{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q8R000","uniProtkbId":"OSTA_MOUSE","entryAudit":{"firstPublicDate":"2008-04-29","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2002-06-01","entryVersion":146,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Mus musculus","commonName":"Mouse","taxonId":10090,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Glires","Rodentia","Myomorpha","Muroidea","Muridae","Murinae","Mus","Mus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"22535958"}],"value":"Organic solute transporter subunit alpha"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"17650074"}],"value":"OST-alpha"}]},"alternativeNames":[{"fullName":{"value":"Solute carrier family 51 subunit alpha"}}]},"genes":[{"geneName":{"value":"Slc51a"},"synonyms":[{"value":"Osta"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q86UW1"},{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q90YM5"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15563450"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16317684"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17650074"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18292224"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22535958"}],"value":"Essential component of the Ost-alpha/Ost-beta complex, a heterotetramer that acts as the intestinal basolateral transporter responsible for bile acid export from enterocytes into portal blood (PubMed:15563450, PubMed:16317684, PubMed:17650074, PubMed:18292224, PubMed:22535958). The OST-alpha/OST-beta complex efficiently transports the major species of bile acids (taurocholate) (PubMed:16317684, PubMed:17650074, PubMed:18292224, PubMed:22535958). Taurine conjugates are transported more efficiently across the basolateral membrane than glycine-conjugated bile acids (PubMed:16317684). In the complex, SLC51A/OST-alpha adopts a G protein-coupled receptor fold and constitutes a bile acid recognition site (By similarity). Can also transport steroids such as estrone 3-sulfate and dehydroepiandrosterone 3-sulfate, therefore playing a role in the enterohepatic circulation of sterols (By similarity). Able to transport eicosanoids such as prostaglandin E2 (By similarity)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"taurocholate(out) = taurocholate(in)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:71703"},{"database":"ChEBI","id":"CHEBI:36257"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16317684"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17650074"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22535958"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"18292224"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"tauroursodeoxycholate(out) = tauroursodeoxycholate(in)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:71843"},{"database":"ChEBI","id":"CHEBI:132028"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16317684"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"glycoursodeoxycholate(out) = glycoursodeoxycholate(in)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:71847"},{"database":"ChEBI","id":"CHEBI:132030"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16317684"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"glycocholate(out) = glycocholate(in)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:71851"},{"database":"ChEBI","id":"CHEBI:29746"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16317684"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"taurochenodeoxycholate(out) = taurochenodeoxycholate(in)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:71855"},{"database":"ChEBI","id":"CHEBI:9407"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16317684"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"glycochenodeoxycholate(out) = glycochenodeoxycholate(in)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:71859"},{"database":"ChEBI","id":"CHEBI:36252"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16317684"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"taurodeoxycholate(out) = taurodeoxycholate(in)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:71863"},{"database":"ChEBI","id":"CHEBI:36261"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16317684"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"glycodeoxycholate(out) = glycodeoxycholate(in)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:71867"},{"database":"ChEBI","id":"CHEBI:82982"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16317684"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"prostaglandin E2(out) = prostaglandin E2(in)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:50984"},{"database":"ChEBI","id":"CHEBI:606564"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q90YM5"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"estrone 3-sulfate(out) = estrone 3-sulfate(in)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:71835"},{"database":"ChEBI","id":"CHEBI:60050"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q86UW1"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"dehydroepiandrosterone 3-sulfate(out) = dehydroepiandrosterone 3-sulfate(in)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:71839"},{"database":"ChEBI","id":"CHEBI:57905"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q86UW1"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17650074"}],"value":"Heterotetramer; the Ost-alpha/Ost-beta complex forms a symmetric dimer of heterodimers of SLC51A and SLC51B"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17650074"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22535958"}],"value":"Transported from the endoplasmic reticulum to the plasma membrane upon interacting with SLC51B (PubMed:17650074). Mainly restricted to the lateral and basal membranes of ileal enterocytes (PubMed:17650074, PubMed:22535958)"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15563450"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17650074"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22535958"}],"value":"Basolateral cell membrane","id":"SL-0026"},"topology":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q86UW1"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15563450"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16317684"}],"value":"Present at high levels in ileum. In ileum, it is restricted to the apical domain on the mature villus enterocytes with little detectable expression in the goblet cells or crypt enterocytes (at protein level). Expressed in kidney but not in heart, brain, liver, spleen, embryo, lung, thymus, ovary nor testis"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16357058"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16628672"}],"value":"Positively regulated via the bile acid-activated nuclear receptor farnesoid X receptor (NR1H4/FXR). Up-regulated in mice lacking Mrp4, but it is unable to compensate for the absence of Mrp4"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q86UW1"}],"value":"In the complex, two SLC51A/OST-alpha units form the homodimeric core, with two SLC51B/OST-beta units bound to the periphery. SLC51A/OST-alpha adopts a G protein-coupled receptor fold and contains a unique cysteine-rich loop with seven palmitoylation sites to constitute a bile acid recognition site. A positive cavity in SLC51A/OST-alpha connects the bile acid site and facilitates the transmembrane translocation of bile acids through SLC51B/OST-beta"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q86UW1"}],"value":"The cysteine-rich loop is highly palmitoylated"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q86UW1"}],"value":"Palmitoylation of the cysteine-rich loop is required to form a lateral substrate-binding groove for recognition of bile acids"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"15563450"}],"value":"N-glycosylated"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18292224"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24381083"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25626867"}],"value":"Mice are physically indistinguishable from wild-type mice but display strongly reduced transileal transport of taurocholate (PubMed:18292224). Moreover, the bile acid pool size is significantly reduced, but fecal bile acid excretion is not elevated (PubMed:18292224). Mice are resistant to age-related weight gain, body fat accumulation, and lipid accumulation in liver and muscle (PubMed:24381083). Mutant male mice live slightly longer than wild-type mice (PubMed:24381083). Mutant mice are however not protected from western diet-induced weight gain (PubMed:25626867)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the OST-alpha family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":340,"modifier":"EXACT"}},"description":"Organic solute transporter subunit alpha","featureId":"PRO_0000331544"},{"type":"Topological domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q86UW2"}]},{"type":"Transmembrane","location":{"start":{"value":49,"modifier":"EXACT"},"end":{"value":80,"modifier":"EXACT"}},"description":"Helical; Name=TM1","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q86UW1"}]},{"type":"Topological 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