{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q91ZP6","secondaryAccessions":["Q5DTZ9","Q6IR02","Q8BVC5"],"uniProtkbId":"NFIP2_MOUSE","entryAudit":{"firstPublicDate":"2005-08-30","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2005-08-30","entryVersion":137,"sequenceVersion":2},"annotationScore":5.0,"organism":{"scientificName":"Mus musculus","commonName":"Mouse","taxonId":10090,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Glires","Rodentia","Myomorpha","Muroidea","Muridae","Murinae","Mus","Mus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"NEDD4 family-interacting protein 2"}},"alternativeNames":[{"fullName":{"value":"NEDD4 WW domain-binding protein 5A"}}],"flag":"Fragment"},"genes":[{"geneName":{"value":"Ndfip2"},"synonyms":[{"value":"Kiaa1165"},{"value":"N4wbp5A"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9NV92"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12050153"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15252135"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27088444"}],"value":"Activates HECT domain-containing E3 ubiquitin-protein ligases, including ITCH, NEDD4, NEDD4L, SMURF2, WWP1 and WWP2, and consequently modulates the stability of their targets. As a result, may control many cellular processes. Recruits ITCH, NEDD4 and SMURF2 to endosomal membranes. Negatively regulates KCNH2 potassium channel activity by decreasing its cell-surface expression and interfering with channel maturation through recruitment of NEDD4L to the Golgi apparatus and multivesicular body where it mediates KCNH2 degradation (By similarity). May modulate EGFR signaling (By similarity). Together with NDFIP1, limits the cytokine signaling and expansion of effector Th2 T-cells by promoting degradation of JAK1, probably by ITCH- and NEDD4L-mediated ubiquitination (PubMed:27088444)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9NV92"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12050153"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15252135"}],"value":"Forms heterodimers with NDFIP1 (By similarity). Interacts with HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4 (PubMed:12050153, PubMed:15252135). Interacts with NEDD4L (PubMed:12050153). When phosphorylated at Tyr-142, interacts with SRC and LYN SH2 domain (By similarity). May thus act as a scaffold that recruits SRC to NDFIP1, enhancing NDFIP1 phosphorylation (By similarity). Interacts with SLC11A2/DMT1 (By similarity). May interact with phosphorylated EGFR (By similarity). Interacts with KCNH2 (By similarity)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12050153"}],"value":"Endosome membrane","id":"SL-0100"},"topology":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12050153"}],"value":"Multi-pass membrane protein","id":"SL-9909"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15252135"}],"value":"Golgi apparatus membrane","id":"SL-0134"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15252135"}],"value":"Endosome, multivesicular body membrane","id":"SL-0175"},"topology":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15252135"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"commentType":"ALTERNATIVE PRODUCTS","events":["Alternative splicing"],"isoforms":[{"name":{"value":"1"},"isoformIds":["Q91ZP6-1"],"isoformSequenceStatus":"Displayed"},{"name":{"value":"2"},"isoformIds":["Q91ZP6-2"],"sequenceIds":["VSP_015438","VSP_015439"],"isoformSequenceStatus":"Described"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12050153"}],"value":"Ubiquitously expressed, with highest levels in brain, liver, kidney and testis"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"The PPxY motifs are required for E3 ubiquitin-protein ligase activation and for