{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q8NE86","secondaryAccessions":["B2RDF3","B3KXV7","Q96FL3"],"uniProtkbId":"MCU_HUMAN","entryAudit":{"firstPublicDate":"2007-04-03","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2002-10-01","entryVersion":170,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Homo sapiens","commonName":"Human","taxonId":9606,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Primates","Haplorrhini","Catarrhini","Hominidae","Homo"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"21685886"}],"value":"Calcium uniporter protein, mitochondrial"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"27135929"}],"value":"HsMCU"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Coiled-coil domain-containing protein 109A"}}],"flag":"Precursor"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"21685886"},{"evidenceCode":"ECO:0000312","source":"HGNC","id":"HGNC:23526"}],"value":"MCU"},"synonyms":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"HGNC","id":"HGNC:23526"}],"value":"C10orf42"},{"evidences":[{"evidenceCode":"ECO:0000312","source":"HGNC","id":"HGNC:23526"}],"value":"CCDC109A"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q3UMR5"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21685886"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21685888"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22822213"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22829870"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22904319"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23101630"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23178883"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23755363"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24332854"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24560927"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25603276"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26341627"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26903221"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29954988"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29995857"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30454562"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30638448"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31080062"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32494073"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32762847"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33296646"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37036971"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37126688"}],"value":"Channel-forming and calcium-conducting subunit of the mitochondrial inner membrane calcium uniporter complex (uniplex), which mediates calcium uptake into the mitochondrial matrix (PubMed:21685886, PubMed:21685888, PubMed:22822213, PubMed:22829870, PubMed:22904319, PubMed:23101630, PubMed:23178883, PubMed:23755363, PubMed:24332854, PubMed:24560927, PubMed:26341627, PubMed:29954988, PubMed:29995857, PubMed:30454562, PubMed:30638448, PubMed:31080062, PubMed:32494073, PubMed:32762847, PubMed:33296646, PubMed:37036971, PubMed:37126688). MCU channel activity is regulated by the calcium-sensor subunits of the uniplex MICU1 and MICU2 (or MICU3) (PubMed:24560927, PubMed:26903221, PubMed:30454562, PubMed:30638448, PubMed:32494073, PubMed:32762847, PubMed:37036971, PubMed:37126688). Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates ATP production, cytoplasmic calcium signals and activation of cell death pathways (PubMed:21685886, PubMed:21685888, PubMed:22822213, PubMed:22829870, PubMed:22904319, PubMed:23101630, PubMed:23178883, PubMed:23755363, PubMed:24332854, PubMed:24560927, PubMed:26341627, PubMed:29954988, PubMed:32494073, PubMed:32762847). Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes (PubMed:22822213). While dispensable for baseline homeostatic cardiac function, acts as a key regulator of short-term mitochondrial calcium loading underlying a 'fight-or-flight' response during acute stress: acts by mediating a rapid increase of mitochondrial calcium in pacemaker cells (PubMed:25603276). Participates in mitochondrial permeability transition during ischemia-reperfusion injury (By similarity). Mitochondrial calcium uptake in skeletal muscle cells is involved in muscle size in adults (By similarity). Regulates synaptic vesicle endocytosis kinetics in central nerve terminal (By similarity). Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake (PubMed:22829870, PubMed:22904319). Involved in antigen processing and presentation (By similarity)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Ca(2+)(in) = Ca(2+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:29671"},{"database":"ChEBI","id":"CHEBI:29108"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21685888"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24560927"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29954988"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29995857"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30638448"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31080062"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32494073"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33296646"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23755363"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24560927"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26903221"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31080062"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32494073"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32762847"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33296646"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37036971"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37126688"}],"value":"MCU channel activity is regulated by the heterodimer composed of MICU1 and either MICU2 or MICU3, which act as calcium-sensors (PubMed:24560927, PubMed:26903221, PubMed:32494073, PubMed:32762847, PubMed:37036971, PubMed:37126688). At low calcium levels, MICU1 occludes the pore of the MCU channel, preventing mitochondrial calcium uptake (PubMed:32494073, PubMed:32762847, PubMed:37036971, PubMed:37126688). At higher calcium levels, calcium-binding to MICU1 and MICU2 (or MICU3) induces a conformational change that weakens MCU-MICU1 interactions and moves the MICU1-MICU2 heterodimer away from the pore, allowing calcium permeation through the channel (PubMed:32494073, PubMed:32762847). MCU channel activity is gated by EMRE/SMDT1 via the juxtamembrane helix loop (PubMed:31080062, PubMed:33296646). Inhibited by ruthenium