{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q8NFU1","secondaryAccessions":["Q53YQ8","Q9NXP0"],"uniProtkbId":"BEST2_HUMAN","entryAudit":{"firstPublicDate":"2003-11-21","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2002-10-01","entryVersion":171,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Homo sapiens","commonName":"Human","taxonId":9606,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Primates","Haplorrhini","Catarrhini","Hominidae","Homo"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Bestrophin-2a"}},"alternativeNames":[{"fullName":{"value":"Vitelliform macular dystrophy 2-like protein 1"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000312","source":"HGNC","id":"HGNC:17107"}],"value":"BEST2"},"synonyms":[{"value":"VMD2L1"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"E1BF86"},{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8BGM5"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11904445"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18400985"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32251414"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35789156"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36289327"}],"value":"Ligand-gated anion channel that allows the movement of anions across cell membranes when activated by calcium (Ca2+) (PubMed:11904445, PubMed:18400985, PubMed:32251414, PubMed:35789156, PubMed:36289327). Transports a large specter of anions, namely mediates the movement of chloride, L-glutamate and iodide (PubMed:11904445, PubMed:18400985, PubMed:32251414, PubMed:35789156, PubMed:36289327). Calcium-binding triggers the dilation of the aperture, but calcium-dependent gating is only effective when the size of the passing anion is bigger than the closed aperture (By similarity). Mediates the calcium-activated hydrogencarbonate movement and participates in colonic hydrogencarbonate secretion concomitant with mucin secretion (By similarity). In non-pigmented epithelium (NPE), mediates the efflux of intracellular L-glutamate; binding of intracellular L-glutamate activates and open both the neck and the aperture of the channel, leading to L-glutamate exit promoting chloride influx movement from the extracellular side in trans (PubMed:36289327). Also exhibits a directional permeability for intracellular glutamine, in a similar manner as for L-glutamate (PubMed:36289327)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"chloride(in) = chloride(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:29823"},{"database":"ChEBI","id":"CHEBI:17996"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11904445"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18400985"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32251414"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35789156"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36289327"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"hydrogencarbonate(in) = hydrogencarbonate(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:28695"},{"database":"ChEBI","id":"CHEBI:17544"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11904445"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18400985"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"L-glutamate(out) = L-glutamate(in)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:66336"},{"database":"ChEBI","id":"CHEBI:29985"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36289327"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"iodide(out) = iodide(in)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:66324"},{"database":"ChEBI","id":"CHEBI:16382"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"E1BF86"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"L-glutamine(out) = L-glutamine(in)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:73419"},{"database":"ChEBI","id":"CHEBI:58359"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36289327"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36289327"}],"value":"Chloride channel activity is allosterically inhibited by GLUL/glutamine synthase (GS) which affects the gating at the aperture in the absence of intracellular glutamate (PubMed:36289327). Inhibitory effect of GLUL is relieved upon increasing of L-glutamate intracellular level (PubMed:36289327)"}],"commentType":"ACTIVITY REGULATION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35789156"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36289327"}],"value":"Pentamer (PubMed:35789156). Interacts with GLUL; this interaction tethers a fraction of GLUL to the membrane, causing a decrease of cytosolic glutamine synthase (GS) activity and inhibits the chloride channel activity of BEST2 by affecting the gating at the aperture in the absence of intracellular glutamate (PubMed:36289327)"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"Q8NFU1","intActId":"EBI-19947314"},"interactantTwo":{"uniProtKBAccession":"P78369","geneName":"CLDN10","intActId":"EBI-13372810"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q8NFU1","intActId":"EBI-19947314"},"interactantTwo":{"uniProtKBAccession":"P21964","geneName":"COMT","intActId":"EBI-372265"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q8NFU1","intActId":"EBI-19947314"},"interactantTwo":{"uniProtKBAccession":"P49447","geneName":"CYB561","intActId":"EBI-8646596"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q8NFU1","intActId":"EBI-19947314"},"interactantTwo":{"uniProtKBAccession":"Q96LL9","geneName":"DNAJC30","intActId":"EBI-8639143"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q8NFU1","intActId":"EBI-19947314"},"interactantTwo":{"uniProtKBAccession":"P52803","geneName":"EFNA5","intActId":"EBI-1753674"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q8NFU1","intActId":"EBI-19947314"},"interactantTwo":{"uniProtKBAccession":"Q14802-3","geneName":"FXYD3","intActId":"EBI-12175685"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q8NFU1","intActId":"EBI-19947314"},"interactantTwo":{"uniProtKBAccession":"O00155","geneName":"GPR25","intActId":"EBI-10178951"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q8NFU1","intActId":"EBI-19947314"},"interactantTwo":{"uniProtKBAccession":"Q13021","geneName":"MALL","intActId":"EBI-750078"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q8NFU1","intActId":"EBI-19947314"},"interactantTwo":{"uniProtKBAccession":"Q6N075","geneName":"MFSD5","intActId":"EBI-3920969"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q8NFU1","intActId":"EBI-19947314"},"interactantTwo":{"uniProtKBAccession":"P54315","geneName":"PNLIPRP1","intActId":"EBI-8652812"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q8NFU1","intActId":"EBI-19947314"},"interactantTwo":{"uniProtKBAccession":"Q96AA3","geneName":"RFT1","intActId":"EBI-6269616"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q8NFU1","intActId":"EBI-19947314"},"interactantTwo":{"uniProtKBAccession":"Q8N2U9","geneName":"SLC66A2","intActId":"EBI-3907610"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q8NFU1","intActId":"EBI-19947314"},"interactantTwo":{"uniProtKBAccession":"Q5BJH2-2","geneName":"TMEM128","intActId":"EBI-10694905"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q8NFU1","intActId":"EBI-19947314"},"interactantTwo":{"uniProtKBAccession":"Q9H2L4","geneName":"TMEM60","intActId":"EBI-2852148"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q8NFU1","intActId":"EBI-19947314"},"interactantTwo":{"uniProtKBAccession":"Q9H1C4","geneName":"UNC93B1","intActId":"EBI-4401271"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q8NFU1","intActId":"EBI-19947314"},"interactantTwo":{"uniProtKBAccession":"O75841","geneName":"UPK1B","intActId":"EBI-12237619"},"numberOfExperiments":3,"organismDiffer":false}]},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"E1BF86"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35789156"}],"value":"Multi-pass membrane protein","id":"SL-9909"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20407206"}],"value":"Basolateral cell membrane","id":"SL-0026"},"topology":{"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12032738"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20407206"}],"value":"Mainly confined to the retinal pigment epithelium (PubMed:12032738). Expressed in colon (PubMed:12032738, PubMed:20407206)"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35789156"}],"value":"The C-terminal auto-inhibitory segment (AS) modulates the open/closed conformation of the channel and determines paralog specificity among bestrophins (PubMed:35789156). In a closed conformation, the C-terminal auto-inhibitory segment constricts the channel concentrically by wrapping around the channel periphery in an inter-protomer manner (PubMed:35789156)"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the anion channel-forming bestrophin (TC 1.A.46) family. Calcium-sensitive chloride channel subfamily"}],"commentType":"SIMILARITY"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"BAA90970.1","note":"Truncated N-terminus.","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":509,"modifier":"EXACT"}},"description":"Bestrophin-2a","featureId":"PRO_0000143118"},{"type":"Topological domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":31,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O76090"}]},{"type":"Transmembrane","location":{"start":{"value":32,"modifier":"EXACT"},"end":{"value":51,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35789156"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1E"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1F"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1G"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1H"}]},{"type":"Topological