ubiquitination"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"},{"evidenceCode":"ECO:0000305"}],"value":"Ubiquitinated by NEDD4 and NEDD4L; which does not affect turnover (Probable). Also ubiquitinated by ITCH (By similarity)"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9NV92"}],"value":"Undergoes transient tyrosine-phosphorylation following EGF stimulation, most probably catalyzed by SRC. Phosphorylation on Tyr-126, Tyr-146 and Tyr-152 are dependent on the phosphorylation on Tyr-142. Also phosphorylated by LYN and FYN (By similarity)"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27088444"}],"value":"Mutant mice show no signs of inflammation and have normal T-cell populations in thymus, lymph nodes and spleen"}],"commentType":"DISRUPTION PHENOTYPE"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"AAH71237.1","evidences":[{"evidenceCode":"ECO:0000305"}]},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"BAC27240.1","evidences":[{"evidenceCode":"ECO:0000305"}]},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"BAC33869.1","evidences":[{"evidenceCode":"ECO:0000305"}]},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"BAC37471.1","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"OUTSIDE"},"end":{"value":311,"modifier":"EXACT"}},"description":"NEDD4 family-interacting protein 2","featureId":"PRO_0000096795"},{"type":"Topological domain","location":{"start":{"value":1,"modifier":"OUTSIDE"},"end":{"value":206,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":207,"modifier":"EXACT"},"end":{"value":227,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":228,"modifier":"EXACT"},"end":{"value":232,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":233,"modifier":"EXACT"},"end":{"value":253,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":254,"modifier":"EXACT"},"end":{"value":262,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":263,"modifier":"EXACT"},"end":{"value":283,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":284,"modifier":"EXACT"},"end":{"value":311,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":133,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":123,"modifier":"EXACT"},"end":{"value":126,"modifier":"EXACT"}},"description":"Interaction with NEDD4","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Motif","location":{"start":{"value":123,"modifier":"EXACT"},"end":{"value":126,"modifier":"EXACT"}},"description":"PPxY motif 1"},{"type":"Motif","location":{"start":{"value":149,"modifier":"EXACT"},"end":{"value":152,"modifier":"EXACT"}},"description":"PPxY motif 2"},{"type":"Motif","location":{"start":{"value":159,"modifier":"EXACT"},"end":{"value":161,"modifier":"EXACT"}},"description":"PPxY motif 3"},{"type":"Compositional bias","location":{"start":{"value":7,"modifier":"EXACT"},"end":{"value":22,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":26,"modifier":"EXACT"},"end":{"value":37,"modifier":"EXACT"}},"description":"Gly residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":38,"modifier":"EXACT"},"end":{"value":47,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":48,"modifier":"EXACT"},"end":{"value":75,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":92,"modifier":"EXACT"},"end":{"value":101,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":102,"modifier":"EXACT"},"end":{"value":120,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Modified residue","location":{"start":{"value":126,"modifier":"EXACT"},"end":{"value":126,"modifier":"EXACT"}},"description":"Phosphotyrosine; by SRC","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9NV92"}]},{"type":"Modified residue","location":{"start":{"value":142,"modifier":"EXACT"},"end":{"value":142,"modifier":"EXACT"}},"description":"Phosphotyrosine; by