{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q8R040","secondaryAccessions":["Q80XY3","Q811B7","Q9CPX1"],"uniProtkbId":"RPP21_MOUSE","entryAudit":{"firstPublicDate":"2004-07-05","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2004-07-05","entryVersion":145,"sequenceVersion":2},"annotationScore":3.0,"organism":{"scientificName":"Mus musculus","commonName":"Mouse","taxonId":10090,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Glires","Rodentia","Myomorpha","Muroidea","Muridae","Murinae","Mus","Mus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Ribonuclease P protein subunit p21"},"shortNames":[{"value":"RNaseP protein p21"}]},"alternativeNames":[{"fullName":{"value":"Ribonucleoprotein V"}}]},"genes":[{"geneName":{"value":"Rpp21"},"synonyms":[{"value":"Cat60"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9H633"}],"value":"Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9H633"}],"value":"RNase P consists of a catalytic RNA moiety and about 10 protein subunits; POP1, POP4, POP5, POP7, RPP14, RPP21, RPP25, RPP30, RPP38 and RPP40. Within the RNase P complex, POP1, POP7 and RPP25 form the 'finger' subcomplex, POP5, RPP14, RPP40 and homodimeric RPP30 form the 'palm' subcomplex, and RPP21, POP4 and RPP38 form the 'wrist' subcomplex. All subunits of the RNase P complex interact with the catalytic RNA"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9H633"}],"value":"Nucleus, nucleolus","id":"SL-0188"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the eukaryotic/archaeal RNase P protein component 4 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Initiator methionine","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"Removed","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9H633"}]},{"type":"Chain","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":150,"modifier":"EXACT"}},"description":"Ribonuclease P protein subunit p21","featureId":"PRO_0000153846"},{"type":"Region","location":{"start":{"value":121,"modifier":"EXACT"},"end":{"value":150,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":140,"modifier":"EXACT"},"end":{"value":150,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Binding site","location":{"start":{"value":62,"modifier":"EXACT"},"end":{"value":62,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105"}},{"type":"Binding site","location":{"start":{"value":65,"modifier":"EXACT"},"end":{"value":65,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105"}},{"type":"Binding site","location":{"start":{"value":92,"modifier":"EXACT"},"end":{"value":92,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105"}},{"type":"Binding site","location":{"start":{"value":95,"modifier":"EXACT"},"end":{"value":95,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105"}},{"type":"Modified residue","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":2,"modifier":"EXACT"}},"description":"N-acetylalanine","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9H633"}]},{"type":"Sequence conflict","location":{"start":{"value":18,"modifier":"EXACT"},"end":{"value":18,"modifier":"EXACT"}},"description":"in Ref. 1; AAP03887","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"Y","alternativeSequences":["C"]}},{"type":"Sequence conflict","location":{"start":{"value":47,"modifier":"EXACT"},"end":{"value":53,"modifier":"EXACT"}},"description":"in Ref. 2; CAD44293","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"KRLVLRQ","alternativeSequences":["NAGPPA"]}},{"type":"Sequence conflict","location":{"start":{"value":141,"modifier":"EXACT"},"end":{"value":141,"modifier":"EXACT"}},"description":"in Ref. 4; AAH28491","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"I","alternativeSequences":["M"]}}],"keywords":[{"id":"KW-0007","category":"PTM","name":"Acetylation"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0539","category":"Cellular