":{"start":{"value":117,"modifier":"EXACT"},"end":{"value":117,"modifier":"EXACT"}},"description":"Increased susceptibility to proteolysis.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18063798"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":258,"modifier":"EXACT"},"end":{"value":258,"modifier":"EXACT"}},"description":"Increased susceptibility to proteolysis.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18063798"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["A"]}},{"type":"Helix","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":27,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3B2M"}]},{"type":"Beta strand","location":{"start":{"value":31,"modifier":"EXACT"},"end":{"value":42,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GLD"}]},{"type":"Helix","location":{"start":{"value":45,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GLD"}]},{"type":"Beta strand","location":{"start":{"value":49,"modifier":"EXACT"},"end":{"value":58,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GLD"}]},{"type":"Beta strand","location":{"start":{"value":65,"modifier":"EXACT"},"end":{"value":70,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GLD"}]},{"type":"Helix","location":{"start":{"value":71,"modifier":"EXACT"},"end":{"value":74,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GLD"}]},{"type":"Beta strand","location":{"start":{"value":78,"modifier":"EXACT"},"end":{"value":83,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GLD"}]},{"type":"Helix","location":{"start":{"value":85,"modifier":"EXACT"},"end":{"value":87,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GLD"}]},{"type":"Beta strand","location":{"start":{"value":93,"modifier":"EXACT"},"end":{"value":99,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GLD"}]},{"type":"Beta strand","location":{"start":{"value":107,"modifier":"EXACT"},"end":{"value":117,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GLD"}]},{"type":"Beta strand","location":{"start":{"value":132,"modifier":"EXACT"},"end":{"value":142,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GLD"}]},{"type":"Turn","location":{"start":{"value":143,"modifier":"EXACT"},"end":{"value":146,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GLD"}]},{"type":"Beta strand","location":{"start":{"value":147,"modifier":"EXACT"},"end":{"value":157,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GLD"}]},{"type":"Beta strand","location":{"start":{"value":164,"modifier":"EXACT"},"end":{"value":170,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GLD"}]},{"type":"Beta strand","location":{"start":{"value":172,"modifier":"EXACT"},"end":{"value":183,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GLD"}]},{"type":"Beta strand","location":{"start":{"value":192,"modifier":"EXACT"},"end":{"value":198,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GLD"}]},{"type":"Beta strand","location":{"start":{"value":202,"modifier":"EXACT"},"end":{"value":204,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GLE"}]},{"type":"Beta strand","location":{"start":{"value":209,"modifier":"EXACT"},"end":{"value":215,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GLD"}]},{"type":"Beta strand","location":{"start":{"value":217,"modifier":"EXACT"},"end":{"value":219,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GLD"}]},{"type":"Helix","location":{"start":{"value":220,"modifier":"EXACT"},"end":{"value":222,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GLD"}]},{"type":"Beta strand","location":{"start":{"value":223,"modifier":"EXACT"},"end":{"value":228,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GLD"}]},{"type":"Beta strand","location":{"start":{"value":233,"modifier":"EXACT"},"end":{"value":237,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GLD"}]},{"type":"Beta strand","location":{"start":{"value":242,"modifier":"EXACT"},"end":{"value":249,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GLD"}]},{"type":"Beta strand","location":{"start":{"value":253,"modifier":"EXACT"},"end":{"value":258,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GLD"}]},{"type":"Helix","location":{"start":{"value":262,"modifier":"EXACT"},"end":{"value":264,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GLD"}]},{"type":"Beta strand","location":{"start":{"value":267,"modifier":"EXACT"},"end":{"value":274,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GLD"}]},{"type":"Beta strand","location":{"start":{"value":280,"modifier":"EXACT"},"end":{"value":286,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GLD"}]},{"type":"Beta strand","location":{"start":{"value":297,"modifier":"EXACT"},"end":{"value":304,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GLD"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0134","category":"Cellular component","name":"Cell wall"},{"id":"KW-0281","category":"Cellular component","name":"Fimbrium"},{"id":"KW-1017","category":"PTM","name":"Isopeptide bond"},{"id":"KW-0572","category":"PTM","name":"Peptidoglycan-anchor"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"id":"KW-0732","category":"Domain","name":"Signal"}],"references":[{"referenceNumber":1,"citation":{"id":"11296296","citationType":"journal article","authors":["Ferretti J.J.","McShan W.M.","Ajdic D.J.","Savic D.J.","Savic G.","Lyon K.","Primeaux C.","Sezate S.","Suvorov A.N.","Kenton S.","Lai H.S.","Lin S.P.","Qian Y.","Jia H.G.","Najar F.Z.","Ren Q.","Zhu H.","Song L.","White J.","Yuan X.","Clifton S.W.","Roe B.A.","McLaughlin R.E."],"citationCrossReferences":[{"database":"PubMed","id":"11296296"},{"database":"DOI","id":"10.1073/pnas.071559398"}],"title":"Complete genome sequence of an M1 