SRC","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9NV92"}]},{"type":"Modified residue","location":{"start":{"value":146,"modifier":"EXACT"},"end":{"value":146,"modifier":"EXACT"}},"description":"Phosphotyrosine; by SRC","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9NV92"}]},{"type":"Modified residue","location":{"start":{"value":152,"modifier":"EXACT"},"end":{"value":152,"modifier":"EXACT"}},"description":"Phosphotyrosine; by SRC","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9NV92"}]},{"type":"Alternative sequence","location":{"start":{"value":256,"modifier":"EXACT"},"end":{"value":263,"modifier":"EXACT"}},"description":"in isoform 2","evidences":[{"evidenceCode":"ECO:0000303","source":"Reference","id":"Ref.4"}],"featureId":"VSP_015438","alternativeSequence":{"originalSequence":"FSDYFTGY","alternativeSequences":["VSILIIFM"]}},{"type":"Alternative sequence","location":{"start":{"value":264,"modifier":"EXACT"},"end":{"value":311,"modifier":"EXACT"}},"description":"in isoform 2","evidences":[{"evidenceCode":"ECO:0000303","source":"Reference","id":"Ref.4"}],"featureId":"VSP_015439","alternativeSequence":{}},{"type":"Sequence conflict","location":{"start":{"value":38,"modifier":"EXACT"},"end":{"value":38,"modifier":"EXACT"}},"description":"in Ref. 2; BAC33869","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["T"]}},{"type":"Sequence conflict","location":{"start":{"value":70,"modifier":"EXACT"},"end":{"value":70,"modifier":"EXACT"}},"description":"in Ref. 2; BAC27240","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["S"]}},{"type":"Sequence conflict","location":{"start":{"value":113,"modifier":"EXACT"},"end":{"value":113,"modifier":"EXACT"}},"description":"in Ref. 1; AAH71237","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"A","alternativeSequences":["T"]}},{"type":"Sequence conflict","location":{"start":{"value":298,"modifier":"EXACT"},"end":{"value":298,"modifier":"EXACT"}},"description":"in Ref. 2; BAC37471","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["G"]}},{"type":"Non-terminal residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":""}],"keywords":[{"id":"KW-0025","category":"Coding sequence diversity","name":"Alternative splicing"},{"id":"KW-0967","category":"Cellular component","name":"Endosome"},{"id":"KW-0333","category":"Cellular component","name":"Golgi apparatus"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"id":"KW-0832","category":"PTM","name":"Ubl conjugation"}],"references":[{"referenceNumber":1,"citation":{"id":"15489334","citationType":"journal article","authoringGroup":["The MGC Project Team"],"citationCrossReferences":[{"database":"PubMed","id":"15489334"},{"database":"DOI","id":"10.1101/gr.2596504"}],"title":"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).","publicationDate":"2004","journal":"Genome Res.","firstPage":"2121","lastPage":"2127","volume":"14"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)"],"referenceComments":[{"value":"FVB/N-3","type":"STRAIN"},{"value":"Mammary gland","type":"TISSUE"}]},{"referenceNumber":2,"citation":{"id":"16141072","citationType":"journal article","authors":["Carninci P.","Kasukawa T.","Katayama S.","Gough J.","Frith M.C.","Maeda N.","Oyama R.","Ravasi T.","Lenhard B.","Wells C.","Kodzius R.","Shimokawa K.","Bajic V.B.","Brenner S.E.","Batalov S.","Forrest A.R.","Zavolan M.","Davis M.J.","Wilming L.G.","Aidinis V.","Allen J.E.","Ambesi-Impiombato A.","Apweiler R.","Aturaliya R.N.","Bailey T.L.","Bansal M.","Baxter L.","Beisel K.W.","Bersano T.","Bono H.","Chalk A.M.","Chiu K.P.","Choudhary V.","Christoffels A.","Clutterbuck D.R.","Crowe M.L.","Dalla E.","Dalrymple B.P.","de Bono B.","Della Gatta G.","di Bernardo D.","Down T.","Engstrom P.","Fagiolini M.","Faulkner G.","Fletcher C.F.","Fukushima