component","name":"Nucleus"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0819","category":"Biological process","name":"tRNA processing"},{"id":"KW-0862","category":"Ligand","name":"Zinc"}],"references":[{"referenceNumber":1,"citation":{"id":"12671000","citationType":"journal article","authors":["Takada T.","Kumanovics A.","Amadou C.","Yoshino M.","Jones E.P.","Athanasiou M.","Evans G.A.","Lindahl K.F."],"citationCrossReferences":[{"database":"PubMed","id":"12671000"},{"database":"DOI","id":"10.1101/gr.975303"}],"title":"Species-specific class I gene expansions formed the telomeric 1 mb of the mouse major histocompatibility complex.","publicationDate":"2003","journal":"Genome Res.","firstPage":"589","lastPage":"600","volume":"13"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]"],"referenceComments":[{"value":"129/SvJ","type":"STRAIN"},{"value":"Testis","type":"TISSUE"}]},{"referenceNumber":2,"citation":{"id":"CI-ETL311KSJLQQL","citationType":"submission","authors":["Raha-Chowdhury R.","Andrews S.R.","Gruen J.R.","Weissman S.M."],"title":"Genes from major histocompatibility complex (MHC) class I region from HLA-C to HLA-A.","publicationDate":"AUG-2002","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]"],"referenceComments":[{"value":"C57BL/6J","type":"STRAIN"},{"value":"Spleen","type":"TISSUE"}]},{"referenceNumber":3,"citation":{"id":"16141072","citationType":"journal article","authors":["Carninci P.","Kasukawa T.","Katayama S.","Gough J.","Frith M.C.","Maeda N.","Oyama R.","Ravasi T.","Lenhard B.","Wells C.","Kodzius R.","Shimokawa K.","Bajic V.B.","Brenner S.E.","Batalov S.","Forrest A.R.","Zavolan M.","Davis M.J.","Wilming L.G.","Aidinis V.","Allen J.E.","Ambesi-Impiombato A.","Apweiler R.","Aturaliya R.N.","Bailey T.L.","Bansal M.","Baxter L.","Beisel K.W.","Bersano T.","Bono H.","Chalk A.M.","Chiu K.P.","Choudhary V.","Christoffels A.","Clutterbuck D.R.","Crowe M.L.","Dalla E.","Dalrymple B.P.","de Bono B.","Della Gatta G.","di Bernardo D.","Down T.","Engstrom P.","Fagiolini M.","Faulkner G.","Fletcher C.F.","Fukushima T.","Furuno M.","Futaki S.","Gariboldi M.","Georgii-Hemming P.","Gingeras T.R.","Gojobori T.","Green R.E.","Gustincich S.","Harbers M.","Hayashi Y.","Hensch T.K.","Hirokawa N.","Hill D.","Huminiecki L.","Iacono M.","Ikeo K.","Iwama A.","Ishikawa T.","Jakt M.","Kanapin A.","Katoh M.","Kawasawa Y.","Kelso J.","Kitamura H.","Kitano H.","Kollias G.","Krishnan S.P.","Kruger A.","Kummerfeld S.K.","Kurochkin I.V.","Lareau L.F.","Lazarevic D.","Lipovich L.","Liu J.","Liuni S.","McWilliam S.","Madan Babu M.","Madera M.","Marchionni L.","Matsuda H.","Matsuzawa S.","Miki H.","Mignone F.","Miyake S.","Morris K.","Mottagui-Tabar S.","Mulder N.","Nakano N.","Nakauchi H.","Ng P.","Nilsson R.","Nishiguchi S.","Nishikawa S.","Nori F.","Ohara O.","Okazaki Y.","Orlando V.","Pang K.C.","Pavan W.J.","Pavesi G.","Pesole G.","Petrovsky N.","Piazza S.","Reed J.","Reid J.F.","Ring B.Z.","Ringwald M.","Rost B.","Ruan Y.","Salzberg S.L.","Sandelin A.","Schneider C.","Schoenbach C.","Sekiguchi K.","Semple C.A.","Seno S.","Sessa L.","Sheng Y.","Shibata Y.","Shimada H.","Shimada K.","Silva D.","Sinclair B.","Sperling S.","Stupka E.","Sugiura K.","Sultana R.","Takenaka Y.","Taki K.","Tammoja K.","Tan S.L.","Tang S.","Taylor M.S.","Tegner J.","Teichmann S.A.","Ueda H.R.","van Nimwegen E.","Verardo R.","Wei C.L.","Yagi K.","Yamanishi H.","Zabarovsky E.","Zhu S.","Zimmer A.","Hide W.","Bult C.","Grimmond S.M.","Teasdale R.D.","Liu E.T.","Brusic V.","Quackenbush J.","Wahlestedt C.","Mattick J.S.","Hume D.A.","Kai C.","Sasaki D.","Tomaru Y.","Fukuda S.","Kanamori-Katayama M.","Suzuki M.","Aoki J.","Arakawa T.","Iida J.","Imamura K.","Itoh M.","Kato T.","Kawaji