{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q8VYB5","secondaryAccessions":["Q8LFY1","Q9C747"],"uniProtkbId":"AMSH1_ARATH","entryAudit":{"firstPublicDate":"2010-08-10","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2002-03-01","entryVersion":129,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Arabidopsis thaliana","commonName":"Mouse-ear cress","taxonId":3702,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","rosids","malvids","Brassicales","Brassicaceae","Camelineae","Arabidopsis"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"value":"AMSH-like ubiquitin thioesterase 1"},"ecNumbers":[{"value":"3.4.19.-"}]},"alternativeNames":[{"fullName":{"value":"Deubiquitinating enzyme AMSH1"}}]},"genes":[{"geneName":{"value":"AMSH1"},"orderedLocusNames":[{"value":"At1g48790"}],"orfNames":[{"value":"F11I4.4"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Zinc metalloprotease that cleaves 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains"}],"commentType":"FUNCTION"},{"commentType":"COFACTOR","cofactors":[{"name":"Zn(2+)","evidences":[{"evidenceCode":"ECO:0000250"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29105"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Binds 2 Zn(2+) ions per subunit."}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Membrane","id":"SL-0162"},"topology":{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Peripheral membrane protein","id":"SL-9903"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"The JAMM motif is essential for the protease activity"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the peptidase M67C family"}],"commentType":"SIMILARITY"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous gene model prediction","sequence":"AAG60133.1","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":507,"modifier":"EXACT"}},"description":"AMSH-like ubiquitin thioesterase 1","featureId":"PRO_0000397098"},{"type":"Domain","location":{"start":{"value":333,"modifier":"EXACT"},"end":{"value":463,"modifier":"EXACT"}},"description":"MPN","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01182"}]},{"type":"Motif","location":{"start":{"value":411,"modifier":"EXACT"},"end":{"value":424,"modifier":"EXACT"}},"description":"JAMM motif","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01182"}]},{"type":"Binding site","location":{"start":{"value":411,"modifier":"EXACT"},"end":{"value":411,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01182"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"1","note":"catalytic"}},{"type":"Binding site","location":{"start":{"value":413,"modifier":"EXACT"},"end":{"value":413,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01182"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"1","note":"catalytic"}},{"type":"Binding site","location":{"start":{"value":424,"modifier":"EXACT"},"end":{"value":424,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01182"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"1","note":"catalytic"}},{"type":"Binding site","location":{"start":{"value":426,"modifier":"EXACT"},"end":{"value":426,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"2"}},{"type":"Binding site","location":{"start":{"value":469,"modifier":"EXACT"},"end":{"value":469,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"2"}},{"type":"Binding site","location":{"start":{"value":475,"modifier":"EXACT"},"end":{"value":475,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"2"}},{"type":"Binding site","location":{"start":{"value":477,"modifier":"EXACT"},"end":{"value":477,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"2"}},{"type":"Site","location":{"start":{"value":356,"modifier":"EXACT"},"end":{"value":356,"modifier":"EXACT"}},"description":"Indirect zinc-binding","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Sequence conflict","location":{"start":{"value":11,"modifier":"EXACT"},"end":{"value":11,"modifier":"EXACT"}},"description":"in Ref. 5; AAM61146","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"A","alternativeSequences":["G"]}}],"keywords":[{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0482","category":"Molecular function","name":"Metalloprotease"},{"id":"KW-0645","category":"Molecular function","name":"Protease"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0833","category":"Biological process","name":"Ubl conjugation pathway"},{"id":"KW-0862","category":"Ligand","name":"Zinc"}],"references":[{"referenceNumber":1,"citation":{"id":"11130712","citationType":"journal article","authors":["Theologis A.","Ecker J.R.","Palm C.J.","Federspiel N.A.","Kaul S.","White O.","Alonso J.","Altafi H.","Araujo R.","Bowman C.L.","Brooks S.Y.","Buehler E.","Chan A.","Chao Q.","Chen H.","Cheuk R.F.","Chin C.W.","Chung M.K.","Conn L.","Conway A.B.","Conway A.R.","Creasy T.H.","Dewar K.","Dunn P.","Etgu P.","Feldblyum T.V.","Feng J.-D.","Fong B.","Fujii C.Y.","Gill J.E.","Goldsmith A.D.","Haas B.","Hansen N.F.","Hughes B.","Huizar L.","Hunter J.L.","Jenkins J.","Johnson-Hopson C.","Khan S.","Khaykin E.","Kim C.J.","Koo H.L.","Kremenetskaia I.","Kurtz D.B.","Kwan A.","Lam B.","Langin-Hooper S.","Lee A.","Lee J.M.","Lenz C.A.","Li J.H.","Li Y.