T.","Furuno M.","Futaki S.","Gariboldi M.","Georgii-Hemming P.","Gingeras T.R.","Gojobori T.","Green R.E.","Gustincich S.","Harbers M.","Hayashi Y.","Hensch T.K.","Hirokawa N.","Hill D.","Huminiecki L.","Iacono M.","Ikeo K.","Iwama A.","Ishikawa T.","Jakt M.","Kanapin A.","Katoh M.","Kawasawa Y.","Kelso J.","Kitamura H.","Kitano H.","Kollias G.","Krishnan S.P.","Kruger A.","Kummerfeld S.K.","Kurochkin I.V.","Lareau L.F.","Lazarevic D.","Lipovich L.","Liu J.","Liuni S.","McWilliam S.","Madan Babu M.","Madera M.","Marchionni L.","Matsuda H.","Matsuzawa S.","Miki H.","Mignone F.","Miyake S.","Morris K.","Mottagui-Tabar S.","Mulder N.","Nakano N.","Nakauchi H.","Ng P.","Nilsson R.","Nishiguchi S.","Nishikawa S.","Nori F.","Ohara O.","Okazaki Y.","Orlando V.","Pang K.C.","Pavan W.J.","Pavesi G.","Pesole G.","Petrovsky N.","Piazza S.","Reed J.","Reid J.F.","Ring B.Z.","Ringwald M.","Rost B.","Ruan Y.","Salzberg S.L.","Sandelin A.","Schneider C.","Schoenbach C.","Sekiguchi K.","Semple C.A.","Seno S.","Sessa L.","Sheng Y.","Shibata Y.","Shimada H.","Shimada K.","Silva D.","Sinclair B.","Sperling S.","Stupka E.","Sugiura K.","Sultana R.","Takenaka Y.","Taki K.","Tammoja K.","Tan S.L.","Tang S.","Taylor M.S.","Tegner J.","Teichmann S.A.","Ueda H.R.","van Nimwegen E.","Verardo R.","Wei C.L.","Yagi K.","Yamanishi H.","Zabarovsky E.","Zhu S.","Zimmer A.","Hide W.","Bult C.","Grimmond S.M.","Teasdale R.D.","Liu E.T.","Brusic V.","Quackenbush J.","Wahlestedt C.","Mattick J.S.","Hume D.A.","Kai C.","Sasaki D.","Tomaru Y.","Fukuda S.","Kanamori-Katayama M.","Suzuki M.","Aoki J.","Arakawa T.","Iida J.","Imamura K.","Itoh M.","Kato T.","Kawaji H.","Kawagashira N.","Kawashima T.","Kojima M.","Kondo S.","Konno H.","Nakano K.","Ninomiya N.","Nishio T.","Okada M.","Plessy C.","Shibata K.","Shiraki T.","Suzuki S.","Tagami M.","Waki K.","Watahiki A.","Okamura-Oho Y.","Suzuki H.","Kawai J.","Hayashizaki Y."],"citationCrossReferences":[{"database":"PubMed","id":"16141072"},{"database":"DOI","id":"10.1126/science.1112014"}],"title":"The transcriptional landscape of the mammalian genome.","publicationDate":"2005","journal":"Science","firstPage":"1559","lastPage":"1563","volume":"309"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 5-311 (ISOFORM 1)"],"referenceComments":[{"value":"C57BL/6J","type":"STRAIN"},{"value":"Cecum","type":"TISSUE"},{"value":"Forelimb","type":"TISSUE"},{"value":"Spinal cord","type":"TISSUE"}]},{"referenceNumber":3,"citation":{"id":"12050153","citationType":"journal article","authors":["Konstas A.-A.","Shearwin-Whyatt L.M.","Fotia A.B.","Degger B.","Riccardi D.","Cook D.I.","Korbmacher C.","Kumar S."],"citationCrossReferences":[{"database":"PubMed","id":"12050153"},{"database":"DOI","id":"10.1074/jbc.m203018200"}],"title":"Regulation of the epithelial sodium channel by N4WBP5A, a novel Nedd4/Nedd4-2-interacting protein.","publicationDate":"2002","journal":"J. Biol. Chem.","firstPage":"29406","lastPage":"29416","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA] OF 71-311 (ISOFORM 1)","FUNCTION","INTERACTION WITH NEDD4 AND NEDD4L","TISSUE SPECIFICITY","SUBCELLULAR LOCATION"],"referenceComments":[{"value":"CBA/J","type":"STRAIN"},{"value":"Brain","type":"TISSUE"}]},{"referenceNumber":4,"citation":{"id":"CI-C3VEIMGK1G3LQ","citationType":"submission","authors":["Okazaki N.","Kikuno R.F.","Ohara R.","Inamoto S.","Nagase T.","Ohara O.","Koga H."],"title":"Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.","publicationDate":"FEB-2005","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 158-311 (ISOFORM 2)"],"referenceComments":[{"value":"Embryonic intestine","type":"TISSUE"}]},{"referenceNumber":5,"citation":{"id":"15252135","citationType":"journal article","authors":["Shearwin-Whyatt L.M.","Brown D.L.","Wylie