{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q922R1","secondaryAccessions":["Q6PER2"],"uniProtkbId":"PHAF1_MOUSE","entryAudit":{"firstPublicDate":"2002-01-23","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2011-07-27","entryVersion":136,"sequenceVersion":2},"annotationScore":2.0,"organism":{"scientificName":"Mus musculus","commonName":"Mouse","taxonId":10090,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Glires","Rodentia","Myomorpha","Muroidea","Muridae","Murinae","Mus","Mus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000312","source":"MGI","id":"MGI:2443049"}],"value":"Phagosome assembly factor 1"}}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000312","source":"MGI","id":"MGI:2443049"}],"value":"Phaf1"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9BSU1"}],"value":"Plays a regulatory role in autophagic activity. In complex with BCAS3, associates with the autophagosome formation site during both non-selective and selective autophagy"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9BSU1"}],"value":"Interacts with BCAS3; the interaction is requrired for the association with the phagophore"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9BSU1"}],"value":"The BCAS3:PHAF1 complex is recruited to the preautophagosomal structures adjacent to the damaged mitochondria upon mitophagy in a PRKN-PINK1 dependent manner"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9BSU1"}],"value":"Cytoplasm","id":"SL-0086"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9BSU1"}],"value":"Preautophagosomal structure","id":"SL-0220"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the PHAF1 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":422,"modifier":"EXACT"}},"description":"Phagosome assembly factor 1","featureId":"PRO_0000221084"},{"type":"Sequence conflict","location":{"start":{"value":356,"modifier":"EXACT"},"end":{"value":356,"modifier":"EXACT"}},"description":"in Ref. 3; AAH06874","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["D"]}}],"keywords":[{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"16141072","citationType":"journal article","authors":["Carninci P.","Kasukawa T.","Katayama S.","Gough J.","Frith M.C.","Maeda N.","Oyama R.","Ravasi T.","Lenhard B.","Wells C.","Kodzius R.","Shimokawa K.","Bajic V.B.","Brenner S.E.","Batalov S.","Forrest A.R.","Zavolan M.","Davis M.J.","Wilming L.G.","Aidinis V.","Allen J.E.","Ambesi-Impiombato A.","Apweiler R.","Aturaliya R.N.","Bailey T.L.","Bansal M.","Baxter L.","Beisel K.W.","Bersano T.","Bono H.","Chalk A.M.","Chiu K.P.","Choudhary V.","Christoffels A.","Clutterbuck D.R.","Crowe M.L.","Dalla E.","Dalrymple B.P.","de Bono B.","Della Gatta G.","di Bernardo D.","Down T.","Engstrom P.","Fagiolini M.","Faulkner G.","Fletcher C.F.","Fukushima T.","Furuno M.","Futaki S.","Gariboldi M.","Georgii-Hemming P.","Gingeras T.R.","Gojobori T.","Green R.E.","Gustincich S.","Harbers M.","Hayashi Y.","Hensch T.K.","Hirokawa N.","Hill D.","Huminiecki L.","Iacono M.","Ikeo K.","Iwama A.","Ishikawa T.","Jakt M.","Kanapin A.","Katoh M.","Kawasawa Y.","Kelso J.","Kitamura H.","Kitano H.","Kollias G.","Krishnan S.P.","Kruger A.","Kummerfeld S.K.","Kurochkin I.V.","Lareau L.F.","Lazarevic D.","Lipovich L.","Liu J.","Liuni S.","McWilliam S.","Madan Babu M.","Madera M.","Marchionni L.","Matsuda H.","Matsuzawa S.","Miki H.","Mignone F.","Miyake S.","Morris K.","Mottagui-Tabar S.","Mulder N.","Nakano N.","Nakauchi H.","Ng P.","Nilsson R.","Nishiguchi S.","Nishikawa S.","Nori F.","Ohara O.","Okazaki Y.","Orlando V.","Pang K.C.","Pavan W.J.","Pavesi G.","Pesole G.","Petrovsky N.","Piazza S.","Reed J.","Reid J.F.","Ring B.Z.","Ringwald M.","Rost B.","Ruan Y.","Salzberg S.L.","Sandelin A.","Schneider C.","Schoenbach C.","Sekiguchi K.","Semple C.A.","Seno S.","Sessa L.","Sheng Y.","Shibata Y.","Shimada H.","Shimada K.","Silva D.","Sinclair B.","Sperling S.","Stupka E.","Sugiura K.","Sultana R.","Takenaka Y.","Taki K.","Tammoja K.","Tan S.L.","Tang S.","Taylor M.S.","Tegner J.","Teichmann S.A.","Ueda H.R.","van