-P.","Lin X.","Liu S.X.","Liu Z.A.","Luros J.S.","Maiti R.","Marziali A.","Militscher J.","Miranda M.","Nguyen M.","Nierman W.C.","Osborne B.I.","Pai G.","Peterson J.","Pham P.K.","Rizzo M.","Rooney T.","Rowley D.","Sakano H.","Salzberg S.L.","Schwartz J.R.","Shinn P.","Southwick A.M.","Sun H.","Tallon L.J.","Tambunga G.","Toriumi M.J.","Town C.D.","Utterback T.","Van Aken S.","Vaysberg M.","Vysotskaia V.S.","Walker M.","Wu D.","Yu G.","Fraser C.M.","Venter J.C.","Davis R.W."],"citationCrossReferences":[{"database":"PubMed","id":"11130712"},{"database":"DOI","id":"10.1038/35048500"}],"title":"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.","publicationDate":"2000","journal":"Nature","firstPage":"816","lastPage":"820","volume":"408"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"27862469","citationType":"journal article","authors":["Cheng C.Y.","Krishnakumar V.","Chan A.P.","Thibaud-Nissen F.","Schobel S.","Town C.D."],"citationCrossReferences":[{"database":"PubMed","id":"27862469"},{"database":"DOI","id":"10.1111/tpj.13415"}],"title":"Araport11: a complete reannotation of the Arabidopsis thaliana reference genome.","publicationDate":"2017","journal":"Plant J.","firstPage":"789","lastPage":"804","volume":"89"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"CI-CI4AM1G71UC0S","citationType":"submission","authoringGroup":["The Arabidopsis Information Resource (TAIR)"],"title":"Community-consensus reference genome of Arabidopsis thaliana Col-0.","publicationDate":"FEB-2026","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"14593172","citationType":"journal article","authors":["Yamada K.","Lim J.","Dale J.M.","Chen H.","Shinn P.","Palm C.J.","Southwick A.M.","Wu H.C.","Kim C.J.","Nguyen M.","Pham P.K.","Cheuk R.F.","Karlin-Newmann G.","Liu S.X.","Lam B.","Sakano H.","Wu T.","Yu G.","Miranda M.","Quach H.L.","Tripp M.","Chang C.H.","Lee J.M.","Toriumi M.J.","Chan M.M.","Tang C.C.","Onodera C.S.","Deng J.M.","Akiyama K.","Ansari Y.","Arakawa T.","Banh J.","Banno F.","Bowser L.","Brooks S.Y.","Carninci P.","Chao Q.","Choy N.","Enju A.","Goldsmith A.D.","Gurjal M.","Hansen N.F.","Hayashizaki Y.","Johnson-Hopson C.","Hsuan V.W.","Iida K.","Karnes M.","Khan S.","Koesema E.","Ishida J.","Jiang P.X.","Jones T.","Kawai J.","Kamiya A.","Meyers C.","Nakajima M.","Narusaka M.","Seki M.","Sakurai T.","Satou M.","Tamse R.","Vaysberg M.","Wallender E.K.","Wong C.","Yamamura Y.","Yuan S.","Shinozaki K.","Davis R.W.","Theologis A.","Ecker J.R."],"citationCrossReferences":[{"database":"PubMed","id":"14593172"},{"database":"DOI","id":"10.1126/science.1088305"}],"title":"Empirical analysis of transcriptional activity in the Arabidopsis genome.","publicationDate":"2003","journal":"Science","firstPage":"842","lastPage":"846","volume":"302"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"CI-3TH3AFLAVIRO9","citationType":"submission","authors":["Brover V.V.","Troukhan M.E.","Alexandrov N.A.","Lu Y.-P.","Flavell R.B.","Feldmann K.A."],"title":"Full-length cDNA from Arabidopsis thaliana.","publicationDate":"MAR-2002","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"]},{"referenceNumber":6,"citation":{"id":"20543027","citationType":"journal article","authors":["Isono E.","Katsiarimpa A.","Mueller I.K.","Anzenberger F.","Stierhof Y.-D.","Geldner N.","Chory J.","Schwechheimer C."],"citationCrossReferences":[{"database":"PubMed","id":"20543027"},{"database":"DOI","id":"10.1105/tpc.110.075952"}],"title":"The deubiquitinating enzyme AMSH3 is required for intracellular trafficking and vacuole biogenesis in Arabidopsis thaliana.","publicationDate":"2010","journal":"Plant Cell","firstPage":"1826","lastPage":"1837","volume":"22"},"referencePositions":["GENE FAMILY","NOMENCLATURE"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AC073555","properties":[{"key":"ProteinId","value":"AAG60133.1"},{"key":"Status","value":"ALT_SEQ"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP002684","properties":[{"key":"ProteinId","value":"AEE32350.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"OZ408683","properties":[{"key":"ProteinId","value":"CAO7030279.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AY072212","properties":[{"key":"ProteinId","value":"AAL60033.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AY096591","properties":[{"key":"ProteinId","value":"AAM20241.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AY084581","properties":[{"key":"ProteinId","value":"AAM61146.