F.G.","Stow J.L.","Kumar S."],"citationCrossReferences":[{"database":"PubMed","id":"15252135"},{"database":"DOI","id":"10.1242/jcs.01212"}],"title":"N4WBP5A (Ndfip2), a Nedd4-interacting protein, localizes to multivesicular bodies and the Golgi, and has a potential role in protein trafficking.","publicationDate":"2004","journal":"J. Cell Sci.","firstPage":"3679","lastPage":"3689","volume":"117"},"referencePositions":["FUNCTION","INTERACTION WITH NEDD4","SUBCELLULAR LOCATION","UBIQUITINATION"]},{"referenceNumber":6,"citation":{"id":"27088444","citationType":"journal article","authors":["O'Leary C.E.","Riling C.R.","Spruce L.A.","Ding H.","Kumar S.","Deng G.","Liu Y.","Seeholzer S.H.","Oliver P.M."],"citationCrossReferences":[{"database":"PubMed","id":"27088444"},{"database":"DOI","id":"10.1038/ncomms11226"}],"title":"Ndfip-mediated degradation of Jak1 tunes cytokine signalling to limit expansion of CD4+ effector T cells.","publicationDate":"2016","journal":"Nat. Commun.","firstPage":"11226","lastPage":"11226","volume":"7"},"referencePositions":["FUNCTION","DISRUPTION PHENOTYPE"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"BC071237","properties":[{"key":"ProteinId","value":"AAH71237.1"},{"key":"Status","value":"ALT_INIT"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK031073","properties":[{"key":"ProteinId","value":"BAC27240.1"},{"key":"Status","value":"ALT_INIT"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK049678","properties":[{"key":"ProteinId","value":"BAC33869.1"},{"key":"Status","value":"ALT_INIT"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK078939","properties":[{"key":"ProteinId","value":"BAC37471.1"},{"key":"Status","value":"ALT_INIT"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AF411608","properties":[{"key":"ProteinId","value":"AAL05872.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK220371","properties":[{"key":"ProteinId","value":"BAD90430.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"AlphaFoldDB","id":"Q91ZP6","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q91ZP6","properties":[{"key":"Description","value":"-"}]},{"database":"DIP","id":"DIP-58955N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q91ZP6","properties":[{"key":"Number of interactors","value":"1676"}]},{"database":"IntAct","id":"Q91ZP6","properties":[{"key":"Interactions","value":"1"}]},{"database":"STRING","id":"10090.ENSMUSP00000137875","properties":[{"key":"Description","value":"-"}]},{"database":"TCDB","id":"8.A.30.1.1","properties":[{"key":"FamilyName","value":"the nedd4-family interacting protein-2 (nedd4) family"}]},{"database":"iPTMnet","id":"Q91ZP6","properties":[{"key":"Description","value":"-"}]},{"database":"PhosphoSitePlus","id":"Q91ZP6","properties":[{"key":"Description","value":"-"}]},{"database":"SwissPalm","id":"Q91ZP6","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"10090-ENSMUSP00000137875","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"252816","properties":[{"key":"Description","value":"-"}],"isoformId":"Q91ZP6-1"},{"database":"ProteomicsDB","id":"252817","properties":[{"key":"Description","value":"-"}],"isoformId":"Q91ZP6-2"},{"database":"Pumba","id":"Q91ZP6","properties":[{"key":"Description","value":"-"}]},{"database":"AGR","id":"MGI:1923523","properties":[{"key":"Description","value":"-"}]},{"database":"MGI","id":"MGI:1923523","properties":[{"key":"GeneName","value":"Ndfip2"}]},{"database":"eggNOG","id":"KOG4812","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"InParanoid","id":"Q91ZP6","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"FASHENS","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"PhylomeDB","id":"Q91ZP6","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:Q91ZP6","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000589","properties":[{"key":"Component","value":"Unplaced"}]},{"database":"RNAct","id":"Q91ZP6","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0005783","properties":[{"key":"GoTerm","value":"C:endoplasmic