H.","Kawagashira N.","Kawashima T.","Kojima M.","Kondo S.","Konno H.","Nakano K.","Ninomiya N.","Nishio T.","Okada M.","Plessy C.","Shibata K.","Shiraki T.","Suzuki S.","Tagami M.","Waki K.","Watahiki A.","Okamura-Oho Y.","Suzuki H.","Kawai J.","Hayashizaki Y."],"citationCrossReferences":[{"database":"PubMed","id":"16141072"},{"database":"DOI","id":"10.1126/science.1112014"}],"title":"The transcriptional landscape of the mammalian genome.","publicationDate":"2005","journal":"Science","firstPage":"1559","lastPage":"1563","volume":"309"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"C57BL/6J","type":"STRAIN"},{"value":"Embryo","type":"TISSUE"},{"value":"Kidney","type":"TISSUE"}]},{"referenceNumber":4,"citation":{"id":"15489334","citationType":"journal article","authoringGroup":["The MGC Project Team"],"citationCrossReferences":[{"database":"PubMed","id":"15489334"},{"database":"DOI","id":"10.1101/gr.2596504"}],"title":"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).","publicationDate":"2004","journal":"Genome Res.","firstPage":"2121","lastPage":"2127","volume":"14"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"Uterus","type":"TISSUE"}]},{"referenceNumber":5,"citation":{"id":"21183079","citationType":"journal article","authors":["Huttlin E.L.","Jedrychowski M.P.","Elias J.E.","Goswami T.","Rad R.","Beausoleil S.A.","Villen J.","Haas W.","Sowa M.E.","Gygi S.P."],"citationCrossReferences":[{"database":"PubMed","id":"21183079"},{"database":"DOI","id":"10.1016/j.cell.2010.12.001"}],"title":"A tissue-specific atlas of mouse protein phosphorylation and expression.","publicationDate":"2010","journal":"Cell","firstPage":"1174","lastPage":"1189","volume":"143"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"Spleen","type":"TISSUE"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AY251016","properties":[{"key":"ProteinId","value":"AAP03887.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AJ504717","properties":[{"key":"ProteinId","value":"CAD44293.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK002780","properties":[{"key":"ProteinId","value":"BAB22353.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK003455","properties":[{"key":"ProteinId","value":"BAB22800.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC028491","properties":[{"key":"ProteinId","value":"AAH28491.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"CCDS","id":"CCDS28719.1","properties":[{"key":"Description","value":"-"}]},{"database":"RefSeq","id":"NP_080584.1","properties":[{"key":"NucleotideSequenceId","value":"NM_026308.2"}]},{"database":"AlphaFoldDB","id":"Q8R040","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q8R040","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"212359","properties":[{"key":"Interactions","value":"1"}]},{"database":"FunCoup","id":"Q8R040","properties":[{"key":"Number of interactors","value":"18"}]},{"database":"STRING","id":"10090.ENSMUSP00000025319","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"Q8R040","properties":[{"key":"Description","value":"-"}]},{"database":"PhosphoSitePlus","id":"Q8R040","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"10090-ENSMUSP00000025319","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"Q8R040","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"262703","properties":[{"key":"Description","value":"-"}]},{"database":"Pumba","id":"Q8R040","properties":[{"key":"Description","value":"-"}]},{"database":"Antibodypedia","id":"34965","properties":[{"key":"antibodies","value":"53 antibodies from 14 