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"RefSeq","id":"NP_564533.1","properties":[{"key":"NucleotideSequenceId","value":"NM_103774.4"}]},{"database":"AlphaFoldDB","id":"Q8VYB5","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q8VYB5","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"26526","properties":[{"key":"Interactions","value":"3"}]},{"database":"FunCoup","id":"Q8VYB5","properties":[{"key":"Number of interactors","value":"3344"}]},{"database":"STRING","id":"3702.Q8VYB5","properties":[{"key":"Description","value":"-"}]},{"database":"MEROPS","id":"M67.A04","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"3702-Q8VYB5","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"244421","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"841301","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ath:AT1G48790","properties":[{"key":"Description","value":"-"}]},{"database":"Araport","id":"AT1G48790","properties":[{"key":"Description","value":"-"}]},{"database":"TAIR","id":"AT1G48790","properties":[{"key":"GeneName","value":"AMSH1"}]},{"database":"eggNOG","id":"KOG2880","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_023304_5_1_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q8VYB5","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"GLHGQWQ","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"3640at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q8VYB5","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:Q8VYB5","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000006548","properties":[{"key":"Component","value":"Chromosome 1"}]},{"database":"ExpressionAtlas","id":"Q8VYB5","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0005768","properties":[{"key":"GoTerm","value":"C:endosome"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0016020","properties":[{"key":"GoTerm","value":"C:membrane"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0005634","properties":[{"key":"GoTerm","value":"C:nucleus"},{"key":"GoEvidenceType","value":"ISM:TAIR"}]},{"database":"GO","id":"GO:0101005","properties":[{"key":"GoTerm","value":"F:deubiquitinase activity"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0061578","properties":[{"key":"GoTerm","value":"F:K63-linked deubiquitinase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0140492","properties":[{"key":"GoTerm","value":"F:metal-dependent deubiquitinase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0032511","properties":[{"key":"GoTerm","value":"P:late endosome to vacuole transport via multivesicular body sorting pathway"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0071108","properties":[{"key":"GoTerm","value":"P:protein K48-linked deubiquitination"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0070536","properties":[{"key":"GoTerm","value":"P:protein K63-linked deubiquitination"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"CDD","id":"cd08066","properties":[{"key":"EntryName","value":"MPN_AMSH_like"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.20.58.80:FF:000020","properties":[{"key":"EntryName","value":"AMSH-like ubiquitin thioesterase 3"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.140.10:FF:000024","properties":[{"key":"EntryName","value":"AMSH-like ubiquitin thioesterase 3"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.140.10","properties":[{"key":"EntryName","value":"Cytidine Deaminase, domain 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.20.58.80","properties":[{"key":"EntryName","value":"Phosphotransferase system, lactose/cellobiose-type IIA subunit"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR000555","properties":[{"key":"EntryName","value":"JAMM/MPN+_dom"}]},{"database":"InterPro","id":"IPR037518","properties":[{"key":"EntryName","value":"MPN"}]},{"database":"InterPro","id":"IPR044098","properties":[{"key":"EntryName","value":"STAMBP/STALP-like_MPN"}]},{"database":"InterPro","id":"IPR015063","properties":[{"key":"EntryName","value":"USP8_dimer"}]},{"database":"PANTHER","id":"PTHR12947","properties":[{"key":"EntryName","value":"AMSH-LIKE PROTEASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12947:SF19","properties":[{"key":"EntryName","value":"AMSH-LIKE UBIQUITIN THIOESTERASE 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01398","properties":[{"key":"EntryName","value":"JAB"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF08969","properties":[{"key":"EntryName","value":"USP8_dimer"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00232","properties":[{"key":"EntryName","value":"JAB_MPN"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF102712","properties":[{"key":"EntryName","value":"JAB1/MPN domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF140856","properties":[{"key":"EntryName","value":"USP8 N-terminal domain-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50249","properties":[{"key":"EntryName","value":"MPN"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MGSSFETIDIATSARRIGVDNRISLKFYFRIADNILKQANIFRAEKNVIDLYVMLLRFSSLALETIPSHRDYRTSLKSNKEYLRMRLLDVLTELEKLKPVVQQRIDELYPKLKPRYNVQAHPANGSLGWSSAVKPSFNSYDHAKVRNPPGHNSGYMGSRGQQFLNAAPLEERFRKMSVNFRPNEETLSKHSILGPGGLSAQWQPPKYDTKVQYPSNIDFSPVVIPSFQQLVDSKPMITNGSNDEPEKPIVEPSVASNEKIQKNYTEELSSMISFEEPESVNENNLIRQPSPPPVLAEVQDLVPALCPEVREPECMIENSLPDESLRSESPLELHIATSMMDTFMRLAKSNTKKNLETCGILAGSLKNRKFYITALIIPKQESTSDSCQATNEEEIFEVQDKQSLFPLGWIHTHPTQSCFMSSIDVHTHYSYQIMLPEAVAIVMAPQDSSRNHGIFRLTTPGGMTVIRNCDRRGFHAHSSPEDGGPIYNTCKEVYMNPNLKFDVIDLR","length":507,"molWeight":57383,"crc64":"A60AF211A0754352","md5":"ECB872189A5B0C2883B78EF9266B7582"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"COFACTOR":1,"SUBCELLULAR LOCATION":1,"DOMAIN":1,"SIMILARITY":1,"SEQUENCE CAUTION":1},"countByFeatureType":{"Chain":1,"Domain":1,"Motif":1,"Binding site":7,"Site":1,"Sequence conflict":1},"uniParcId":"UPI00000A557A"}}