red or its derivative Ru360 (PubMed:23755363, PubMed:37036971, PubMed:37126688)"}],"commentType":"ACTIVITY REGULATION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23178883"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24231807"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24430870"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26341627"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26976564"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27184846"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31080062"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32494073"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32862359"}],"value":"Homotetramer (PubMed:32494073). Component of the uniplex complex, composed of MCU, EMRE/SMDT1, MICU1 and MICU2 (or MICU3) in a 4:4:1:1 stoichiometry (PubMed:24231807, PubMed:26341627, PubMed:31080062, PubMed:32494073, PubMed:32862359). Interacts with CCDC109B/MCUB; this inhibits channel activity (PubMed:27184846). Interacts with MCUR1 (PubMed:23178883, PubMed:26341627, PubMed:26976564, PubMed:27184846). Interactions with MICU1 and MCUR1 are mutually exclusive (PubMed:23178883). Interacts with SLC25A23 (PubMed:24430870)"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"Q8NE86","intActId":"EBI-6552124"},"interactantTwo":{"uniProtKBAccession":"Q9BPX6","geneName":"MICU1","intActId":"EBI-2371996"},"numberOfExperiments":7,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q8NE86","intActId":"EBI-6552124"},"interactantTwo":{"uniProtKBAccession":"Q9H4I9","geneName":"SMDT1","intActId":"EBI-11908005"},"numberOfExperiments":8,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q8NE86-1","intActId":"EBI-15932889"},"interactantTwo":{"uniProtKBAccession":"Q8NE86-1","geneName":"MCU","intActId":"EBI-15932889"},"numberOfExperiments":2,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q8NE86-1","intActId":"EBI-15932889"},"interactantTwo":{"uniProtKBAccession":"Q96AQ8","geneName":"MCUR1","intActId":"EBI-14404755"},"numberOfExperiments":4,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q8NE86-1","intActId":"EBI-15932889"},"interactantTwo":{"uniProtKBAccession":"Q9BPX6","geneName":"MICU1","intActId":"EBI-2371996"},"numberOfExperiments":2,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q8NE86-1","intActId":"EBI-15932889"},"interactantTwo":{"uniProtKBAccession":"Q9BPX6-1","geneName":"MICU1","intActId":"EBI-5456336"},"numberOfExperiments":2,"organismDiffer":false}]},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21685886"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21685888"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23755363"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24231807"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27099988"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"26341627"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"31080062"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21685886"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21685888"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24231807"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31080062"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32494073"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32762847"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32862359"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"commentType":"ALTERNATIVE PRODUCTS","events":["Alternative splicing"],"isoforms":[{"name":{"value":"1"},"isoformIds":["Q8NE86-1"],"isoformSequenceStatus":"Displayed"},{"name":{"value":"2"},"isoformIds":["Q8NE86-2"],"sequenceIds":["VSP_024263"],"isoformSequenceStatus":"Described"},{"name":{"value":"3"},"isoformIds":["Q8NE86-3"],"sequenceIds":["VSP_041687"],"isoformSequenceStatus":"Described"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23246404"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25764156"}],"value":"MCU transcripts are down-regulated by microRNA miR-25 (PubMed:23246404). Down-regulation by miR-25 may protect cardiomyocytes against oxidative damage in cardiomyocytes (PubMed:25764156)"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32494073"}],"value":"The selectivity filter, in which calcium ions are arranged in single file, is composed of two acidic rings separated by one helical turn along the central axis of the channel pore"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26341627"}],"value":"The N-terminal MCU domain is required for efficient Ca(2+) uptake and for interaction with MCUR1 (PubMed:26341627). It is not required for targeting to the mitochondria, oligomerization, interaction with MICU1 and MICU2, or assembly of the uniplex complex (PubMed:26341627)"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24800979"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"23051746"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"25254480"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"25254481"}],"value":"Phosphorylation by CaMK2 in heart leads to increased MCU current (PubMed:23051746, PubMed:25254481). The regulation of MCU by CaMK2 is however subject to discussion: another group was unable to reproduce these results (PubMed:25254480). Phosphorylated on tyrosines by PTK2B/PYK2, promoting oligomerization (PubMed:24800979)"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28262504"}],"value":"Glutathionylation at Cys-97 in response to reactive oxygen species (ROS) promotes MCU higher-order assembly, leading to constitutive activation of the MCU channel and mitochondrial calcium overload"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31097542"}],"value":"Undergoes proteolytic degradation by SPG7"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the MCU (TC 1.A.77) family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transit peptide","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":50,"modifier":"EXACT"}},"description":"Mitochondrion","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Chain","location":{"start":{"value":51,"modifier":"EXACT"},"end":{"value":351,"modifier":"EXACT"}},"description":"Calcium uniporter protein, mitochondrial","featureId":"PRO_0000282976"},{"type":"Topological domain","location":{"start":{"value":51,"modifier":"EXACT"},"end":{"value":233,"modifier":"EXACT"}},"description":"Mitochondrial