Nimwegen E.","Verardo R.","Wei C.L.","Yagi K.","Yamanishi H.","Zabarovsky E.","Zhu S.","Zimmer A.","Hide W.","Bult C.","Grimmond S.M.","Teasdale R.D.","Liu E.T.","Brusic V.","Quackenbush J.","Wahlestedt C.","Mattick J.S.","Hume D.A.","Kai C.","Sasaki D.","Tomaru Y.","Fukuda S.","Kanamori-Katayama M.","Suzuki M.","Aoki J.","Arakawa T.","Iida J.","Imamura K.","Itoh M.","Kato T.","Kawaji H.","Kawagashira N.","Kawashima T.","Kojima M.","Kondo S.","Konno H.","Nakano K.","Ninomiya N.","Nishio T.","Okada M.","Plessy C.","Shibata K.","Shiraki T.","Suzuki S.","Tagami M.","Waki K.","Watahiki A.","Okamura-Oho Y.","Suzuki H.","Kawai J.","Hayashizaki Y."],"citationCrossReferences":[{"database":"PubMed","id":"16141072"},{"database":"DOI","id":"10.1126/science.1112014"}],"title":"The transcriptional landscape of the mammalian genome.","publicationDate":"2005","journal":"Science","firstPage":"1559","lastPage":"1563","volume":"309"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"NOD","type":"STRAIN"},{"value":"Spleen","type":"TISSUE"}]},{"referenceNumber":2,"citation":{"id":"CI-70VOHH7BF94TO","citationType":"submission","authors":["Mural R.J.","Adams M.D.","Myers E.W.","Smith H.O.","Venter J.C."],"publicationDate":"JUL-2005","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"]},{"referenceNumber":3,"citation":{"id":"15489334","citationType":"journal article","authoringGroup":["The MGC Project Team"],"citationCrossReferences":[{"database":"PubMed","id":"15489334"},{"database":"DOI","id":"10.1101/gr.2596504"}],"title":"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).","publicationDate":"2004","journal":"Genome Res.","firstPage":"2121","lastPage":"2127","volume":"14"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"FVB/N","type":"STRAIN"},{"value":"Liver","type":"TISSUE"}]},{"referenceNumber":4,"citation":{"id":"21183079","citationType":"journal article","authors":["Huttlin E.L.","Jedrychowski M.P.","Elias J.E.","Goswami T.","Rad R.","Beausoleil S.A.","Villen J.","Haas W.","Sowa M.E.","Gygi S.P."],"citationCrossReferences":[{"database":"PubMed","id":"21183079"},{"database":"DOI","id":"10.1016/j.cell.2010.12.001"}],"title":"A tissue-specific atlas of mouse protein phosphorylation and expression.","publicationDate":"2010","journal":"Cell","firstPage":"1174","lastPage":"1189","volume":"143"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"Brain","type":"TISSUE"},{"value":"Spleen","type":"TISSUE"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AK156120","properties":[{"key":"ProteinId","value":"BAE33592.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"CH466525","properties":[{"key":"ProteinId","value":"EDL11253.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"BC006874","properties":[{"key":"ProteinId","value":"AAH06874.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC057919","properties":[{"key":"ProteinId","value":"AAH57919.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"CCDS","id":"CCDS22593.1","properties":[{"key":"Description","value":"-"}]},{"database":"RefSeq","id":"NP_663579.2","properties":[{"key":"NucleotideSequenceId","value":"NM_145604.3"}]},{"database":"AlphaFoldDB","id":"Q922R1","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"231557","properties":[{"key":"Interactions","value":"1"}]},{"database":"FunCoup","id":"Q922R1","properties":[{"key":"Number of interactors","value":"1287"}]},{"database":"STRING","id":"10090.ENSMUSP00000034361","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"Q922R1","properties":[{"key":"Description","value":"-"}]},{"database":"PhosphoSitePlus","id":"Q922R1","properties":[{"key":"Description","value":"-"}]},{"database":"SwissPalm","id":"Q922R1","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"Q922R1","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"10090-ENSMUSP00000034361","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"Q922R1","properties":[{"key":"Description","value":"-"}]},{"database":"Pumba","id":"Q922R1","properties":[{"key":"Description","value":"-"}]},{"database":"Antibodypedia","id":"29405","properties":[{"key":"antibodies","value":"48 