providers"}]},{"database":"DNASU","id":"67676","properties":[{"key":"Description","value":"-"}]},{"database":"Ensembl","id":"ENSMUST00000025319.7","properties":[{"key":"ProteinId","value":"ENSMUSP00000025319.7"},{"key":"GeneId","value":"ENSMUSG00000024446.14"}]},{"database":"GeneID","id":"67676","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"mmu:67676","properties":[{"key":"Description","value":"-"}]},{"database":"UCSC","id":"uc008ckq.1","properties":[{"key":"OrganismName","value":"mouse"}]},{"database":"AGR","id":"MGI:1914926","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"79897","properties":[{"key":"Description","value":"-"}]},{"database":"MGI","id":"MGI:1914926","properties":[{"key":"GeneName","value":"Rpp21"}]},{"database":"VEuPathDB","id":"HostDB:ENSMUSG00000024446","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG4394","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"GeneTree","id":"ENSGT00390000003020","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_079140_2_1_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q8R040","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"DPKHLLW","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"128536at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q8R040","properties":[{"key":"Description","value":"-"}]},{"database":"Reactome","id":"R-MMU-6791226","properties":[{"key":"PathwayName","value":"Major pathway of rRNA processing in the nucleolus and cytosol"}]},{"database":"BioGRID-ORCS","id":"67676","properties":[{"key":"hits","value":"31 hits in 80 CRISPR screens"}]},{"database":"PRO","id":"PR:Q8R040","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000589","properties":[{"key":"Component","value":"Chromosome 17"}]},{"database":"RNAct","id":"Q8R040","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"Bgee","id":"ENSMUSG00000024446","properties":[{"key":"ExpressionPatterns","value":"Expressed in embryonic cell in blastocyst and 196 other cell types or tissues"}]},{"database":"ExpressionAtlas","id":"Q8R040","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0030681","properties":[{"key":"GoTerm","value":"C:multimeric ribonuclease P complex"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0005655","properties":[{"key":"GoTerm","value":"C:nucleolar ribonuclease P complex"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0004526","properties":[{"key":"GoTerm","value":"F:ribonuclease P activity"},{"key":"GoEvidenceType","value":"IEA:Ensembl"}]},{"database":"GO","id":"GO:0033204","properties":[{"key":"GoTerm","value":"F:ribonuclease P RNA binding"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0009410","properties":[{"key":"GoTerm","value":"P:response to xenobiotic stimulus"},{"key":"GoEvidenceType","value":"IEA:Ensembl"}]},{"database":"GO","id":"GO:0001682","properties":[{"key":"GoTerm","value":"P:tRNA 5'-leader removal"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0008033","properties":[{"key":"GoTerm","value":"P:tRNA processing"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"Gene3D","id":"6.20.50.20","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR007175","properties":[{"key":"EntryName","value":"Rpr2/Snm1/Rpp21"}]},{"database":"PANTHER","id":"PTHR14742:SF0","properties":[{"key":"EntryName","value":"RIBONUCLEASE P PROTEIN SUBUNIT P21"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR14742","properties":[{"key":"EntryName","value":"RIBONUCLEASE P SUBUNIT P21"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04032","properties":[{"key":"EntryName","value":"Rpr2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAGPVKDREAFQRLSFLYQAAHCVLSQNPENQALARFYCHTEKTIAKRLVLRQDPSVKRTLCRSCSSLLIPGLTCTQRQRRRKGQRWTVQTCLTCQRSQRFLNDPKHLLWGDRPEAQLENQADINPSEPLPNIADLPKENIQTQALNTSE","length":150,"molWeight":17243,"crc64":"EDB5C52A6F2FF519","md5":"1E4A1665D0EF786BB977D13812F1EFDB"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Initiator methionine":1,"Chain":1,"Region":1,"Compositional bias":1,"Binding site":4,"Modified residue":1,"Sequence conflict":3},"uniParcId":"UPI0000022358"}}