domain","location":{"start":{"value":52,"modifier":"EXACT"},"end":{"value":60,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Transmembrane","location":{"start":{"value":61,"modifier":"EXACT"},"end":{"value":82,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35789156"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1E"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1F"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1G"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1H"}]},{"type":"Topological domain","location":{"start":{"value":83,"modifier":"EXACT"},"end":{"value":238,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Transmembrane","location":{"start":{"value":239,"modifier":"EXACT"},"end":{"value":255,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35789156"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1E"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1F"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1G"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1H"}]},{"type":"Topological domain","location":{"start":{"value":256,"modifier":"EXACT"},"end":{"value":274,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Transmembrane","location":{"start":{"value":275,"modifier":"EXACT"},"end":{"value":288,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35789156"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1E"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1F"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1G"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1H"}]},{"type":"Topological domain","location":{"start":{"value":289,"modifier":"EXACT"},"end":{"value":509,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O76090"}]},{"type":"Region","location":{"start":{"value":454,"modifier":"EXACT"},"end":{"value":509,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":457,"modifier":"EXACT"},"end":{"value":468,"modifier":"EXACT"}},"description":"Pro residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":489,"modifier":"EXACT"},"end":{"value":498,"modifier":"EXACT"}},"description":"Pro residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Binding site","location":{"start":{"value":10,"modifier":"EXACT"},"end":{"value":10,"modifier":"EXACT"}},"description":"in other chain","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29108"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35789156"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1E"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1F"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1G"}],"ligand":{"name":"Ca(2+)","id":"ChEBI:CHEBI:29108","note":"ligand shared between two neighboring subunits"}},{"type":"Binding site","location":{"start":{"value":293,"modifier":"EXACT"},"end":{"value":293,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29108"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35789156"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1E"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1F"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1G"}],"ligand":{"name":"Ca(2+)","id":"ChEBI:CHEBI:29108","note":"ligand shared between two neighboring subunits"}},{"type":"Binding site","location":{"start":{"value":296,"modifier":"EXACT"},"end":{"value":296,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29108"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35789156"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1E"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1F"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1G"}],"ligand":{"name":"Ca(2+)","id":"ChEBI:CHEBI:29108","note":"ligand shared between two neighboring subunits"}},{"type":"Binding site","location":{"start":{"value":301,"modifier":"EXACT"},"end":{"value":301,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29108"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35789156"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1E"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1F"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1G"}],"ligand":{"name":"Ca(2+)","id":"ChEBI:CHEBI:29108","note":"ligand shared between two neighboring subunits"}},{"type":"Binding site","location":{"start":{"value":304,"modifier":"EXACT"},"end":{"value":304,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29108"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35789156"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1E"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1F"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1G"}],"ligand":{"name":"Ca(2+)","id":"ChEBI:CHEBI:29108","note":"ligand shared between two neighboring subunits"}},{"type":"Mutagenesis","location":{"start":{"value":299,"modifier":"EXACT"},"end":{"value":299,"modifier":"EXACT"}},"description":"Does not affect expression level. Does not affect interaction with GLUL. Does not affect tethering of GLUL to the membrane. Impairs channel activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36289327"}],"alternativeSequence":{"originalSequence":"G","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":304,"modifier":"EXACT"},"end":{"value":304,"modifier":"EXACT"}},"description":"Does