{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q8ZLY9","uniProtkbId":"UBIK_SALTY","entryAudit":{"firstPublicDate":"2018-11-07","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2002-03-01","entryVersion":93,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)","taxonId":99287,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Salmonella"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_02216"},{"evidenceCode":"ECO:0000305"}],"value":"Ubiquinone biosynthesis accessory factor UbiK"}}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_02216"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"28559279"}],"value":"ubiK"},"synonyms":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"21554724"}],"value":"yqiC"}],"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AAL22070.1"}],"value":"STM3196"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21554724"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27777572"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28559279"}],"value":"Required for efficient ubiquinone (coenzyme Q) biosynthesis under aerobic conditions. UbiK is probably an accessory factor of Ubi enzymes and facilitates ubiquinone biosynthesis by acting as an assembly factor, a targeting factor, or both. Dispensable for ubiquinone biosynthesis under anaerobiosis (PubMed:28559279). Required for proliferation in macrophages and virulence in mice (PubMed:21554724, PubMed:28559279). Significantly contributes to colonization and invasion as well as host inflammation and innate immunity after infection (PubMed:27777572). In vitro, has membrane fusogenic activity at acidic pH (PubMed:21554724)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_02216"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"28559279"}],"value":"Cofactor biosynthesis; ubiquinone biosynthesis"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21554724"}],"value":"Homotrimer"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21554724"}],"value":"Is both soluble and membrane associated inside the cell"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_02216"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21554724"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21554724"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27777572"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28559279"}],"value":"Mutant is growth-deficient under aerobic conditions and is impaired to replicate at physiological and high temperatures (PubMed:21554724, PubMed:28559279). Mutant is defective for proliferation in macrophages and mice infection and has a severe attenuation in virulence in the murine model when inoculated both orally and intraperitoneally (PubMed:21554724, PubMed:28559279). Deletion inhibits colonization and invasion of four human cell lines, impairs flagella formation, activates the expression of type-1 fimbriae-like structures on the cell surface, enhances biofilm formation and reduces bacterial motility. The deletion mutant is menaquinone-deficient (PubMed:27777572)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_02216"},{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the UbiK family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"Ubiquinone biosynthesis accessory factor UbiK","featureId":"PRO_0000445597"},{"type":"Region","location":{"start":{"value":96,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Coiled coil","location":{"start":{"value":79,"modifier":"EXACT"},"end":{"value":99,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_02216"}]},{"type":"Compositional bias","location":{"start":{"value":96,"modifier":"EXACT"},"end":{"value":106,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":109,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"Pro residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]}],"keywords":[{"id":"KW-0175","category":"Domain","name":"Coiled coil"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0831","category":"Biological process","name":"Ubiquinone biosynthesis"}],"references":[{"referenceNumber":1,"citation":{"id":"11677609","citationType":"journal article","authors":["McClelland M.","Sanderson K.E.","Spieth J.","Clifton S.W.","Latreille P.","Courtney L.","Porwollik S.","Ali J.","Dante M.","Du F.","Hou S.","Layman D.","Leonard S.","Nguyen C.","Scott K.","Holmes A.","Grewal N.","Mulvaney E.","Ryan E.","Sun H.","Florea L.","Miller W.","Stoneking T.","Nhan M.","Waterston R.","Wilson R.K."],"citationCrossReferences":[{"database":"PubMed","id":"11677609"},{"database":"DOI","id":"10.1038/35101614"}],"title":"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.","publicationDate":"2001","journal":"Nature","firstPage":"852","lastPage":"856","volume":"413"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"LT2 / SGSC1412 / ATCC 700720","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"21554724","citationType":"journal article","authors":["Carrica M.C.","Craig P.O.","Garcia-Angulo V.A.","Aguirre A.","Garcia-Vescovi E.","Goldbaum F.A.","Cravero S.L."],"citationCrossReferences":[{"database":"PubMed","id":"21554724"},{"database":"DOI","id":"10.1186/1471-2180-11-95"}],"title":"YqiC of Salmonella enterica serovar Typhimurium is a membrane fusogenic protein required for mice colonization.","publicationDate":"2011","journal":"BMC Microbiol.","firstPage":"95","lastPage":"95","volume":"11"},"referencePositions":["FUNCTION","SUBUNIT","SUBCELLULAR LOCATION","DISRUPTION PHENOTYPE"],"referenceComments":[{"value":"ATCC 14028 / SGSC 2980 / CDC 6516-60 / NCTC 12023","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"27777572","citationType":"journal article","authors":["Wang K.C.","Huang C.H.","Ding S.M.","Chen C.K.","Fang H.W.","Huang M.T.","Fang S.B."],"citationCrossReferences":[{"database":"PubMed","id":"27777572"},{"database":"DOI","id":"10.3389/fmicb.2016.01614"}],"title":"Role of yqiC in the pathogenicity of Salmonella and innate immune responses of human intestinal epithelium.","publicationDate":"2016","journal":"Front. Microbiol.","firstPage":"1614","lastPage":"1614","volume":"7"},"referencePositions":["FUNCTION","DISRUPTION PHENOTYPE"],"referenceComments":[{"value":"SL1344","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"28559279","citationType":"journal article","authors":["Loiseau L.","Fyfe C.","Aussel L.","Hajj Chehade M.","Hernandez S.B.","Faivre B.","Hamdane D.","Mellot-Draznieks C.","Rascalou B.","Pelosi L.","Velours C.","Cornu D.","Lombard M.","Casadesus J.","Pierrel F.","Fontecave M.","Barras F."],"citationCrossReferences":[{"database":"PubMed","id":"28559279"},{"database":"DOI","id":"10.1074/jbc.m117.789164"}],"title":"The UbiK protein is an accessory factor necessary for bacterial ubiquinone (UQ) biosynthesis and forms a complex with the UQ biogenesis factor UbiJ.","publicationDate":"2017","journal":"J. Biol. Chem.","firstPage":"11937","lastPage":"11950","volume":"292"},"referencePositions":["FUNCTION","PATHWAY","DISRUPTION PHENOTYPE"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AE006468","properties":[{"key":"ProteinId","value":"AAL22070.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"NP_462111.3","properties":[{"key":"NucleotideSequenceId","value":"NC_003197.2"}]},{"database":"RefSeq","id":"WP_014343900.1","properties":[{"key":"NucleotideSequenceId","value":"NC_003197.2"}]},{"database":"AlphaFoldDB","id":"Q8ZLY9","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q8ZLY9","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"99287.STM3196","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"99287-STM3196","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"1254719","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"stm:STM3196","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_154412_0_2_6","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q8ZLY9","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"SENT99287:STM3196-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00232","properties":[{"key":"RectionId","value":"-"}]},{"database":"Proteomes","id":"UP000001014","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21554724"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0006744","properties":[{"key":"GoTerm","value":"P:ubiquinone biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"HAMAP","id":"MF_02216","properties":[{"key":"EntryName","value":"UbiK"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR007475","properties":[{"key":"EntryName","value":"UbiK"}]},{"database":"NCBIfam","id":"NF047835","properties":[{"key":"EntryName","value":"UbiqAccUbiK"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR38040","properties":[{"key":"EntryName","value":"UBIQUINONE BIOSYNTHESIS ACCESSORY FACTOR UBIK"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR38040:SF1","properties":[{"key":"EntryName","value":"UBIQUINONE BIOSYNTHESIS ACCESSORY FACTOR UBIK"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04380","properties":[{"key":"EntryName","value":"BMFP"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MASTYRTTIRANTYQFRETTMIDPKKIEQIARQVHESMPKGIREFGEDIEKKIRQTLQSQLTRLDLVSREEFDVQTQVLLRTREKLALLEQRLSELEARDKPEEVKPAPAIPPVDPQQE","length":119,"molWeight":13947,"crc64":"8226482F8CF67D9F","md5":"80449E620FF5255F2A5AD48346E1826B"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"PATHWAY":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Region":1,"Coiled coil":1,"Compositional bias":2},"uniParcId":"UPI00000CD3C5"}}