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Required for chromosome segregation, the assembly of the dynein-dynactin and mdf-1-mdf-2 complexes onto kinetochores and spindle pole separation (PubMed:18765790). Its function related to the spindle assembly machinery and kinetochore-microtubule attachments likely depends on its association in the mitotic RZZ complex (PubMed:18765790). The RZZ complex recruits the spindly-like protein spdl-1 to kinetochores (PubMed:18765790). To prevent irregular chromosome segregation, the complex also inhibits the attachment of the kinetochore-associated NDC80 complex to microtubules (PubMed:24231804). The recruitment of spdl-1 to kinetochores relieves this inhibition (PubMed:24231804). Required for embryonic development (PubMed:18765790, PubMed:19109417)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18765790"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24231804"}],"value":"Component of the RZZ complex composed of rod-1, czw-1 and zwl-1 (PubMed:18765790). Interacts with the spindly-like protein spdl-1 (PubMed:18765790). Interacts with NDC80 complex component ndc-80 (PubMed:24231804)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18765790"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20729837"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"18765790"}],"value":"Localizes to the kinetochore during nuclear envelope breakdown and remains there until the metaphase-anaphase transition (PubMed:18765790). Localizes to kinetochores, spindle-associated rod-shaped structures and near the cell cortex in a knl-1-dependent manner in anaphase (PubMed:18765790, PubMed:20729837)"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20729837"}],"value":"Cytoplasm, cell cortex","id":"SL-0138"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18765790"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19109417"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20729837"}],"value":"Chromosome, centromere, kinetochore","id":"SL-0149"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Cytoplasm, cytoskeleton, spindle","id":"SL-0251"}}]},{"commentType":"ALTERNATIVE PRODUCTS","events":["Alternative splicing"],"isoforms":[{"name":{"evidences":[{"evidenceCode":"ECO:0000312","source":"WormBase","id":"Y39G10AR.2a"}],"value":"a"},"isoformIds":["Q95XP9-1"],"isoformSequenceStatus":"Displayed"},{"name":{"evidences":[{"evidenceCode":"ECO:0000312","source":"WormBase","id":"Y39G10AR.2b"}],"value":"b"},"isoformIds":["Q95XP9-2"],"sequenceIds":["VSP_057650"],"isoformSequenceStatus":"Described"},{"name":{"evidences":[{"evidenceCode":"ECO:0000312","source":"WormBase","id":"Y39G10AR.2c"}],"value":"c"},"isoformIds":["Q95XP9-3"],"sequenceIds":["VSP_057649"],"isoformSequenceStatus":"Described"},{"name":{"evidences":[{"evidenceCode":"ECO:0000312","source":"WormBase","id":"Y39G10AR.2d"}],"value":"d"},"isoformIds":["Q95XP9-4"],"sequenceIds":["VSP_057649","VSP_057650"],"isoformSequenceStatus":"Described"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18765790"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19109417"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20729837"}],"value":"RNAi-mediated knockdown results in embryonic lethality (PubMed:18765790, PubMed:19109417). RNAi-mediated knockdown results in defective chromosome segregation, premature spindle pole separation and incorrectly attached kinetochores (PubMed:18765790). In addition, knl-1 localization to the spindle-associated rod-shaped structures but not to kinetochores is lost during anaphase of meiosis I (PubMed:20729837). RNAi-mediated knockdown also results in abolished targeting of the spindly-like protein spdl-1 to the kinetochore (PubMed:18765790)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the ZWILCH family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":630,"modifier":"EXACT"}},"description":"Protein zwilch homolog","featureId":"PRO_0000314807"},{"type":"Alternative sequence","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":321,"modifier":"EXACT"}},"description":"in isoform c and isoform d","evidences":[{"evidenceCode":"ECO:0000305"}],"featureId":"VSP_057649","alternativeSequence":{}},{"type":"Alternative sequence","location":{"start":{"value":354,"modifier":"EXACT"},"end":{"value":356,"modifier":"EXACT"}},"description":"in isoform b and isoform d","evidences":[{"evidenceCode":"ECO:0000305"}],"featureId":"VSP_057650","alternativeSequence":{"originalSequence":"NFR","alternativeSequences":["K"]}}],"keywords":[{"id":"KW-0025","category":"Coding sequence diversity","name":"Alternative splicing"},{"id":"KW-0131","category":"Biological process","name":"Cell cycle"},{"id":"KW-0132","category":"Biological process","name":"Cell division"},{"id":"KW-0137","category":"Cellular component","name":"Centromere"},{"id":"KW-0158","category":"Cellular component","name":"Chromosome"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0206","category":"Cellular component","name":"Cytoskeleton"},{"id":"KW-0217","category":"Molecular function","name":"Developmental protein"},{"id":"KW-0995","category":"Cellular component","name":"Kinetochore"},{"id":"KW-0469","category":"Biological process","name":"Meiosis"},{"id":"KW-0498","category":"Biological process","name":"Mitosis"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"9851916","citationType":"journal article","authoringGroup":["The C. elegans sequencing consortium"],"citationCrossReferences":[{"database":"PubMed","id":"9851916"},{"database":"DOI","id":"10.1126/science.282.5396.2012"}],"title":"Genome sequence of the nematode C. elegans: a platform for investigating biology.","publicationDate":"1998","journal":"Science","firstPage":"2012","lastPage":"2018","volume":"282"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"Bristol N2","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"18765790","citationType":"journal article","authors":["Gassmann R.","Essex A.","Hu J.-S.","Maddox P.S.","Motegi F.","Sugimoto A.","O'Rourke S.M.","Bowerman B.","McLeod I.","Yates J.R. 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