not affect expression level. Does not affect interaction with GLUL. Does not affect tethering of GLUL to the membrane. Impairs channel activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36289327"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["A"]}},{"type":"Sequence conflict","location":{"start":{"value":388,"modifier":"EXACT"},"end":{"value":388,"modifier":"EXACT"}},"description":"in Ref. 2; AAR99655 and 3; BAA90970","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["G"]}},{"type":"Helix","location":{"start":{"value":7,"modifier":"EXACT"},"end":{"value":9,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8D1E"}]},{"type":"Helix","location":{"start":{"value":16,"modifier":"EXACT"},"end":{"value":22,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8D1E"}]},{"type":"Helix","location":{"start":{"value":28,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8D1E"}]},{"type":"Helix","location":{"start":{"value":49,"modifier":"EXACT"},"end":{"value":53,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8D1E"}]},{"type":"Helix","location":{"start":{"value":56,"modifier":"EXACT"},"end":{"value":70,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8D1E"}]},{"type":"Helix","location":{"start":{"value":75,"modifier":"EXACT"},"end":{"value":98,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8D1E"}]},{"type":"Helix","location":{"start":{"value":104,"modifier":"EXACT"},"end":{"value":113,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8D1E"}]},{"type":"Helix","location":{"start":{"value":119,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8D1E"}]},{"type":"Helix","location":{"start":{"value":145,"modifier":"EXACT"},"end":{"value":150,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8D1E"}]},{"type":"Beta strand","location":{"start":{"value":151,"modifier":"EXACT"},"end":{"value":153,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8D1F"}]},{"type":"Helix","location":{"start":{"value":154,"modifier":"EXACT"},"end":{"value":159,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8D1E"}]},{"type":"Helix","location":{"start":{"value":165,"modifier":"EXACT"},"end":{"value":173,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8D1E"}]},{"type":"Helix","location":{"start":{"value":183,"modifier":"EXACT"},"end":{"value":198,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8D1E"}]},{"type":"Helix","location":{"start":{"value":204,"modifier":"EXACT"},"end":{"value":229,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8D1E"}]},{"type":"Helix","location":{"start":{"value":234,"modifier":"EXACT"},"end":{"value":254,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8D1E"}]},{"type":"Helix","location":{"start":{"value":256,"modifier":"EXACT"},"end":{"value":259,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8D1E"}]},{"type":"Helix","location":{"start":{"value":260,"modifier":"EXACT"},"end":{"value":262,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8D1E"}]},{"type":"Helix","location":{"start":{"value":275,"modifier":"EXACT"},"end":{"value":294,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8D1E"}]},{"type":"Beta strand","location":{"start":{"value":299,"modifier":"EXACT"},"end":{"value":301,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8D1E"}]},{"type":"Helix","location":{"start":{"value":307,"modifier":"EXACT"},"end":{"value":319,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8D1E"}]},{"type":"Helix","location":{"start":{"value":320,"modifier":"EXACT"},"end":{"value":324,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8D1E"}]},{"type":"Turn","location":{"start":{"value":325,"modifier":"EXACT"},"end":{"value":327,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8D1F"}]},{"type":"Helix","location":{"start":{"value":329,"modifier":"EXACT"},"end":{"value":331,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8D1E"}]},{"type":"Turn","location":{"start":{"value":336,"modifier":"EXACT"},"end":{"value":339,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8D1E"}]},{"type":"Turn","location":{"start":{"value":349,"modifier":"EXACT"},"end":{"value":351,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8D1E"}]},{"type":"Helix","location":{"start":{"value":352,"modifier":"EXACT"},"end":{"value":354,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8D1E"}]},{"type":"Helix","location":{"start":{"value":358,"modifier":"EXACT"},"end":{"value":360,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9DYK"}]},{"type":"Turn","location":{"start":{"value":363,"modifier":"EXACT"},"end":{"value":366,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8D1E"}]},{"type":"Helix","location":{"start":{"value":372,"modifier":"EXACT"},"end":{"value":374,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8D1E"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0106","category":"Ligand","name":"Calcium"},{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0868","category":"Ligand","name":"Chloride"},{"id":"KW-0869","category":"Molecular