{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q97Y91","uniProtkbId":"CSA2B_SACS2","entryAudit":{"firstPublicDate":"2012-06-13","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2001-10-01","entryVersion":103,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)","commonName":"Sulfolobus solfataricus","taxonId":273057,"lineage":["Archaea","Thermoproteota","Thermoprotei","Sulfolobales","Sulfolobaceae","Saccharolobus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"CRISPR-associated aCascade subunit Cas7/Csa2 2"}},"alternativeNames":[{"fullName":{"value":"CRISPR-associated aCascade subunit Cas7/Csa2, subtype I-A/Apern 2"}}]},"genes":[{"geneName":{"value":"csa2b"},"synonyms":[{"value":"cas7b"}],"orderedLocusNames":[{"value":"SSO1442"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21507944"}],"value":"Part of the aCascade ribonucleoprotein complex, minimally composed of Csa2 and Cas5a, which binds crRNA. Other possible components of aCascade in strain P1 are Cas6b (SSO1437) and Csa5 (SSO1443), while SSO1399, Cas5b (SSO1400) and SSO1401 have sometimes been seen weakly associated. Csa2 is probably the major RNA-binding subunit. The Csa2-Cas5a-crRNA complex also binds target DNA homologous to crRNA, probably forming an R-loop. Purified aCascade forms a filament about 6 nm in width"}],"commentType":"SUBUNIT"},{"texts":[{"value":"Crystallography and cloning were done with strain P2, while interaction experiments were done with genes cloned from strain P2 but over-expressed in P1. The aCascade complex was purified from strain P1"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the CRISPR-associated protein Cas7/Cst2/DevR family. Subtype I-a/Apern subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":321,"modifier":"EXACT"}},"description":"CRISPR-associated aCascade subunit Cas7/Csa2 2","featureId":"PRO_0000417885"},{"type":"Mutagenesis","location":{"start":{"value":160,"modifier":"EXACT"},"end":{"value":160,"modifier":"EXACT"}},"description":"Significantly reduced affinity for crRNA.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21507944"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["A"]}},{"type":"Beta strand","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":11,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3PS0"}]},{"type":"Helix","location":{"start":{"value":12,"modifier":"EXACT"},"end":{"value":15,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3PS0"}]},{"type":"Beta strand","location":{"start":{"value":16,"modifier":"EXACT"},"end":{"value":21,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9CP2"}]},{"type":"Beta strand","location":{"start":{"value":24,"modifier":"EXACT"},"end":{"value":26,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3PS0"}]},{"type":"Beta strand","location":{"start":{"value":28,"modifier":"EXACT"},"end":{"value":36,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3PS0"}]},{"type":"Beta strand","location":{"start":{"value":39,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3PS0"}]},{"type":"Helix","location":{"start":{"value":50,"modifier":"EXACT"},"end":{"value":68,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3PS0"}]},{"type":"Helix","location":{"start":{"value":76,"modifier":"EXACT"},"end":{"value":78,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3PS0"}]},{"type":"Turn","location":{"start":{"value":81,"modifier":"EXACT"},"end":{"value":84,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9CP2"}]},{"type":"Helix","location":{"start":{"value":87,"modifier":"EXACT"},"end":{"value":93,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3PS0"}]},{"type":"Helix","location":{"start":{"value":96,"modifier":"EXACT"},"end":{"value":98,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3PS0"}]},{"type":"Helix","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":103,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3PS0"}]},{"type":"Helix","location":{"start":{"value":104,"modifier":"EXACT"},"end":{"value":114,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3PS0"}]},{"type":"Helix","location":{"start":{"value":116,"modifier":"EXACT"},"end":{"value":120,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3PS0"}]},{"type":"Beta strand","location":{"start":{"value":123,"modifier":"EXACT"},"end":{"value":125,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3PS0"}]},{"type":"Beta strand","location":{"start":{"value":127,"modifier":"EXACT"},"end":{"value":129,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3PS0"}]},{"type":"Beta strand","location":{"start":{"value":131,"modifier":"EXACT"},"end":{"value":133,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3PS0"}]},{"type":"Beta