{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q8RWF4","secondaryAccessions":["Q9LSE5"],"uniProtkbId":"NIPA8_ARATH","entryAudit":{"firstPublicDate":"2014-10-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2002-06-01","entryVersion":127,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Arabidopsis thaliana","commonName":"Mouse-ear cress","taxonId":3702,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","rosids","malvids","Brassicales","Brassicaceae","Camelineae","Arabidopsis"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"value":"Probable magnesium transporter NIPA8"}}},"genes":[{"orderedLocusNames":[{"value":"At3g26670"}],"orfNames":[{"value":"MLJ15.6"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Acts as a Mg(2+) transporter. Can also transport other divalent cations such as Fe(2+), Sr(2+), Ba(2+), Mn(2+) and Co(2+) but to a much less extent than Mg(2+) (By similarity)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Homodimer"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Recruited to the cell membrane in response to low extracellular magnesium"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Multi-pass membrane protein","id":"SL-9909"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Early endosome","id":"SL-0094"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the NIPA (TC 2.A.7) family"}],"commentType":"SIMILARITY"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous gene model prediction","sequence":"BAB01731.1","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":441,"modifier":"EXACT"}},"description":"Probable magnesium transporter NIPA8","featureId":"PRO_0000430296"},{"type":"Topological domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":4,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":5,"modifier":"EXACT"},"end":{"value":25,"modifier":"EXACT"}},"description":"Helical; Name=1","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":26,"modifier":"EXACT"},"end":{"value":56,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":57,"modifier":"EXACT"},"end":{"value":77,"modifier":"EXACT"}},"description":"Helical; Name=2","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":78,"modifier":"EXACT"},"end":{"value":79,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":80,"modifier":"EXACT"},"end":{"value":100,"modifier":"EXACT"}},"description":"Helical; Name=3","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":105,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":106,"modifier":"EXACT"},"end":{"value":126,"modifier":"EXACT"}},"description":"Helical; Name=4","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":127,"modifier":"EXACT"},"end":{"value":144,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":145,"modifier":"EXACT"},"end":{"value":165,"modifier":"EXACT"}},"description":"Helical; Name=5","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":166,"modifier":"EXACT"},"end":{"value":184,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":185,"modifier":"EXACT"},"end":{"value":205,"modifier":"EXACT"}},"description":"Helical; Name=6","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":206,"modifier":"EXACT"},"end":{"value":222,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":223,"modifier":"EXACT"},"end":{"value":243,"modifier":"EXACT"}},"description":"Helical; Name=7","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":244,"modifier":"EXACT"},"end":{"value":255,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":256,"modifier":"EXACT"},"end":{"value":276,"modifier":"EXACT"}},"description":"Helical; Name=8","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":277,"modifier":"EXACT"},"end":{"value":288,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":289,"modifier":"EXACT"},"end":{"value":309,"modifier":"EXACT"}},"description":"Helical; Name=9","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":310,"modifier":"EXACT"},"end":{"value":441,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Region","location":{"start":{"value":313,"modifier":"EXACT"},"end":{"value":347,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":320,"modifier":"EXACT"},"end":{"value":330,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":334,"modifier":"EXACT"},"end":{"value":347,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]}],"keywords":[{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0967","category":"Cellular component","name":"Endosome"},{"id":"KW-0406","category":"Biological process","name":"Ion transport"},{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"10819329","citationType":"journal article","authors":["Sato S.","Nakamura Y.","Kaneko T.","Katoh T.","Asamizu E.","Tabata S."],"citationCrossReferences":[{"database":"PubMed","id":"10819329"},{"database":"DOI","id":"10.1093/dnares/7.2.131"}],"title":"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones.","publicationDate":"2000","journal":"DNA Res.","firstPage":"131","lastPage":"135","volume":"7"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"27862469","citationType":"journal article","authors":["Cheng C.Y.","Krishnakumar V.","Chan A.P.","Thibaud-Nissen F.","Schobel S.","Town C.D."],"citationCrossReferences":[{"database":"PubMed","id":"27862469"},{"database":"DOI","id":"10.1111/tpj.13415"}],"title":"Araport11: a complete reannotation of the Arabidopsis thaliana reference genome.","publicationDate":"2017","journal":"Plant J.","firstPage":"789","lastPage":"804","volume":"89"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"CI-CI4AM1G71UC0S","citationType":"submission","authoringGroup":["The Arabidopsis Information Resource (TAIR)"],"title":"Community-consensus reference genome of Arabidopsis thaliana Col-0.","publicationDate":"FEB-2026","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"14593172","citationType":"journal article","authors":["Yamada K.","Lim J.","Dale J.M.","Chen H.","Shinn P.","Palm C.J.","Southwick A.M.","Wu H.C.","Kim C.J.","Nguyen M.","Pham P.K.","Cheuk R.F.","Karlin-Newmann G.","Liu S.X.","Lam B.","Sakano H.","Wu T.","Yu G.","Miranda M.","Quach H.L.","Tripp M.","Chang C.H.","Lee J.M.","Toriumi M.J.","Chan M.M.","Tang C.C.","Onodera C.S.","Deng J.M.","Akiyama K.","Ansari Y.","Arakawa T.","Banh