function","name":"Chloride channel"},{"id":"KW-0407","category":"Molecular function","name":"Ion channel"},{"id":"KW-0406","category":"Biological process","name":"Ion transport"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-1267","category":"Technical term","name":"Proteomics identification"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"12032738","citationType":"journal article","authors":["Stoehr H.","Marquardt A.","Nanda I.","Schmid M.","Weber B.H.F."],"citationCrossReferences":[{"database":"PubMed","id":"12032738"},{"database":"DOI","id":"10.1038/sj.ejhg.5200796"}],"title":"Three novel human VMD2-like genes are members of the evolutionary highly conserved RFP-TM family.","publicationDate":"2002","journal":"Eur. J. Hum. Genet.","firstPage":"281","lastPage":"284","volume":"10"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]","TISSUE SPECIFICITY"]},{"referenceNumber":2,"citation":{"id":"12907679","citationType":"journal article","authors":["Tsunenari T.","Sun H.","Williams J.","Cahill H.","Smallwood P.","Yau K.-W.","Nathans J."],"citationCrossReferences":[{"database":"PubMed","id":"12907679"},{"database":"DOI","id":"10.1074/jbc.m306150200"}],"title":"Structure-function analysis of the bestrophin family of anion channels.","publicationDate":"2003","journal":"J. Biol. Chem.","firstPage":"41114","lastPage":"41125","volume":"278"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]"]},{"referenceNumber":3,"citation":{"id":"14702039","citationType":"journal article","authors":["Ota T.","Suzuki Y.","Nishikawa T.","Otsuki T.","Sugiyama T.","Irie R.","Wakamatsu A.","Hayashi K.","Sato H.","Nagai K.","Kimura K.","Makita H.","Sekine M.","Obayashi M.","Nishi T.","Shibahara T.","Tanaka T.","Ishii S.","Yamamoto J.","Saito K.","Kawai Y.","Isono Y.","Nakamura Y.","Nagahari K.","Murakami K.","Yasuda T.","Iwayanagi T.","Wagatsuma M.","Shiratori A.","Sudo H.","Hosoiri T.","Kaku Y.","Kodaira H.","Kondo H.","Sugawara M.","Takahashi M.","Kanda K.","Yokoi T.","Furuya T.","Kikkawa E.","Omura Y.","Abe K.","Kamihara K.","Katsuta N.","Sato K.","Tanikawa M.","Yamazaki M.","Ninomiya K.","Ishibashi T.","Yamashita H.","Murakawa K.","Fujimori K.","Tanai H.","Kimata M.","Watanabe M.","Hiraoka S.","Chiba Y.","Ishida S.","Ono Y.","Takiguchi S.","Watanabe S.","Yosida M.","Hotuta T.","Kusano J.","Kanehori K.","Takahashi-Fujii A.","Hara H.","Tanase T.-O.","Nomura Y.","Togiya S.","Komai F.","Hara R.","Takeuchi K.","Arita M.","Imose N.","Musashino K.","Yuuki H.","Oshima A.","Sasaki N.","Aotsuka S.","Yoshikawa Y.","Matsunawa H.","Ichihara T.","Shiohata N.","Sano S.","Moriya S.","Momiyama H.","Satoh N.","Takami S.","Terashima Y.","Suzuki O.","Nakagawa S.","Senoh A.","Mizoguchi H.","Goto Y.","Shimizu F.","Wakebe H.","Hishigaki H.","Watanabe T.","Sugiyama A.","Takemoto M.","Kawakami B.","Yamazaki M.","Watanabe K.","Kumagai A.","Itakura S.","Fukuzumi Y.","Fujimori Y.","Komiyama M.","Tashiro H.","Tanigami A.","Fujiwara T.","Ono T.","Yamada K.","Fujii Y.","Ozaki K.","Hirao M.","Ohmori Y.","Kawabata A.","Hikiji T.","Kobatake N.","Inagaki H.","Ikema Y.","Okamoto S.","Okitani R.","Kawakami T.","Noguchi S.","Itoh T.","Shigeta K.","Senba T.","Matsumura K.","Nakajima Y.","Mizuno T.","Morinaga M.","Sasaki M.","Togashi T.","Oyama M.","Hata H.","Watanabe M.","Komatsu T.","Mizushima-Sugano J.","Satoh T.","Shirai Y.","Takahashi Y.","Nakagawa K.","Okumura K.","Nagase T.","Nomura N.","Kikuchi H.","Masuho Y.","Yamashita R.","Nakai K.","Yada T.","Nakamura Y.","Ohara O.","Isogai T.","Sugano S."],"citationCrossReferences":[{"database":"PubMed","id":"14702039"},{"database":"DOI","id":"10.1038/ng1285"}],"title":"Complete sequencing and characterization of 21,243 full-length human cDNAs.","publicationDate":"2004","journal":"Nat. Genet.","firstPage":"40","lastPage":"45","volume":"36"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 23-509"],"referenceComments":[{"value":"Colon","type":"TISSUE"}]},{"referenceNumber":4,"citation":{"id":"11904445","citationType":"journal article","authors":["Sun H.","Tsunenari T.","Yau K.-W.","Nathans J."],"citationCrossReferences":[{"database":"PubMed","id":"11904445"},{"database":"DOI","id":"10.1073/pnas.052692999"}],"title":"The vitelliform macular dystrophy protein defines a new family of chloride channels.","publicationDate":"2002","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"4008","lastPage":"4013","volume":"99"},"referencePositions":["FUNCTION","TRANSPORTER ACTIVITY"]},{"referenceNumber":5,"citation":{"id":"18400985","citationType":"journal article","authors":["Qu Z.","Hartzell H.C."],"citationCrossReferences":[{"database":"PubMed","id":"18400985"},{"database":"DOI","id":"10.1152/ajpcell.00398.2007"}],"title":"Bestrophin Cl- channels are highly permeable to