strand","location":{"start":{"value":136,"modifier":"EXACT"},"end":{"value":140,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3PS0"}]},{"type":"Beta strand","location":{"start":{"value":142,"modifier":"EXACT"},"end":{"value":144,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3PS0"}]},{"type":"Beta strand","location":{"start":{"value":148,"modifier":"EXACT"},"end":{"value":150,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9CP2"}]},{"type":"Helix","location":{"start":{"value":151,"modifier":"EXACT"},"end":{"value":158,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3PS0"}]},{"type":"Beta strand","location":{"start":{"value":180,"modifier":"EXACT"},"end":{"value":190,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3PS0"}]},{"type":"Helix","location":{"start":{"value":192,"modifier":"EXACT"},"end":{"value":194,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3PS0"}]},{"type":"Helix","location":{"start":{"value":207,"modifier":"EXACT"},"end":{"value":224,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3PS0"}]},{"type":"Helix","location":{"start":{"value":226,"modifier":"EXACT"},"end":{"value":230,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3PS0"}]},{"type":"Turn","location":{"start":{"value":238,"modifier":"EXACT"},"end":{"value":240,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9CP2"}]},{"type":"Beta strand","location":{"start":{"value":245,"modifier":"EXACT"},"end":{"value":257,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3PS0"}]},{"type":"Helix","location":{"start":{"value":261,"modifier":"EXACT"},"end":{"value":263,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3PS0"}]},{"type":"Beta strand","location":{"start":{"value":264,"modifier":"EXACT"},"end":{"value":266,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3PS0"}]},{"type":"Helix","location":{"start":{"value":269,"modifier":"EXACT"},"end":{"value":283,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3PS0"}]},{"type":"Beta strand","location":{"start":{"value":289,"modifier":"EXACT"},"end":{"value":297,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3PS0"}]},{"type":"Helix","location":{"start":{"value":311,"modifier":"EXACT"},"end":{"value":319,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3PS0"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0051","category":"Biological process","name":"Antiviral defense"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0694","category":"Molecular function","name":"RNA-binding"}],"references":[{"referenceNumber":1,"citation":{"id":"11427726","citationType":"journal article","authors":["She Q.","Singh R.K.","Confalonieri F.","Zivanovic Y.","Allard G.","Awayez M.J.","Chan-Weiher C.C.-Y.","Clausen I.G.","Curtis B.A.","De Moors A.","Erauso G.","Fletcher C.","Gordon P.M.K.","Heikamp-de Jong I.","Jeffries A.C.","Kozera C.J.","Medina N.","Peng X.","Thi-Ngoc H.P.","Redder P.","Schenk M.E.","Theriault C.","Tolstrup N.","Charlebois R.L.","Doolittle W.F.","Duguet M.","Gaasterland T.","Garrett R.A.","Ragan M.A.","Sensen C.W.","Van der Oost J."],"citationCrossReferences":[{"database":"PubMed","id":"11427726"},{"database":"DOI","id":"10.1073/pnas.141222098"}],"title":"The complete genome of the crenarchaeon Sulfolobus solfataricus P2.","publicationDate":"2001","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"7835","lastPage":"7840","volume":"98"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 35092 / DSM 1617 / JCM 11322 / P2","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"21507944","citationType":"journal article","authors":["Lintner N.G.","Kerou M.","Brumfield S.K.","Graham S.","Liu H.","Naismith J.H.","Sdano M.","Peng N.","She Q.","Copie V.","Young M.J.","White M.F.","Lawrence C.M."],"citationCrossReferences":[{"database":"PubMed","id":"21507944"},{"database":"DOI","id":"10.1074/jbc.m111.238485"}],"title":"Structural and functional characterization of an archaeal clustered regularly interspaced short palindromic repeat (CRISPR)-associated complex for antiviral defense (CASCADE).","publicationDate":"2011","journal":"J. Biol. Chem.","firstPage":"21643","lastPage":"21656","volume":"286"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS)","SUBUNIT","RNA-BINDING","INTERACTION WITH CAS5E","MUTAGENESIS OF HIS-160"],"referenceComments":[{"value":"ATCC 35091 / DSM 1616 / JCM 8930 / NBRC 15331 / P1","type":"STRAIN"},{"value":"ATCC 35092 / DSM 1617 / JCM 11322 / P2","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AE006641","properties":[{"key":"ProteinId","value":"AAK41675.