reticulum"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0010008","properties":[{"key":"GoTerm","value":"C:endosome membrane"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0005794","properties":[{"key":"GoTerm","value":"C:Golgi apparatus"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0000139","properties":[{"key":"GoTerm","value":"C:Golgi membrane"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0016020","properties":[{"key":"GoTerm","value":"C:membrane"},{"key":"GoEvidenceType","value":"ISM:MGI"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PubMed","id":"11748237"}]},{"database":"GO","id":"GO:0005739","properties":[{"key":"GoTerm","value":"C:mitochondrion"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0032585","properties":[{"key":"GoTerm","value":"C:multivesicular body membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0048471","properties":[{"key":"GoTerm","value":"C:perinuclear region of cytoplasm"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0050699","properties":[{"key":"GoTerm","value":"F:WW domain binding"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0030001","properties":[{"key":"GoTerm","value":"P:metal ion transport"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0010629","properties":[{"key":"GoTerm","value":"P:negative regulation of gene expression"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0051224","properties":[{"key":"GoTerm","value":"P:negative regulation of protein transport"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0031398","properties":[{"key":"GoTerm","value":"P:positive regulation of protein ubiquitination"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0043161","properties":[{"key":"GoTerm","value":"P:proteasome-mediated ubiquitin-dependent protein catabolic process"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0006511","properties":[{"key":"GoTerm","value":"P:ubiquitin-dependent protein catabolic process"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0007034","properties":[{"key":"GoTerm","value":"P:vacuolar transport"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd22306","properties":[{"key":"EntryName","value":"NDFIP2"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR019325","properties":[{"key":"EntryName","value":"NEDD4/Bsd2"}]},{"database":"PANTHER","id":"PTHR13396","properties":[{"key":"EntryName","value":"NEDD4 FAMILY INTERACTING PROTEIN 1/2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR13396:SF4","properties":[{"key":"EntryName","value":"NEDD4 FAMILY-INTERACTING PROTEIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF10176","properties":[{"key":"EntryName","value":"NEDD4_Bsd2"},{"key":"MatchStatus","value":"2"}]}],"sequence":{"value":"RRSASDAELSAGAEGATGSEAAPPGDLGGRTRGGGRGSAAAAATTSTREAEGAERRGDTPARKPDPEAGRMDHHQLGTGRYQVLHNEEDNSESSAVEQPSTSSLAAPTVEAAASAPALDPDSPPPYSSITVEAPTTSDTDVYSEFYPVPPPYSVATSLPTYDEAEKAKAAALAAAAADAPQRNQEEDCTPRDDFSDVEQLRVGNDGIFMLAFFMAFIFNWLGFCLSFCITNTIAGRYGAICGFGLSLIKWILIVRFSDYFTGYFNGQYWLWWIFLVLGLLLFFRGFVNYLKVRNMSESMAAAHRTRYFFLL","length":311,"molWeight":33638,"crc64":"AC9A445A83F1B789","md5":"EAC1F876FE1F36668D077C356EA4461C"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"ALTERNATIVE PRODUCTS":2,"TISSUE SPECIFICITY":1,"DOMAIN":1,"PTM":2,"DISRUPTION PHENOTYPE":1,"SEQUENCE CAUTION":4},"countByFeatureType":{"Chain":1,"Topological domain":4,"Transmembrane":3,"Region":2,"Motif":3,"Compositional bias":6,"Modified residue":4,"Alternative sequence":2,"Sequence conflict":4,"Non-terminal residue":1},"uniParcId":"UPI000058E3E0"}}