matrix","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"27099988"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"31080062"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"32494073"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"32762847"}]},{"type":"Transmembrane","location":{"start":{"value":234,"modifier":"EXACT"},"end":{"value":255,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31080062"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32494073"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32762847"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32862359"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"6K7X"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"6K7Y"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"6O58"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"6O5B"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"6WDN"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"6WDO"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"6XJV"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"6XJX"}]},{"type":"Topological 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intermembrane","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"27099988"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"31080062"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"32494073"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"32762847"}]},{"type":"Transmembrane","location":{"start":{"value":263,"modifier":"EXACT"},"end":{"value":284,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31080062"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32494073"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32762847"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32862359"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"6K7X"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"6K7Y"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"6O58"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"6O5B"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"6WDN"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"6WDO"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"6XJV"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"6XJX"}]},{"type":"Topological domain","location":{"start":{"value":285,"modifier":"EXACT"},"end":{"value":351,"modifier":"EXACT"}},"description":"Mitochondrial matrix","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23755363"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"27099988"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"31080062"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"32494073"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"32762847"}]},{"type":"Region","location":{"start":{"value":75,"modifier":"EXACT"},"end":{"value":165,"modifier":"EXACT"}},"description":"N-terminal MCU domain","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26341627"}]},{"type":"Region","location":{"start":{"value":285,"modifier":"EXACT"},"end":{"value":290,"modifier":"EXACT"}},"description":"Juxtamembrane helix","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31080062"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33296646"}]},{"type":"Coiled coil","location":{"start":{"value":192,"modifier":"EXACT"},"end":{"value":223,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Coiled coil","location":{"start":{"value":311,"modifier":"EXACT"},"end":{"value":339,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Motif","location":{"start":{"value":260,"modifier":"EXACT"},"end":{"value":268,"modifier":"EXACT"}},"description":"Selectivity filter","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31080062"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32494073"}]},{"type":"Binding site","location":{"start":{"value":264,"modifier":"EXACT"},"end":{"value":264,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29108"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31080062"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32494073"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32862359"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"6K7X"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"6K7Y"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"6O58"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"6O5B"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"6WDO"}],"ligand":{"name":"Ca(2+)","id":"ChEBI:CHEBI:29108"}},{"type":"Modified residue","location":{"start":{"value":57,"modifier":"EXACT"},"end":{"value":57,"modifier":"EXACT"}},"description":"Phosphoserine; by CaMK2","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"23051746"}]},{"type":"Modified 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3","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"14702039"}],"featureId":"VSP_041687","alternativeSequence":{"originalSequence":"MAAAAGRSLLLLLSSRGGGGGGAGGCGALTAGCFPGLGVSRHRQQQHHRT","alternativeSequences":["M"]}},{"type":"Alternative sequence","location":{"start":{"value":166,"modifier":"EXACT"},"end":{"value":219,"modifier":"EXACT"}},"description":"in isoform 2","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"15489334"}],"featureId":"VSP_024263","alternativeSequence":{"originalSequence":"DLLSHENAATLNDVKTLVQQLYTTLCIEQHQLNKERELIERLEDLKEQLAPLEK","alternativeSequences":["VEMGFCHVGQNGFELLTSSYLPASASQSAEIIA"]}},{"type":"Mutagenesis","location":{"start":{"value":57,"modifier":"EXACT"},"end":{"value":57,"modifier":"EXACT"}},"description":"Decreased MCU current; when associated with A-92.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23051746"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":66,"modifier":"EXACT"},"end":{"value":66,"modifier":"EXACT"}},"description":"Does not affect glutathionylation in response to reactive oxygen species.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28262504"}],"alternativeSequence":{"originalSequence":"C","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":92,"modifier":"EXACT"},"end":{"value":92,"modifier":"EXACT"}},"description":"Decreased MCU current; when associated with A-57.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23051746"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":92,"modifier":"EXACT"},"end":{"value":92,"modifier":"EXACT"}},"description":"Impairs calcium uptake, but has no effect on oligomerization and interaction with MICU1 and MICU2.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26341627"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":97,"modifier":"EXACT"},"end":{"value":97,"modifier":"EXACT"}},"description":"Abolished glutathionylation in response to reactive oxygen species.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28262504"}],"alternativeSequence":{"originalSequence":"C","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":123,"modifier":"EXACT"},"end":{"value":123,"modifier":"EXACT"}},"description":"No effect on calcium uptake in presence of high concentrations of calcium. 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