J.","Banno F.","Bowser L.","Brooks S.Y.","Carninci P.","Chao Q.","Choy N.","Enju A.","Goldsmith A.D.","Gurjal M.","Hansen N.F.","Hayashizaki Y.","Johnson-Hopson C.","Hsuan V.W.","Iida K.","Karnes M.","Khan S.","Koesema E.","Ishida J.","Jiang P.X.","Jones T.","Kawai J.","Kamiya A.","Meyers C.","Nakajima M.","Narusaka M.","Seki M.","Sakurai T.","Satou M.","Tamse R.","Vaysberg M.","Wallender E.K.","Wong C.","Yamamura Y.","Yuan S.","Shinozaki K.","Davis R.W.","Theologis A.","Ecker J.R."],"citationCrossReferences":[{"database":"PubMed","id":"14593172"},{"database":"DOI","id":"10.1126/science.1088305"}],"title":"Empirical analysis of transcriptional activity in the Arabidopsis genome.","publicationDate":"2003","journal":"Science","firstPage":"842","lastPage":"846","volume":"302"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"CI-1ND7IVR87IF4I","citationType":"submission","authors":["Totoki Y.","Seki M.","Ishida J.","Nakajima M.","Enju A.","Kamiya A.","Narusaka M.","Shin-i T.","Nakagawa M.","Sakamoto N.","Oishi K.","Kohara Y.","Kobayashi M.","Toyoda A.","Sakaki Y.","Sakurai T.","Iida K.","Akiyama K.","Satou M.","Toyoda T.","Konagaya A.","Carninci P.","Kawai J.","Hayashizaki Y.","Shinozaki K."],"title":"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.","publicationDate":"JUL-2006","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB026648","properties":[{"key":"ProteinId","value":"BAB01731.1"},{"key":"Status","value":"ALT_SEQ"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP002686","properties":[{"key":"ProteinId","value":"AEE77193.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP002686","properties":[{"key":"ProteinId","value":"AEE77194.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP002686","properties":[{"key":"ProteinId","value":"AEE77195.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP002686","properties":[{"key":"ProteinId","value":"ANM64303.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"OZ408685","properties":[{"key":"ProteinId","value":"CAO7042477.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AY093129","properties":[{"key":"ProteinId","value":"AAM13128.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BT006587","properties":[{"key":"ProteinId","value":"AAP31931.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK226900","properties":[{"key":"ProteinId","value":"BAE98977.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"RefSeq","id":"NP_001326342.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001338815.1"}]},{"database":"RefSeq","id":"NP_189301.1","properties":[{"key":"NucleotideSequenceId","value":"NM_113578.4"}]},{"database":"RefSeq","id":"NP_850634.1","properties":[{"key":"NucleotideSequenceId","value":"NM_180303.4"}]},{"database":"RefSeq","id":"NP_974367.1","properties":[{"key":"NucleotideSequenceId","value":"NM_202638.2"}]},{"database":"AlphaFoldDB","id":"Q8RWF4","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q8RWF4","properties":[{"key":"Number of interactors","value":"330"}]},{"database":"PaxDb","id":"3702-Q8RWF4","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"251173","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"822279","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ath:AT3G26670","properties":[{"key":"Description","value":"-"}]},{"database":"Araport","id":"AT3G26670","properties":[{"key":"Description","value":"-"}]},{"database":"TAIR","id":"AT3G26670","properties":[{"key":"GeneName","value":"-"}]},{"database":"eggNOG","id":"KOG2922","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_054115_0_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q8RWF4","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"YYGPLAC","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"165382at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q8RWF4","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:Q8RWF4","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000006548","properties":[{"key":"Component","value":"Chromosome 3"}]},{"database":"ExpressionAtlas","id":"Q8RWF4","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0009507","properties":[{"key":"GoTerm","value":"C:chloroplast"},{"key":"GoEvidenceType","value":"ISM:TAIR"}]},{"database":"GO","id":"GO:0005769","properties":[{"key":"GoTerm","value":"C:early endosome"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"PubMed","id":"17166836"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"PubMed","id":"17166836"}]},{"database":"GO","id":"GO:0015095","properties":[{"key":"GoTerm","value":"F:magnesium ion transmembrane transporter activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0015693","properties":[{"key":"GoTerm","value":"P:magnesium ion transport"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"PubMed","id":"17166836"}]},{"database":"InterPro","id":"IPR037185","properties":[{"key":"EntryName","value":"EmrE-like"}]},{"database":"InterPro","id":"IPR008521","properties":[{"key":"EntryName","value":"Mg_trans_NIPA"}]},{"database":"PANTHER","id":"PTHR12570","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12570:SF9","properties":[{"key":"EntryName","value":"MAGNESIUM TRANSPORTER NIPA8-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF05653","properties":[{"key":"EntryName","value":"Mg_trans_NIPA"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF103481","properties":[{"key":"EntryName","value":"Multidrug resistance efflux transporter EmrE"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MGEWVIGAFINIFGSVAINFGTNLLKLGHNERERLALQDGGGKMPLKPIIHNQTWRVGILVFLLGNCLNFISFGYAAQSLLAALGSIQFVSNIAFAYVVLNKMVTVKVLVATAFIVLGNVFLVAFGNHQSPVFTPEQLAEKYSNVTFLVYCGILILIVAVHHFLYRKGEVLISTPGQEISSYWKMLLPFSYAVVSGAIGSCSVLFAKSLSNLLRLAMSSSYQLHSWFTYSMLLLFLSTAGFWMTRLNEGLSLYDAILIVPMFQIAWTFFSICTGCIYFQEFQVFDALRTTMFILGMMCVFIGISLLAPDDTRGNETKDNSSSLDSIVSSSVPTEEDRLIPQSSEDGHSKDTRVVVQGMYMKAADLIAKTKTACLAALGFGEDSINASAILVMPMVSSKITGFRGNGLERAKILSMRGSGWSKLAMEEEGTRMLEKTISSKA","length":441,"molWeight":48409,"crc64":"43B04EEB89C9024F","md5":"DAE0C0F8D1F7AB23FAF725814FEE4449"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"SEQUENCE CAUTION":1},"countByFeatureType":{"Chain":1,"Topological domain":10,"Transmembrane":9,"Region":1,"Compositional bias":2},"uniParcId":"UPI00000A68B9"}}