HCO3-.","publicationDate":"2008","journal":"Am. J. Physiol.","firstPage":"C1371","lastPage":"C1377","volume":"294"},"referencePositions":["FUNCTION","TRANSPORTER ACTIVITY"]},{"referenceNumber":6,"citation":{"id":"20407206","citationType":"journal article","authors":["Yu K.","Lujan R.","Marmorstein A.","Gabriel S.","Hartzell H.C."],"citationCrossReferences":[{"database":"PubMed","id":"20407206"},{"database":"DOI","id":"10.1172/jci41129"}],"title":"Bestrophin-2 mediates bicarbonate transport by goblet cells in mouse colon.","publicationDate":"2010","journal":"J. Clin. Invest.","firstPage":"1722","lastPage":"1735","volume":"120"},"referencePositions":["SUBCELLULAR LOCATION","TISSUE SPECIFICITY"]},{"referenceNumber":7,"citation":{"id":"32251414","citationType":"journal article","authors":["Owji A.P.","Zhao Q.","Ji C.","Kittredge A.","Hopiavuori A.","Fu Z.","Ward N.","Clarke O.B.","Shen Y.","Zhang Y.","Hendrickson W.A.","Yang T."],"citationCrossReferences":[{"database":"PubMed","id":"32251414"},{"database":"DOI","id":"10.1038/s41594-020-0402-z"}],"title":"Structural and functional characterization of the bestrophin-2 anion channel.","publicationDate":"2020","journal":"Nat. Struct. Mol. Biol.","firstPage":"382","lastPage":"391","volume":"27"},"referencePositions":["FUNCTION","TRANSPORTER ACTIVITY"]},{"referenceNumber":8,"citation":{"id":"36289327","citationType":"journal article","authors":["Owji A.P.","Yu K.","Kittredge A.","Wang J.","Zhang Y.","Yang T."],"citationCrossReferences":[{"database":"PubMed","id":"36289327"},{"database":"DOI","id":"10.1038/s41586-022-05373-x"}],"title":"Bestrophin-2 and glutamine synthetase form a complex for glutamate release.","publicationDate":"2022","journal":"Nature","firstPage":"180","lastPage":"187","volume":"611"},"referencePositions":["INTERACTION WITH GLUL","FUNCTION","TRANSPORTER ACTIVITY","MUTAGENESIS OF GLY-299 AND ASP-304"]},{"referenceNumber":9,"citation":{"id":"35789156","citationType":"journal article","authors":["Owji A.P.","Wang J.","Kittredge A.","Clark Z.","Zhang Y.","Hendrickson W.A.","Yang T."],"citationCrossReferences":[{"database":"PubMed","id":"35789156"},{"database":"DOI","id":"10.1038/s41467-022-31437-7"}],"title":"Structures and gating mechanisms of human bestrophin anion channels.","publicationDate":"2022","journal":"Nat. Commun.","firstPage":"3836","lastPage":"3836","volume":"13"},"referencePositions":["STRUCTURE BY ELECTRON MICROSCOPY (1.78 ANGSTROMS) OF 1-406 IN COMPLEX WITH CALCIUM AND CHLORIDE","FUNCTION","CATALYTIC ACTIVITY","DOMAIN","REGION","SUBUNIT","TOPOLOGY"],"evidences":[{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1E"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1F"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1G"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"8D1H"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF440756","properties":[{"key":"ProteinId","value":"AAM76995.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AY515705","properties":[{"key":"ProteinId","value":"AAR99655.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK000139","properties":[{"key":"ProteinId","value":"BAA90970.1"},{"key":"Status","value":"ALT_INIT"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"CCDS","id":"CCDS42506.1","properties":[{"key":"Description","value":"-"}]},{"database":"RefSeq","id":"NP_060152.2","properties":[{"key":"NucleotideSequenceId","value":"NM_017682.3"}]},{"database":"PDB","id":"8D1E","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"1.78 A"},{"key":"Chains","value":"A/B/C/D/E=1-406"}]},{"database":"PDB","id":"8D1F","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"1.82 A"},{"key":"Chains","value":"A/B/C/D/E=1-406"}]},{"database":"PDB","id":"8D1G","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.07 A"},{"key":"Chains","value":"A/B/C/D/E=1-406"}]},{"database":"PDB","id":"8D1H","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"1.94 A"},{"key":"Chains","value":"A/B/C/D/E=1-406"}]},{"database":"PDB","id":"9DYH","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.15 A"},{"key":"Chains","value":"A/B/C/D/E=1-509"}]},{"database":"PDB","id":"9DYI","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.03 A"},{"key":"Chains","value":"A/B/C/D/E=1-509"}]},{"database":"PDB","id":"9DYJ","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.31 A"},{"key":"Chains","value":"A/B/C/D/E=1-509"}]},{"database":"PDB","id":"9DYK","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.27 A"},{"key":"Chains","value":"A/B/C/D/E=1-406"}]},{"database":"PDB","id":"9DYN","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.44 A"},{"key":"Chains","value":"A/B/C/D/E=1-509"}]},{"database":"PDB","id":"9DYO","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.57 