{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q91YJ3","uniProtkbId":"THYN1_MOUSE","entryAudit":{"firstPublicDate":"2006-11-28","lastAnnotationUpdateDate":"2026-06-10","lastSequenceUpdateDate":"2001-12-01","entryVersion":139,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Mus musculus","commonName":"Mouse","taxonId":10090,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Glires","Rodentia","Myomorpha","Muroidea","Muridae","Murinae","Mus","Mus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Thymocyte nuclear protein 1"}},"alternativeNames":[{"fullName":{"value":"Thymocyte protein Thy28"},"shortNames":[{"value":"mThy28"}]}]},"genes":[{"geneName":{"value":"Thyn1"},"synonyms":[{"value":"Thy28"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23434322"}],"value":"Specifically binds 5-hydroxymethylcytosine (5hmC), suggesting that it acts as a specific reader of 5hmC"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14580360"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14601557"}],"value":"Nucleus","id":"SL-0191"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12384300"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14580360"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14601557"}],"value":"Expressed in the medulla containing mature thymocytes, but not the cortex having immature thymocytes (at protein level). Abundant expression seen in testis, liver, brain and kidney with lower levels of the expression in thymus, spleen, heart and stomach"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14580360"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14601557"}],"value":"Down-regulated upon induction of apoptosis"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Phosphorylated"}],"commentType":"PTM"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":226,"modifier":"EXACT"}},"description":"Thymocyte nuclear protein 1","featureId":"PRO_0000262565"},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":38,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Motif","location":{"start":{"value":5,"modifier":"EXACT"},"end":{"value":10,"modifier":"EXACT"}},"description":"Nuclear localization signal","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Compositional bias","location":{"start":{"value":14,"modifier":"EXACT"},"end":{"value":28,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":29,"modifier":"EXACT"},"end":{"value":38,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]}],"keywords":[{"id":"KW-0539","category":"Cellular component","name":"Nucleus"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"12384300","citationType":"journal article","authors":["Miyaji H.","Yoshimoto T.","Asakura H.","Komachi A.","Kamiya S.","Takasaki M.","Mizuguchi J."],"citationCrossReferences":[{"database":"PubMed","id":"12384300"},{"database":"DOI","id":"10.1016/s0378-1119(02)00886-7"}],"title":"Molecular cloning and characterization of the mouse thymocyte protein gene.","publicationDate":"2002","journal":"Gene","firstPage":"189","lastPage":"196","volume":"297"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]","TISSUE SPECIFICITY"]},{"referenceNumber":2,"citation":{"id":"15489334","citationType":"journal article","authoringGroup":["The MGC Project Team"],"citationCrossReferences":[{"database":"PubMed","id":"15489334"},{"database":"DOI","id":"10.1101/gr.2596504"}],"title":"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).","publicationDate":"2004","journal":"Genome Res.","firstPage":"2121","lastPage":"2127","volume":"14"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"FVB/N","type":"STRAIN"},{"value":"Mammary tumor","type":"TISSUE"}]},{"referenceNumber":3,"citation":{"id":"14601557","citationType":"journal article","authors":["Jiang X.Z.","Toyota H.","Yoshimoto T.","Takada E.","Asakura H.","Mizuguchi J."],"citationCrossReferences":[{"database":"PubMed","id":"14601557"},{"database":"DOI","id":"10.1023/a:1025594409056"}],"title":"Anti-IgM-induced down-regulation of nuclear Thy28 protein expression in Ramos B lymphoma cells.","publicationDate":"2003","journal":"Apoptosis","firstPage":"509","lastPage":"519","volume":"8"},"referencePositions":["INDUCTION","SUBCELLULAR LOCATION","TISSUE SPECIFICITY"]},{"referenceNumber":4,"citation":{"id":"14580360","citationType":"journal article","authors":["Jiang X.Z.","Toyota H.","Takada E.","Yoshimoto T.","Kitamura T.","Yamada J.","Mizuguchi J."],"citationCrossReferences":[{"database":"PubMed","id":"14580360"},{"database":"DOI","id":"10.1016/s0040-8166(03)00073-9"}],"title":"Modulation of mThy28 nuclear protein expression during thymocyte development.","publicationDate":"2003","journal":"Tissue Cell","firstPage":"471","lastPage":"478","volume":"35"},"referencePositions":["INDUCTION","SUBCELLULAR LOCATION","TISSUE SPECIFICITY"]},{"referenceNumber":5,"citation":{"id":"21183079","citationType":"journal article","authors":["Huttlin E.L.","Jedrychowski M.P.","Elias J.E.","Goswami T.","Rad R.","Beausoleil S.A.","Villen J.","Haas W.","Sowa M.E.","Gygi S.P."],"citationCrossReferences":[{"database":"PubMed","id":"21183079"},{"database":"DOI","id":"10.1016/j.cell.2010.12.001"}],"title":"A tissue-specific atlas of mouse protein phosphorylation and expression.","publicationDate":"2010","journal":"Cell","firstPage":"1174","lastPage":"1189","volume":"143"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"Testis","type":"TISSUE"}]},{"referenceNumber":6,"citation":{"id":"23434322","citationType":"journal article","authors":["Spruijt C.G.","Gnerlich F.","Smits A.H.","Pfaffeneder T.","Jansen P.W.","Bauer C.","Munzel M.","Wagner M.","Muller M.","Khan F.","Eberl H.C.","Mensinga A.","Brinkman A.B.","Lephikov K.","Muller U.","Walter J.","Boelens R.","van Ingen H.","Leonhardt H.","Carell T.","Vermeulen M."],"citationCrossReferences":[{"database":"PubMed","id":"23434322"},{"database":"DOI","id":"10.1016/j.cell.2013.02.004"}],"title":"Dynamic readers for 5-(hydroxy)methylcytosine and its oxidized derivatives.","publicationDate":"2013","journal":"Cell","firstPage":"1146","lastPage":"1159","volume":"152"},"referencePositions":["FUNCTION"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB080370","properties":[{"key":"ProteinId","value":"BAC21100.