antibodies from 19 providers"}]},{"database":"DNASU","id":"234678","properties":[{"key":"Description","value":"-"}]},{"database":"Ensembl","id":"ENSMUST00000034361.10","properties":[{"key":"ProteinId","value":"ENSMUSP00000034361.4"},{"key":"GeneId","value":"ENSMUSG00000031889.10"}]},{"database":"GeneID","id":"234678","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"mmu:234678","properties":[{"key":"Description","value":"-"}]},{"database":"UCSC","id":"uc009nbt.2","properties":[{"key":"OrganismName","value":"mouse"}]},{"database":"AGR","id":"MGI:2443049","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"80262","properties":[{"key":"Description","value":"-"}]},{"database":"MGI","id":"MGI:2443049","properties":[{"key":"GeneName","value":"Phaf1"}]},{"database":"VEuPathDB","id":"HostDB:ENSMUSG00000031889","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG2819","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"GeneTree","id":"ENSGT00940000153528","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_032056_0_1_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q922R1","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"YRKNDQK","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"411211at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q922R1","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID-ORCS","id":"234678","properties":[{"key":"hits","value":"4 hits in 76 CRISPR screens"}]},{"database":"PRO","id":"PR:Q922R1","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000589","properties":[{"key":"Component","value":"Chromosome 8"}]},{"database":"RNAct","id":"Q922R1","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"Bgee","id":"ENSMUSG00000031889","properties":[{"key":"ExpressionPatterns","value":"Expressed in cleaving embryo and 253 other cell types or tissues"}]},{"database":"ExpressionAtlas","id":"Q922R1","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0030425","properties":[{"key":"GoTerm","value":"C:dendrite"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0000407","properties":[{"key":"GoTerm","value":"C:phagophore assembly site"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0030672","properties":[{"key":"GoTerm","value":"C:synaptic vesicle membrane"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0005802","properties":[{"key":"GoTerm","value":"C:trans-Golgi network"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0035254","properties":[{"key":"GoTerm","value":"F:glutamate receptor binding"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0030165","properties":[{"key":"GoTerm","value":"F:PDZ domain binding"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0043001","properties":[{"key":"GoTerm","value":"P:Golgi to plasma membrane protein transport"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"InterPro","id":"IPR005373","properties":[{"key":"EntryName","value":"PHAF1"}]},{"database":"InterPro","id":"IPR039156","properties":[{"key":"EntryName","value":"PHAF1/BROMI"}]},{"database":"PANTHER","id":"PTHR13465:SF2","properties":[{"key":"EntryName","value":"PHAGOSOME ASSEMBLY FACTOR 1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR13465","properties":[{"key":"EntryName","value":"UPF0183 PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03676","properties":[{"key":"EntryName","value":"PHAF1"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MLDLEVVPERSLGNEQWEFTLGMPLAQAVAILQKHCRIIRNVQVLYSEQSPLSHDLILNLTQDGITLLFDAFNQRLKVIEVCELTKVKLKYCGVHFNSQAIAPTIEQIDQSFGATHPGVYNSTEQLFHLNFRGLSFSFQLDSWTEAPKYEPNFAHGLASLQIPHGATVKRMYIYSGNSLQDTKAPVMPLSCFLGNVYAESVDVLRDGTGPSGLRLRLLAAGCGPGVLADAKMRVFERAVYFGDSCQDVLSMLGSPHKVFYKSEDKMKIHSPSPHKQVPSKCNDYFFNYFTLGVDILFDANTHKVKKFVLHTNYPGHYNFNIYHRCEFKIPLAIKKENAGGQTEICTTYSKWDSIQELLGHPVEKPVVLHRSSSPNNTNPFGSTFCFGLQRMIFEVMQNNHIASVTLYGPPRPGAHLRTAELP","length":422,"molWeight":47416,"crc64":"5639E2BB96D8DEA4","md5":"ECABFBB1FDA6CA666EA3A4E65C9F23E4"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Sequence conflict":1},"uniParcId":"UPI00000238F9"}}