A"},{"key":"Chains","value":"A/B/C/D/E=1-509"}]},{"database":"PDBsum","id":"8D1E","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"8D1F","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"8D1G","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"8D1H","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9DYH","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9DYI","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9DYJ","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9DYK","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9DYN","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9DYO","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"Q8NFU1","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-27127","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-27128","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-27129","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-27130","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-47304","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-47305","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-47306","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-47307","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-47310","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-47311","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q8NFU1","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"120185","properties":[{"key":"Interactions","value":"17"}]},{"database":"FunCoup","id":"Q8NFU1","properties":[{"key":"Number of interactors","value":"36"}]},{"database":"IntAct","id":"Q8NFU1","properties":[{"key":"Interactions","value":"16"}]},{"database":"STRING","id":"9606.ENSP00000448310","properties":[{"key":"Description","value":"-"}]},{"database":"TCDB","id":"1.A.46.1.2","properties":[{"key":"FamilyName","value":"the anion channel-forming bestrophin (bestrophin) family"}]},{"database":"GlyGen","id":"Q8NFU1","properties":[{"key":"glycosylation","value":"1 site, 1 N-linked glycan (1 site)"}]},{"database":"iPTMnet","id":"Q8NFU1","properties":[{"key":"Description","value":"-"}]},{"database":"PhosphoSitePlus","id":"Q8NFU1","properties":[{"key":"Description","value":"-"}]},{"database":"BioMuta","id":"BEST2","properties":[{"key":"Description","value":"-"}]},{"database":"DMDM","id":"38503353","properties":[{"key":"Description","value":"-"}]},{"database":"MassIVE","id":"Q8NFU1","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"9606-ENSP00000448310","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"Q8NFU1","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"73356","properties":[{"key":"Description","value":"-"}]},{"database":"TopDownProteomics","id":"Q8NFU1","properties":[{"key":"Description","value":"-"}]},{"database":"Antibodypedia","id":"26152","properties":[{"key":"antibodies","value":"147 antibodies from 25 providers"}]},{"database":"DNASU","id":"54831","properties":[{"key":"Description","value":"-"}]},{"database":"Ensembl","id":"ENST00000042931.1","properties":[{"key":"ProteinId","value":"ENSP00000042931.1"},{"key":"GeneId","value":"ENSG00000039987.8"}]},{"database":"Ensembl","id":"ENST00000549706.5","properties":[{"key":"ProteinId","value":"ENSP00000448310.1"},{"key":"GeneId","value":"ENSG00000039987.8"}]},{"database":"Ensembl","id":"ENST00000553030.6","properties":[{"key":"ProteinId","value":"ENSP00000447203.1"},{"key":"GeneId","value":"ENSG00000039987.8"}]},{"database":"GeneID","id":"54831","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"hsa:54831","properties":[{"key":"Description","value":"-"}]},{"database":"MANE-Select","id":"ENST00000553030.6","properties":[{"key":"ProteinId","value":"ENSP00000447203.1"},{"key":"RefSeqNucleotideId","value":"NM_017682.3"},{"key":"RefSeqProteinId","value":"NP_060152.2"}]},{"database":"UCSC","id":"uc002mux.4","properties":[{"key":"OrganismName","value":"human"}]},{"database":"AGR","id":"HGNC:17107","properties":[{"key":"Description","value":"-"}]},{"database":"ClinPGx","id":"PA162377481","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"54831","properties":[{"key":"Description","value":"-"}]},{"database":"DisGeNET","id":"54831","properties":[{"key":"Description","value":"-"}]},{"database":"GeneCards","id":"BEST2","properties":[{"key":"Description","value":"-"}]},{"database":"HGNC","id":"HGNC:17107","properties":[{"key":"GeneName","value":"BEST2"}]},{"database":"HPA","id":"ENSG00000039987","properties":[{"key":"ExpressionPatterns","value":"Group enriched (intestine, skin)"}]},{"database":"MIM","id":"607335","properties":[{"key":"Type","value":"gene"}]},{"database":"OpenTargets","id":"ENSG00000039987","properties":[{"key":"Description","value":"-"}]},{"database":"VEuPathDB","id":"HostDB:ENSG00000039987","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG3547","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"GeneTree","id":"ENSGT00940000161361","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_018069_0_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q8NFU1","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"APECGCG","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"201595at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PAN-GO","id":"Q8NFU1","properties":[{"key":"Number