strain of Streptococcus pyogenes.","publicationDate":"2001","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"4658","lastPage":"4663","volume":"98"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 700294 / SF370 / Serotype M1","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"16088826","citationType":"journal article","authors":["Sumby P.","Porcella S.F.","Madrigal A.G.","Barbian K.D.","Virtaneva K.","Ricklefs S.M.","Sturdevant D.E.","Graham M.R.","Vuopio-Varkila J.","Hoe N.P.","Musser J.M."],"citationCrossReferences":[{"database":"PubMed","id":"16088826"},{"database":"DOI","id":"10.1086/432514"}],"title":"Evolutionary origin and emergence of a highly successful clone of serotype M1 group A Streptococcus involved multiple horizontal gene transfer events.","publicationDate":"2005","journal":"J. Infect. Dis.","firstPage":"771","lastPage":"782","volume":"192"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC BAA-947 / MGAS5005 / Serotype M1","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"17501921","citationType":"journal article","authors":["Manetti A.G.","Zingaretti C.","Falugi F.","Capo S.","Bombaci M.","Bagnoli F.","Gambellini G.","Bensi G.","Mora M.","Edwards A.M.","Musser J.M.","Graviss E.A.","Telford J.L.","Grandi G.","Margarit I."],"citationCrossReferences":[{"database":"PubMed","id":"17501921"},{"database":"DOI","id":"10.1111/j.1365-2958.2007.05704.x"}],"title":"Streptococcus pyogenes pili promote pharyngeal cell adhesion and biofilm formation.","publicationDate":"2007","journal":"Mol. Microbiol.","firstPage":"968","lastPage":"983","volume":"64"},"referencePositions":["FUNCTION"]},{"referenceNumber":4,"citation":{"id":"18063798","citationType":"journal article","authors":["Kang H.J.","Coulibaly F.","Clow F.","Proft T.","Baker E.N."],"citationCrossReferences":[{"database":"PubMed","id":"18063798"},{"database":"DOI","id":"10.1126/science.1145806"}],"title":"Stabilizing isopeptide bonds revealed in Gram-positive bacterial pilus structure.","publicationDate":"2007","journal":"Science","firstPage":"1625","lastPage":"1628","volume":"318"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (2.22 ANGSTROMS)","SUBUNIT","CROSS-LINKS","MUTAGENESIS OF GLU-117 AND GLU-258"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AE004092","properties":[{"key":"ProteinId","value":"AAK33238.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP000017","properties":[{"key":"ProteinId","value":"AAZ50728.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PDB","id":"3B2M","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.22 A"},{"key":"Chains","value":"A/B/C=18-307"}]},{"database":"PDB","id":"3GLD","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.03 A"},{"key":"Chains","value":"A/B/C=18-308"}]},{"database":"PDB","id":"3GLE","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.19 A"},{"key":"Chains","value":"A/B/C=18-308"}]},{"database":"PDBsum","id":"3B2M","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"3GLD","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"3GLE","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"Q9A1S2","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q9A1S2","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"spy:SPy_0128","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"spz:M5005_Spy0109","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|160490.10.peg.110","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"HOGENOM","id":"CLU_787371_0_0_9","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"WTVDVYV","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"EvolutionaryTrace","id":"Q9A1S2","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000750","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0009289","properties":[{"key":"GoTerm","value":"C:pilus"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"Gene3D","id":"2.60.40.3050","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.60.40.1140","properties":[{"key":"EntryName","value":"Collagen-binding surface protein Cna, B-type domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR055382","properties":[{"key":"EntryName","value":"DUF7601"}]},{"database":"InterPro","id":"IPR017503","properties":[{"key":"EntryName","value":"Sortase_SrtB_sig_QVPTGV"}]},{"database":"InterPro","id":"IPR022464","properties":[{"key":"EntryName","value":"Strep_pil_isopept_link"}]},{"database":"InterPro","id":"IPR038174","properties":[{"key":"EntryName","value":"Strep_pil_link_sf"}]},{"database":"NCBIfam","id":"TIGR03065","properties":[{"key":"EntryName","value":"srtB_sig_QVPTGV"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR03786","properties":[{"key":"EntryName","value":"strep_pil_rpt"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF24547","properties":[{"key":"EntryName","value":"DUF7601"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF12892","properties":[{"key":"EntryName","value":"FctA"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKLRHLLLTGAALTSFAATTVHGETVVNGAKLTVTKNLDLVNSNALIPNTDFTFKIEPDTTVNEDGNKFKGVALNTPMTKVTYTNSDKGGSNTKTAEFDFSEVTFEKPGVYYYKVTEEKIDKVPGVSYDTTSYTVQVHVLWNEEQQKPVATYIVGYKEGSKVPIQFKNSLDSTTLTVKKKVSGTGGDRSKDFNFGLTLKANQYYKASEKVMIEKTTKGGQAPVQTEASIDQLYHFTLKDGESIKVTNLPVGVDYVVTEDDYKSEKYTTNVEVSPQDGAVKNIAGNSTEQETSTDKDMTITFTNKKDFEVPTGVAMTVAPYIALGIVAVGGALYFVKKKNA","length":340,"molWeight":37188,"crc64":"9EB0C99CBF42161B","md5":"12D8A2D99AB31E7166B123B89CA8D729"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"PTM":1,"SIMILARITY":1},"countByFeatureType":{"Signal":1,"Chain":1,"Propeptide":1,"Motif":1,"Site":2,"Modified residue":1,"Cross-link":4,"Mutagenesis":2,"Helix":6,"Beta strand":21,"Turn":1},"uniParcId":"UPI00000C7BDC"}}