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC016594","properties":[{"key":"ProteinId","value":"AAH16594.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"CCDS","id":"CCDS22937.1","properties":[{"key":"Description","value":"-"}]},{"database":"RefSeq","id":"NP_653126.1","properties":[{"key":"NucleotideSequenceId","value":"NM_144543.2"}]},{"database":"AlphaFoldDB","id":"Q91YJ3","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q91YJ3","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"218981","properties":[{"key":"Interactions","value":"2"}]},{"database":"FunCoup","id":"Q91YJ3","properties":[{"key":"Number of interactors","value":"2231"}]},{"database":"STRING","id":"10090.ENSMUSP00000150823","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"Q91YJ3","properties":[{"key":"Description","value":"-"}]},{"database":"PhosphoSitePlus","id":"Q91YJ3","properties":[{"key":"Description","value":"-"}]},{"database":"SwissPalm","id":"Q91YJ3","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"10090-ENSMUSP00000150823","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"Q91YJ3","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"259187","properties":[{"key":"Description","value":"-"}]},{"database":"Pumba","id":"Q91YJ3","properties":[{"key":"Description","value":"-"}]},{"database":"Antibodypedia","id":"33170","properties":[{"key":"antibodies","value":"157 antibodies from 25 providers"}]},{"database":"DNASU","id":"77862","properties":[{"key":"Description","value":"-"}]},{"database":"Ensembl","id":"ENSMUST00000039161.10","properties":[{"key":"ProteinId","value":"ENSMUSP00000037614.9"},{"key":"GeneId","value":"ENSMUSG00000035443.11"}]},{"database":"Ensembl","id":"ENSMUST00000213683.2","properties":[{"key":"ProteinId","value":"ENSMUSP00000150823.2"},{"key":"GeneId","value":"ENSMUSG00000035443.11"}]},{"database":"GeneID","id":"77862","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"mmu:77862","properties":[{"key":"Description","value":"-"}]},{"database":"UCSC","id":"uc009oqb.2","properties":[{"key":"OrganismName","value":"mouse"}]},{"database":"AGR","id":"MGI:1925112","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"29087","properties":[{"key":"Description","value":"-"}]},{"database":"MGI","id":"MGI:1925112","properties":[{"key":"GeneName","value":"Thyn1"}]},{"database":"VEuPathDB","id":"HostDB:ENSMUSG00000035443","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG3383","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"GeneTree","id":"ENSGT00390000013297","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_041799_2_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q91YJ3","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"DVQFIRM","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"41445at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q91YJ3","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID-ORCS","id":"77862","properties":[{"key":"hits","value":"1 hit in 77 CRISPR screens"}]},{"database":"PRO","id":"PR:Q91YJ3","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000589","properties":[{"key":"Component","value":"Chromosome 9"}]},{"database":"RNAct","id":"Q91YJ3","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"Bgee","id":"ENSMUSG00000035443","properties":[{"key":"ExpressionPatterns","value":"Expressed in floor plate of midbrain and 254 other cell types or tissues"}]},{"database":"ExpressionAtlas","id":"Q91YJ3","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0001650","properties":[{"key":"GoTerm","value":"C:fibrillar center"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0005654","properties":[{"key":"GoTerm","value":"C:nucleoplasm"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0005634","properties":[{"key":"GoTerm","value":"C:nucleus"},{"key":"GoEvidenceType","value":"IDA:HGNC-UCL"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"14601557"}]},{"database":"CDD","id":"cd21133","properties":[{"key":"EntryName","value":"EVE"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.10.590.10:FF:000003","properties":[{"key":"EntryName","value":"Thymocyte nuclear protein 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.10.590.10","properties":[{"key":"EntryName","value":"ph1033 like domains"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR052181","properties":[{"key":"EntryName","value":"5hmC_binding"}]},{"database":"InterPro","id":"IPR002740","properties":[{"key":"EntryName","value":"EVE_domain"}]},{"database":"InterPro","id":"IPR015947","properties":[{"key":"EntryName","value":"PUA-like_sf"}]},{"database":"InterPro","id":"IPR047197","properties":[{"key":"EntryName","value":"THYN1-like_EVE"}]},{"database":"PANTHER","id":"PTHR14087","properties":[{"key":"EntryName","value":"THYMOCYTE NUCLEAR PROTEIN 1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR14087:SF7","properties":[{"key":"EntryName","value":"THYMOCYTE NUCLEAR PROTEIN 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01878","properties":[{"key":"EntryName","value":"EVE"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF88697","properties":[{"key":"EntryName","value":"PUA domain-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MPRPRKRQTGTAGPDRKKLSGKRTKTENSESTSVKLENSSLEMTTTFKNGGKNLSNYWLMKSEPESRLEKGIDMKFSIEDLKAQPKQTACWDGVRNYQARNFLRAMKLEDEAFFYHSNCKQPGIVGLMKIVKEAYPDHTQFEKSNPHYDPSSKEDDPKWSMVDVQFVRMMKRFIPLEELKTYHQAHKATGGPLKSMTLFTRQRLSVQPLTQEEFDFILSLEETEPS","length":226,"molWeight":26178,"crc64":"57D69619D37CDF89","md5":"18CBF62965D5CEE42BC1D507C1AC9198"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"INDUCTION":1,"PTM":1},"countByFeatureType":{"Chain":1,"Region":1,"Motif":1,"Compositional bias":2},"uniParcId":"UPI00000249A2"}}