of GO annotations","value":"3 GO annotations based on evolutionary models"}]},{"database":"PhylomeDB","id":"Q8NFU1","properties":[{"key":"Description","value":"-"}]},{"database":"PathwayCommons","id":"Q8NFU1","properties":[{"key":"Description","value":"-"}]},{"database":"Reactome","id":"R-HSA-2672351","properties":[{"key":"PathwayName","value":"Stimuli-sensing channels"}]},{"database":"SignaLink","id":"Q8NFU1","properties":[{"key":"Description","value":"-"}]},{"database":"Agora","id":"ENSG00000039987","properties":[{"key":"NominatedTarget","value":"-"}]},{"database":"BioGRID-ORCS","id":"54831","properties":[{"key":"hits","value":"12 hits in 1141 CRISPR screens"}]},{"database":"GeneWiki","id":"BEST2","properties":[{"key":"Description","value":"-"}]},{"database":"GenomeRNAi","id":"54831","properties":[{"key":"Description","value":"-"}]},{"database":"Pharos","id":"Q8NFU1","properties":[{"key":"DevelopmentLevel","value":"Tbio"}]},{"database":"PRO","id":"PR:Q8NFU1","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000005640","properties":[{"key":"Component","value":"Chromosome 19"}]},{"database":"RNAct","id":"Q8NFU1","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"Bgee","id":"ENSG00000039987","properties":[{"key":"ExpressionPatterns","value":"Expressed in mucosa of transverse colon and 101 other cell types or tissues"}]},{"database":"ExpressionAtlas","id":"Q8NFU1","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0016323","properties":[{"key":"GoTerm","value":"C:basolateral plasma membrane"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20407206"}]},{"database":"GO","id":"GO:0034707","properties":[{"key":"GoTerm","value":"C:chloride channel complex"},{"key":"GoEvidenceType","value":"ISS:BHF-UCL"}]},{"database":"GO","id":"GO:0005929","properties":[{"key":"GoTerm","value":"C:cilium"},{"key":"GoEvidenceType","value":"ISS:HGNC-UCL"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0160133","properties":[{"key":"GoTerm","value":"F:bicarbonate channel activity"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"18400985"}]},{"database":"GO","id":"GO:0005254","properties":[{"key":"GoTerm","value":"F:chloride channel activity"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"18400985"}]},{"database":"GO","id":"GO:0005217","properties":[{"key":"GoTerm","value":"F:intracellularly ligand-gated monoatomic ion channel activity"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"36289327"}]},{"database":"GO","id":"GO:0099095","properties":[{"key":"GoTerm","value":"F:ligand-gated monoatomic anion channel activity"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0099094","properties":[{"key":"GoTerm","value":"F:ligand-gated monoatomic cation channel activity"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"35789156"}]},{"database":"GO","id":"GO:1902476","properties":[{"key":"GoTerm","value":"P:chloride transmembrane transport"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0051899","properties":[{"key":"GoTerm","value":"P:membrane depolarization"},{"key":"GoEvidenceType","value":"ISS:HGNC-UCL"}]},{"database":"GO","id":"GO:0007608","properties":[{"key":"GoTerm","value":"P:sensory perception of smell"},{"key":"GoEvidenceType","value":"ISS:HGNC-UCL"}]},{"database":"InterPro","id":"IPR000615","properties":[{"key":"EntryName","value":"Bestrophin"}]},{"database":"InterPro","id":"IPR021134","properties":[{"key":"EntryName","value":"Bestrophin-like"}]},{"database":"PANTHER","id":"PTHR10736","properties":[{"key":"EntryName","value":"BESTROPHIN"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR10736:SF1","properties":[{"key":"EntryName","value":"BESTROPHIN-2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01062","properties":[{"key":"EntryName","value":"Bestrophin"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MTVTYTARVANARFGGFSQLLLLWRGSIYKLLWRELLCFLGFYMALSAAYRFVLTEGQKRYFEKLVIYCDQYASLIPVSFVLGFYVTLVVNRWWSQYLCMPLPDALMCVVAGTVHGRDDRGRLYRRTLMRYAGLSAVLILRSVSTAVFKRFPTIDHVVEAGFMTREERKKFENLNSSYNKYWVPCVWFSNLAAQARREGRIRDNSALKLLLEELNVFRGKCGMLFHYDWISVPLVYTQVVTIALYSYFLACLIGRQFLDPAQGYKDHDLDLCVPIFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNFLIDRNFQVSMLAVDEMYDDLAVLEKDLYWDAAEARAPYTAATVFQLRQPSFQGSTFDITLAKEDMQFQRLDGLDGPMGEAPGDFLQRLLPAGAGMVAGGPLGRRLSFLLRKNSCVSEASTGASCSCAVVPEGAAPECSCGDPLLDPGLPEPEAPPPAGPEPLTLIPGPVEPFSIVTMPGPRGPAPPWLPSPIGEEEENLA","length":509,"molWeight":57139,"crc64":"E557B975BFDC692A","md5":"774FEEA0A170AA1D8E13036C405A16C8"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":5,"ACTIVITY REGULATION":1,"SUBUNIT":1,"INTERACTION":16,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"DOMAIN":1,"SIMILARITY":1,"SEQUENCE CAUTION":1},"countByFeatureType":{"Chain":1,"Topological domain":5,"Transmembrane":4,"Region":1,"Compositional bias":2,"Binding site":5,"Mutagenesis":2,"Sequence conflict":1,"Helix":23,"Beta strand":2,"Turn":4},"uniParcId":"UPI000007395A"}}