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"D90302","properties":[{"key":"EntryName","value":"D90302"}]},{"database":"RefSeq","id":"WP_010923407.1","properties":[{"key":"NucleotideSequenceId","value":"NC_002754.1"}]},{"database":"PDB","id":"3PS0","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.00 A"},{"key":"Chains","value":"A/B/C/D=1-321"}]},{"database":"PDB","id":"9CP1","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.97 A"},{"key":"Chains","value":"A/B/C/D/E/F=1-321"}]},{"database":"PDB","id":"9CP2","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.94 A"},{"key":"Chains","value":"A/B/C/D=1-321"}]},{"database":"PDB","id":"9CP3","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.94 A"},{"key":"Chains","value":"A/B/C/D/E=1-321"}]},{"database":"PDB","id":"9CRO","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.50 A"},{"key":"Chains","value":"A/B/C/D/E/F/G=1-321"}]},{"database":"PDB","id":"9CRP","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.20 A"},{"key":"Chains","value":"A/B/C/D/E/F=1-321"}]},{"database":"PDB","id":"9CRQ","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.07 A"},{"key":"Chains","value":"A/B/C/D/E=1-321"}]},{"database":"PDBsum","id":"3PS0","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9CP1","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9CP2","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9CP3","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9CRO","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9CRP","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9CRQ","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"Q97Y91","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-45796","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-45797","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-45798","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-45873","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-45874","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-45875","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q97Y91","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"273057.SSO1442","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"273057-SSO1442","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAK41675","properties":[{"key":"ProteinId","value":"AAK41675"},{"key":"GeneId","value":"SSO1442"}]},{"database":"GeneID","id":"1454454","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"sso:SSO1442","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|273057.12.peg.1471","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"eggNOG","id":"arCOG03617","properties":[{"key":"ToxonomicScope","value":"Archaea"}]},{"database":"HOGENOM","id":"CLU_054331_0_0_2","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q97Y91","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q97Y91","properties":[{"key":"Description","value":"-"}]},{"database":"EvolutionaryTrace","id":"Q97Y91","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000001974","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"CDD","id":"cd09685","properties":[{"key":"EntryName","value":"Cas7_I-A"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR002764","properties":[{"key":"EntryName","value":"Cas7/Cst2/DevR_sub_I-a/Apern"}]},{"database":"InterPro","id":"IPR010154","properties":[{"key":"EntryName","value":"CRISPR-assoc_Cas7/Cst2/DevR"}]},{"database":"InterPro","id":"IPR052681","properties":[{"key":"EntryName","value":"CRISPR-Cas7/Cst2/DevR"}]},{"database":"NCBIfam","id":"TIGR01875","properties":[{"key":"EntryName","value":"cas_MJ0381"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR02583","properties":[{"key":"EntryName","value":"DevR_archaea"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR37459","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR37459:SF1","properties":[{"key":"EntryName","value":"CRISPR-ASSOCIATED PROTEIN CAS7_CST2_DEVR"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01905","properties":[{"key":"EntryName","value":"DevR"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MISGSVRFLVNLESLNGVESIGNLTKHRTAPVVLKTSTGYLVRYVPVISGEALAHAYQASLVDIAKKEGLPVGSLSSQYEFIKFSTDEALKIEGIKEPKDYNDARRFEVEVMLKDVIADVGGFMYAGGAPVRRTSRIKLGYMIPALRGDEIPAQLEAQFHVRFSNKPVSGSQAIFNVEVSSALYTFSFELDEDLIAVPSTFGEKVKGEEELERQKAKRVKSAIKALYSLLSGNFGGKRSRFLPSMKLMSLVVTKTDFPFMPEPAHDDDYIKTTIMRLGKAKGVLNGNLAKAYVINNEGIEVGEGVTVLSTVEDLVVKLEEE","length":321,"molWeight":35265,"crc64":"0B5959530AC2709A","md5":"8FBD8EEB22364A93997DFFE520F94F73"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Mutagenesis":1,"Beta strand":15,"Helix":15,"Turn":2},"uniParcId":"UPI000006446C"}}